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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 986 Documents
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Influence of Porang (Amorphophalus muelleri) Cultivation On The Composition of Soil Arthropods In Tropical Agroforestry Areas In East Java, Indonesia Leksono, Amin setyo; Yanuwiadi, Bagyo; Zaenal, Kusuma; Farid, Akbar; Maulana, Fujianor
Journal of Tropical Life Science Vol 1, No 2 (2011)
Publisher : Journal of Tropical Life Science

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Abstract

This research was conducted on July to October 2010 to evaluate the effect of porang cultivation on the abundance, diversity and composition of soil Arthropods. Change in the abundance, diversity and composition of soil Arthropods was observed using pitfall traps in two study sites (Jember and Madiun). In each study site, samplings were performed in porang cultivation land and non-porang area. A total of 15 traps were put in three lines at each location. The differences in the abundance and diversity were analyzed by using multivariate analyze tests. The soil Arthropod compositions in all locations were compared with Bray-Curtis similarity index. The effect of environmental factors on the arthropod compositions was analyzed using multiple regression. The result showed that the effect of porang-cultivation was not consistent in two study sites. In Madiun, the abundance of soil Arthropods in porang cultivation land was slightly higher than that in non-porang, while the diversity was significantly higer in the former location. In contrast the those in Jember was found in vice versa situation. This study indicate that the effect of porang-cultivation was not subtantial to the composition of soil Arthropods. Therefore practice non harmful porang cultivation should be promoted as a part of agroforestry sistem in both study sites.Keywords: Agroforestry, conservation, pitfall traps Soil Arthropods composition
Study on The Starch Granules Morphology of Local Varieties of Dioscorea hispida and Dioscorea alata Fauziah, Fauziah; Mas'udah, Shofiyatul; Hendrian, Hendrian
Journal of Tropical Life Science Vol 6, No 1 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.01.09

Abstract

Starch is regarded as vital in the food industry, where granule size and shape determine its function and uses. One plant taxon that is widely known as a starch source is Dioscorea (Dioscoreaceae), whose starch and protein content make it a healthy food. As the initial step to identify which varieties possess potential as starch sources, we conducted study on starch granule morphology of some local varieties of D. hispida and D. alata. The aim of this study was to determine shape and size variation of starch granule of each variety of D. hispida and D. alata. The results showed that starch granule morphology does not only vary between species of D. hispida and D. alata, but also among varieties of those species. The shape of starch granules in D. hispida is dominantly polygonal (polyhedral). It showed similar results as those reported by other authors in their studies, except that – in this study - we found there is also a triangular shape, which has never been reported before. Starch granule size of D. hispida is found to be “Very small – Small” and only 1 variety of the species – that is gadung jahe – is classified as “Small” type. According to previous studies, varieties with small type starch granule are recommended as potential sources for non-food industrial raw materials. The shape of starch granules in D. alata is similar to those reported in other studies, which is dominantly triangular and polygonal; the size is classified as “Medium” type, except for two varieties that are uwi ratu and uwi ulo (DC5), which are classified as “Large”. According to previous studies, varieties with large type starch granule are recommended as potential sources for food. Thus, from this study 3 potential local varieties are identified, i.e. gadung jahe of D. hispida, and uwi ratu & uwi ulo of D. alata.
The Technic of Agroforestry at Communal Forest Areas in Pejarakan Village, District of Gerokgak, Regency of Buleleng, Province of Bali Ardhana, I putu Gede; Sunardi, Sunardi
Journal of Tropical Life Science Vol 3, No 1 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

Since 2003 year the technic of agroforestry with alley cropping systems has been implemented at the forest areas in Pejarakan Village, District Gerokgak, Regency of Buleleng Province of Bali. These activities are expected to support the progress of welfare for farmers, eventhough they have been neglected to preserve forest around village. After the research it was cleared that the result of activities didnt fulfilled to its expectation. The analysis about the compositions and structures of vegetations in this research shows that the dominant type of species which are mainly cultivated have character of Importance Value Index, the balance of species among Diversity Index are still low at all of the communal forest areas and even at the these slope sides. The composition and the structure of species were still few, and the vegetation itself was disturbed by people, in order to illegal loggings, cuttings top branches or twigs often occur, and these activities bring out obstacle growth of trees in communal forest areas. It is concluded that the technic of agroforestry at the communal forest areas has few compositions of species and structure of vegetations, so the diversity of species become very poor and not stable either for plants in forestry or for agricultural crops. The researcher could not determine that what species of vegetations superior at this area. So it necessary to enough time for intensive observations and research at this communal forest areas.
Cross Reaction among Antibody Pili sub unit Hemagglutinin Proteins and Outer Membrane sub unit Hemagglutinin Proteins of Shigella flexneri Fitrianingsih, Avin Ainur; Rachma, Lailia Nur; Milliana, Alvi; Hernowati, Tinny Endang; Aulanni'am, Aulanni'am; Santoso, Sanarto; Prawiro, Sumarno Reto
Journal of Tropical Life Science Vol 7, No 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.01

Abstract

Shigella  flexneri is the most common causal agent of shigellosis. Its pili are composed of pili protein subunits. Adhesion molecules can be found on the pili and outer membrane proteins (Omp). A hemagglutination reaction can be used for screening of adhesion molecules. Objectives: The purpose of this study was to determine the molecular weight of the pili protein subunits and outer membrane proteins of S. flexneri that act as hemagglutinin proteins, and to prove whether there is a cross-reaction between antibodies against hemagglutinin pili protein subunits and outer membrane proteins of S. flexneri. Methods: Pili protein subunits were isolated using pili bacteria cutters, and the outer membrane proteins were solubilized and obtained using sodium dodecyl sulfate 0.05% as detergent for Omp isolation.  The hemagglutination reaction used mice erythrocytes. The cross reactions between subunit pili proteins were conducted by Western blot and Dot blot. Results:. Antibodies against hemagglutinin sub unit pili protein 18 kDa responded to pili protein subunits 18 kDa; 23 kDa; 34 kDa; and 53 kDa; and Omp 23 kDa and 27 kDa. Omp and subunit pili proteins S. flexneri consists of several identical epitopes  that were responsible for the similarity of the response profile in the cross-reactions of antibodies. 
Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Agustina, Dini; Retoprawiro, Sumarno; AS, Noorhamdani
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

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Abstract

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.
Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Tanor, Meity N; Abadi, Abdul Latief; Rahardjo, Bambang Tri; Pelealu, Jantje
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

Abstract

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).
Potential Antibacterials Compounds of Lactic Acid Bacteria (LAB) from Quail Intestine (Coturnix japonica) in Inhibition Growth of Escherichia coli and Salmonella typhimurium Afdora, Pupimadita Tizar; Ardiyati, Tri; Sjofjan, Osfar; Kalsum, Umi
Journal of Tropical Life Science Vol 1, No 1 (2010)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.1.1.%x

Abstract

Quail (Coturnix japonica) is a bird that have high protein content, but vulnerable to digestive diseases. The purpose of this research was to determine the ability of antibacterial compounds of LAB from intestinal quail origin in bacterial growth inhibition test. This research used Completely Randomized Design (CRD) with variable concentrations of Cell Free Supernatant (CFS) for 10, 20, 30, 40, 50, and 60% and bacterial pathogens of the digestive tract of quail (S. typhimurium, E. coli of human origin, and E. coli of bird origin) by using the Minimum inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC). The data obtained were analyzed by Analysis of Variance (ANOVA). The results obtained showed that the bacterium Lactobacillus fermentum and L. salivarius derived from quail intestine can produce antibacterial compounds that could inhibit the growth of Salmonella typhimurium, Escherichia coli (human), and E. coli (bird). Minimum concentration of the addition of CFS from L. fermentum in inhibiting the growth of tested bacteria was 30% for S. typhimurium, 30% for E. coli (human), and 20% for E. coli (bird). While the addition of CFS minimum concentration of L. salivarius in inhibiting the growth of tested bacteria was 20% for S. typhimurium, 20% for E. coli (human), and 10% for E. coli (bird). Keywords: Antibacterials, Lactic Acid Bacteria (LAB), Escherichia coli, Quail, and Salmonella typhimurium.
A Field Survey To Investigate The Flora And Fauna of Dhauladhar Nature Park in North-Western Himalayan Region of India Neeraj, Sharma Kumar
Journal of Tropical Life Science Vol 5, No 3 (2015)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.05.03.07

Abstract

Taxonomic studies on wildlife biodiversity has always been very fascinating along with the great purpose of providing valuable information on their present status concerning species number and future course of action required for their conservation. A study regarding survey of flora and fauna was carried out at Dhauladhar Nature’s Park of Gopalpur in district Kangra of Himachal Pradesh during the month of April 2015. The present study has revealed the presence of 09 mammals, 02 reptilia, 05 pheasants and 04 other birds belonging to 12 families along with 44 plant species belonging to 28 families. Further it is suggested that provisions for Himalayan snakes and butterflies, the other two imperative Himalayan fauna be created as soon as possible so that nature’s park can fulfill its  true purpose of conservation of Himalayan wild animals in real sense.
Composition of Trees Grown Surrounding Water Springs at Two Areas in Purwosari Pasuruan, East Java soejono, soejono
Journal of Tropical Life Science Vol 2, No 3 (2012)
Publisher : Journal of Tropical Life Science

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Abstract

Copper Toxicity of Four Different Aquaculture Ponds Mustapha, Moshood; Agunloye, Joshua Taiwo
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.04

Abstract

> Copper is a nutritional trace element for fish which could be deficient or toxic. Copper concentrations from four aquaculture ponds were analyzed for 12 weeks using Bicinchoninate method. Alkalinity was measured using colorimetric method with the aid of Hanna Multiparameter Bench Photometer. Temperature and pH of the ponds was measured in situ using Hanna Portable pH/EC/TDS/Temp combined waterproof tester. All measurementswere done I triplicates. Copper concentrations ranged from 0.01 to 0.10 mg/L, alkalinity ranged from 105 to 245mg/L. Weeks 1 and 12 recorded the highest and lowest Cu2+ concentrations, while lowest and highest alkalinity were obtained in weeks 1 and 12 respectively in all the ponds. Temperature and pH ranged from 29.1 to 35.9°C and 6.35 to 8.03. The trend of copper concentrations in the ponds was Natural > Earthen > Concrete > Collapsible, with concentration slightly above the normal in the ponds. This could have come from the pipes used in delivering water to the pond, copper alloy nets and mesh used in the ponds, anti-fouling agents, algaecides, water source and fish meal. Effect of elevated copper was seen in the morphology and behavior of Clarias gariepinus which include slimy mucus on the skin, aggressive and uncoordinated swimming with the opercula flared, slow growth, reduced odor perception of food and water bubbles on the ponds. Copper should be regularly bio monitored to determine toxicity in fish.

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