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INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 1,001 Documents
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Exploration of Flora Diversity and Recommending Species for Reclamation of Coal Mining with Biodiversity Concept in Besiq Bermai Forest, East Borneo Trimanto Trimanto; Siti Sofiah
Journal of Tropical Life Science Vol. 8 No. 2 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.02.02

Abstract

This research was conducted to gather basic information to support recovery coal mining area with study of flora biodiversity. Plants inventory were conducted explorative in Besiq Bermai forest. Some observations were conducted on plants as integral part of the documentation process. The result showed that there are 203 numbers of plant. They consist of 51 families of flora collection to be conserved in Purwodadi Botanic Garden. There are 53 species of orchid that collected from this forest, including of rare species and endemic orchid. There are 70 numbers of 1000 specimen floras to be collected in Nursery of coal mining to be used in reclamation program. There are 20 species of flora that be conserved because it is threatened species that based on IUCN. Ten species can be chosen to become pioneer species in reclamation of coal mining area. Biodiversity concept can be used in reclamation of post mining area. Prospective conservation area that called Arboretum is provided for in-situ conservation program. Some practical considerations are suggested for fu-ture reclamation projects.
Human and Animal Pentastomiasis in Malaysia : Review Bahaa Mohamed Abdul Latif; Azdayanti Muslim; Heo Chong Chin
Journal of Tropical Life Science Vol. 6 No. 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.12

Abstract

Pentastomiasis is a zoonotic parasitic disease induced by the larval stages of pentasomes. The disease has been reported in Africa, the Middle East and Southeast Asia and caused by the nymphs of the two genera:  Linguatula  and Armillifer and the two species L. serrata and A. armillatus regard for more than 90% of human cases. The definitive hosts of Armillifer spp. are snakes, lizards and other reptiles. The parasites live in the upper respiratory tracts and lay eggs that are passed out through respiratory secretions, saliva or faeces. Intermediate hosts are  humans, rodents and other mammals. Humans incidentally acquire the infestation by the consumption of uncooked infested snake meat or by drinking water contaminated with ova of the pentastomes. In the intestinal tract, the larvae hatch from the ova, penetrate the intestinal wall and migrate to organs in which the liver is the most common site. Human pentastomiasis was reported among aborigines in West and East Malaysia. Armillifer moniliformis was identified  in wild animals and carnivores with infection rate 1.8% and 20.7% respectively. The adults of pentostomes (A. moniliformis) were recovered from two out of six snakes Python reticulates. Recently a case of human pentastomiasis was reported in Sabah, East Malaysia, caused by nymph of Armillifer moniliformis.
The Potential of Indigenous Bacteria for Removing Cadmium from Industrial Wastewater in Lawang, East Java Agung Pambudiono; Endang Suarsini; Mohamad Amin
Journal of Tropical Life Science Vol. 8 No. 1 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.08.01.11

Abstract

Heavy metals have been used in various areas around the world especially in the industrial sector. Heavy metals contamination is very dangerous for ecosystem because of its toxicity for some organisms. Cadmium (Cd) is a dangerous metal pollutant that can cause remarkable diverse of toxic effects, in particular for humans and animals. The use of bacteria as bioremediation agents has been widely studied because more efficient, less cost, and environmentally friendly strategy. This present study aimed to isolate and identify Cd-resistant bacteria from the industrial disposal site. Wastewater samples were collected from disposal site of agar flour industry in Lawang Malang, East Java. The collected wastewater effluent was analyzed for physicochemical properties. Isolation of Cd-resistant bacteria was carried out using serial dilution. Bacterial isolates were observed and tested for their effects on the content of Cd. The content of Cd was tested daily using Atomic Absorption Spectroscopy (AAS) for seven consecutive days. Data was analyzed using one-way ANOVA (p < 0.05) and Tukey test. Characterization of potential bacterium was performed using bacterial identification kit. Four bacteria isolates have been successfully isolated from the wastewater sample. There was a statistically significant difference between groups as determined by one-way ANOVA (F = 1229.62, p = 0.00). A Tukey post hoc test revealed that all conditions are significantly different from each other. The content of Cd in wastewater sample was statistically significantly lower after taking the A isolate (3.39 mg/L, p = 0.00), B Isolate (1.47 mg/L, p = 0.00), C Isolate (1.15 mg/L, p = 0.00), and D isolate (1.95 mg/L, p = 0.00) compared to the control treatment (5.11 mg/L, p = 0.00). Two of the most potential isolates identified as Pseudomonas flourescens (C isolate) and Enterobacter agglomerans (B isolate).
Ethnobotanical Study of Plants Used by People in Hiang Indigenous Forest Kerinci, Jambi Evan Vria Andesmora; Muhadiono Muhadiono; Iwan Hilwan
Journal of Tropical Life Science Vol. 7 No. 2 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.02.02

Abstract

Indonesia is a high-abundance tropical forests country. It plays a very important role for world life because of its species richness than others. One of the forest resources in Indonesia is indigenous forests. Indonesia is rich of local wisdom such as that possessed by indigenous peoples. Indigenous peoples have a potential of the biological resources conservation. As a sustainable management tradition, communities around indigenous forest also have the efforts to preserve the forest.  The data collection of community knowledge about plants was conducted by interviews and direct-field observation. Data were analyzed using index of cultural significance. The results showed that there are 48 species of plants utilized by communities in Hiang Indigenous Forest, 27 species are used as firewoods, 15 species as building materials, 7 species as medicinal plants, 6 species as traditional handicraft ingredients and 4 species as secondary foods and traditional ritual materials. Most of widely-used plants by the community are Altingia excels, and Styrax benzoin is used as incense in a traditional ritual.
Litter Decomposition Rate of Karst Ecosystem at Gunung Cibodas, Ciampea Bogor Indonesia Sethyo Vieni Sari; Ibnul Qayim; Iwan Hilwan
Journal of Tropical Life Science Vol. 6 No. 2 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.02.08

Abstract

The study aims to know the productivity of litter and litter decomposition rate in karst ecosystem. This study was conducted on three altitude of 200 meter above sea level (masl), 250 masl and 300 masl in karst ecosystem at Gunung Cibodas, Ciampea, Bogor. Litter productivity measurement performed using litter-trap method and litter-bag method was used to know the rate of decomposition. Litter productivity measurement results showed that the highest total of litter productivity measurement results was on altitude of 200 masl (90.452 tons/ha/year) and the lowest was on altitude of 300 masl (25.440 tons/ha/year). The litter productivity of leaves (81.425 ton/ha/year) showed the highest result than twigs (16.839 ton/ha/year), as well as flowers and fruits (27.839 ton/ha/year). The rate of decomposition was influenced by rainfall. The decomposition rate and the decrease of litter dry weight on altitude of 250 masl was faster than on the altitude of 200 masl and 300 masl. The dry weight was positively correlated to the rate of decomposition. The lower of dry weight would affect the rate of decomposition become slower. The average of litter C/N ratio were ranged from 28.024%--28.716% and categorized as moderate (>25). The finding indicate that the rate of decomposition in karst ecosystem at Gunung Cibodas was slow and based on C/N ratio of litter showed the mineralization process was also slow.
Resistance of Aedes as a Vectors Potential for Dengue Hemorrhagic Fever (DHF) in Semarang City, Indonesia Martini Martini; Retno Hestiningsih; Bagoes Widjanarko; Susiana Purwantisasari
Journal of Tropical Life Science Vol. 9 No. 1 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.09.01.12

Abstract

Dengue Hemorrhagic Fever (DHF) is a significant health problem in Semarang, Indonesia. A Certain type of insecticides has been widely applied beginning in the last decade as an effort to control the mosquito vectors of dengue virus. This practice could lead to increased resistance in mosquito populations toward these types of insecticides. This research aimed to describe the resistance status of Aedes sp. populations in Semarang. A cross-sectional design study was conducted in 2015, with Aedes sp. populations sampled in several villages of Semarang. Field strains of Aedes sp. eggs were collected using ovitraps and larvae from entomological surveys. Mosquito adults were raised from the collected eggs and larvae under standard conditions and used for biochemical assays. ELISA examined Aedes sp. resistance status. The results showed that there was monooxygenase activity in Aedes sp. populations. In quantitative terms, 77.8% of mosquito samples showed an Optical Density (OD) more than the cut-off point (0.165). Resistance to synthetic pyrethroid insecticide in Aedes sp. mosquitoes population in Semarang may be caused by the mechanism of monooxygenase detoxification enzymes in particular. This case indicated that Aedes sp. has a high capacity as a vector to transmit the dengue virus in Semarang.
Micropropagation of Rubus chrysophyllus Reinw. ex Miq. and Rubus fraxinifolius Poir. Lily Ismaini; Destri Destri; Muhammad Imam Surya
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.12

Abstract

Rubus chrysophyllus and Rubus fraxinifolius are a native species in Indonesia, which has highly potential to be developed as fruits crops. Propagation is an important factor in developing a fruit cultivation. In vitro micropropagation is an important instrument to improve the quality of seedling. Our experiment was aimed to study the micropropagation of R. chrysophyllus and R. fraxinifolius. The shoot tips were cultured on MS medium supplemented with 10 mg/L indole-3-butyric acid (IBA) for enhancing roots of R. chrysophyllus and R. fraxinifolius in the in vitro condition. Then, the rooted plantlets were transplanted into cocopeat media for acclimatization. The results showed that R. chrysophyllus and R. fraxinifolius gave a different response on the medium contain 10 mg/L of IBA. The R. fraxinifolius shows the best results compared to R. chrysophyllus on 14 days after subculture. The roots length and roots numbers of R. fraxinifolius and R. chrysophyllus were 9.13 cm and 11.25; 2.20 cm and 2.00, respectively. Although R. fraxinifolius was growing better than R. chrysophyllus, but after acclimatization R. chrysophyllus were able to grow faster than R. fraxinifolius. Moreover, on the parameters of plant height shows that R. chrysophyllus (9.20 cm) were growth higher than R. fraxinifolius (4.05 cm) during acclimatization.
The Impact of Nutrition, Helminth Infection, and Lifestyle on Elementary School Student’s Achievement Ika Febianti Buntoro; Dwita Anastasia Deo; Rahel Rara Woda
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.05

Abstract

There is a lot of helminth infections and malnutrition cases in Indonesia. Some of the effects of helminth infection are anemia, diarrhea, malnutrition, intestinal obstruction, growth and developmental disorder, and also cognitive impairment. This study aimed to explore the impact of nutrition, helminth infection, and lifestyle on elementary school students’ achievement. An observational analytical study with the cross-sectional design was used. The study was participated by 65 elementary school students grade 3, 4, and 5. The study was conducted in Pasir Panjang Elementary School, Kupang, Nusa Tenggara Timur, Indonesia. The data was analyzed using chi-square. There were 7 students (10.77%) having helminth infections:4 students (57.1%) had Ascarislumbricoides infection, 2 students (28.6%) had Enterobius vermicularis infection, and 1 student (14.3%) had Strongyloides stercoralis infection. There were no differences found on students’ achievement between students with normal and low nutritional state (p = 0.917; p > 0.05) and between different lifestyle habit  (p = 0.768;  p > 0.05). However, a significant difference in students’ achievement was found between students with and without helminth infection (p = 0.036; p < 0.05). Helminth infection had a significant impact on elementary school students’ achievement, but no significant impact found for differences in nutritional state and lifestyle habit.
Effect of Vitamin D3 Supplementation to 25(OH)D, IL-17, and HbA1c Level in Pediatric Type 1 Diabetes Mellitus Rahmah Yasinta Rangkuti; Harjoedi Adji Tjahjono
Journal of Tropical Life Science Vol. 7 No. 1 (2017)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.07.01.06

Abstract

Type 1 Diabetes Mellitus (T1DM) is the consequence of autoimmune destruction process of β cells which associated with Th17 activity and low 25(OH)D level. This study was aimed to investigate the effect of vitamin D3 supplementation toward 25(OH)D level, Th17 activity (IL-17) and glycemic control (HbA1c) in pediatric T1DM. This study was designed as randomized clinical trials (RCT), double-blind, pre and post-test controlled study. Subject was children with T1DM who were divided into two groups: K1: subjects were treated with insulin 0.5–2 IU/day + vitamin D3 2000 IU/day for 3 months, K2: subjects were treated with insulin 0.5–2 IU/day + placebo for 3 months. Levels of 25(OH)D, IL-17 and HbA1c were evaluated after 3 months treatment using ELISA. After 3 months treatment, results showed that 25(OH)D level was significantly higher in K1 compared with K2 (p = 0.00), IL-17 level was significantly lower K1 compared with K2 (p= 0.022). Surprisingly, HbA1c level in K1 was not significantly different with K2 (p = 0.93). Furthermore, in vitamin D-treated group, 25(OH)D level was elevated significantly after 3 months treatment with vitamin D (p = 0.00), IL-17 level was reduced significantly after 3 months treatment with vitamin D (p= 0.001)  and HbA1c level was reduced insignificantly after 3 months treatment with vitamin D (p= 0.76). Correlation study showed that there was no correlation between 25(OH)D level with IL-17 level (p= 0.160, r= -0.284) and 25(OH)D with HbA1c (p= 0.62, r= -0.10). This study can be conclude that vitamin D3 supplementation may elevate the 25(OH)D and reduce IL-17 level but did not change HbA1c level in pediatric T1DM.
Agarose Coated Culture Plate in Tumorsphere Culture of Cervical Cancer Cell Line HeLa: an Alternative to Non Adhesive Culture Plate Putu Juniartha; Muhammad Rasjad Indra; Hidayat Sujuti; Diana Lyrawati; Tatit Nurseta
Journal of Tropical Life Science Vol. 6 No. 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.11

Abstract

Cervical cancer recurs in 90% cases and linked to cancer stem cells that able to self-renew and responsible for recurrence, metastasis, and mortality of cancer. Isolation and identification of cancer stem cells using serum-free medium needs expensive growth factors and consume time. This study try to grow tumor sphere using culture plate coated with 1% agarose as an efficient and economical alternative to non-adhesive culture plate. HeLa cell line was grew in culture plate coated with 1% agarose and non-adhesive culture plate using similar medium and culture condition. Tumor spheres morphology was observed and the colonies were counted in 7 days followed by single cell assay. Tumor spheres then counted for CD133+, CD34+, and Sox2 expression using flowcytometry. Culture plate coated with 1% agarose can be used as an economic and efficient alternative to culture tumor sphere. Using culture plate coated with 1% agarose, the tumor spheres formed in 7 days with similar morphology to non-adhesive culture plate. Tumorsphere had three dimensional – sphere shape that tightly attached, colonized, and overlapped. The tumor sphere colony counts of two plates were statistically have no significant difference (p=0,667). Single cell assay of a tumor sphere shows that it can grow new tumor spheres with similar morphology. The tumor sphere from culture plate coated with 1% agarose express CD133+ and CD34+ as much as 8.78% ± 2.14 and Sox2 as much as 35.30% ± 23.82 whereas tumor sphere from non-adhesive culture plate express CD133+ and CD34+ as much as 62.36% ± 1.06 and Sox2 as much as 98.86% ± 0.56 (p = 0000).

Page 53 of 101 | Total Record : 1001

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