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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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Struktur Populasi Ikan Tuna Mata Besar (Thunnus obesus) Dengan Analisis DNA Mikrosatelit Di Perairan Samudera Hindia Andi Bahtiar Batti; Made Pharmawati; I Gusti Ngurah Kade Mahardika
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p16

Abstract

Bigeye tuna (Thunnus obesus) is a main commodity for international markets of tuna fisheries in Indonesia, resulting in an increasing tuna capturing across Indian Ocean; Therefore, improvements in management and catchment are needed for tuna sustainability, such as a better understanding in its population structures. 30 fins tissues of bigeye tuna in total, were collected from two parts of Indian Ocean, (western Sumatra and southern Nusa Tenggara) during 20 December 2015 until Mei 2016. This study was aimed to gain some information on population structures of bigeye tuna of Indian Ocean based on microsatellite analysis. DNA analysis were conducted in a laboratory of Institute of Marine Research and Development, Gondol, Bali, Indonesia and the 1st Base -Singapore. The benefit of this study is to provide data and information on population structures of bigeye tuna of Indian Ocean. The results of Analysis of Molecular Variance (AMOVA) indicated that populations of bigeye tunas captured from the two fishing areas are included in one population.
Keberadaan Mikroba Pemfermentasi pada Minuman Kefir Air Susu Kambing Etawa Merla Pamericar; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p15

Abstract

Study about existence of fermentation microbe in kefir etawa goat milk had been studied. The objective of this study were to know total of microbe from kefir etawa goat milk. This study used descriptive method and counting population of microbe on in vitro. The variable acid in kefir measuring with pH meter. Result indicated total bacteria in kefir etawa goat milk are sebanyak 98,3 x 106 cfu/ml with total yeast 67,8 x 105 cfu/ml and pH of kefir 4.57.
PERSILANGAN INTERSPESIFIK ANGGREK HITAM (Coelogyne pandurata) DENGAN ANGGREK MUTIARA (Coelogyne asperata) Ni Wayan Deswiniyanti; Ni Kadek Dwipayani Lestari
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p15

Abstract

Black orchid (Coelogyne pandurata) and Pearl orchid (Coelogyne asperata) are endemic orchid from Kalimantan forest, shape and color suitable as ornamental flower. Many people collected the orchids from the wild for commercial purpose but not much effort on their propagation. To prevent population decreased, propagation and production of new varities via interspesific hybridisation need to be done. An important step required in plant breeding is to obtain cross compatiblity. Cross compatibility of some orchids from Indonesia are not known, including compatibility of Coelogyne pandurata x Coelogyne asperata. Information on compatibility and fertility is very important to produce good quality seed as propagation material. In this research pollination was performed on orchid plants at 2 until 5 day after flowering and pollination was done in the morning. There are three replicates for each pollination.  Pollination was done with C. pandurata (black orchid) as male parent and C. asperata (pearl orchid) as female parent. The results showed that successful pollination was obtained from pollination at 2 days after flowering. Seed capsule was harvested at 3 months after pollination but not yet perfectly ripe. Seed germination employed in vitro method on Vacin & Went (VW) and VW with modification media. Seed from C. pandurata x C. asperata abled to germinate on VW media with addition of 0,1 mg/l auxin and 0.1 mg/l cytokinin and germinated 9 weeks after planting.
Potensi Jamur Endofit pada Tumbuhan Mangrove Avicennia marina di Kuala Enok Indragiri Hilir sebagai Penghasil Antibiotika Kustiasih Lestari; Anthoni Agustien; Akmal Djamaan
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p13

Abstract

This study provides an overview of the potential of endophytic fungi isolated from leaves, stems, mangrove roots A. marina as a producer of antibiotics tested for Staphylococcus aureus, Escherichia coli, Candida albicans. This research was carried out in the microbiology laboratory of the Health Polytechnic of the Ministry of Health of Riau from March to June 2017. This study used a laboratory experimental method, the data presented in descriptive form. The stages of the study were: identification of A. marina plants, fungal isolation, qualitative tests of flavonoids isolates from endophytic fungal, and antibacterial activity test. The results showed that from 15 isolates of endophytic fungi, 5 isolates producing antibiotics. FAA 3 isolate showed the highest antibiotic activity with 24 mm the diameter of the inhibitory zone, while the lowest inhibition zone was FAA 4 isolate by forming a diameter of inhibitory zone of 7 mm and the results of qualitative tests of metabolites showed as secondary flvonoid metabolites. This research can be used as a source of information for the community in utilizing mangrove plants as an alternative in improving health.
STUDI LINGKAR TUMBUH POHON DI KAWASAN HUTAN TAMAN NASIONAL SIBERUT KEPULAUAN MENTAWAI . Mansyurdin; Tesri Maideliza; . Chairul; Ema Susiana
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p02

Abstract

Growth ring trees are formed by activity of the cambium which is influenced by the changing seasons.   In the tropical are not all of  trees species produce  the growth ring, because the season of  tropics is more uniform throughout the year and does not show sharp distinction between the periods of high rainfall and period of low rainfall. This study has concentred on several tree forest areas in Siberut National Park, Mentawai Islands. Samples were ollected by using borer on the main stem on the height of 130 cm. To see or not to see growing circle with to be checked macroscopically and microscopically. The macroscopic examination was done polished core by several grades of sandpaper in the transverse surface Tree species which have growth ring continued to microscopic observation with making slice anatomy. Based on 46 species of trees were examined, and  6 species were with found a growth ring in the number of cell mixture early wood and late wood from these species.
Potensi Minyak Atsiri Cananga odorata dan Cymbopogon citratus Dalam Menghambat Pertumbuhan Staphylococcus aureus ATCC 25923 dan Candida albicans ATCC 10231 Secara In Vitro Kadek Wegi Kurnilia; Sang Ketut Sudirga; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p07

Abstract

Cananga odorata and Cymbopogon citratus are two plant species producing inhibitory compounds in their essential oils. In our current research, the potential of their essential oils to inhibit the growth of Staphylococcus aureus and Candida albicans were investigated. MIC (Minimum Inhibitory Concentration) values of mixture of those essential oils with a ratio of 1:1 on those microbes were also assayed. Bioassays were conducted on Nutrient Agar (NA) or Potato Dextrose Agar (PDA) medium added with various ratios of the two plant species essential oils. Medium added with sterile distilled water served as control. The data obtained were analyzed quantitatively with SPSS software for Windows version 22. When significant different at p<0.05 was indicated, the analysis was continued with Duncan's multiple range test. The result showed that all treatment of essential oils applied effectively eliminated the growth of S.aureus. C.albicans appeared to be relatively resistant to 100% of C.odorata essential oil. Elimination of C.albicans was observed when the essential oil of C.odorata was combined with that of C.citratus. When compared to control, all ratios of essential oils treatments were statistically significant (p<0.05). The MIC values of essential oil mixture a ratio of 1:1 on S.aureus and C.albicans were 0,1% and 2,6% respectively. Keywords: essential oil, Cananga odorata, Cymbopogon citratus, Staphylococcus aureus, Candida albicans
Aktivitas Antimikroba Ekstrak Segar Tumbuhan Sikaduduak (Melastoma malabathricum Linn.) Nissa Arifa; Periadnadi Periadnadi
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p05

Abstract

Penelitian tentang “Aktifitas Antimikroba Ekstrak Segar Tumbuhan Sikaduduak (Melastoma malabathricum Linn.)” menggunakan Rancangan Acak Lengkap dengan pola Nested. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba ekstrak segar buah masak, daun dan bunga Sikaduduak. Hasil penelitian menunjukkan bahwa ekstrak segar daun memiliki daya hambat terbesar terhadap Staphylococcus aureus dan Escherichia coli dengan rata-rata diameter zona hambat yaitu 17,33 mm dan 14,67 mm. Terhadap Candida albicans rata-rata diameter zona hambat terbesar (11,67 mm) terdapat pada buah masak.
KECEPATAN PERTUMBUHAN KAPANG (Trichoderma harzianum Rifai A1300-F006) DAN AKTIVITAS SELULASE DALAM PENANGANANAN SAMPAH SELULOSA Pebriana Nasution; Periadnadi Periadnadi; Nurmiati Nurmiati
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p06

Abstract

Production of waste in urban areas reached 0.5 kg/person /day and 80% consisted of organic waste, one of many types of waste mostly generated is household waste. Generally, this household organic waste containing structural compoundsuch us long chains of cellulose.Therefore, the use of microorganism that can produce cellulase is very important to reduce the weight of garbage. Trichoderma harzianumis known as the most potential mold compared to other molds in converting cellulose. Sawdust and bran contain cellulose and hemicellulose that can be used as the main component in the mediafor its growth. The objectives of this study was: To find an effective ratio between sawdust and bran as growth mediafor Trichoderma harzianum, To know the cellulase activity of Trichoderma harzianum. This study has been conducted from April 2015 to Juli 2015 in the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Andalas. The results of this study concluded that an effective ratio between sawdust and bran as a growth media for Trichoderma harzianumwhich degraded organic waste was 50:50. The highest activity of cellulase in degrading organic waste is 100%.
Analisis Vegetasi dan Pendugaan Cadangan Karbon Tersimpan di Hutan Lindung Adat Ghimbo Bonca Lida Kampar Riau Dina Remina; Chairul Chairul; Nurainas Nurainas
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p04

Abstract

Indonesia is one country that has the largest tropical forests in the world with an area of 133.69 million hectares of forest area. However, the presence of Indonesian forest threatened by deforestation and forest degradation. Efforts to overcome performed with the use of forests as providers of ecological and sink carbon. Analysis study conducted to determine the composition and structure of vegetation and carbon content in indigenous Protected Forest Ghimbo Bonca Lida, to obtain data on land use biomass part terraced plot (20x100 m) combined with traffic method. Carbon As for content used data allometric equation Kettering (2001) BK = 0.11 r D2.62 . The result showed 76 species and 116 individuals belonging to 32 families. The dominant species on the level of the tree that is Syzygium cf filiformis. Carbon stocks of plant life at the top of the Indigenous Protected Forest Ghimbo Bonca Lida at 23.864,72 kg/ha and the carbon stocks nekromass of 39.071,06 kg/ha. The total carbon stocks in the Indigenous Protected Forest Ghimbo Bonca Lida of 134.529,96 kg/ha or 134,53 ton/ha
PENGARUH KOMBINASI ZAT PENGATUR TUMBUH INDOLE-3-BUTYRIC ACID (IBA) DAN 6-BENZIL AMINO PURIN (BAP) PADA KULTUR IN VITRO TUNAS AKSILAR ANGGUR (Vitis vinifera L.) VARIETAS PRABU BESTARI DAN JESTRO AG 86 Made Wahyu Cerianingsih; Ida Ayu Astarini; I Gusti Made Oka Nurjaya
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In vitro propagation study was carried out at Plant Tissue Culture Laboratory, Faculty of Agriculture, Udayana University. It was conducted using Completely Randomized Design with two factors.  The first factor was plant growth regulator (PGR), consist of nine concentration mixture of IBA (0, 0.5, 1 mg/L) and BAP (0, 1, 2 mg/L). The second factor was grape varieties, consisted of Prabu Bestari and Jestro Ag 86.  There were five replicates for each treatment combination. Variable observed included presence and absence of callus, percentage of callus growth, percentage of shoot growth, number of shoot per explants, emergence of shoots, percentage of root growth and number of roots per explants.  Data were statistically analyzed using Analysis of Variance (ANOVA).  When treatment showed a significant different (P<0.05) or highly significant (P<0.01) mean separation was conducted following Tukey Test. Data were analyzed using MINITAB Statistical Program. Results showed that PGR and varieties was highly significant for percent of callus growth.  PGR also showed significant effect on shoot growth percentage, while there was significant difference found on root growth percentage.  It can be concluded that combination of 1 mg/L IBA and 2 mg/L BAP was able to produce the highest percentage of callus growth. Addition of 2 mg/L BAP without IBA was able to produce the highest percentage of shoot growth. Percentage of root growth of Jestro Ag 86 was higher than Prabu Bestari.

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