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All Journal Jurnal Hortikultura Indonesia (JHI) METAMORFOSA Journal of Biological Sciences SIMBIOSIS Biogenesis: Jurnal Ilmiah Biologi Agrimeta: Agrimeta: Jurnal Pertanian Berbasis Keseimbangan Ekosistem WIDYA LAKSANA Jurnal Media Sains Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Paradharma: Jurnal Aplikasi IPTEK Aspirasi : Publikasi Hasil Pengabdian dan Kegiatan Masyarakat
Ni Wayan Deswiniyanti
Universitas Dhyana Pura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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Pengaruh Kolkhisin dan Gliberilin pada Periode Pembungaan Anggrek Dendrobium sp Lestari, Ni Kadek Dwipayani; Deswiniyanti, Ni Wayan
Jurnal Media Sains Vol 3, No 1 (2019): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (389.109 KB) | DOI: 10.36002/jms 3.v3i1.718

Abstract

ABSTRAKUpaya mendapatkan tanaman dan bunga yang bermutu tinggi diperlukan tehnik pengelolaan tanaman yang memadai. Kunci keberhasilan pembudidayaan dan membuat bunga tanaman anggrek dendrobium tetap indah dan cantik salah satunya ditentukan oleh teknik dan intensitas perawatan pada tahap fase pembungaan. Terdapat empat jalur regulasi pembungaan yaitu fotoperiode, otonom, vernalisasi, dan menggunakan zat pengatur tumbuh (GA/BA). Maka dari penelitian ini diharapkan mendapat konsentrasi hliberilin dan kolkhisin yang tepat untuk mempercepat periode pembungaan. Sampel diambil secara acak dengan membeli 30 bibit anggrek dendrobium dalam fase generative. Penyemprotan GA dan kolkhisin dilakukan pada saat sore hari pada pukul 15.00 Wita pada saat stomata daun terbuka. Hasil yang diperoleh Pemberian giberilin 0,1% efektif untuk mempercepat umur pembungaan, jumlah kuntum bunga, panjang tangkai bunga dan tinggi tanaman, namun tidak efektif untuk mempengaruhi ukuran luas daun anggrek Dendrobium sp. Pemberian kolkishin 0,1% efektif untuk mempengaruhi ukuran luas daun, namun tidak efektif untuk mempercepat umur pembungaan, jumlah kuntum bunga, panjang tangkai bunga dan tinggi tanaman anggrek Dendrobium sp.Kata kunci : Dendrobium sp., Gliberilin, Kolkhisin, PembungaanABSTRACTEfforts to obtain high-quality plants and flowers require adequate crop management techniques. The key of success of cultivation and to make beautiful dendrobium orchid flowers is one of them is determined by the technique and intensity of treatment at the flowering phase. There are four flowering regulatory pathways, photoperiod, autonomic, vernalization, and using growth regulators (GA / BA). Therefore, this study it is expected to get the right concentration of Giberellin (GA) and kolkhisin to accelerate the flowering period. Samples were taken randomly by 30 dendrobium orchids in the generative phase. Spraying GA and kolkhisin in the afternoon at 3:00 p.m. when the leaf stomata opens. The Result obtained by 0.1% giberelin were effective to accelerate flowering age, number of flowers, length of flower stalks and plant height, but were ineffective in influencing the size of Dendrobium Orchid leaves. It is not effective to speed up flowering age number of flowers, length of flower stalks and height of Dendrobium sp. orchid plants.Keywords: Dendrobium, Giberellin, Colchisin, flowering
KOMPATIBILITAS PERSILANGAN SELF DAN INTERSPESIFIK ANGGREK Phalaenopsis pulcherrima (Lindl.) J. J. Smith Lestari, Ni Kadek Dwipayani; Deswiniyanti, Ni Wayan
Jurnal Media Sains Vol 1, No 1 (2017): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.804 KB) | DOI: 10.36002/jms 3.v1i1.193

Abstract

ABSTRAKKompatibilitas berbagai jenis anggrek terutama jenis-jenis anggrek asal Indonesia tidak banyak diketahui. Informasi tentang kompatibilitas suatu jenis anggrek sangat penting dikuasai untuk dapat memaksimalkan perolehan biji sebagai bahan perbanyakan. Phalaenopsis pulcherima merupakan anggrek asli Indonesia, maka dapat diperkirakan bahwa jenis ini tentu memiliki keunikan tersendiri dalam hal perilaku berbunga hingga berbuah juga. Pendekatan eksperimental sangat diperlukan untuk mengetahui kompatibilitas anggrek-anggrek endemik Indonesia. Tujuan dari penelitian ini adalah untuk mengetahui kompatibilitas persentase keberhasilan persilangan secara self dan interspesifik Phalaenopsis pulcherrima. Metode yang digunakan dalam penelitian ini adalah rancangan acak lengkap (RAL) dengan menggunakan satu faktor yaitu metode persilangan dengan dua taraf yaitu persilangan self dan persilanagn interspesifik antara P.pulcherima dengan P. amabilis sebanyak 6 kali ulangan. Parameter yang diamati yaitu persentase keberhasilan silangan, umur terbentuk buah, dan panjang buah Hasil menunjukkan persilangan self P. pulcherima dengan persentase keberhasilangan silangan sebesar 0%, persilangan interspesifik sebesar 100% dengan rata- rata hari terbentuk buah 5,1 hari dan panjang buah sebesar 6,7 cm.Kata Kunci : Kompatibilitas, P. pulcherima, P. amabilis, self, interpsesifik, persilangan.ABSTRACTCompatibility of different types of orchids, especially the types of orchids from Indonesia are not widely known. Information about the compatibility of a very important species of orchids can be controlled to maximize the seed as propagation material. Phalaenopsis pulcherima is native to Indonesia, it can be estimated that this type of course is unique in terms of the behavior of flowering to fruit well. The experimental approach is needed to determine the compatibility of orchids endemic to Indonesia. The purpose of this study was to determine the compatibility of the percentage of successful interspecific crosses by self and Phalaenopsis pulcherrima. The method used in this study is completely randomized design (CRD) using a factor which is the method of crossing with two levels self and interspecific crosses between P.pulcherima and P. amabilis with as many as six repetitions. Parameters observed that the percentage of success of crosses, age formed fruit, and fruit length. Results showed self crosses P. pulcherima with cruciferous success percentage of 0%, interspecific crosses of 100% with an average of 5,1 days today formed fruit and fruit length by 6,7 cm.Key words : Compatibility, P. pulcherima, P. amabilis, self, interpsesifik, cross polination
Evaluation of antimicrobial activity and phytochemical screening of red Kamboja (Plumeria rubra L.) extracts Ni Kadek Yunita Sari; Ni Wayan Deswiniyanti; Putu Angga Wiradana
Biogenesis: Jurnal Ilmiah Biologi Vol 9 No 2 (2021)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v9i2.25409

Abstract

Natural antimicrobial sources such as red Kamboja (Plumeria rubra L.) flower extract can be utilized to treat infectious disorders caused by Staphylococcus aureus, Escherichia coli, and Candida albicans. This study aims to determine evaluate the effectiveness of methanol and ethanol extracts of P. rubra floral against E. coli, S. aureus, and C. albicans growth, as well as the amount of secondary metabolites in P. rubra extract. The study's findings indicate that the highest DIZ value of P. rubra methanol extract was 7.40 mm, 7.36 mm, and 7.30 mm for S. aureus ATCC25923 at 5%, 10%, and 20%, respectively, while the highest DIZ value for C. albicans ATCC10231 at 10%, 10%, and 20% was 25.08 mm, and 25.04 mm, respectively. The DIZ value of the P. rubra flower ethanol extract against E. coli strain was 5.26 mm at 5%, and 7.30 mm at 20%. Secondary metabolites of saponins, tannins, alkaloids, flavonoids, steroids, and phenols were present in the methanol and ethanol extracts of P. rubra flowers. In summary, our findings highlight the use of P. rubra flower extract as a biological source with antibacterial properties for the control of human infectious illnesses.
STUDI FENOLOGI PERBUNGAAN Lilium longiflorum Thunb. Ni Wayan Deswiniyanti; Ida Ayu Astarini; Ni Made Puspawati
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

Lily (Lilium longiflorum Thunb.) was known as ornamental plants, especially as cut flower. Lily varieties in Indonesia are still lacking and the price is quite expensive but demand of lily is increased steadily. The first step for successful plant breeding is understanding phenophysiology of the plants, in particular flowering phenology. The aim of this research is to provide information on flowering lily phenology, to be a basic knowledge in improving for planning plant breeding programs. In this research, plants were observed from bud initiation until fruit production (phase F0-F4). Additional morphologies observed such as: high plant, leaf shape and color, shape and type of root or bulb. Result shows that period of phase F0-F3 of sampled lily is 52 days. The plant height of lily is about 50-150 cm, has elongated single lancet leaf shape, green leaf, bulbs, and has fiberous root.
OPTIMALISASI MEDIA ORGANIK UNTUK PERBANYAKAN ANGGREK HITAM (Coelogyne pandurata Lindl.) SECARA IN VITRO Ni Kadek Dwipayani Lestari; Ni Wayan Deswiniyanti
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p13

Abstract

Populasi anggrek hitam (Coeloegyne pandurata Lindl.) terancam punah karena akibat eksploitasi yang berlebihan. Masalah tersebut dapat diatasi dengan teknik perbanyakan anggrek melalui kultur in vitro, namun harga media kultur jaringan relatif mahal. Tujuan penelitian ini adalah untuk memperoleh informasi tentang respon pertumbuhan benih anggrek hitam pada media kultur bahan organik dan modifikasinya, sehingga dapat digunakan sebagai media alternatif untuk pertumbuhan anggrek hitam yang optimal. Rancangan penelitian menggunakan Rancangan Acak Lengkap dengan 3 perlakuan yaitu media organik 1 (kentang), 2 (pisang ambon) dan 3(ubi jalar) masing-masing 10 ulangan. Data dianalisis secara statistik menggunakan Analisis Varians dan Uji Jarak Berganda Duncan pada taraf signifikansi 5%. Hasil penelitian menunjukkan bahwa media organik kentang dan pisang ambon memiliki waktu tumbuh paling cepat dan jumlah tunas paling banyak dibandingkan media organik ubi jalar. Secara statistik kontaminasi tidak dipengaruhi secara signifikan oleh media, jadi dalam hal ini media paling baik/optimum adalah kentang dan pisang ambon, yang berbeda nyata dengan ubi jalar pada waktu tumbuh dan jumlah tunas.
PERSILANGAN INTERSPESIFIK ANGGREK HITAM (Coelogyne pandurata) DENGAN ANGGREK MUTIARA (Coelogyne asperata) Ni Wayan Deswiniyanti; Ni Kadek Dwipayani Lestari
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p15

Abstract

Black orchid (Coelogyne pandurata) and Pearl orchid (Coelogyne asperata) are endemic orchid from Kalimantan forest, shape and color suitable as ornamental flower. Many people collected the orchids from the wild for commercial purpose but not much effort on their propagation. To prevent population decreased, propagation and production of new varities via interspesific hybridisation need to be done. An important step required in plant breeding is to obtain cross compatiblity. Cross compatibility of some orchids from Indonesia are not known, including compatibility of Coelogyne pandurata x Coelogyne asperata. Information on compatibility and fertility is very important to produce good quality seed as propagation material. In this research pollination was performed on orchid plants at 2 until 5 day after flowering and pollination was done in the morning. There are three replicates for each pollination.  Pollination was done with C. pandurata (black orchid) as male parent and C. asperata (pearl orchid) as female parent. The results showed that successful pollination was obtained from pollination at 2 days after flowering. Seed capsule was harvested at 3 months after pollination but not yet perfectly ripe. Seed germination employed in vitro method on Vacin & Went (VW) and VW with modification media. Seed from C. pandurata x C. asperata abled to germinate on VW media with addition of 0,1 mg/l auxin and 0.1 mg/l cytokinin and germinated 9 weeks after planting.
EFEK PEMBERIAN PAKLOBUTRAZOL PADA ANGGREK Vanda tricolor SECARA IN VITRO Ni Wayan Deswiniyanti; Ni Kadek Dwipayani Lestari
SIMBIOSIS Vol 6 No 1 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (804.434 KB) | DOI: 10.24843/JSIMBIOSIS.2018.v06.i01.p04

Abstract

Vanda tricolor Lindl. Var. Suavis forma Bali has a specific character than the forma Merapi and forma West Java that is size of flowers and fruits, spots purple in labellum purple colored labelum and floral fragrance. High genetic diversity is one of the major factors in breeding improvement. Increasing the diversity of orchid plant characteristics can be done by giving paclobutrazol. Treatment by paklobutrazol in culture medium aims to inhibit growth both in number of leaves and shoot length so that the plant becomes smaller size than their parent or original. The result of growth resistance that occurs varies based on the concentration of paclobutrazol added to medium Vacint & Went (VW) in culture of seeds V. tricolor orchid added with coconut water with concentration of paklobutrazol Control K0 (0%), K1 (1mg / l), K2 (3mg / l), K3 (5mg / L), and K4 (7mg / l). Each treatment performed 5 times repetition. The result of planting of Vanda tricolor seed explants with paclobutrazol modification in vitro was found 40% cultured imbibition and then protocorm, 8% browning on seed, and 52% contamination. This study showed descriptively the orchid seed Vanda tricolor responded to Vacint and Went (VW) media which was added with plant growth regulator of paklobutrazol, so that it was able to grow and develop until reaching phase 1 that seeds to form protocorm, but statistically the addition of plant growth regulator of paklobutrazol has not show a significant influence on the growth and growth response of orchids Vanda tricolor (P> 0.05) because the observation time is not sufficient for at least 6 months of observation and see the function of paklobutrazol is to slow the growth. Keywords :Vanda tricolor, paclobutrazol, in vitro, vacin went, protocorm
PENCEGAHAN BROWNING PADA EKSPLAN IN VITRO UNTUK PERBANYAKAN TANAMAN Lilium longiflorum Ni Kadek Dwipayani Lestari; Ni Wayan Deswiniyanti; Ida Ayu Astarini; Luh Made Arpiwi
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol 1 (2018): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

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Abstract

ABSTRAKTanaman Lilium longiflorum pada umumnya diperbanyak dengan menggunakan pemisahan umbi, namun terdapat kendala yaitu tanaman yang diperbanyak terbatas, memerlukan lahan dan tergantung kondisi cuaca dengan waktu yang relatif cukup lama. Maka, diperlukan metode baru yaitu salah satunya metode kultur in vitro karena metode ini tidak tergantung pada lahan, cuaca dan dapat memperbanyak tanaman dengan jumlah yang banyak dan cepat ,namun metode ini pun memiliki kelemahan yaitu adanya kontaminasi serta terjadi browning pada eksplan, maka diperlukan suatu metode untuk mengatasinya. Penelitian ini bertujuan mengetahui metode eliminasi browning yang efektif diantara perlakuan perendaman eksplan dalam asam askorbat dan asam sitrat. Pengamatan meliputi waktu rata-rata eksplan mengalami browning, intensitas browning seluruhnya atau sebagian dan persentase eksplan browning hingga 35 hari setelah tanam (HST). Berdasarkan hasil yang diperoleh dari perlakuan perendaman asam sitrat 500 mg/l dan asam askorbat 300 mg/l selama 30 menit dapat menurunkan waktu inisiasi browning hingga 88 % pada eksplan daun muda, 100 % pada eksplan pedicel dan 64 % pada eksplan ovarium. Menurunkan intesitas browning pada eksplan daun muda dan ovarium. Menurunkan persentase browning sebesar 80% pada eksplan daun, 0% pada eksplan pedicel dan 36% pada eksplan ovarium.Kata kunci : browning, eksplan, asam sitrat, asam askorbat, liliumABSTRACTLilium longiflorum plants are generally propagated by using bulbs, but there are some so-called limited-multiplied plants, broad conditions and depending on the conditions for a long time. So, a new method is needed, which is one of the culture methods in vitro because this method does not depend on the land, weather and can multiply plants in large and fast numbers, but this method also has weaknesses, such as mathematics and browning occurs in explants, so some things required. methods to overcome them. This study aims to eliminate the effect of effective skin color between soaking treatment in ascorbic acid and citric acid. Observations include the average time of brown, brown, or part and all explants explants to reach 35 days after planting (HST). The results obtained from soaking citric acid 500 mg / l and ascorbic acid 300 mg / l for 30 minutes can reduce browning initiation time by 88% on young leaf explants, 100% on pedicel explants and 64% on ovarian explants. Reduces browning intensity on young leaf and ovarian explants. Reducing the amount of browning by 80% on leaf explants, 0% on pedicel explants and 36% on ovarian explants.Keywords: browning, explants, citric acid, ascorbic acid, lilium
DENDROGRAM KARAKTER MORFOLOGI TANAMAN DAUN JINTEN (Coleus amboinicus L.) DENGAN GENUS LAINNYA PADA KELUARGA LAMINACEAE Ni Wayan Deswiniyanti; Ni Kadek Dwipayani Lestari; Ni Made Virginia; Kadek Eka Jaya Efendi
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol 5 (2022): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

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Abstract

ABSTRAKTanaman daun jinten (Coleus amboinicus L) merupakan tanaman yang berasal dari keluarga lamiaceae atau labiatae merupakan salah satu family atau keluarga tanaman yang memiliki manfaat sebagai tanaman obat tradisional. Beberapa genus famili tersebut di antaranya Coleus, Lavandula, Ocimum, Orthosiphon, Mentha, dan Rosmanirus. Karakter tanaman dari family ini memiliki kekhasan daun beraroma aromatik dan merupakan tanaman herba. Pada penelitian ini bertujuan untuk mendeterminasi karakter morfologi tanaman daun jinten (genus Coleus), lavender (genus Lavandula), kemangi (genus Ocimum,) Kumis kucing (genus Orthosiphon), daun mint (genus Mentha), dan daun rosemary (genus Rosmanirus), kemudian dibuat dendrogram untuk mengetahui hubungan kekerabatannya. Hasil menunjukkan Genus Coleus berkerabat dekat dengan genus Mentha dibandingkan dengan genus lainnya pada keluarga laminaceae.Kata kunci: morfologi, daun jinten, laminaceae, dendrogram
UJI AKTIFITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN LILI (Lilium longiflorum THUMB.) BERDASARKAN UMUR DAUN Ni Kadek Dwipayani Lestari; Ni Wayan Deswiniyanti; Ni Nyoman Ari Mardiati; Ni Kadek Diah Angguni
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol 3 (2020): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.707 KB)

Abstract

ABSTRAKLilium longiflorum ini merupakan tanaman yang bernilai ekonomi tinggi yang bermanfaat sebagaibunga potong, tanaman hias, bahan kosmetika dan obat. Kandungan metabolit sekunder daritanaman lili mengandung saponin, tannin, flavonoid dan senyawa antioksidan. Ekstrak tanaman lilidapat digunakan sebagai antioksidan maupun antimikroba, namun belum diketahui secara pastikandungan dan kemapuan efektifitas antioksidan. Maka dilakukan uji aktifitas antioksidan padadaun muda dan daun tua Lilium longiflorum. Metode yang digunakan yaitu metode DPPH (1,1-Diphenyl-2-picryl Hidrazil) diukur serapan pada panjang gelombang 517 nm dan besarnya aktivitasantioksidan ditandai dengan nilai Inhibition Concentracion (IC50). Ekstrak daun lilium longiflorumdibuat dalam berbagai konsentrasi dan diuji aktivitas antioksidannya. Aktivitas antioksidan (IC50)pada ekstrak daun muda lilium longiflorum 85,29 ppm dan pada daun tua sebesar 66,86 ppmsedangkan sebagai pembanding yaitu IC50 vitamin C murni sebesar 33,71 ppm. Aktivitasantioksidan ekstrak etanol daun lili tua lebih kuat dibandingkan daun lili muda, namun lebih lemahdibandingkan vitamin C. Tingkat kekuatan aktifitas antioksidan pada daun lili muda dan tuatermasuk dalam kategori kuat.Kata Kunci : Antioksidan, lili, IC50, daun, DPPH
Co-Authors A.A Ayu Permatasari Adhi, I Made Wisnu Agus Donny Susanto Bambang Hadi Kartiko Elok Faiqotus Zahro Emanuel Maramba Hamu Endah, Naftalia Sri Friningsih Helen Robinson Gerson Feoh Hasanah, Nina Huawatun I Gusti Manik Nugraha I Gusti Ngurah Manik Nugraha I Kadek Eka Jaya Efendi I Made Murna I Wayan Rosiana I Wayan Widi Karsana IDA AYU ASTARINI Kadek Eka Jaya Efendi Luh Made Arpiwi Made Dewi Sariyani Ni Kadek Diah Angguni Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Ketut Martini Ni Made Diaris Ni Made Intan Prawita Dewi Ni Made Puspawati Ni Made Virginia Ni Nyoman Ari Mardiati Ni Wayan Nursini Nyoman Ngurah Adi Sanjaya Nyoman Ngurah Adisanjaya Permatasari, Anak Agung Ayu Permatasari, Anak Agung Ayu Putri Putu Angga Wiradana Putu Chris Susanto Putu Okta Wijayanti Rai Riska Resty Wasita Sri Endah K, Natalia Wayan Nurata YUNITA HARDINI