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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 587 Documents
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Status Praesen Sapi Bali Betina pada Kebuntingan Trimester Ke-III Maureen Rosanti Hamangau; I Nyoman Suartha; I Gusti Ngurah Bagus Trilaksana
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p11

Abstract

This study aim to determine the praesen status which includes body temperature, frequency of respiration, frequency of heart rate and frequency of pulse in female bali cattle with gestational age in the third semester of pregnancy, namely gestational age of 7 months, 8 months and 9 months and to determine the effect of gestational age on the praesen status. The study was conducted at the Bali Cattle Breeding Center in Sobangan village Mengwi District Badung Regency used completely randomized design where gestational age was the group to be studied and analysis data was carried out using Analysis of Variance. The results showed that body temperature at 7, 8 and 9 months of gestation were 38,12 + 0,94; 38,16 + 0,50 dan 38,37 + 0,98. The frequency of respiration at 7, 8 and 9 months of gestation were 17,71 + 3,34; 19,05 + 3,22 and 20,87 + 4,86. The heart rate at 7, 8 and 9 months of gestation were 85,22 + 12,19 and 85,44 + 15.37 and 92,23 + 12,78. The pulse frequency at 7, 8 and 9 months of gestation were78,39 + 10,37; 81,57 + 10,73 and 88,18 + 13,01; Statistical analysis shown that there were significant differences (p<0,05) in body temperature, respiration frequency, heart rate and pulse rate at 7, 8 and 9 months of gestation. The conclusion is that gestational age affects praesen status which includes body temperature, frequency of respiration, frequency of heart rate and frequency of pulse rate in bali cattle at the third trimester of pregnancy. It is necessary to do research on the praesen status before parturition occurs.
Pertumbuhan Pembuluh Darah pada Fase Kesembuhan Luka Insisi Tikus Wistar yang Ditetesi Antibiotika Nabilah Rizky Amalia; Anak Agung Gde Jayawardhita; Samsuri Samsuri
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p12

Abstract

The mechanism of administration of antibiotic drops on incision wound healing in terms of the number of blood vessels is not yet known for its effectiveness. Therefore, this study aims to determine the efficacy of administering antibiotic drops on the number of blood vessels in the incision wound of Wistar rats (Rattus norvegicus). This study used 24 female Wistar rats with a body weight of about 150-200 grams each. Following the adaptation process in one week, an incision wound at 1.5 cm in a depth of up to the muscle (0.5 mm) is made in four groups with the control division (T0) given physiological NaCl. Treatment I (T1) drops of oxytetracycline antibiotics, treatment II (T2) drops of antibiotic amoxicillin, and treatment III (T3) drops of antibiotic cefotaxime. In each group of rats, three drops of antibiotic are given or 0.15 ml. After being treated, the skin samples of the incised mice were taken for histopathological examination to observe the number of blood vessels. The data results on the mean difference in the number of blood vessels were analyzed using Analysis of Variant (ANOVA). It continued with Duncan's Post Hoc test if there were significant differences. The results of the comparison of the mean number of blood vessels in the four treatments were 88.17 ± 77.78, 370.33 ± 277.14, 213.33 ± 41.44, and 268.17 ± 141.10. The results showed that antibiotics from the tetracycline group had the highest mean number of blood vessels compared to the other three treatments.
RESISTANCE PROFILE OF BACTERIA ISOLATED FROM LAYING HENS MANURE IN JEMBER REGENCY Enny Suswati; Elly Nurus Sakinah; Jauhar Firdaus; Abu Khoiri
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p17

Abstract

Antibiotics are becoming more prevalent as environmental pollutants, raising public health concerns. Antibiotics are routinely employed as growth promoters and therapeutic agents in poultry feeds. Because antibiotics are not completely metabolized in chicken body tissues, they are deposited in meat as parent compounds and eventually extruded into the environment via fowl droppings. Organic fertilizers based on chicken litter are typically recycled into the soil to improve the structure and fertility of agricultural land. Chicken litter, a vital source of nutrients for crop productivity, may also carry a variety of human infections that might endanger humans who consume contaminated food or water. A convenience sampling of five places was carried out. Litter samples were collected aseptically. CLSI standards were used to isolate E. coli and Proteus spp. The disc diffusion method was used to determine antibiotic susceptibility. E. coli and Proteus spp. were found in 80% and 60% of the samples. All E. coli and Proteus spp isolates derived from laying hen manure were resistant to sulfamethoxazole, ciprofloxacin, cefixime, tetracycline, amoxiclav, ceftriaxone, and chloramphenicol. However, only 50% and 33% were resistant to azithromycin. All E. coli and Proteus spp. Isolates were multidrug resistant. There was no resistance reported to cefepime or imipenem. Contamination of chicken litter with multidrug-resistant E. coli and Proteus spp. may be an underappreciated source of antimicrobial resistance (AMR) transmission to animals, people, and the environment. This demonstrates the importance of a One Health strategy to AMR surveillance and control in Jember. Monitoring AMR hazards and trends in the chicken litter would be possible with ongoing surveillance.
Efek Susu Kambing Fermentasi yang Ditambahkan Filtrat Daun Pandan Terhadap Escherichia coli Muhammad Nafis; Nurliana Nurliana; Amalia Sutriana; Sugito Sugito; Dasrul Dasrul
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p01

Abstract

Probiotics and herbs have potential as antibacterials. This study aims to determine the activity, inhibitory, and bactericidal effect of Lactobacillus casei fermented goat's milk added with fragrant pandan leaf filtrate at different concentrations to Escherichia coli. This study used a completely randomized design (CRD) type of post-test only control group design consisting of 8 (eight) treatments. Group P0 used distilled water (negative control) and group P1 used chloramphenicol (positive control). Lactbacillus casei roasted goat's milk was added with fragrant pandan filtrate at concentrations of 17.5%, 22.5%, 27.5%, and 32.5% in groups P2, P3, P4, and P5. Group P6 only used boiled milk and P7 only used fragrant pandan filtrate. Analysis of variance showed that the addition of fragrant pandan leaf filtrate to fermented goat's milk had no significant effect (P>0,05) among varied concentration groups, but had a significant effect (P<0,01) compared to the negative, positive, and comparative (fragrant pandan leaf filtrate) control groups. The results of the inhibitory and bactericidal effect showed that fermented goat's milk added with fragrant pandan leaf filtrate had the ability to inhibit and kill E. coli colonies. As conclusion, L. casei fermented goat's milk added with fragrant pandan leaf filtrate with varied concentrations has the weak antibacterial activity against E. coli
Pengembangan Vaksin Vektor Adenovirus untuk Virus African Swine Fever Berbasis Protein Non-Struktural Made Sumitha Kameswari; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p23

Abstract

African swine fever (ASF) is a viral disease of swine which causes high mortality as well as causes high economic losses in domestic pig. This study describes the process to develop an adenovirus vectors ASF vaccine with A224L and A276R coding region and its immune response in mice. Synthetic DNA containing the A224L and A276R genes was amplified and cloned into the commercial plasmid pAdenoX with an infusion cloning kit. A total of 106 AdenoX-ASF-224L-276R viruses from transfected T293 cells were injected into six mice intra-muscularly, while the other six mice were given T293 cell extract and were treated as controls. Blood without anticoagulant was collected from the facial vein of mice at 1, 3, 5, and 7 weeks after injection. Antibodies against ASF were tested by ELISA using synthetic peptides of A224L and A276R as antigens. AdenoX-ASF_A276R_A224L recombinant was successfully generated and proven. Injection of recombinant AdenoX-ASF_A276R_A224L in mice can trigger an immune response at week 7 significantly higher (p=0.00) compared to control, while weeks 1, 3 and 5 are not different from control (p>0.05). Production of recombinant adenovirus in monolayer cells and virus quantification need to be improved. Booster testing needs to be done after the third week.
Prevalensi dan Faktor Risiko Trypanosomiasis pada Kuda di Kabupaten Sumba Timur Umbu Yabu Anggung Praing; Ida Ayu Pasti Apsari; Nyoman Sadra Dharmawan
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p06

Abstract

This study aims to determine the prevalence and risk factors for trypanosomiasis in horses in East Sumba. The samples used were blood from 286 horses taken in the West and East Regions of East Sumba Regency. Blood sampling was carried out through the jugular vein. Diagnosis of trypanosomiasis is made by examining thin blood smears stained with 10% Giemsa. The risk factors for trypanosomiasis were studied by distributing specially designed questionnaires to farmers. The results showed that 2.10% of the samples were Trypanosoma evansi positive. The distribution of incidents spread to Lewa District (3.65%), Kota Waingapu District (1.72%), and Pahunga Lodu District (7.4%). The probability of trypanosomiasis infection based on the OR value is horse age more than 5 years (OR=3.185), availability/origin of drinking water (OR=2.821), method of rearing (OR=2.179), female sex (OR=1.256), location/origin of horses in the western region (OR=1.022), heavy livestock traffic (OR=0.979), and farmer's knowledge of surra (OR=0.222). Statistically these risk factors were not significantly different (P>0.05). Therefore, it is necessary to carry out active surveillance and control of trypanosomiasis in horses in East Sumba District. Further research is also needed with other diagnostic methods, such as the card agglutination test (CATT).
Profil Hematologi dan Lemak Darah Entog yang Diberi Pakan dengan Kadar Protein Berbeda Bersumber dari Keong Mas Made Rahayu Kusumadewi; I Putu Agus Kertawirawan; Ni Luh Gede Budiari
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p09

Abstract

This research was conducted to see that effect of using golden snail as alternatif fed for Muscovy ducks on different ration level to hematologi profil and blood fat. 64 Muscovy ducks of 8 weeks of age were use in this study use a randomized block design with four treatments and four replications, whereas each replicate consisted of four male Muscovy ducks. The composition of the ration used consisted of corn, rice bran, pollard, coconut meal and golden snail meal. There were four treatments included P0 (control feed), P1 (control feed + 10% golden snail meal), P2 (control feed+20% golden snail meal), P3 (control feed + 30% golden snail meal). The parameters observed were hematological profile including hemoglobin content, erythrocyte count, leukocyte count, and blood hematocrit content and than blood metabolites including cholesterol, triglycerides, HDL and LDL. The study used a completely randomized design with four treatments and three replicates. The data were subjected to analysis of variance (ANOVA). Based on the results obtained there was no significant difference in erythrocyte levels each treatment, but the hemoglobin level in muscovy duck given 30% golden snail showed significant differences in all treatments. Results of blood metabolites, showed no significant difference in triglyceride levels in all treatments, but the cholesterol content in the ration given 30% golden snail meal (P3) was lower and significantly different from other treatments. In general, giving 30% golden snail meal in the ration shows better results based on the hematological profile
Penilaian Penerapan Biosekuriti Pasca Wabah African Swine Fever pada Peternakan Babi di Kabupaten Kupang Nusa Tenggara Timur Galih Shinta Kurniawati; Annytha Ina Rohi Detha; Novalino Harold Geoffrey Kallau
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p18

Abstract

African swine fever (ASF) is an infectious and hemorrhagic disease that attacks pigs caused by a double-stranded DNA virus, in family Asfarviridae and genus Asfivirus. Known that vaccines and treatments for pigs infected by the virus have not been found can increase the risk of transmission in pig farms, so that biosecurity is a very important measure in reducing or minimizing the spread and prevention of the disease. The purpose of this study is to identify the types and evaluation of biosecurity application techniques on pig farms after ASF outbreak in Kupang Regency. Method of this study is used cross sectional method and descriptive analysis by conducting observational interview and data collections by filling the questionnaires. Data of this study are presented in the form of a weight processing table and a comparison table of types of biosecurity. There are three types of biosecurity, conceptual, structural and operational biosecurity. Value of the application of biosecurity of the three types of biosecurity in six farms got a good category, namely conceptual biosecurity has value of 128, structural biosecurity has value of 261, and operational biosecurity has value of 204. Evaluation the values of the three types of biosecurity in 6 pig farms has good category. This category is evidenced by the application of the three types of biosecurity by the six pig farms. Researchers suggest to increasing the application of biosecurity, especially manage the distances from residential areas by means of higher walls, making septic tanks for waste collection so that they are not environmentally friendly, providing sanitation and disinfection facilities, namely places and soap for washing hands and feet, toilet facilities, and procurement of PPE for workers.
Gambaran Histopatologi Pulmo Ayam Broiler yang Diberi Infusa Daun Dadap dan Mengalami Stres Pengangkutan Ryan Helmi Habibi; Ida Bagus Oka Winaya; I Made Merdana
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p02

Abstract

To get to the hands of consumers, it is necessary to transport broiler chickens from the warehouse to the marketing place. The transportation process often causes stress to broiler chickens, this is due to the long distance traveled when transportation is inadequate. This situation causes changes in organs, one of which is the lungs. Due to the potential of Dadap leaf as an antistress, this study aims to determine the effect of Dadap leaf infusion (Erythrina subumbrans) on pulmonary histopathology (Pulmonary ventilation) of broiler chickens experiencing transportation stress. A total of 30 male broiler chickens aged 4 weeks were taken as samples in this study. This study used a completely randomized design (CRD) with a factorial 3×2×5 pattern with three replications and the number of samples used were 30 broiler chickens which were divided into 5 treatment groups, namely negative control (P0), vitamin C 2 grams/L (P1), 1000 ppm Dadap leaf infusion (P2), 2000 ppm Dadap leaf infusion (P3) and 3000 ppm Dadap leaf infusion (P4). The data obtained were analyzed using the Kruskal-Wallis test. If there is a difference, it is continued with the Mann-Whitney test. performed using a scoring method on five visual fields, with a score of one meaning mild/focal, two to three meaning moderate/multifocal, more than three meaning severe/diffusion. The results showed that the mean of pulmonary histopathological damage with accurate variables was significantly different. The data obtained were the highest damage occurred in the P0 group (negative control) and the lowest damage occurred in the P3 group (3000 ppm dadap leaf infusion). The conclusion of this study is that 10% Dadap leaf infusion can reduce pulmonary damage and can be used as an antioxidant.
Enteritis dan Infeksi Sekunder Coccidiosis pada Ayam Broiler di Kota Tegal, Jawa Tengah Refina Sastya Qomariyuti; Rully Abiyoga Majid; Nafisa Raihana Amany; Rizni Fania Fajrin; Tyagita Tyagita; Muhammad Viqih
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p22

Abstract

Excessive chlorination in chicken drinking water can trigger pathogenic microbial infection, such as Eimeria protozoa infection which causes coccidiosis. This disease can cause losses in farmers because it causes poor weight gain and high mortality. In this study, it was known that broiler chicken on farm in Tegal were reported with inappetence, lethargy, weakness, paleness and bloody diarrhea. Water examination was performed and found 600 ppm chlorine in the drinking water and the litter thickness was less than 5 cm. Post mortem examination found enlargement of the heart and kidney, caecal tonsil and caecal bleeding, swelling of the intestine and enteritis. Based on that pathological anatomy lesion and examination of oocysts per gram of feces, the chicken diagnosis as enteritis followed by coccidiosis as a secondary infection due to Eimeria tenella with a severe infection. Therefore, control can be carried out by giving Capzuril® or Agricox®, vitamin K, vitamin A and probiotics and prevention by controlling the chlorination of the drinking water and increasing the thickness of the litter.

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