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Buletin Veteriner Udayana
Published by Universitas Udayana
ISSN : 20852495     EISSN : 24772712     DOI : https://doi.org/10.24843/bulvet.
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology Public Health Veterinary
The Buletin Veteriner is focused on Veterinary Medicine and Animal Sciences study with its various developments
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 587 Documents
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Efikasi Afoxolaner terhadap Demodekosis General pada Anjing Berdasarkan Histopatologi Kulit Jeremy Christian; Putu Devi Jayanti; Sri Kayati Widyastuti
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p07

Abstract

Demodicosis is the most common skin disease found in dogs, caused by the proliferation of Demodex sp. mites. Based on the distribution of the lesions, demodicosis is divided into local and generalized. This study aims to determine the efficacy of Afoxolaner against General Demodicosis in dogs. A 1.5 year old mixed-breed male dog weighing 7.8 kg came to the Internal Laboratory, Faculty of Veterinary Medicine, Udayana University, with anorexia, weakness, and hair loss in the facial area, around the ears, neck, limbs, sides, back and extremities. On physical examination found alopecia, erythema all over the body, crusts, pustules in the medial caudal limb. Dogs exhibit high levels of pruritus. Routine haematological examination showed that the dog had anemia and leucocytosis. Diagnosis based on microscopic examination of deep skin scraping is Demodicosis. The prognosis in this case is fausta. Case dogs were treated with Afoxolaner (NexGard®), Azithromycin Dihydrate (10mg/kgBB, PO, q24h) and Chlorpheniramine maleate (4mg/day, PO, q12h) for a week. Histopathological examination of the skin before drug administration revealed pieces of demodex in the hair follicles, furunculosis, inflammatory cell infiltration of the stratum spinosum and dermis layer, necrosis accompanied by haemorrhagic exudate, follicular dilatation, and peri folliculitis. Post-therapy, the results of histopathological examination showed good results, with no pieces of mites found, the size of the follicle returned to normal, and no inflammatory cell infiltration was seen in the peri follicle. On the 10th day it was seen that the dog's condition had improved, the results of the skin scraping showed a reduced number of mites in one field of view. Afoxolaner should be given once a month for three months to eliminate and prevent mite infection in dogs.
Respon Pemberian Imunoglobulin Yolk Anti-Canine Parvovirus pada Anak Anjing yang Dipelihara di Area Terkontaminasi Canine Parvovirus Angel Novelyn Leonard; I Gusti Ayu Agung Suartini; Iwan Harjono Utama
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p16

Abstract

Canine parvovirus is an infectious virus that mostly affects puppies. So far, prevention and therapy of CPV infection in dogs has been limited to providing supportive therapy. Prevention of CPV infection is very important, especially in puppies whose parents have not been vaccinated. The purpose of this study was to determine the response of puppies given egg yolk containing IgY anti-CPV dose of 3 ml/head/day and kept in a contaminated location. One possible modality is the administration of passive antibodies in the form of egg yolk containing antibodies (Immunoglobulins Yolk/IgY) that are specific for CPV. This study used 5 puppies aged 2 months divided into two groups, treatment group (P) which was given 3 ml/head/day of anti-CPV IgY with a dose of 214 HI units and 2 control groups. For 14 days the dogs were observed for clinical symptoms, serum was tested by the HI method to determine the presence of antibodies to CPV and leukocyte profile was detected by blood smear and examined manually under a microscope. The results showed that the maternal antibody titer was below the protective titer. Provision of egg yolk containing anti-CPV IgY was able to maintain protective antibody titers up to day 12 of treatment. Of the 3 puppies who were given egg yolks containing IgY, two of them died because they were positive for CPV. One recovered after Intravenous IgY anti-CPV therapy. It was concluded that the administration of anti-CPV IgY egg yolk at a dose of 3 ml/puppy/day was not protective enough to protect puppies from CPV infection. Effectivity can be increased by increasing the dose given.
Dermal Carcinoma pada Anjing di Zoo Klinik Makassar Nurul Sulfi Andini; Wa Ode Santa Monica; Sri Deniaty; Wahyuni Wahyuni; I Wayan Nico Fajar Gunawan
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p19

Abstract

Dermal carcinoma or skin cancer in dog is a skin cancer consisting of various types of cancer which are included in it. The tumor appears as a solitary, non-encapsulated lump, generally hairless, and occasionally ulcerated, which moves freely. The diagnosis is generally made through microscopic examination of a sample of tumor cells (biopsy). Dermal carcinoma cases are cases that often occur in pets but reports of these cases are still rare especially in Indonesia. The purpose of writing is to describe clinical manifestations, clinical course, and case prognosis. This case report was obtained from the Zoo Clinic Makassar. A dog "Pororo" came with the owner with a temperature of 38.40 C, had an enlarged mass in the medial part of the radius dexter, encapsulated, with an estimated diameter of about 6 cm. Physical examination was carried out, followed by laboratory examination found that the WBC level was very high and anemia. Handling of cases in the form of removal of the tumor through surgery and followed by histopathological examination with Haematoxylin Eosin staining to determine tumor development stage, degree malignancy, prognosis and therapy. The results of histopathological examination stated that the cells found were not uniform and lead to metastasis. The diagnosis of Pororo disease is carcinoma dermatitis but the type is not clear because the primary cells have not been found. The prognosis is infausta, the presence of metastases worsens the prognosis of cases, and it is possible that new tumors will reappear after removal. Handling for similar cases should be done quickly and precisely so that the case prognosis can be better.
Pola Warna Sapi Taro Yolla Noviolita; Ni Nyoman Werdi Susari; Sri Kayati Widyastuti; I Ketut Suatha; Luh Gde Sri Surya Heryani
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p03

Abstract

Taro cattle are a group of cattle that are uniquely different from Balinese cattle with a relatively small population and reared in Taro Village, Tegallalang District, Gianyar Regency, Bali and are sanctified and used in religious ceremonies. Taro cattle are characterized by white hair, skin, iris, hooves, and horns. The difference in hair/skin color of taro cattle is due to the influence of melanin pigment. This study is an observational study using purposive sampling technique where 52 male and female taro cattle skin observations were made at Yayasan Lembu Putih with the aim of providing information and scientific evidence as well as guidelines and references for further research. The data obtained were then analyzed using relative frequency (percentage). The results showed that there are other colors found. There are six colors of taro cattle, putih kemerahan color with a total of 40.39%, in females as much as 32.69% and males 7.69%; putih gading color with a total of 38.46%, in females 17.31% and males 21.15%; putih color with a total of 1.92% which is only found in females; sudamala and cemeng colors are only found in the male population 7.69% and 3.85% respectively; and coklat color is only found in the female population as much as 7.69%. Improvements are needed in breeding and mating management, recording systems and checking taro cattle regularly every year.
Pemberian Bee Pollen Meningkatkan Jumlah Spermatosit Primer dan Memperbaiki Kualitas Tubulus Seminiferus pada Tikus Jantan yang Terpapar Asap Rokok Febe Adonia Renandra Hermawan; Desak Nyoman Dewi Indira Laksmi; Wayan Bebas
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p14

Abstract

This study aims to determine the effect of bee pollen on increasing the number of primary spermatocytes and the quality of seminiferous tubules in white rats exposed to cigarette smoke. This research is a study that uses a RAL research design (Completely Randomized Design). The samples in this study were male white rats aged 3-4 months with a body weight range of 200 grams, as many as 18 rats were divided into three groups, namely 6 rats in the control group, 6 rats in the P1 group, and 6 rats in the P2 group. P0 = the group without bee pollen was exposed to cigarette smoke, P1 = the group given one dose of bee pollen was exposed to cigarette smoke, and P2 = the group with two doses of bee pollen was exposed to cigarette smoke. All rats were given an adaptation period of one week, then treated for 42 days. On day 43, the rats will be necropsied and the histology of the seminiferous tubules will be seen. The results showed that there was a significant increase in the number of primary spermatocytes and the quality of the seminiferous tubules on bee pollen administration. From the results of this study, it can be concluded that the administration of bee pollen can increase the number of primary spermatocytes and improve the quality of the seminiferous tubules in rats exposed to cigarette smoke. From the results of this study, it is necessary to conduct further research to measure the levels of free radicals in the testicular tissue of white rats exposed to cigarette smoke in their reproductive system.
Karakterisasi Virus Avian Influenza H5N1 Asal Peternakan Ayam Petelur Kecamatan Legok, Kabupaten Serang, Banten Nurfildza Wafeta Abharina; Gusti Ayu Yiniati Kencana; I Nengah Wandia
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p24

Abstract

Avian Influenza virus infection is still a serious concern in the poultry sector in Indonesia. The molecular character of the AI virus that easily mutates is thought to be a factor in the AI virus continuing to spread and having endemic status. This study aims to characterize the Avian Influenza virus from laying poultry in Legok District, Serang Districk, Banten as a form of preparation for the AI H5N1 vaccine. The test sample used is a collection of isolates owned by PT. Sanbio Laboratories, Bogor with the name Legok isolate. This study used two comparator isolates, namely Sukoharjo isolate (A/Duck/Sukoharjo/BBVW-1428/2012) and Garut isolate (A/Chicken/Garut/BBVW-223/2007) which are the master seeds of the local AI H5N1 vaccine. Virus isolation was carried out on embryonated chicken eggs (TAB) aged 9 days and then the virus isolation results were tested using the fast and slow hemagglutination (HA) test to detect the presence of the virus, the Reverse Transcritase Polymerase Chain Reaction (RT-PCR) test was used to see AI virus subtypes and then confirmed by sequencing tests to see the genetic distance and kinship between the isolates tested. The result is that Legok isolate is in the same clade as Sukoharjo isolate, which is in clade 2.3.2, but Legok isolate forms a new subclade 2.3.2 variant, while Garut isolate is in clade 2.1.3. The genetic distance for each isolate obtained was that Legok isolate to Sukoharjo isolate had a genetic difference of 3%, homology 97%, Legok isolate to Garut isolate had a genetic difference of 11.5%, 88.5% homology while Sukoharjo isolate to Garut isolate the genetic difference is 9% and the homology is 91%. The conclusion is that the Legok isolate has different characteristics from the Sukoharjo isolate and the Garut isolate. This indicates that the Legok isolate has a mutation, so it is necessary to carry out monitoring and further research for vaccine seeds from Legok isolate.
Identifikasi Molekuler Blastocystis sp. pada Monyet Ekor panjang di Taman Nasional Alas Purwo Edward Yonas Kristijanto; Wiwik Misaco Yuniarti; Mufasirin Mufasirin; Poedji Hastutiek; Endang Suprihati; Boedi Setiawan; Puput Ade Wahyuningtyas; Doohan Mahendra
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p05

Abstract

Zoonotic diseases can be transmitted through close interactions between long-tailed monkeys. Blastocystis sp. is one of the parasites that can attack mammals and is most commonly found in the intestinal tract. This study aims to analyze the presence of infection and the phylogenetic tree of Blastocystis sp. in long-tailed monkeys in Alas Purwo National Park, Banyuwangi, East Java. Identification of Blastocystis sp. in this study using morphological and molecular methods. A total of 100 stools were examined microscopically using the floating method, showing that 61 samples were positive, followed by a PCR test with a target of 600bp. PCR results obtained three positive samples followed by squencing. Sequences processed in BLAST isolate samples TNAP2 and TNAP9 having homology with Blastocystis sp. Subtype 3 was 98-99%, while the TNAP7 isolate had a lower homology level of 78-79% and the level of phylogenetic analysis of the TNAP2 and TNAP9 isolates was related to Blastocystis sp. from the Philippines (KY610153.1) and Egypt (OP942294.1) and the TNAP7 isolate is related to Blastocystis sp. from Thailand (MH197670.1, MH197668.1). Isolate Blastocystis sp. from Alas Purwo National Park has high homology analysis results to Blastocystis type hominis from Rep. Czech and Chinese by 80-99%, it is possible to have a connection with the zoonotic problem.
Efisiensi Penggunaan Enzim Taq Polymerase pada Pengujian Polymerase Chain Reaction Luh Dewi Anggreni; Ni Made Ritha Krisna Dewi; Ni Putu Sutrisna Dewi; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p10

Abstract

Polymerase Chain Reaction (PCR) is a very sensitive diagnostic test. The Taq Polymerase enzyme is one of the most important components in the Polymerase Chain Reaction (PCR) assay. The taq polymerase enzyme is the most expensive component. The use of taq polymerase in the PCR test is less efficient. To determine the efficiency of using the Taq Polymerase enzyme and at what smallest volume it can still be used in the PCR test method, a study was conducted by making the PCR volume smaller (5µl, 10µl, and 25µl) than the standard (50 µl). Three isolates of nasal and anal swabs of dogs infected with Parvovirus were extracted using the DNA Purification Kit. The extraction results are used as DNA templates for PCR testing. Amplification using the PCR Apply Biosystem Thermal Cycler machine. Visualization of amplification products using 1% agarose gel electrophoresis. The test results show that the PCR volume is smaller than the standard and is able to visualize the PCR product well. In 5µl volume PCR, not all samples were amplified well. Samples 1 and 3 show a thick band at the same position as the positive control. While sample 2 does not show any bands. At the PCR volume (10µl, 25 l and 50µl) all samples were amplified well. Samples 1 and 3 show a thick band at the same position as the positive control. Amplification of samples 1 and 3, the gene has a nucleotide length of 910bp (according to oligonucleotides), while sample 2 also shows the presence of a band but its position is lower and thinner than samples 1, 3 and positive control. The difference in the thickness of the amplified band was caused by differences in the concentration of the extracted DNA. The difference in amplified band height in sample 2 means that the amplified gene is not a parvo-specific gene. The conclusion of this study is that the volume of PCR testing with a volume that is smaller than the standard is able to visualize the parvo virus DNA gene well. To efficiently use the taq polymerase enzyme in PCR testing, a PCR volume of 10 l can be recommended as a PCR testing protocol for Parvo Virus DNA.
Analisis Limbah Sapi yang Berpotensi Mencemari Lingkungan dan Menularkan Penyakit pada Masyarakat I Ketut Suada; I Wayan Masa Tenaya
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p38

Abstract

Cattle farm waste is a source of environmental pollution and a source of disease transmission in society. The emergence of environmental pollution from a farm is mostly caused by livestock waste not being handled properly. The aims of this study were to analyze pollutant elements and the potential for the spread of disease to the community. This study used observational and purposive sampling methods, namely cattle ranch waste samples taken from four traditional cattle groups in South Gianyar. A total of one liter of samples was collected from each group and brought to the Veterinary Public Health Laboratory, Udayana University for analysis. The content of Total Suspended Solid (TSS), Ammonia, Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Coliform and E. coli were analyzed using the methods of Gravimetric, Spectrophotometer, Winkler, Titration-spectrophotometer, and Most probable number (MPN) respectively. The results showed that the value of TSS were 26.75 mg/L, ammonia 7.86 mg/L, BOD5 171.817 mg/L, COD 605.675 mg/L, pH 8.075, Coliform 22 million/100 ml, and E. coli was 20 million/100ml. In conclusion, the cattle farm waste in the current study had the potential to cause environmental pollution and infectious disease to society, contained exceeded the quality standards parameters for BOD5, COD, Coliform, and E. Coli and the bacteria have a potential to cause infectious disease in the community. It was recommended to manage and treat the cattle waste properly based on the recommended breeding practice, to minimize its impact on the environment and public health.
Efek Neem Gum Terhadap Kadar Glukosa Darah Tikus yang Diinduksi Streptozotosin Ichlasul Mahdi Fardhani; Jauhar Firdaus; Zahrah Febianti; Hairrudin Hairrudin; Cholis Abrori; Elly Nurus Sakinah
Buletin Veteriner Udayana Vol. 15 No. 5 October 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i05.p35

Abstract

Pharmacological therapy for diabetes, which is primarily made up of chemicals, still causes a lot of side effects. The numerous side effects of pharmacological therapy for diabetes have generated innovations in alternative diabetes therapies using ingredients derived from nature. One of the natural ingredients with an anti-hyperglycemia effect is neem gum (NG), or the sap of the neem plant (Azadirachta indica). The aim of this study was to determine the effect of NG on the reduction of blood glucose levels. Moreover, this study had six groups, including a normal control group and groups with doses of 0, 3.75, 7.5, 15, and 30 grams/kgBW. The results showed that NG reduced blood glucose levels in diabetic rats. The paired t-test showed a significant reduction in blood glucose in all groups administered NG except for the dose 3.75 group (P<0.05). The minimum and maximum effective doses of NG were calculated using the quadratic regression test with the equation y = 1.059x2–46.576x+408. With a target blood glucose level of between 80 and 126 mg/dL, the minimum and maximum effective doses of NG were obtained in the range of 12–15 mg/dL. This study concluded that administering NG to diabetic rats can reduce blood glucose levels. Phytochemical studies and research are needed with serial examinations using the effective dose range of neem gum.

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