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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 12 Documents

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Search results for , issue " Vol 18, No 1 (2019)" : 12 Documents clear

ESTIMASI HERITABILITAS DAN RESPONS SELEKSI IKAN NILA HITAM (Oreochromis niloticus) DI TAMBAK Robisalmi, Adam
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3540

Genetic improvement in the establishment of a rapid growth strain of Nile tilapia might be achieved through selective breeding program This research was aimed to estimate heritability and response selection of nile tilapia in brackishwater pond. Breeding activity was carried out in fresh water in hapa 2x1x1 m. Males and females were breeding in pairs with a ratio of 1: 1. The number of established families was 19 families of 50 broodstock pairs. Harvesting of larvae was done on day 14. Larvae were collected and then kept in net 2 x 2 x1 m with stocking density 125 fish/m2 for 60 days. The next stage was sexing by grouping the male and female populations in each family. Grow out was carried out in net cage (3 x 5 x1.5 m ) that were placed in brackiswater pond at salinity 25–30 g/L with density 10 fish/m2. Selection of broodstock in the male and female populations was done on weight of 250–300 g. Cut off selection was determined on weight from 316 to 382g (male) and 221–315 g (female). The results of this study showed that the estimation value for heritability of male and female were 0.42 ± 0.22 and 0.42 ± 0.23 and the estimation value for response selection were 10.66% (male) and 10.92% (female).

PARAMETER POPULASI IKAN BETOK (Anabas testudineus (BLOCH, 1792)) DI EKOSISTEM PAPARAN BANJIR SUNGAI MUSI, Lubuk Lampam Nurdawati, Syarifah; Fahmi, Zulkarnaen; Supriyadi, Freddy
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3107

Anabas testudineus Bloch, 1792 is economically important fish species found in Lubuk Lampam floodplains of Musi River. Fishing activities that take place throughout the year can affect reproduction patterns and recruitment (new additions) of fish. This study aims to evaluate several parameters of betok fish populations that can be used as input for fish resource management policies in floodplains of Musi river. A total of 3271 samples of betok fish caught by fishermen from March to November 2011 were collected to obtain data on fish length and weight. Growth parameters (L∞, K, Z dan M) were calculated using FiSAT II based on the length frequency distribution of fish. The values of L∞ and K were obtained at 204.75 mm and 0.24 / year respectively while the maximum age of fish was estimated to be 4.36 years. The total mortality (Z) and natural mortality (M) of betok fish is 0.99 / year and 0.42 / year, Fishing mortality (0.56/year). The exploitation rate (0.57) was slightly lower than the predicted value (Emax= 0.69) indicating that Anabas testudineus was near fully exploited in the Lubuk Lampam floodplain ecosystem.

PENINGKATAN SINTASAN LARVA IKAN KERAPU SUNU (Plectropomus leopardus) MELALUI MANAJEMEN PEMELIHARAAN YANG SESUAI Kusumawati, Daniar; Asih, Yasmina Nirmala; Seti, Ketut Maha
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Survival rate of coral trout seed produced from aquaculture was relatively low. In 2015, high mortality was found at the early stage of larval development (<D10), approximately 64.37 % of the total production. There has been no standard management for coral trout (Plectropomus leopardus) larval rearing, which was used to adopt management for grouper (Epinephelus spp.) larval rearing. This study was to apply suitable management for coral trout larval rearing into aimed to decrase mortality at early stage larval development and to increase survival rate. There were two different managements in this study: A. applying the results of previous research of coral trout larval rearing from 2004 to 2015, and B. using standard management for grouper larval rearing. The results showed that management A was more suitable management for coral trout larval rearing compared to management B. Management A resulted in the increasing of survival rate by five times (p=0.0056) and of growth rate at early stage by 1.15 times (p=0.2338) higher than those of management B.

HUBUNGAN SEBARAN MAMALIA KECIL DENGAN KONDISI LINGKUNGAN DI HULU DAS CITANDUY, JAWA BARAT Maharadatunkamsi, Maharadatunkamsi
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Citanduy watershed is one of major watersheds in Java that located through north to south covering four regencies, these are: Ciamis, Tasikmalaya, Garut and Cilacap. As a consequences of environmental changes, this watershed that covering an are about 473.967 ha has faced in deterioration on its roles. This research was aimed to study on the distribution of small mammals at the upper of Citanduy watershed according to their habitat and altitudinal changes. Results indicated that 20 species of small mammals clustered in three groups of habitat. Group 1 consisted of small mammals that occupied the habitat of mixed pines-calliandra. Group 2 comprised of small mammals lived at secondary forest, bambu plantation, and mixed secondary forest-bamboo; and group 3 were mainly occupied shrub. In addtion, an altitude was also affected the distribution of small mammals in the area surveyed. The results of this study are to be used as a basic information that describe the quality of upper Citanduy watershed.

VARIASI INTERSPESIFIK JULANG (AVES: BUCEROTIDAE) INDONESIA BERDASARKAN GEN CYTOCHROME-B DNA MITOKONDRIA Jarulis, Jarulis; Solihin, Dedy Duryadi; Mardiastuti, Ani; Prasetyo, Lilik Budi
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.3714

Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia.

PATOGENISITAS CENDAWAN Lecanicillium sp. PTN01 TERHADAP PENGGEREK TONGKOL JAGUNG Helicoverpa armigera (HUBNER) (LEPIDOPTERA: NOCTUIDAE) Ginting, Sempurna; Santoso, Teguh; Munara, Yayi; Anwar, Ruly; Sudirman, Lisdar
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Helicoverpa armigera is one of the most important agricultural pests because it has a high reproductive rate and resulted in economic losses. One of the H. armigera control techniques that is in accord with IPM principle is the utilizing of entomopathogenic fungus. The aim of this research was to test the pathogenicity of Lecanicillium (Lecanicillium sp. PTN01) against H. armigera. Pathogenicity test was conducted by conidia application of Lecanicillium sp. PTN01 on both the larvae and eggs at density of 105, 106, 107 conidia/ml. The control group was only treated with steril water. Polymerase chain reaction (PCR) was performed for molecular identification Lecanicillium sp. PTN01. The results showed that Lecanicillium sp. PTN01 able to inhibit egg hatching 13.75%, and cause mortality of first instar larval survival 98.75%. The result of virulence test to the highest larval mortality at 107 conidia/ml density was 41,25%, with the values of LT25, 50, 75 were 3.95, 7.12, 12.82 (days) and LC25, 50, 75 were 4.6 x 105, 1.7 x 106, 4.6 x 109 (conidia/ml). The DNA sequence analysis of ITS 1 and ITS 4 primers showed that Lecanicillium sp. PTN01 was similar to fungus species L. kalimantanense strain BTCC F23 with 94% homology.

SELEKSI BERBANTUKAN MARKA MOLEKULER UNTUK PEMBENTUKAN CALON VARIETAS PADI TURUNAN ESENSIAL SITU PATENGGANG Yuriyah, Siti; Utami, Dwinita Wikan; Nurani, Siti; Nasution, Anggiani; Lestari, Puji; Dadang, Ahmad; Suwarno, Suwarno
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The utilization of molecular marker technology for Blast (Pyricularia grisea) resistance in rice breeding could accelerate and improve the precision of selection. This study aimed to identify and to select the BC3F2 progenies from Situ Patenggang and the opted Blast Monogenic Lines based on their resistance to blast disease in green house and field using molecular characterization. A total of 200-300 BC3F2 population strains resulted in 4 crosses between Situ Patenggang varieties and Monogenic Varieties IRBLta2-Re, IRBLkp-k60, IRBLi-F5 and IRBLa-A were used as total genetic material in the study. Blast resistance assay was initially carried out in a greenhouse and further selection was conducted in the endemic blast area, Sukabumi. The selected Molecular marker was STS (Sequence Taq Sites) marker for foreground selection and 384-SNPs (Single Nucleotide Polymorphism) chip for background selection. The number of lines from each cross was selected by greenhouse assay i.e: 25 lines derived from Situ Patenggang/IRBLta and Situ Patenggang/IRBLkp, 21 plants for crossing Situ Patenggang/IRBLi, and 22 plants for Situ Patenggang/IRBLa-A. The results of field experiment revealed that blast resistance response of the selected lines was varied from 0 to 5. Lines of foreground were successfully selected using STS markers specified for Pii, Pita, Pikp and Pia genes. As for the background selection by SNPs markers, some lines carried the recurrent parent genetic background, Situ Patenggang. Overall, 20 resistance lines that harbored the target genes and Situ Patenggang background were obtained. Further observation was entailed to these twenty selected lines in order to attain promising lines candidate for blast resistance.

STRUKTUR TULANG DAN OTOT SIRIP KAUDAL KOMPLEKS Andamia heteroptera Bleeker (IKAN AMFIBI) Nugroho Susanto, Gatot
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i1.2208

Andamia heteroptera, known as rockskipper fish, is one of amphibious fish. Most of amphibious fish use caudal fin to facilitate locomotion in land and water. The modification is fusion of os hypural and reduction-fusion os epural and uroneurals. The aim of this study was to acces skeleton and musculature structure of A. heteroptera caudal fin. Alizarin red-Alcian blue staining revealed that caudal fin of A. heteroptera has wider spina hemalis and spina neuralis support the locomotion. There is modification of caudal fin structure for support the locomotion. Caudal fin musculature of A. heteroptera is identified as primitive type, since its lack of musculus hypochordal longitudinalis.

KONDISI PLANKTON PADA TAMBAK UDANG WINDU (Penaeus monodon FABRICIUS) DENGAN SUBSTRAT BERBEDA Hendrajat, Erfan Andi; Sahrijanna, Andi
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Plankton is one of water microorganisms that plays an important role as natural food for fish and shrimp and as an stability indicator of water or aquatic environment.The purpose of this study was to determine the composition and abundance of plankton in tiger shrimp ponds using concrete ponds without substrate, concrete ponds using sand substrate and ponds. Sampling of plankton and water quality was carried out from March to July 2016 at PunagaTakalar Experimental Pond at 6 ponds consisted of concrete ponds without 2 subplot of sand (pond A) and concrete pond with 2 subplate sand subplate (pond B) respectively measuring 1,000 m2 and a 2-square-meter plot of 2,500–3,000 m2 (pond C). The results showed that plankton abundance in pond A ranged from 37–349 individuals/L, pond B ranged from 35–1,399 individuals/L and pond C ranged from 54–999 individuals/L. The most common phytoplankton genera in this study was Oscillatoria while for zooplankton is of Acartia. The diversity of plankton in ponds A, B and C is included in the community of unstable to moderate biota. In general, the diversity of plankton in pond B is included in the community of moderate biota. The plankton uniformity index in pond A, pond B and pond C is generally close to 1 this indicates that the existence of plankton species in the three ponds is relatively even.

THE MACROFUNGI DIVERSITY AND THEIR POTENTIAL UTILIZATION IN TANGALE NATURE RESERVE GORONTALO PROVINCE Arini, Diah Irawati Dwi; Christita, Margaretta; Kinho, Julianus
BERITA BIOLOGI Vol 18, No 1 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Tangale Nature reserve is a conservation area located in Gorontalo that have the biodiversity typical of Wallacea bioregion including macroscopic fungi. The purpose of this study was to identify the fungal species diversity found in the Tangale Nature reserve as well as its potential use, considering that the information on species diversity of fungi in the Wallacea region is very limited. This research was conducted using the cruise method by identifying macroscopic fungi along the hiking trail of Tangale Nature Reserve. The macroscopic fungi were observed for its morphological characteristic include the colour, diameter, surface of the veil, the shape of the stem, the length and diameter of the stem, the lamella, including ring and pore, type of lamella and the type of volva. The research recorded the substrates and the location where the fungus was found. Data were analyzed descriptively qualitative. The results of the research identified 28 species of macroscopic fungi that included to the division Ascomycota and Basidiomycota. It belongs to 16 families and 8 orders are Pezizales, Agaricales, Auriculariales, Boletales, Cantharellales, Gomphales, Polyporales, dan Russulales. Based on place to grow as much as 57,14% were found growing on decaying wood and 42,86% found growing in the soil/litter. Based on the potential of use, it has identified four species of mushrooms potentially as edible mushroom are Pleurotus ostreatus, Auricularia auricula, Ramaria formosa, and Polyporus arcularius. 11 species of mushrooms potentially as medicinal mushroom are Calvatia craniiformis, Scleroderma citrinum, Lenzites betulina, Microporus flabelliformis, Coriolus versicolor, Microporus xanthopus, and Albatrellus confluens.

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