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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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EVALUASI KUALITAS BEBERAPA GENOTIPE BAYAM (Amaranthus sp) PADA PENANAMAN DI JAWA BARAT [Quality Evaluation of Some Genotype of Spinach (Amaranthus sp.) Cultivated in West Java] Rahayu, Suwarni Tri; Asgar, Ali; Hidayat, Iteu M; Kusmana, Kusmana; Djuariah, Diny
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.527

Spinach is one of leafy vegetables species that contain lots of vitamins and minerals. This study aims to examine the quality of several genotypes of spinach grown at two highland sites of West Java (Cipanas and Lembang).This experiment was conducted using Split Plot design,with five lines and five replications. Three genotype were tested, namely B1 = By16 , B2 = By18 , and B3 = By21, as well as two commercial varieties on the market spinach (B4 = red Giti , B5 = Kusuma).Measurements included physical parameters (length and width of leaves,stem diameter, texture, and color) and chemical parameters (water content, vitamin C, and fiber). The experiment was conducted in the laboratory of Physiology Vegetable Research Institute, Lembang from March to September 2012.The results showed that the content of vitamin C and fiber in genotype B1 and B2 were not significantly different from the control genotype.Similarly, the results of measurements of the length and width parameters of the leaves on all the tested genotype was not significantly different from the control genotype. Average length and width leaves of the genotype tested in Cipanas significantly higher than those tested in Lembang. Spinach has a shelf life of 1 day at room temperature in the storage area of Lembang ( 21Â±2Â°C and 85% RH ).

INDUKSI POLIPLOIDI DENGAN KOLKISIN PADA HIBRID F1 HASIL PERSILANGAN ANTAR SPECIES PADA TANAMAN PANILI ASAL CIAMIS Damayanti, Fitri; Mariska, Ika
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3455

Steem root disease caused by Fusarium oxysporum Schl. f. sp. Vanillae (Tucker) Gordon represent one internal issues of vanilla development (Vanilla planifolia Andrews). To obtain resistance clone to the disease, it can exploit resource of wild vanilla (V. albida B. L Syn) through crosses. Hybrids which were interspecific crossed generally were sterile. To overcome sterility problem of the hybrids, chromosome doubling was made by colchicine application. Explant used globular structure of proembryo from F1 seed result from a cross between wild vanilla of Ciamis as female parents and cultivated vanilla clone of Ciamis as male parent. Concentration level colchicine used were 0.00%, 0.05%, 0.10%, 0.20% and 0.25% with period of treatment of 3 and 6 days. After colchicine treatment embryo cultures were subcultured into new mwdium that was basal media Murashige-Skoog enriched with 2.5 mg/l BAP. Result of the experiment showed that colchicine treatment,globular structure were F1 embryo tending to inhibit early regeneration. The cultures showed variabilities from treatment of colchicin 0.20% during of 6 day and 0.25% for 3 days. Phenotypic performance of the chromosome doubled hybrids showing great variation in color and vigor of the culture. Tetraploid plant(2n=4x=64) was obtained from the colchicine treatment of 0.25% for 6 days. Chromosome addtion was followed by improvement of cell dimensions and organ magnification.

PENGARUH ARAH DAN UKURAN POTONGAN SISIK UMBI KERK LILY (Lilium longiflorum Thunb.) TERHADAP PEMBENTUKAN TUNAS MIKRO DAN BULBLET SECARA IN VITRO Priyono, Priyono; Winarsih, Sri
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mass production by using micro propagation technique has an important role for Lilium longiflorum regarding that it can produce seedlings in relatively short time, disease free, and regularly time. Several factors affected the successful micro shoots and bulblets formation, i.e.medium, explant, and temperature. This research aimed to know the effect of direction and width of scale section on the multiplication of kerk lily in vitro.Research has been conducted in the Tissue Culture Laboratory of Indonesian Coffee and Cocoa Research Institute, Jember.The research was arranged in factorial completely randomised design, i.e.direction of scale section and width of scale section. Two levels of direction of scale section namely longitudinal and transversal and five levels of width of scale section namely 1, 4, 6, 8, and 10 mm.The results showed that scales are able to produce micro shoot only, bulblet only, or both micro shoot and bulblet.The number of micro shoots produced from longitudinal section are higher than those of produced from transversal section. Width of scale section affected micro shoots and bulblets production, which their correlations are quadratic.The trend of micro shoots production was contrary with bulblet production.

PENGARUH EKSTRAK AIR DAN ETANOL Alpinia spp. TERHADAP AKTIVITAS DAN KAPASITAS FAGOSITOSIS SEL MAKROFAG YANG DIINDUKSI BAKTERI STAPHYLOCOCCUS EPIDERMIDIS SECARA IN-VITRO Wulansari, Dewi; Chairul, Praptiwidan
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Ellianol 70% and water exlracls of Alpinia spp. i.e. Alpinia zemmbei. A. kaisumadai. A. malaccensis and A. officinarum were examined for their impact in in-vitro phagocytosis activity and capacity of mouse (Mus musculus) peritoneum macrophage induced by Staphylococcus epidermidis.The extract concentrations used in this experiment were 0: O.I: 1.0: 10: 100: and 1000 Âµg/ml,Imboost (Echinacea pwpurea extract) 1000 Âµg/ml was used as positive control while distilled water as negative control.The assay results showed that all of the extracts were active to promote phagocytosis activity and capacity of macrophage cells.The phagocytosis acitivity and capacity were increased by increasing extract concentration, and ethanol extract showed better activity than water extract. Alpinia officinarum and A. kaisumadai extracts reveal better phagocytosis activity and capacity than others. Activity and capacity of phagocytosis of each concentration was significantly (P<0.05) different each other as well as with negative control. There is significant difference among each extracts and positive control at 1000Â Âµg/ml

SIRKULASI VIRUS AVIAN INFLUENZA SUBTIPE H5N1 DI PASAR TRADISIONAL DI JAWA TIMUR TAHUN 2012 [Circulation of the Avian Influenza Virus Subtype H5N1 at Traditional Markets of East Java in 2012] Hartawan, Risza; Dharmayanti, NLP Indi
BERITA BIOLOGI Vol 13, No 1 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Avian influenza virus subtype H5N1 outbreak has become endemic in Indonesia since 2003. The disease does not only cause immense economic losses but it also leads to significant fatality of human being. The existence of traditional markets including live bird trading is suspected to play important role in the spreading and evolution of the virus. The objective of this study was to identify the circulation of H5N1 virus at traditional markets of East Java in 2012 by using reverse transcriptase polymerase chain reaction (RT-PCR) test and virus isolation. As results, this study detected the presence of the H5N1 virus circulating in Gresik, Mojokerto, Lamongan and Surabaya in both of live birds and environmental samples. The successfulness of virus isolation indicated a potential transmission to other hosts, including to human. This study suggests that the improvement of the poultry trading system at traditional markets by implementing sanitation, hygiene and biosecurity is necessary to reduce the burden of virus contamination at the market environment.

ISOLASI, SELEKSI, DAN KARAKTERISASI MIKROBA PENDEGRADASI ASETONITRIL DARI LIMBAH INDUSTRI Sunarko, Bambang; Adityarini, Adityarini; Tambunan, Usman Sumo F; Sulistinah, Nunik
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A number of microbes which could grow on acetonitrile were isolated and selected from industrial effluents and were studied to characterise the isolate which has the best degrading capability.Cultures were grown on mineral medium with microelements and acetonitrile was added as sole source of energy, carbon, and nitrogen.Isolate D5, identified as Corynebacteriumsp.,was able to grow on high concentration acetonitrile (up to 5 % v/v) and exhibited the highest specific growth rate (j).When Corynebacterium D5 grew on 2 % (v/v) acetonitrile,the doubling time was 6 hours 40 minutes,the specific growth rate (p) was 0.1 h and the acetonitrile decreasing rate was 3.99 mM/h.Increasing of acetonitrile concentration would extend the doubling time, decline the maximum growth and specific growth rate (i), and biomass production of Corynebacterium 05.The products of acetonitrile degradation by Corynebacterium D5 were acetamide, acetic acid, and ammonia.The maximum growth of Corynebacterium D5 showed when /3-aminopropionitrile was used as a substrate.

KOMUNIKASI PENDEK ETNOBOTANI DAMAR PADA ORANG RIMBA DI TAMAN NASIONAL BUKIT DUABELAS [Ethnobotany of Dammar by Orang Rimba in Bukit Duabelas National Park ] Andhika, Rana Rio; Muhadiono, Muhadiono; Hilwan, Iwan
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i1.2863

Orang Rimba have local knowledge about utilization of dammar in Bukit Duabelas National Park .The research was aimed to record the type of dammar and explore local knowledge Orang Rimba utilizing dammar. Data were collected through interviews, participant observation and documentation . Data analysed as qualitative descriptive. There were 15 plant species whose resin were used by Orang Rimba i.e Dipterocarpus baudii, Dipterocarpus hasseltii, Hopea mengarawan, Hopea dryobalanoides, Dacryodes rugosa., Santiria laevigata, Shorea bracteolata, Parashorea aptera, Canarium pilosum, Shorea retinoides, Shorea cf. singkawang, Santiria dacryodifolia, Shorea multiflora dan Sarong (Trigona spp). Dammar were used Â as a fuel, medicine, adhesive material and traditional rituals. General use of dammar was for traditional Torch lighting (blebayon).

AKTIVITAS NITROGENASE BINTIL AKAR DAN KADAR KLOROFIL DAUN KACANG HIJAU {Phaseolus radiatus L.) PADA BERBAGAI JENIS DAN KADAR ASAM FENOLAT Rauf, AWahid
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.787

Phenolic acids are one of the many secondary metabolites causing toxic upon the growth and development of plant. Phenolic acids are produced by plant and subsequently released into soil, and appears to be involved in biochemical interaction between plants and other living organisms in soil. The research was conducted to study the effect of various kinds and doses of phenolic acids on nitrogenases activities and cholorophyll content of mungbean. Research was done in the glass house of Soil Science Departemen, Faculty of Agriculture, Gadjah Mada University (GMU) from April to June 2004. The experiment was arranged by randomized block design in three replications. The treatment consist of four kinds phenolic acid i.e. vanilic, p-coumaric, ferulic and p-1hydroxybenzoic with concentration at 250, 500, 1000 and 1500 mg kg-Ã respectively. One treatment was without phenolic acids as control. Result indicated that phenolic acids could reduce nitrogenases activities and chlorophyll content up to 53.35% and 29.98% respectively. The toxicity of p-hydroxybenzoic against both nitrogenases activities and chlorophyll content was higher than other phenolic acids.

PENERAPAN TEKNOLOGI FERMENTASI PADA BIOPROSES FERMENTASI MINYAK KELAPA (FERMIKEL) [Bioprocessing of Fermented Coconut Oil by Application of Fermentation Technology] Joko Sulistyo; Sulistyo, Joko; Soeka, Yati Sudaryati; Triana, Evi; Napitupulu, Rostiati NR
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Methods of extracting oil from coconut endosperm by fermentatbn were studied. The factors which must be controlled to break the emulsion and liberate oil were investigated. It was found that grinding conditions exerted a profound effect upon the stability of the coconut milk emulsion. The optimum condition for rapid fermentathn of coconut milk was related to the condition during incubation period. The fermentation progressed best under mild conditions (28Â°C-40Â°Cj. The fermentation was successful in breaking the emulsion at a relatively broad of range and titrable acidity. Coconut cream and small volume of coconut water and "lontar" (palmyra palmj-sap were incubated separately with some strains of Bacillus species, which were preincubated in a coconut tomato-extract sugar (CTSj medium using a shaker, and grown as a starter under conditions that allowed for coconut oil production at pH 4,0-5,0 and 30 CÂ°- 40 "C for 12-24 h. The organism destabilizes the emulsion, apparently by metabolizing sugars, resulting in the production of protein curd and high-quality oil. The palm sap and coconut water to the cream ratio of fermentation medium influenced the performance of oil produced and the bacteria grew well and produced oil in non sterile systems. The oil recovered was about 25 to 20% while average amount of oil in the coconut is approximately 25-35%, which means that only 83,33 to 66,67% oil was recovered. The oil contained little free fatty acid and very low concentration of cholesterol (0,0095 mg/ml), while the traditional coconut oil and commercially palm oil were 0,0111 mg/ml and 0,0132 mg/ml, respectively.

KERAGAMAN AKTINOMISETES KEPULAUAN WAIGEO, KABUPATEN RAJA AMPAT, PAPUA DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSADAN PELARUT FOSFAT Nurkanto, Arif
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Some Actinomycetes isolated from Waigeo Raja Ampat Regency Papua have been identified. Those isolates were also characterized for their cellulolitic and phosphate solubilizing ability. Microscopic identification was based on Miyadoh (1997) and Holt (1994) methods.Actinomycetes could be identified by microscopic observation on spores, chain spore, hypha, aerial hypha and its pigmentation.The cellulolitic ability was observed by clear zone ratio in CMC medium and phosphate solubilizing activity by the same approach in Vikoskaya medium.From 139 Waigeos Actinomycetes isolates which had been deposited in LIPI Microbial Collection (LIPIMC) were identified as 10 genera (Actinomadura, Actinoplanes, Microbiospora, Micromonospora, Nocardia, Pseudonocardia, Saccharopolispora, Streptomices, Streptosporangium and Thermomonospora). As much as 57.5% phosphate solubilizing actinomycetes and 82.7 % cellulolitic actinomycetes were detected.

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