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BERITA BIOLOGI

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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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POTENSI RUMPUT-RUMPUTAN UNTUK FITOREMEDIASI LAHAN TERDEGRADASI PENAMBANGAN EMAS Syarif, Fauzia; Juhaeti, Titi
BERITA BIOLOGI Vol 6, No 6 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Phytoremediation is defined as a clean up of pollutants primarily mediated by pliotosynthetic plants so its could be used as an alternative technique to overcome contaminated soil and water such an mining ecosystems. These plants have several beneficial characteristics; they have ability to accumulate metals in their shoots and especially high tolerance to heavy metals.Since Indonesia has abundant plant diversity, it is believed that some of them are potential to be used as phytoremediator.This research was carried out to study the potential of three species of grasses in accumulating Pb and Cn. The treatments were arranged on Randomized Complete Block Design (factorial design). The first treatment are species of grass: 1) Cynodon dactylon Pers., 2)Cyperus sp. and 3) Ischaemum timorense Kith.; the second treatment is planting media i.e. 1) tailing soil, 2) soil from wildmining (mining activities run by unexpected miners) and 3) top soil and the third is organic fertilizer: 1) manure, 2) compost and 3) no fertilizer.The resultsshowed that all of the three grasses are potential to accumulate Pb and Cn. Organic fertilizer mixed on planting media is significantly affected the growth of grasses and increasing the accumulation of Cn on leaf of Cyperus sp. and I.timorense. While no evidence in increasing Pb accumulation on all of those three grasses species

POTENSI ANTIBAKTERI TIGA SPESIES BAKTERI ASAM LAKTAT ASLI ENGGANO TERHADAP BAKTERI PATOGEN DAN PEMBUSUK MAKANAN [Antibacterial Potential of Three Indigenous Lactic Acid Bacteria Species from Enggano Against Pathogenic and Food Spoilage Bacteria] sulistiani, sulistiani; Khusniati, Tatik
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Lactic acid bacteria (LAB) strains have been known for their antimicrobial compounds that are important for food industry,including extension of expired time for food products. In this study, LAB isolates were isolated anaerobically from coconut sap using MRSA medium. Antibacterial analysis was carried out using microdilution method on microplate. The antibacterial assay showed that 85 isolates of LAB exhibit antibacterial activities against Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes, and Bacillus cereus. Molecular identification of 13 physiologically characterized LAB strains based on 16S rDNA sequence determined three species, namely, Leuconostoc mesenteroides (strain EN17-1, EN17-8, EN17-12, EN17-15, EN17-34, EN17-41, EN17-43, EN17-45, EN17-46, EN38-34), Lactobacillus fermentum (strain EN17-2, EN38-44) and L. satsumensis strain EN38-32. The strong and wide broad spectrum antibacterial activity was produced by L. satsumensis strain EN38-32.Â

METIL EUGENOL, KHEMOTIPE DARIMINYAK ATSIRI Melaleuca spp. (MYRTACEAE) YANG TUMBUH DIKEBUN RAYA CIBODAS Murningsih, Tri; Chairul, Chairul; Kuncari, Emma Sri
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The essential oils of the fresh leaves of Melaleuca spp. (M. alternifolia Cheel, M. ericifolia Sm., M. squarrosa Donn, and M.genistifolia Sm.) from Cibodas Botanical Garden, Indonesia were isolated by a hydrodistillation method and analysed by a gas chromatography/mass spectrometry (GC/MS) technique. The various Melaleuca species showed differences within their relative essential oil compositions. The oils from all samples were of the methyl eugenol (10.30-71.25%) chemotype. The others major constituent were terpinen-4-ol (38.49%), linalool (19.65%) and bis(2-ethylhexyl)phtalate (66.09%) in the M. alternifolia, M. ericifolia, and M. genistifolia respectively.

PENGGUNAAN Chaetoceros calcitrans, Thalassiosira weissflogii DAN KOMBINASINYA PADA PEMELIHARAAN LARVA UDANG VANAME (Litopenaeus vannamei, Boone 1931) [The Use of Chaetoceros calcitrans, Thalassiosira weissflogii and Its Combination to The Larval Rearing of Vaname (Litopenaeus vannamei, Boone 1931)] Panjaitan, Amyda Suryati; Hadie, Wartono; Harijati, Sri
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The use of one type live food in the larval rearing of vannamei shrimp is insufficient for maximum larval development. This research was aimed to evaluate the use of phytoplankton Chaetoceros calcitrans and Thalassiosira weissflogii and its combination as food to the growth and survivorship of white pacific shrimp Litopenaeus vannamei. The research used prawn larvae at stadia Nauplius4-5 with 150/ litre larval density. The larvae were fed and their effects with 3 kinds of live food, C. calcitrans (A), T. weissflogii (B), and combination of both types (C) for each treatment with five replications.The data was analysed using SPSSV.16. Result showed that the survival rate for treatment A was of 55.04+11.81%, treatment B was of 68.22+6.80%, and treatment C was of 77.04+4.63%. This indicated that treatment A gave significantly different on survival rate (P<0.01) than treatment B and C. Treatment B and C were not significantly different (P>0.05). We recomended the use of combination both of C. calcitrans and T. weisflogii to provide maximum survival rate for vannamei shrimp postlarvae.

KARAKTERISASI GEN PENYANDI PEDIOSIN PAF-11 PADA Pediococcus acidilactici F-11 [Characterization of the Pediocin PaF-11 Encoding Gene in Pediococcus acidilactici F-11] Marwati, Tri; Richana, Nur; Harmayani, Eni; Rahayu, Endang S
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pediocin PaF-11 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus acidilactici F-11. The objectives of this research is to find out the location and the nucleotide sequence of gene, which is involved in the production of pediocin PaF-11. Results showed that the pediocin PaF-11 from the cured cell of P. acidilactici F-11 loss the activity, suggested that the pediocin PaF-11 gene was carried in the plasmid. Agarose gel electrophoresis of P. acidilactici F-11 plasmid DNA with marker ÃÂ»DNA/HindIII showed that pediocin PaF-11 gene was carried in 12 kb plasmid. Amplification pediocin PaF-11 gene from P. acidilactici F-11 showed that uncured P.acidilactici F-11 culture contain plasmid DNA, indicated by amplification of the papA gene (256 bp). Cured P. acidilactici F-11 culture, plasmid eliminated, indicted by no aplicon DNA detected. This result also suggested that pediocin PaF-11 gene in P. acidilactici F-11 was carried in plasmid. Nucleotide of pediocin PaF-11 encoding gene was sequenced The alignment of that nucleotide sequence showed that pediocin PaF-11 encoding gene have the same sequence with pediocin PA.1 encoding gene in P. acidilactici PAC1.0 and P. acidilactici K10 and pediocin AcH encoding gene in P. acidilactici LB 42-923 and P .parvulus ATO77, and pediocin CP2 in P. acidilactici MTCC 5101.

KOMPOSISI DAN DOMINASI PATOTIPE Xanthomonas oryzae pv. oryzae, PENYEBAB PENYAKIT HAWAR DAUN BAKTERI PADA TANAMAN PADI DENGAN SISTEM PENGAIRAN BERBEDA DI KABUPATEN KARAWANG [The Composition and Domination of Xanthomonas oryzae pv. oryzae Pathotype, The Cause of Bacterial Leaf Blight on Rice Plants in Different irrigation System at Karawang District] Yuliani, Dini; Natasuwirya, Sudir
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Bacterial leaf blight (BLB) is the major disease of rice caused by the bacteria Xanthomonas oryzae pv. oryzae (Xoo), that has various pathotypes making it difficult to control. This research was aimed to determine Xoo pathotype group in irrigated paddy fields with different of planting time based on differences of irrigation system in Jatiluhur Reservoir. The research was conducted in Karawang District the wet season (WS) of 2013/2014 and dry season (DS) of 2014 through survey and sampling of infected rice leaves of BLB. Isolation of Xoo bacte-ria was done in the laboratory by using dilution methods. Xoo pathotype test was carried out by inoculating Xoo isolate on five different varieties in Indonesian Center for Rice Researchâs green house, West Java. Observation of disease severity was done by measuring symp-toms of the disease that appeared at two and four weeks after inoculation. The disease severity of ?11% was classified as resistant (R), and for the disease severity of >11% was classified as susceptible (S). The results obtained a total of 602 Xoo isolates from rice plant samplings of irrigated rice fields in Karawang with irrigation systems I, II, III, and IV. At WS 2013/2014 was obtained 448 Xoo isolates consists of 29.69% of pathotype III; 40.85% of pathotype IV, and 29.46% of pathotype VIII. While, at DS 2014 was obtained 154 Xoo isolates consists of 1.95% of pathotype III, 53.25% of pathotype IV, and 44.80% of pathotype VIII. The areas with Type I of irrigation systems were domi-nated by Xoo pathotype IV in two cropping seasons. The areas with Type II of irrigation system dominated by Xoo pathotype IV in the wet season, while the dry season was dominated by Xoo pathotype VIII. The areas with Type III of irrigation system were dominated by Xoo pathotype III in the wet season, whereas during dry season was dominated by Xoo pathotype IV. The areas with Type IV of irrigation sys-tem were dominated by Xoo pathotype IV during wet season. Over all, in the dry season, Xoo pathotype IV was the dominant pathotype especially in irrigation systems Type I and IV. The dominance of Xoo pathotype can determine the resistant varieties that grown in the areas with different planting times on different irrigation systems.

PENGARUH PENAMBAHAN JUMLAH INOKULUM DAN DI-KALIUM HIDROGEN FOSFAT PADA FERMENTASI PRODUKSI DEKSTRAN Triantarti, Triantarti; M, Hendro Santoso
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Dextran production is conducted by fermentation by using Leuconostoc mesenteriodes wich produces dextransucrase enzyme.Sucrose is converted to dextran by dextransucrase. Sucrose is a main carbon source in dextran fermentation. Hence, sugar cane juice mainly contains sucrose is potential material for dextran fermentation. The effect of inoculum concentration added at the beginning of fermentation of di-potassium hydrogen phosphate concentration in the medium were studied. L.mesenteroides B-512F was used. The results showed that there were no effect on optimum growth and dextran production when the inoculum concentration added at 1% and 5%(w/v). The only difference was inoculum at 1% (w/v) delaying the growth and dextran formation in comparison to the addition of 5%(w/v) inoculum. The optimum growth and dextran production were affected by di-potassium hydrogen phosphate concentration in the medium (0,5, 1,0 and 1,5% w/v).The growth was highest at di-potassium hydrogen phosphate concentration 1,5 % w/v. On the otherhand, dextran production was lower compared to the other treatments.

ANALISIS TINGKAT PENCEMARAN AIR DENGAN METODE INDEKS PENCEMARAN DI TELUK YOUTEFA, JAYAPURA, PROVINSI PAPUA Manalu, Janviter; Nurjaya, I Wayan; HS, Surjono; Kholil, Kholil
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1942

Youtefa Bay is one of the potential natural recources, is located on the western side of Jayapura City,Papua province.The waters has beenutilized for fishery, transportation and ecotourisme.Youtefa Bay surroundings are settlements where the most of residents are fishermen and farmers.The aim of this study is to determine the pollution level of the Youtefa Bay,approached by pollution index method.The results showed that the water condition of Youtefa Bay was slightly to moderately polluted.Due to pollution, it is therefore very importance to periodically monitor the water quality of the Youtefa Bay to maintain its sustainability.

PERKEMBANGAN EMBRIO DAN LARVA IKAN PATIN NASUTUS (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) [Embryonic and Larval Development of Patin Nasutus (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces)] Iswanto, Bambang; Tahapari, Evi
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Patin nasutus (Pangasius nasutus Bleeker, 1863) (Pangasiidae; Pisces) is a potential pangasiid catfish species to be cultured in Indonesia.Many efforts has been done to explore the potency of P.nasutus for aquaculture purposes.However, basic knowledge is still very rare such as its embryonic and larval development.The present study aimed to understand the embryonic and larval development of P. nasutus resulted from induced spawning of P.nasutus brooders at Research Institute for Fish Breeding, Sukamandi. Those embryonic and larval development were observed microscopically.The results showed that diameter of the ovulated oocytes ranged 1.40-1.65 mm and fertilized eggs ranged 1.90-2.15 mm.The embryogenesis consisted of several stages as follow: morula, blastula, gastrula, organ developments and newly hatched larvae occured within periods of (minutes) 20-200, 190-220, 210-660, 600-1,140 and 1,800-2,160 after fertilization at water temperature of 28-29oC. Total length of the newly hatched larvae ranged 4.80-5.10 mm with anterior, posterior and dorsal parts of the yolk sac coloured black pigmentation. On caudal peduncle, the black spot existed since 36 hours after hatching was a strong character for P.nasutus larvae.Volume of the yolk sac was 2.65Â±0.14 mm3, for which 50% absorbed at 36 hours after hatching and relative fully absorbed at 78 hours after hatching. The larvae have complete morphology as adult after seven days. During early developmental stage, larvae showed cannibalism since the third day, corresponding to the first exogenous feeding.

PERBANYAKAN DAN PENYIMPANAN KULTUR SAMBUNG NYAWA [Gynura procumbens (Lour.) Merr.] DENGAN TEKNIK IN-VITRO Hoesen, Djadja Siti Hazar
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Sambung nyawa [Gynura procumbens(Lour.)Merr.]is one of the traditional medicine sources.The plants are not cultivated intensively in the field. One of the efforts to maintain and to propagate this species is by multiplication through tissue culture method. Nodes explants were cultured in MS normal and half strength concentration macroelements, supplemented with microelements and vitamins; combination of cytokinin BA (2 mg/1), thidiazuron (0.01 mg/1 and 0.1 mg/1) and adenine sulphate (5 mg/1).Auxin (2,4D 0.5 mg/1) in combination were added in media as treatments and activated charcoal (2 g/1) as antioxidant. Young leaves explants were also cultured in the same basic medium (MS and A MS) in treatments with cytokinin (BA and thidiazuron).The results from nodes and young leaves explant indicated that the highest number of survival cultures were obtained from combination between BA (2 mg/1) and thidiazuron (0.01 mg/1) in MS normal strength basic medium.In acclimatization stage, 100% of plantlets survived and successfully transplanted to soil medium in the field; maintenance study indicated that subculture was prolonged until 52 weeks.

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