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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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KERAGAMAN GENETIK, HERTTABILITAS DAN KORELASI BEBERAPA KARAKTER AGRONOMI PADA GALUR F2 HASIL PERSILANGAN KACANG HIJAU (Vigna radiata (L.) Wilczek) [Genetic Variability, Heritability and Correlation of some Agronomic Characters in the F2 of Varietal crosses of Mungbean {Vigna radiata (L.) Wilczek)] Hakim, Lukman
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The F2 progenies of two crosses among three mungbean varieties were evaluated with their parents (Varsha, Park it and Local Belu)at Muara Experimental Station, Bogor during dry season of 2007. The experiment was conducted in a randomized complete block design with three replications. The seed of each F2 progenies and parents were planted in four rows of four meters long.Plant spacing was 40 x 20 cm, each will contained one plant. Among the character studies, days to maturity, seed yield per plant and plant height had the highest coefficient of variability, with the mean of 51.3%, 49.8%, and 49.2% respectively.Number of branches, pod length and number of seed per pod had the lowest coefficient of variability, and the mean were 19.1%, 18.8%, and 17.6%.The heritability estimates of nine agronomic characters studied ranged from IS.6 to 65.2%. Plant height and number of pods per planthad the highest heritability estimates with mean of 65.2% and 58.6% respectively for the two crosses. The mean heritabilityestimate for seeds yield per plant, pod length and number of seeds per pod were the lowest of 19.4%, 18.3% and 15.6% respectively. Based on the F2 data, selection of plant height and days to maturity had the highest expected genetic advance of 45.8 and 41.0%.The mean expected genetic advance for pod length and number of seeds per pod were the lowest of 19.4 and 17.4%.Correlation coefficient between grain yield with plant height, number of pods per plant and seed size were positively significant (r = 0.404, 0.613 and 0.422 respectively). Correlation coefficient between grain yield with other agronomic characters were small or negative. Therefore, plant height, number of pods per plant and seed size can be used as the selection criteria in selecting mungbean genotypes for high yield.

IDENTIFIKASI 26 ISOLAT BAKTERI ENDOFITIK DAN FILOSFER PADI DENGAN ANALISIS SEKUEN16S RDNA Hidayatun, Nurul; Susilowati, Dwi N; K Mulya, K Mulya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The research was subjected to identify 26 endophytic and phyllosphere bacteria isolated from 4 rice varieties using sequencing method of 16S rDNA.The sequences were then aligned with reference sequence from Gen-Bank data library by BLAST program from NCBI to find the most related identity of the isolates analysed.The sequencing analysis revealed that some isolates have a high identity to Staphylococcus and Serratia (5 isolates each), Bacillus (4 isolates), Microbacteria (3 isolates), Pseudomonas (2isolates) and Klebsiella, Acidovorak, Bulkholderiaceae, Agrobacterium and Shewanella (1 isolate each). No specific isolate dominated in both endophytic and phyllosphere group, nor host-specific isolate found in the four rice varieties.

AMFIBIA DAN REPTILIA CAGAR ALAM GUNUNG SUPIORI, BIAK-NUMFOR: DAERAH KORIDO DAN SEKITARNYA Kurniati, Hellen
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

During three weeks field work survey (16th June-7 July 1999) on seven habitat types in Gunung Supiori Nature Reserve, Biak-Numfor District, Papua 21 species of reptiles (six species of geckos, nine species of skinks, one species of monitor lizard and five species of snakes) and two species of amphibians were found.On all of identified species, no one is endemic species in Supiori Island, although the island has drifted for 200 million years ago.The phenomena has been occurring in this area is tend to be Brown Phenomena (Brown, 1997).Based on information from local people, two species of sea turtles (Chelonia mydas and Eretmochelys imbricata) occur barely in surrounding waters and population status of Crocodylus porosus the species that inhabitant in mangrove has already been extinct since 1980-year era.Population status of reptiles that listed in Appendix II CITES, such as Candoia aspera and some species of reptiles and frogs that listed as non-appendix CITES are almost still in good condition; the good population status is also occur on protected monitor lizard Varanus indicus.

KEANEKARAGAMAN KHAMIR YANG DIISOLASI DARI SUMBER DAYA ALAM PULAU ENGGANO, BENGKULU DAN POTENSINYA SEBAGAI PENDEGRADASI SELULOSA [Diversity of Yeasts Isolated From Natural Resources of Enggano Island, Bengkulu and Its Cellulolytic Potency] Sumerta, I Nyoman; Kanti, Atit
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2220

The present study revealed the occurrence and diversity of yeasts from natural resources of Enggano Island. The yeasts were isolated from soil, sediment, leaf, leaf litter, fruit, and flower. Isolation was performed using direct plating, membrane filtration, ballistospore-falling and enrichment culture using glucose, xylose and xylan as carbon source. After isolating, yeasts were screened for its cellulolytic potency. The occurrence of yeasts in different isolation technique are clearly found. Most of 87 yeasts isolated, abundant in leaf, soil, and sediment sample. The total yeasts isolated were classified into 32 species based on D1/D2 LSU 26S rDNA sequences analysis which genera of Candida were predominant (33%). The predominant yeast species were identified as Candida tropicalis (16,1%), Cyberlindnera saturnus (16,1%), and Rhodosporidium paludigenum (11,5%). Screening on carboxymethyl cellulose medium, there are 43 isolates within 22 yeasts species have cellulolytic activity. Four species of Basidiomycetous have high activity which includes Pseudozyma antartica Y15Eg001; Pseudozyma hubeiensis Y15Eg015; Anthracocystis chrysopogonis Y15Eg072; Cryptococcus laurentii Y15Eg017 and two from Ascomycetous are Sarocladium bactrocephalum Y15Eg226, Y15Eg227. Some of cellulolytic yeast isolates are indicated as new species candidate and required to reconfirm in another conserve regions to ensure its taxonomic position. Various yeasts isolated from Enggano Island with its cellulolytic potency should contribute to scientific information regarding microbial genetic resources of outer islands of Indonesia.

MUNDU: Garciniaxanthochymus Hook.f. ATAU G. dulcis (Roxb.) Kurz. Utami, Nanda; Sari, Rismita
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i6.850

"Mundu" is common name for one of the member of Clusiaceae family (manggis-manggisan).In scientific writing it is sometimes called Garcinia xanthochymus Hook.f. or G. dulcis (Roxb.)Kurz. To determine the correct name for these two kinds of plant a study was conducted to review its taxonomic status. Based on morphological data, anatomical and phylogenetic analysis it is showed that the two species is separated but there are closely related, and in according to International Code of Botanical Nomenclature Garcinia xanthochymus Hook.f is the correct name for "MUNDU"

KARAKTERISASI GALUR HIBRIDA HASIL PERSILANGAN IKAN GURAMI (Osphronemus goramy Lac.) ASAL JAMBI, KALIMANTAN SELATAN DAN JAWA BARAT BERDASARKAN METODE TRUSS MORFOMETRIK [Hybrid Strain Characterization Result of Crossbred Giant Gouramy (Osphronemus goramy Lac.) Origin of Jambi, South Kalimantan and West Java Based on Morphometric Truss Method] suharyanto, suharyanto; Febrianti, Rita; Sularto, Sularto; Abimanyu, Ade Anom
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i1.2845

Giant gouramy is a native fish to Indonesia and widely distributed in Sumatra, Kalimantan and Java. The aim of this study was to characterize the phenotype and kinship of Giant Gouramy from crosses between strains Jambi, South Kalimantan, and West Java (Tasikmalaya). This Research was conducted at the Giant gouramy Hatchery of Research Institute for Fish Breeding, Sukamandi, West Java. Analysis of morphological diversity of all crosses between varieties performed through morphometric measurements that have been determined based on a point benchmark of 16 haracteristics of the truss. Measurement data of each character crosses which have beenrelativized by standard length were analyzed using discriminant analysis and hierarchical cluster analysis. The result showed that Giant gouramy has low diversity coefficient values, ranging from 4 to13%. The highest coefficient of variance (CV) was found in the character of C3 (the tip of the anal fin-base of the tail fin), which ranges from 18-33% and the lowest in the character B5 (the base of the pelvic fin-tip of the dorsal fin) in the range of 2-4%. Based on discriminant analysis, males Giant Gouramy have more character differences than females. The closest kinship relationship is in hybrid of Tasikmalaya and Jambi (TJ) strain with of South Kalimantan and Tasikmalaya ( KT) which has a similarity level at 89.41%. However, the farthest kinship is in pure line of Tasikmalaya (TT) and Jambi (JJ) with the three other strains namely South Kalimantan and Jambi (KJ), Tasikmalaya and Jambi (TJ) and South Kalimantan and Tasikmalaya (KT) with a value ofsimilarity of 53.93%.

PERTUMBUHAN IKAN BANDENG (Chanos chanos) ANTARA BENIH HSRT DAN GENERASI KEDUA (G-2) TERSELEKSI [Growth Performance of Milkfish, (Chanos chanos) Between Small Scale Hatcheries and of Selected Second- Generation (G-2) Sources] Kusumawati, Daniar; Jamaris, Zafran; Aslianti, Titiek
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Currently, there is a national concern regarding to the decrease of milkfish production from ponds in North Java due to a low quality ofÂ milkfish seed produced by small scale hatcheries in Bali, which is the main producer of milkfish seed. The quality of seed is strongly relatedÂ to the quality of eggs (fertility), while quality of eggs produced depends on the management of brood stocks carried out by theÂ hatcheries. Growth rate and growth hormone profiles are some of the biological aspects that could be used as a basis/benchmark to evaluateÂ quality level of milkfish seed reared in ponds. The aim of this experiment was to evaluate growth rate and growth hormone profileÂ performance between selected G-2 seed and small scale hatcheries seed as control population the growth rate. Research on grow-out ofÂ milkfish seed was conducted at the IMRAD ponds facility in Pejarakan, using milkfish seed produced by small scale hatcheries as well asÂ selected second-generation (G-2) seed, each with the density of 5000 seed/pond (1 pond=0.5 ha). The seeds were fed with dry pellet andÂ reared for 5 â 6 months. The results showed that the seed produced with standard operational procedure (SOP) by small scale hatcheriesÂ were having longer (F hit. = 13.68 > F tabel 1%) and heavier body washt (F hit. = 18.98 > F tabel 1%) better than selected G-2 seed andÂ small scale hatcheries seed without SOP with high growth hormone concentration (F hit. = 4.95 > F tabel 5%).

KAJIAN ETNOBOTANI RAMUAN PASCA MELAHIRKAN PADA MASYARAKAT ENGGANO [The Ethnobotanical Study of Postpartum Concoction on Enggano People] Royyani, Mohammad Fathi; Lestari, Vera Budi; Agusta, Andria; Efendy, Oscar
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This research was aimed to discover the traditional knowledge of Enggano people when using plants as concoction after giving birth. Data was collected through in depth interview, open-ended, and literature studies. The results showed that Enggano people used specific plants as concoction in childbirth process. There were two types of the concoction. The first was used after giving birth to 40 days, jarak pagar or tiarak (Jatropha curcas L.). The second was used after 40 days to 60 days or after recovery, such as dukung anak (Phyllanthus niruri Linn.), kumis kucing (Orthosiphon stamineus Benth.), temu lawak (Curcuma xanthoriza Roxb.), alang-alang (Imperata cylindrica (L) P. Beauv.), banana (Musa sp.), kencur (Kaempferia galanga L.), turmeric (Curcuma longa L.), and ginger (Zingiber officinale Roscoe). Results from our literature studies confirmed the efficacy of plants used by Enggano people is scientifically acceptable. Further study on phytochemical aspects of these medicial plants is therefore required to be carried out.

AKTIVITAS ANTIBIOFILM BAKTERI Escherichia coli OLEH BAKTERIOFAG SECARA IN VITRO [Escherichia coli biofilm in vitro eradication by bacteriophage] Triana, Evi
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Several Escherichia coli strains are pathogenic. Excessive and noncompliant use of antibiotics and disinfectants may cause bacteria to build resistance mechanisms. Forming biofilms cause eradicatation more difficult. An effective cleaning action required antibiofilm and antimicrobial agents that have different mechanisms with antibiotics and disinfectants. Bacteriophages are potential candidates because they meet these requirements. Bacteriophages produce specific polysaccharide lyase enzymes capable of degrading biofilm extracellular polymeric matrix. Study was aimed to determine concentrations of specific bacteriophage showing Escherichia coli antibiofilm activity was conducted. The results of this study showed that the most effective concentrations bacteriophage EC RTH 04 to prevent, inhibit, and degrade Escherichia coli EC 3 biofilms were 106, 102, dan 102 respectively.

KERAGAMAN IKTIOFAUNA MUARA SUNGAI CIMANUK, INDRAMAYU, JAWA BARAT [Ichthyofaunal Diversity of Cimanuk Estuary, Indramayu, West Java] Tampubolon, Prawira ARP; Ernawati, Yunizar; Rahardjo, M.F.
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Cimanuk river, which the estuary formed a delta, is a habitat for many fishes that occupied the water in northern coast of Ja va. The estuary is essential for supporting the fish life cycle. The aim of this study was to asscess the diversity of ichthyo fauna in Cima nuk River estuary. The fishes were collected in three months from July to September 2013 at three locations: Pagirikan, Pabean Ilir and Song. Total fish collected were 1,826 individuals, consisted of 103 species from 41 families and 14 orders. Most of them were from Family Ambassidae,Leiognathidae, Scianidae, Gobiidae, and Ariidae.

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