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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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KARAKTERISASI GENETIK IKAN LELE DUMBO BERDASARKAN MARKER RAPD FINGERPRINTING [Genetic Characterization of African Catfish Revealed by RAPD Fingerprinting Markers] Nugroho, Estu; Putera, Sabara
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

African catfish, namely lele dumbo, is an economically important fresh water fish in Nusa Tenggara Barat. There are three strains of African catfish that are well distributed among farmers. Genetic information of three strain catfish stocks is needed in order to sustain their development program. Genetic variations of the three strains of catfish, i.e. Sangkuriang, Masamo, Paiton has been evaluated using RAPD markers. DNA genome was extracted using phenol-chloroform methods. DNA was amplified using 20 primers (OPA 1 â 20). The results showed that three of the 20 primers have good amplification products. There was no significant genetic differences among three strains of catfish analyzed (P>0.05). Genetic variation of three African catfish stocks ranges from low to middle value. The highest genetic variation is found in strain Masamo with heterozygosity of 0.273 (with 70% polymorphism loci), followed by Sangkuriang 0.189 (60%) and Paiton0.147 (40%). Hybrid candidates of Masamo-Sangkuriang have eterozygosity ranges between 0.256 - 0.306 (with 66.6 7 â 73.77% polymorphism loci). Strain Masamo and Paiton has more close genetic relationship than among the both and Sangkuriang.

KEANEKARAGAMAN, PERSEBARAN DAN POLA TATA RUANG TUMBUHAN EPIFIT PADA HUTAN BEKAS TEBANGAN DI KIYU, PEGUNUNGAN MERATUS, KALIMANTAN SELATAN [Diversity, Distribution and Spatial Patterns of Epiphytic Plants at The Logged Over Forest in Kiyu Forest, Meratus Mountain, South Kalimantan] Sadili, Asep; Royyani, Mohammad Fathi
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A quantitative study of epiphytic plants was conducted at the logged over forest in Kiyu, Meratus Mountain, Hulu Sungai Tengah, SouthÂ Kalimantan. This study used a plot of 10 m x 500 m (Â± 0,5 ha), and divided into 50 subplot of 10 m x 10 m. The results showed that thereÂ were nine species of six genera from three families of epiphytic plants found within the studied plot, with A grostophyllum bicuspidatum asÂ the dominant species. The diversity index (Hâ) was of 1,85. The diversity of epiphytic plant in these plots was well represented based on theÂ results of analysis of species area curve. Spatial distribution pattern of all epiphytic species was homogeneus, except forÂ A. bicuspidatum. The number of epiphytic host plants observed was 22 individuals from six species of six genera, and five families, withÂ Saurauia nudiflora (Acthiniaceae) was the dominant host plant.

POTENSI VEGETASI DAN DAYA DUKUNG UNTUK HABITAT GAJAH SUMATERA (Elephas maximus sumatranus) DI AREAL PERKEBUNAN SAWIT DAN HUTAN PRODUKSI KECAMATAN SUNGAI MENANG, KABUPATEN OGAN KOMERING ILIR [Vegetation Potency and Carrying Capacity for Sumatran Elephant (Elephas maximus sumatranus) Habitat at Palm Oil Plantation and Forest Production Area in Sungai Menang Districts, Ogan Komering Ilir Regency] Setiasih, Garsetiasih; Rianti, Anita; Takandjandji, Mariana
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Forest land converted into palm oil plantations have caused habitat fragmentation of elephant and land degradation. These lead to land use conflict between human and elephant. The conflict often caused the elephant killed and destructed agricultural land. The study was aimed to estimate potential use and carrying capacity of elephant habitat. Data collection of undergrowth vegetation were analyzed using twelve square plots of 1 x 1m, the distance between the plot of 50 m, tree vegetation of seedlings size 1 x 1 m, saplings 5 x 5 m, and trees 20 x 20 m, the distance between the plot of 200 m and of 1000 m lenght. Vegetation used as elephants feed were observed using purposive sampling and systematically procedure. The analysis showed that biomass of plants producing elephant fodder in Tambang Besi were of Cyperus rotundus (3600.26 kg/ha), Cynodon dactylon (346.74 kg/ha), Melaleuca leucadendron (255.21 kg/ha), and Melastoma malabatricum (156.40 kg/ha). While, the highest biomass in Tebing Penigasan plot is Cyperus rotundus (3575 kg/ha), and in Barak Gajah Plot is Isachne globusa (4013.33 kg/ha). The carrying capacity of elephants habitat of Tambang Besi, Tebing Penigasan, and Barak Gajah plots are 0.78, 0.29, and 0.41 individual/ha/day, respectively.Â

PENGARUH SUMBER PUPUK ORGANIK TERHADAP PENAMPILAN TEBU (Saccharum officinarum L.) PADA TATA TANAMBARIS GANDA BENIH GANDA [Effect of Organic Fertilizer Resources on Sugarcane (Saccharum officinarum L.) Performances in Double Rows Double Seeds Planting Arrangement] Busro, Djumali; Mulyaningsih, Sri; Basuki, Teger
BERITA BIOLOGI Vol 17, No 1 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Increased productivity of sugarcane in upland area can be done through the implementation of the integration of manure with a high dose of inorganic fertilizer in the double rows and twice plant populations of single row (âdouble rows double seedsâ) planting arrangement. Limited availability of manure and inorganic fertilizer entails the replacement of manure with green manure fertilizer. The research wasÂ aimed to obtain a green manure as replacement manure that can be intercropped in the double rows double seeds planting arrangement. The experiment was done in the Asembagus Experimental Station, Situbondo, East Java on January to December 2014 for plant cane (PC) and from January to December 2015 for ratoon cane (RC). Treatments were arranged in a Randomized Block Design time for replication. The treatment includes (1) the four rows of Crotalaria juncea, (2) two rows of Crotalaria juncea, (3) four rows of peanuts in width between rows of sugar cane, (4) manure, and (5) no organic fertilizer. The results showed that the integration of organic fertilizer with a low dose of inorganic fertilizers increases productivity and sugar yield 5.20 to 41.78% and 33.33 to 69.90%, respectively in PC and 7.33 to 32.67% and 5.74 to 29.61%, respectively in RC from no organic fertilizer. Four rows of Crotalaria juncea could replace the role of manure in improving the productivity and sugar yield for PC and two rows of Crotalaria juncea for RC.

ANTIPROLIFERATIVE ACTIVITY OF OCTADECA-8,10,12-TRIYNOIC ACID AGAINST HUMAN CANCER CELL LINES [Antiproliferasi Asam Oktadeka-8,10,12-triunoat Terhadap Galur Sel Kanker Manusia] Winarno, Hendig
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Antiproliferative activity test of octadeca-8,10,12-triynoic acid isolated from parasitic plant Scurrula airopurpurea (Bl.) Dans,against four kinds of human cancer cell lines, i.e: HeLa (human cervix epitheloid carcinoma),leukemia THP1,(human peripheral blood acute monocyte).carcinoma A549 (human lung carcinoma) and lymphoma HUT78 (human cutaneous T-cell lymphoma)was carried out.The results showed that octadeca-8.10,12-triynoic acid exhibits the antiproliterative activity against four kinds of human cancer cell lines with the 1C5I, value of 0.66. 0.86, 0.99 and 2.36 mg/nil for HeLa.leukemia THP1, lung carcinoma A549,and lymphoma HUT78,respectively, lower than 4 mg/ml. which is the antiproliferative activity threshold for pure isolate or compound.

EFEKTIVITAS MULTIVITAMIN DAN MENIRAN (Phyllanthus neruri L.) DALAM MENURUNKAN STRES PADA DOMBA SELAMA TRANSPORTASI Satyaningtijas, Aryani S; Andriyanto, Andriyanto; Ramadhoni, Armando; Suci, Yulia; Dewi, Fitriana; Sutisna, Abadi
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Sheep is one of achievable animal protein sources. Therefore, they need to be transported to/in different places to fulfill the demand of mutton. This research was conducted to know the impact of transportation on stress as presented by leucocyte differentiation (N:L ratio). Blood were taken from sheep without stress transportation as a control for 12 hours.In this research, 12 sheep were divided into 3 groups: KP (control with stress),PA (stress with combination multivitamin and meniran - Phyllanthus niruri L.), PB (stress with combination multivitamin).Blood samples were taken at 0 hour (pre-transportation), 4h, 8h, 12h (during transportation) and 24h, 48h, 72h (post-transportation) to observe leucocyte differentiation. The results showed that ratio N:L (as stress indicator) were higher at all time periods during transportation and after the transportation. Sheep as a control group had reached the peak of ratio N:L at 0 h, meanwhile sheep with combination of multivitamin and meniran at 12 h and sheep with multivitamin only at 8. It means that the sheep with combination of multivitamin and meniran treatment was more effective to response the transportation stress.

POPULASI POHON JENIS DIPTEROCARPACEAE DITIGA TIPE HUTAN PAMAH KALIMANTAN Simbolon, Herwint
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Data of Dipterocarpaceae species have been extracted from 8 studied plots of 1-ha each, distributed in three types of natural forest in Kalimantan (those were 4 plots in mixed dipterocarps forest, 2 plots in peat swamp forest and 2 plots in heath forest types) for further population analysis. Number of Dipterocarpaceae species in mixed dipterocarps forest type was higher than of in two other forest types, however mean number of individual per species was higher in peat swamp and heath forest types than of in mixed dipterocarps forest type. Some of Dipterocarpaceae species are adapted to heath and peat swamp forest type conditions indicated by the number and distribution of population of the species in both forest types. The higher the population number of juvenile trees 5-10 cm in diameter, the lower population number of mature trees of diameter 50cm up, indicated the higher tree population the higher mortality rate. However, survivorship of tree will higher as the tree diameter higher. Survivorship of trees in mixed dipterocarp forest is higher than of in peat swamp and heath forest types.

STUDI VEGETASI HUTAN RAWA AIR TAWAR DI CAGAR ALAM RIMBO PANTI, SUMATERA BARAT Yusuf, Razali; Purwaningsih, Purwaningsih
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i5.1986

A Vegetation study of some different freshwater swamp forests in Rimbo Panti Nature Reserve, West Sumatera was carried out with a quadrat methode.The results of a floristic inventory of trees with DBH e" 10 cm show that 136 species of 90 genera in 402 families ,presented by 1676 individuals, with the total basal area of 53.11 m occurred in the 5 hectare sample plots.The two leading families in terms of number of species were Euphorbiaceae and Lauraceae while according to the the total sum of importance values for families were Euphorbiaceae, Moraceae and Bignoniaceae.We recorded 19 species of Euphorbiaceae, constituting14.4% of the total species with the basal area of 39.4 % of the total in the three plots. Terminalia copelandii(Combretaceae) was the most prominent species occurring here and was one of the ten leading species. The species-area curves rose steadily up to an area of 1.0 hectare, with a very slight indication of levelling off at about 2 hectares, indicating high Heterogenity particulary in the permanently flooded swamp forest. Four largest trees were Artocarpus rotundatus (DBH = 100.50 cm) Chydenanthus excelsus (DBH = 92.50 cm), Haplophragma macrolobum (DBH = 83.00) and Anthocephalus chinensis (DBH= 75.10 cm).

KILAS BALIK PENELITIAN KROMOSOM PALEM INDONESIA Witono, Joko Ridho
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

There are 525 native palm species to Indonesia.Chromosome numbers of 61 Indonesian palm species have been reported,unfortunately, not all species were presented by their karyotype. Re-observation of palm chromosomes is needed, because mis identification of particular species and uncorrect chromosome counts might be happen in the past. Chromosome characteristics of some families, such as Poaceae and Onagraceae were proved useful for classification.On palms, almost all species within similar genera were known had similar chromosome numbers and different in chromosome forms and sizes.Trends to employ chromosome characteristics for palm classification is possible.Since, chromosome information on palm is limited,chromosome research has great challenge to do in the future.

RESVERATROL 10C-GLUKOPIRANOSIDA DAN HEIMIOLA, KARAKTER Hopea gregaria SECARAKEMOTAKSONOMI Sahidin, Sahidin; Hakim, EH; Syah, YM; Juliawaty, LD; Achmad, SA; Din, LB; Latip, J
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Ten resveratrol oligomers have been isolated from the stem bark of H. gregaria i.e. resveratrol lOC-glucopyranoside (1), heimiol A (2), balanocarpol (3), ampelopsin A (4), hopeaphuran (5), E-viniferin (6), parviflorol (7), a-viniferin (8), hopeaphenol (9), and vaticanoi B (10). The structures of these compounds were determined base on spectroscopic methods including UV, IR, 1-D NMR, 2-D NMR and comparison with the reported data. Implication of the compounds invention is not only strengthen conclusion that Hopea tends to produce resveratrol dimmers but also showed that resveratrol lOC-glucopyranoside (1) and heimiol A (2) are phytochemical markers of. H. gregaria.

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