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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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KERAGAMAN BAKTERI ENDOFITIK DnSOLASI DARI EMPAT VARIETAS PADI DENGAN METODA ARDRA Susilowati, Dwi N; Hidayatun, Nurul; Tasliah, Tasliah; Mulya, K
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1978

Sixty eight endophytic bacteria were isolated from four different rice varieties (IR64, Cirata, Code and Limboto) obtained from agroecosystem in Cikembar, Sukabumi, West Java. Those isolates were subjected for analysis the diversity based on genetic fingerprinting through Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. The objective of this research is to characterize the predominant endophyte bacteria are present within various rice varieties grown on agroecosystem in Cikembar, Sukabumi by using ARDRA method. The results shows that restriction analysis with both Rsal and HaelH was sufficient to allocate the endophyte bacteria from four different rice varieties into the 29 types. Moreover, Rsal alone was capable of resolving the 11 types, followed by HaelH 14 types. In general, the result may explain that there is no collinierity between the cluster and their host plant. Staphylococcus, Bacillus, Agrobacterium, Serratia, Klebsiella, Acidovorax and Pseudomonas were identified as endophytic bacteria from rice varieties in this agroecosystem based on their 16S rRNA sequences. Seven types were placed in close proximity to these genera, but other types were still unknown. Among these isolates, genera Staphylococcus and Bacillus are common to rice endophytes.

PARTENOKARPI: BUAH TANPABIJI - APA, MENGAPADAN BAGAIMANA Sukamto, L Agus
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Fruit growth and development occurred in plant for producing the offspring. Pollination and fertilization will form embryo and seed, which produce and supply plant growth regulators (PGR) for fruit growing. The role of seed can be exchanged with supply of POR exogenously and produce seedless fruit. Seedless fruit could be produced from triploid plant by crossing between tetraploid x diploid plants, in vitro culture of endosperm/irradiated pollen and genetic engineering or treated diploid plant by using PGR, pollen stress, chemical agent/ antibiotic and environment/ plant control. The benefit of triploid plant is quicker growth and produce of seedless fruits directly, whereas diploid plant could produce seedless fruits after fruit induction with special treatment. The other advantages of seedless fruit on certain plants are increased yield, fruit size/ weight,carotene and total sugar contents, decreased harvest period, yield fluctuation and blossom-end rot. Seedless fruit is preferential of consumer and give a higher price than the seeded fruit. Recently, seedless fruits of tomato, grape, citrus, cucumber and watermelon have been produced in commercial industries

PERSEBARAN DAN POLA KEPADATAN MOLUSKA DIHUTAN BAKAU Budiman, Arie
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mangrove molluscs data collected from some mangrove forest in Indonesia (Sumatra, Kalimantan, Java, Mollucas, and Papua) are used in order to understand the mangrove molluscs distribution and pattern of species abundance. The results of the present study strongly suggest three models (or combination of them) of distribution, i.e. (1) molluscs (especially bivalve) only recruit into certain microhabitat, in wich they reach larger densities; (2) certain species of mollusc may recruit widely, but suffer increase mortality in certain microhabitats; and (3) molluscs(especialy for mobile animals, such as many gastropods) may actively move among macrohabitats, increasing local densities in some of those.The correlation between features of habitat and abundance of molluscs which can be described as preference are discussed.

HETEROBLASTIC DEVELOPMENT IN SIX SPECIES OF WILD PIPER: Piper baccatum Blume, Piper firmum Blume, Piper majusculum C.DC, Piper miniatum 1 Blume, Piper crocatum Ruiz & Pav. and Piper retrofractum Vahl. IP, Astuti; Munawaroh, E; Rahayu, EMD; Aprilianti, P; Sumanto, Sumanto
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Heteroblastik pada tanaman adalah adanya bentuk karakter morfologi daun yang sangat berbeda pada fase muda dan fase dewasa,yang terus berlangsung dari fase muda sampai dewasa. Telah dilakukan penelitian tentang perubahan morfologi daun pada tiga jenis sirih (Piper spp.) liar yang tumbuh di kawasan Hutan Konservasi Suaka Margasatwa Maninjau Utara-Selatan, Tanjung Raya,Kabupaten Agam, Provinsi Sumatra Barat dan tiga jenis sirih liar koleksi Pusat Konservasi Tumbuhan-Kebun Raya Bogor-LIPI.Dalam penelitian ini, jumlah individu yang diamati dari setiap jenisnya adalah 5 individu muda dan 5 individu dewasa, dengan masing-masing jenis/spesies 5 kali ulangan; sedangkan jumlah spesimen voucher setiap jenisnya adalah 10 spesimen. Data yang dicatat adalah kondisi habitat (di hutan dan di Kebun raya Bogor); lokasi di mana tumbuhan tersebut ditemukan (tepi sungai, tepi jalan, merambat di batu, pohon atau tempat rambatan lainnya). Data lain yang dicatat adalah ukuran daun (lebar dan panjang ),bentuk daun pada fase muda dan dewasa. Selain itu juga dicatat karakteristik morfologi daun lainnya. Enam jenis sirih liar (Piper baccatum, P. firmum, P. majusculum, P. miniatum, P.porphyrophyllum dan P. retrofractum) dari kawasan hutan konservasi Suakamargasatwa Maninjau Utara-Selatan dan Kebun Raya Bogor, memiliki perubahan morforlogi daun pada fase muda dan fase dewasa yang dikenal dengan sebutan perkembangan heteroblastik.

PRIMER-PRIMER BARU UNTUK MENGAMPLIFIKASI GEN PENGKODE PROTEIN AMPLOP VIRUS DENGUE STRAIN CH53489 Djajanegara, Ira
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Restriction site of BamHI and Sail must be added in order to express the gene encoding envelope protein of dengue virus strain CH53489 (gene E) into expression vector pMAL-p2x. This approach required the PCR technique for amplification as well as restriction sites addition. However, PCR amplification is prone to error due to the process of misincorporation eventhough using Platinum taq polymerase. Therefore, it is important to be concern that there will be no alteration of the gene especially for biopharmaceutical purposes such as recombinant vaccine. This experiment was aimed to design several primers of DenV-M F, D3-1715s, D3-2117s, D3-1911c and DenV-M R for full length sequencing of the amplified products. Primers were designed in silico using Oligo Explorer and tried in vitro to check the ability of the primers to produce fragments. The sequencing results showed that the amplified product suffered from misincorporation during amplification (98.9% homology). However, the 3-D protein structure prediction did not show any major changes in the protein structure. Further analysis of the expressed protein is required to be used for biopharmaceutical purposes.

EGAKAN BAMBU DIKEBUN RAKYAT KOTAMADYA SALATIGA Widjaja, Elizabeth A; Sunaryo, Sunaryo; Hamzah, Hamzah
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Bamboo is one of the important and favorite plants in the villages, however the management of this plant is less interest either from the people or even the government. Because of that there are many bamboo garden has been turned into housing or industrial area, although some people understand that bamboo can be used to prevent soil erosion as well as water conservation. In Central Java, bamboo is mainly used to make furniture, whereas the bamboo handicrafts is only made by the local people for daily uses.The uses of bamboo for handicraft is mainly occurred in Yogyakarta Province, although the bamboo material for this purposes is imported from Central Java. Because of that a study on the bamboo stand in Central Java Province is very important to support the furniture industry which is found also in this neighbouring city. Based on a study done at the Promasan Village, Salatiga, it is found that the bamboo stand occurred in the plot (50 m x 50 m, 5 plots) was 332.96 clum/ha on 2003, whereas the bamboo stands on 2004 decreased till 331.2 clump/ha. The number of clump might decrease due to over harvesting, so many clump was died. The same phenomena was also occurred at the bamboo potency on 2004 which was decreasing compare to 2003 data. The bamboo potency/clump might be increased, but the total production of bamboo culm/ha/year was decreasing sharply compare to the bamboo condition on 2003. This situation can be seen also from the regeneration of bamboo which was decreasing compare the data on 2003 to 2004. The species found in this village was bambu tali (Gigantochloa apus), which was dominated at this village. This potency of this bamboo stands is very high, because the local people used this bamboo for daily use and it used more than other species.However,Gigantochloa atter has also a demand for the bamboo furniture, because of that the potency of this species is also high.Then Dendrocalamus asper which also been used for bamboo furniture. The potency of Bambusa vulgaris is very low in this village, because this species was rarely used.

PENGARUH RADIASI DAN LOKASI TUMBUH TERHADAP PERTUMBUHAN DAN PENYAKIT HAWAR DAUN TALAS kv KETAN Sukamto, L Agus; Saefudin, Saefudin
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Taro (Colocasia esculenta (L.) Schott) is an important tuber crop, grown widely in humid tropics and a source of carbohydrate for many people in Asia, Africa, and the Pacific islands. The leaves and stalks of taro are used as vegetable, as they contain a high protein that is useful for people diet in developing countries. Taro is underutilized plant that has potency as an alternative food.Plant propagation of taro is usually done by using its rhizome or stolon so that the genetic diversity is very limited. In vitro and irradiated techniques can increase its genetic diversity and multiply the plant that has superior quality and quantity.Taro "Ketan" grown tissue culture have been irradiated with gamma rays S Gy and 10 Gy were planted on two different environmental locations.There were many variants as the results of these treatments. Gamma rays and environmental locations caused genotype changes that were stolon number, plant height, corm fresh weight, and leaf blight disease.Gamma rays caused morphological changes that were orientation, undulation, outline of sinus, margin of lamina,and petiole color.

ANALISA VEGETASI HUTAN RIPARIAN DATARAN RENDAH DITEPI SUNGAI NGGENG, TAMAN NASIONAL KAYAN MENTARANG, KALIMANTAN TIMUR Purwaningsih, Purwaningsih
BERITA BIOLOGI Vol 9, No 5 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Study on the riparian forest of Nggeng River bank at Kayan Mentarang National Park was carried out with quadrate method. It would cover species composition and vegetation structure. The results of a floristic inventory of trees with DBH e" 10 cm in a lowland riparian forest of Nggeng river side show that 106 species, consist of 53 genera and 29 families in the plot of 2 hectare sampled. The two leading families in terms of number of species were Dipterocarpaceae and Myrtaceae while according to the total sum of importance values were Dipterocarpaceae and Fabaceae. The common species in two plots were Saraca hulletii, Parashorea parviflora, Dipterocarpus oblongifolius, Castanopsis motleyana, and Dryobalanops lanceolata. The diversity of the river-side were lower than the other forest in Kalimantan and in north is more rich of species than south plot. The forest type on the river side were shown mixed dipterocarp forest, because the forest dominated by dipterocarps species. Forest structure on the sides of Nggeng River were shown there were some emergent trees, they are up to 45 m tall and in diameter >100 cm. Some emergent trees in the location were such as Parashorea parviflora, Dipterocarpus oblongifolius and Shorea spp. Lists on the most 10 leading family trees, 10 leading family saplings, density, frequency and important values of each inventory plants species are provided.

KAJIAN FEKUNDITAS DAN DAYA TETAS TELUR IKAN BETUTU (Oxyeleotris marmorata) PADA WAD AH PEMIJAHAN YANG BERBEDA Karyaningsih, Sri
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Assessment of fecundity and hatching rate of betutu fish - sand goby {Oxyeleotris marmorata) eggs has been done at the station rearing center in Ngrajek, Magelang Regency, Central Java. The fecundity and hatching rate of sand goby eggs can be used as measuring rod of fry. The result showed that the fecundity of rearing in fiber pond between 4.700 to 10.250 eggs with the hatching rate are 41.44% to 85.06%. The rearing in concrete pond between 5.400 tol4.000 eggs with the hatching rate are 43.20% to 90.00%. Statistical test on relationship indicates that no significant for fecundity and hatching rate about the rearing on different case. The relationship between the fecundity with the body length and the body weight is significant. The fecundity and hatching rate was under the influence of ovary weight, diet and hatchery environment.

POTENSI IKAN MUJAIR (Sarotherodon mossambica) SEBAGAI BIOAKUMULATOR PENCEMARAN PESTISIDA PADA LINGKUNGAN PERTANIAN Sani, Yulvian; Indraningsih, Indraningsih
BERITA BIOLOGI Vol 9, No 1 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

An analysis of pesticide contamination in farm sites was conducted to investigate the impacts of organochlorine contamination in environmental matrices leading to pesticide residue in animal products and to identify a bioaccumulator of freshwater (mujair) fish (Sarotherodon mossambica).The observation sites were selected in accordance to the presence of animal farms within the agricultural areas applying pesticides intensively, such as Bandung, Sukabumi and Bogor districts. Samples consisting of water, soils,animal feed, freshwater fish, poultry meats, eggs and weeds were collected from these areas. The study shows that some organochlorines - OC (lindan, endosulfan and DDT metabolites) were detected from all samples. Endosulfan and lindan appeared to be used extensively for horticulture activity (corn, chilly, cassava and tobacco) in these areas, where both pesticides could also be detected either from animal products (meats and eggs) or water, freshwater fish and soils collected from the same areas.The results reveals that there were positive correlation between OC contamination in the environment and residues in animal products. The weed of babadotan (Ageratum conyzoides) was found growing abundantly around the animal farms and was able to accumulate the pesticides. Furthermore, freshwater mujair fish (S. mossambica) appeared to reduce endosulfan contaminantion in water as indicated by an increase pesticide residues in its tissues.

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