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BERITA BIOLOGI

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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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ANALISIS DELIMITASI JENIS PADA Monascus Spp. MENGGUNAKAN SIDIK JARI DNA ARBITRARY PRIMER PCR [Species Delimitation Analysis within Monascus spp. using Arbitrary Primer PCR DNA Fingerprinting] Suharna, Nandang; Julistiono, Heddy
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i2.2928

A species delimitation analysis within Monascus spp. using Arbitrary Primer Polymerase Chain Reaction (AP PCR) DNA fingerprint was carried out. This is one of the methods used for identification and discrimination of bacterial strains within the same species. Its advantages including using single primer, independent of DNA quality, and observing amplicon shared by only some strain. This study analyzed Monascus sp. MM isolate which was originated from a source contaning high level of ethanol and two M. purpureus isolates which isolated from angkak. However, based on ITS region, 99% homology showed the unclear species delimitation. Therefore, this analysis was aimed at clarifying on the identities of Monascus species tested. The result showed DNA polymorphism among three isolates of Monascus that showed species delimitation. This study showed that species delimitation within Monascus isolates used in this analysis could be supported by AP PCR DNA fingerprinting. Therefor we suggested to use this technique or method for phylogenetic study to clarify taxonomic position of Monascus strains.Â

INDUKSI KALUS DAN ORGANOGENESIS KULTUR IN VITRO Dendrobium lineale Rolfe Hoesen, Djadja Siti Hazar; Witjaksono, Witjaksono; Sukamto, LA
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.790

Callus induction and organogenesis were evaluated in the protocorm like bodies (PLB) culture of Dendrobium lineale Rolfe (Orchidaceae). The globular PLB were cultured in Murashige and Skoog (MS) medium with half strength macronutrients QA MS), supplemented with thidiazuron (TDZ 0,1, 0,5 and 1) mg/1 and 2,4 Dichlorophenoxy acetic acid (2,4-D 0.1, 0.5, 1 and 5) mg/1 as treatments. The media containing 1 mg/1 TDZ and (1 and 5) mg/1 2,4-D was the best treatment for callus initiation (100%). The largest diameter of callus was obtained from 1 mg/1 TDZ (average 3.5 cm). Shoot buds regeneration achieved on 1 mg/1 TDZ (average 41.66 %). However roots regeneration was very low (average 6.66 %) obtained from 1 mg/1 2,4-D. The number of adventitious buds produced from the regenerated shoots on media without plant growth regulator

RESPON PERTUMBUHAN TANAMAN KACANG HIJAU VARIETAS WALET TERHADAP PEMBERIAN INOKULAN RfflZOBIUM DAN MIKORIZA DI LAHAN MASAM [Growth Responses of Mungbean var. Walet to the Application of Rhizobium and Mycorrizha Inoculants in Acid Soil] Abdulkadir, Subadri
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1247

The study on the growth response of mungbean Walet variety inoculated by Rhizobium and VA-Mycormiza inoculant carried out done at UPT-BBOK-UPI, Bergen, Lampung, using Competely Randomized Block Design. Five treatments by using Rhizobium, 5 treatments by using mixed Rhizobium and Myco.rrhiza, 1 treatment by using Mycorrhiza. The plant, without inoculated and without fertilized, the plants without inoculated but fertilized by using urea, the plants without inoculated but fertilized by using TSP, and the plants without inoculated but fertilized by using urea and TSP as control. Each treatment has 3 replications. The height and the weight of the plants, the weight of nodules had been weighted after 30 days planting. The result of the research showed that the plants by giving inoculant better growth compare to the plants without inoculated and fertilized. The weight of plants and root is compered which the plants by giving Rhizobium and Mycorrhiza to the plants without inoculated and fertilized was not significant. However, in the height and the wight of nodules of some treatments were significant to the control.

TINJAUAN ULANG (REVIEW) TINJAUAN FISIOLOGIS DOMESTIKASI ULAT SUTERA LIAR Attacus atlas (Lepidoptera: Saturniidae) [A Physiological Review on Domestication of Wild Silkworm A. atlas (Lepidoptera: Saturniidae)] Ekastuti, Damiana Rita
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Attacus atlas are insects producing silk materials having high economic value. It lives from South East Asia, almost all Indonesia until Australia.The demand of silk from Attacus atlas is quite high (10 tons/month). The A. atlas silk is very exclusive, with permanent in variety colors from light to dark brown. At present, to response the high demand, the people get cocoons from nature. This action could reduce result, and the scarcity of the insect. Beside that, rearing was done by placing the larvae on the trees (outdoor rearing). The outdoor rearing resulted in high mortality because of predators or stress. Better rearing is conducted by indoor rearing, that resulted in high survival rate, and high cocoon or egg production. It is because indoor rearing was supported in better environmental condition than outdoor condition that support physiological process eficiently.

ANALISIS PERUBAHAN POLA GENETIK EMPAT GENERASI MANGGIS (Garcinia mangostana L.) BERDASARKAN MARKA ISSR [Analysis of Genetic Pattern Changes among Four Generations of Mangosteen (Garcinia mangostana L.) Based on ISSR Marker] Noorrohmah, Siti; Sobir, Sobir; Efendi, D.
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Mangosteen reproduces trough apomixes mechanism, from which the seed develops without fertilization. Mangosteen is an obligate apomicts and it is believed that all of its progenies may have the same genotype as their mother plant. However it was found that genetic variations occured among mangosteen accessions. This research is aimed to study of genetic changing among generations mangosteen. The plant materials used were four generations (P1, P2, P3, and P4) of mangosteen taken from Wanayasa, Purwakarta.The samples were selected according to tree height and taken from each sector (north, east, south, and west). Genetic observations were conducted using Inter Simple Sequence Repeat (ISSR) technique. Ten primers were used to amplify the genomic DNA of mangosteen. It was found that the genetics pattern differed among tree of different age. More variation was observed on the older mangosteen tree.This information may be useful to determine the optimum age of mangosteen for becoming mother plant.

ADAPTASI AKSESI KENTANG HITAM {Plectranthus rotundifolius (Poir.) Spreng} TERHADAP BERBAGAI INTENSITAS CAHAYA [Adaptation of Black Potatoes Accessions {Plectranthus rotundifolius (Poir.) Spreng} to Different Light Intensity] Utami, Ning Wikan; Wawo, Albert Husein
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Light is one of limiting factors for plant growth, including black potatoes {Plectranthus rotundifolius (Poir.) Spreng}. This species is a potential carbohydrates source that has been utilized in some areas as an alternative food.Plant adaptation to light intensity is controlled by genetic potential and environment.The aim of this research is to obtain information about the light intensity that suitable for growing some black potato accessions and mutants. The study was compiled using Nested Randomized Complete Block Design.Five accessions (Solo, Sangian, Nganjuk, 6G and 25G) planted in 3 degrees of light intensity i.e. full light (N0), 70% (N1), 50% (N2) and 25% (N3).The results revealed that light greatly affect the growth and production of 5 accessions of black potatoes. Five accessions tend to tolerant for decreasing light intensity until 70%. Decreasing in growth and yield characters significantly occurred in 25% of light intensity than control. Solo and Sangian stable to 4 light intensity treatments.

IDENTIFIKASI GALUR Ralstonia solanacearum DENGAN HIBRIDASI SLOT BLOT DNA suryadi, Y
BERITA BIOLOGI Vol 6, No 4 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i4.3447

R. solanacearumÂ (RS), the casual agent of bacterial wilt is one of the most destructive pathogen in the tropical and sub tropical areas that affected various economic crops. Since the pathogen is very diverse, it is necessary to identify the variation of RS isolates. The Polymerase Chain Reaction (PCR) was used in this study to amplify 16S rRNA gane. Two DNA primer pairs namely pr#a andÂ pr#b were used as probe to identify thirtyfour strains of Rs respresenting different biovars isolated from various host. The DNA probes were labelled using digoxygenin and deteced by DNA slot blot hybridization. Resutl showed that the DNA probes colud hybridzed specifically with target DNA, hence distinguish variation of Rs isolates. This assay will be of futher used in the strain identification of Rs from wide range of host from various geographic distribution.

PANEN HUJAN DAN ALIRAN PERMUKAAN UNTUK PENINGKATAN PRODUKTIVITAS PERTANIAN LAHAN KERING, PENANGGULANGAN BANJIR DAN KEKERINGAN Irianto, Gatot
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Rainfall and runoff harvesting study at the Kali Garang basin, Semarang under the cooperation program between Centre for Soil and Agroclimate,Agency of Agriculture Research and Development (CIRAD) France and Government of Central Jawa for improving soil productivity,controlling flood and drought are presented in this paper.The results show that through water harvesting it is possible to increase diversity, improving added value and decreasing agriculture risks of agriculture commodity. Moreover rehabilitation of degraded land and soil conservation practices can be promoted.

RESPON TANAMAN PADI GOGO (Oryza sativa L.) TERHADAP STRESS AIR DAN INOKULASI MIKORISA Sukiman, Harmastini; Adiwirman, Adiwirman; Syamsiyah, Syofiatin
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

It has been known that upland rice production is lower than low land rice production because it is inhibited by soil fertility, water supply and pest.One solutions to increase the production of upland rice is by applying the potential soil microorganism known as mycorrhizae.The objective of this research is to know thef Vesicular Arbuscular Mycorrhizae (VAM) role for upland rice under water stress condition. The research was conducted in greenhouse of Cikabayan, Bogor Agricultural University and Soil Microbiology Laboratory of Research Centre of Biotechnology, Indonesian Institute of Science Centre. The experimental design was factorial randomized block design with two factors. The first factor is inoculation of mycorrhizae consisted of two treatment i.e. without mycorrhizae (MO) and with mycorrhizae (Ml); while the second factor is water stress regime consisted of five treatments namely well watered (SO), water stress on tillering stage (SI), primordial stage (S2), anthesis stage(S3) and grain filling stage(S4). Mycorrhizae significantly increased root infection, phosphate uptake, height, number of tillering, leaf, Leaf Area Index (LAI), productive tillering, filled grain, grain weight and yield. While, water stress significantly decreased number of root infection, shoot wet fresh weight, root dry weight, increased phosphate uptake and sterilized grain, but it didnt significantly decrease yield. The interaction both mycorrhizae and water stress didnt significantly influence all variables, except the root dry weight. The inoculation of mycorrhizae could increase 19.62% on SI, 17.32% on S2, 29.14% on S3, 6.89% on S4 dried harvested yield.

APLIKASI MARKA MOLEKULER PADA BUAH DAN BIJI KOPI ASAL KALIMANTAN TIMUR [Molecular marker application of cherry and green bean of East Kalimantan coffee] Fatimah, Fatimah; Urnemi, Urnemi; Mustopa, Apon Zaenal; Syahrumsyah, Hudaida
BERITA BIOLOGI Vol 13, No 1 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Two quantitative traits, cherry and green bean characters are the important phenotypic selection in coffee breeding practice. The important well-known character from coffee markets is cherry and bean size. In this study, 43 genotypes of coffee were collected from four districts in East Kalimantan i.e. Kutai Kertanegara, Kutai Timur, Berau dan Paser Utara. The objective of this study was to identify cherry and green bean character using quantitative trait locus (QTL) molecular marker, genetic variation from developed alleles, cluster analysis and association analysis of molecular marker, and phenotype observation. Based on polymorphic information content (PIC) of primers used in this study, the genetic variation was low. Based on cluster analysis, two major groups were identified. The first group corresponds to Arabika that consisted of 3 districts, Kutai Timur, Berau and Paser Utara. The second group correspond to Robusta mostly from Kutai Kertanegara.Significant association of primer markers M480 and M312 with QTL has suggested that they can be used as specific primers linked to size of cherry and green bean.Furthermore,they were potential marker assisted breeding in coffee breeding program.

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