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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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SIFAT PROTEKSI EKSTRAK AIR PANAS TEH {Camellia sinensis (L.) Kuntze} HIJAU PADA KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARACETAMOL Julistiono, Heddy
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

In order to develop yeast Candida tropicalis as a model cell for evaluation of substance having anti- or pro-oxidant activity in cell level, the effect of hot water tea {Camellia sinensis (LJ Kuntze) extract on peroxidized lipids, a marker of oxidative stress and cell mortality in the yeast caused by paracetamol has been evaluated. Incubation of yeast cell in the presence of 1.38 % green tea for 2 h decreased cell viability followed with increasing of peroxidized lipids, whereas 0.27 % green tea caused increasing of peroxidized lipids without causing cell death.Yeast cell was not affected by 0.1 % green tea. Incubation of yeast cell with presence of 0.15 % (g/v) paracetamol for 2 h did not cause cell mortality, however content of peroxidized lipids increased significantly. In the presence of higher dose of paracetamol (0.3 %) cell viability remarkably decreased and followed with increasing of peroxidized lipids significantly. Green tea of 0.1 % increased cell viability of cells treated with 0.3 % paracetamol while peroxidised lipids decreased. The data indicated that high dose of paracetamol caused oxidative stress in yeast cells, while green tea with lower concentration might protect paracetamol toxicity due to its antioxidant property. Although the antioxidant property, high dose of green tea could cause oxidation due to its pro-oxidant activity. In conclusion, yeast C. tropicalis could be potentially used as a model cell to evaluate substances having antioxidant property in cell level.

KEANEKARAGAMAN TUMBUHAN PAKU TERESTRIAL DI HUTAN KOTA DKI JAKARTA [Terestrial Ferns Diversity in Urban Forest DKI Jakarta] Andayaningsih, Dwi; Chikmawati, Tatik; Sulistijorini, Sulistijorini
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Urban forest is one of the green open area consisted of natural forest-like vegetation. It has suitable microclimate for understory habitat, including terrestrial ferns. The diversity of fern in three urban forest in DKI Jakarta is reported, i.e the urban forest at The University of Indonesia (UI) Campus, The Cibubur Arboretum and PT JIEP (Jakarta Industrial Estate Pulogadung). Vegetation analysis was conducted by purposive sampling method with a transect line of 200 m in each location with 15 plots of (3x3) m2 each. Fern determination was done based on herbarium specimens and literatures. Fern diversity was analyzed based on Shannon diversity index, and equality index. Canonical correspondence analysis (CCA) was performed to determine the correlation between fern diversity and abiotic factors. There were 18 fern species classified into 11 genera and eight families, at the UI urban forest and Cibubur Arboretum, but there was no fern in PT JIEP. Fern with the highest importance value index was Sphaerostephanos sp. (161.33%) in The UI urban forest, and Lygodium microphyllum (Cav.) R.Br. (71.63%) in Cibubur Arboretum. The CCA result showed that the fern diversity correlated to four abiotic factors i.e temperature,humidity, light intensity and soil pH.

TOKSISITAS GLISEROL ATAU SUKROSA PADA SEL KHAMIR accharomyces cerevisiae YANG DISEMPAN PADA SUHU RENDAH BEKU Julistiono, Heddy
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1123

The effect of cryoprotectants glycerol and sucrose on cell viability and fermentation rate of Saccharomyces cerevisiae after freezing (-30 Â°C) and Chawing (30 Â°C) were studied.Both freezing and thawing were done rapidly. The mortality of cells treated with low concentrations of cryoprotectans (2.5, 5, and 10 %) after 15 and 30 days of cryopreservation, was remarkably higher than that of control and higher concentration (20% and 40%).Glycerol or sucrose with concentration of 20 % and 40 % protected cells from severe mortality only after 90 days of cryopreservation.Fermentation rate of cells treated with 20 % or 40 % of the two cryoprotectants were higher than that of control after 60 and 90 days of cryopreservation.The data indicated that in certain circumstance cryoprotectant could be toxic for the cells during freezing and thawing.Since biomembrane is not permeable to sucrose, therefore we proposed that target of sucrose toxicity may be extracellular, whereas glycerol, which penetrate cells,targets of the toxicity may be both extracellular and intracellular domains.Interaction between cryoprotectant and cell membranes is discussed.

STRUKTUR DAN KOMPOSISI JENIS TUMBUHAN HERBA DAN SEMAI PADA HABITAT SATWA HERBIVOR DISUAKA MARGA S ATWA CIKEPUH,SUKABUMI, JAWABARAT [Structure and Composition of Herbaceous and Seedling Communities on the Herbivore Habitat within Cikepuh Wildlife Sanctuary, Sukabumi, West Java] Sadili, Asep
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The research was conducted to study the structure and composition of herbaceous and seedling communities (understorey species)occurred on herbivore habitat within Cikepuh wildlife sanctuary. A series of 25 plots of 1 m x 1 m each with an interval of 5 m were used to establish a species-area relationship. The proportion (percentage) of canopy (leaf) coverage of each species occurred within the sampling plots to the sampled soil surface area was measured. A total of 54 species belonging to 47 genera and 29 families occurred in the 25 plots develoved, with the species diversity index (value) of 2.09 using the Shannon Wiener formula. Nine species were categorized as herbaceous plants (16.67 %) while the other 45 species were categorized as seedlings (83.33 %). The average density measured was 39 individuals/m or c. 386.400 individuals/ha. The most dominant family is Euphorbiaceae (possessing 5 diffrent species) while the most dominant species is Urochloa subquadripara (Poaceae) with and importance value of 96.08%.

KARAKTERISASI GENETIK IKAN KELABAU (Osteochilus kelabau) DARI BERBAGAI LOKASI DI KALIMANTAN BARAT MENGGUNAKAN METODE RAPD (Random Amplified Polymorphism DNA) Kusmini, Irin Iriana; Gustiano, Rudy; Mulyasari, Mulyasari
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Kelabau fish (Osteochilus sp.) is an endemic fish to Kalimantan inland waters that is potential to be developed. The aim of this research was to characterize Kelabau fish and to study the data base (genetic character) of kelabau as well as its relationship. The result showed that the highest polymorphism and heterozigosity was on Kelabau Sintang and the lowest was on Kelabau Kapuas Hulu. The closest genetic distance value were between Kelabau Pontianak-Kapuas Hulu (0.5351) and the furthest were between Kelabau Pontianak-Sintang (0.6852).

BIODIVERSITAS Basidiomycetes DIKECAMATAN KELILA, KABUPATEN JAYAWIJAYA, PROVINSI PAPUA DAN MANFAATNYA SEBAGAI BAHAN MAKANAN DAN OBAT TRADISIONAL Imamuddin, Hartati; Suliasih, Suliasih
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Biodiversity of Basidiomycetes was studied in Kelila, Jayawijaya, Papua.The aim of this observation was to identify species diversity of Basidiomycetes and their utilities. Sixty one species were found in the location. The 19 species were edible mushroom while 7 species have been used as traditional medicines and some species have a common odours (smell) and 1 species (Clavatia sp.) contains clavatin which could be used as arm tumour agent.

KUALITAS NUTRISI ANEKA TEPUNG DAN KUE TALAM BERBASIS BAHAN PANGAN PULAU ENGGANO DENGAN PENAMBAHAN Lactobacillus plantarum B110 [Nutrition Quality of Various Flour and Talam Cake Based on Enggano Island Food Material Added with Lactobacillus plantarum B110] Khusniati, Tatik; Sulistiani, Sulistiani; Choliq, Abdul; Nanta, Dhea Loka; Wardani, Dita Kusuma; Saraswati, Dahniar
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2222

Nine foodstuffs from Enggano island were processed to flour as wheat flour alternative. To increase flour quality and its derivative product, Lactobacillus-plantarum B110 was added those flour and talam cake made from such flour. They were forest cassava (Dioscorea sp.), ararut sago (Marantha arundinacea), tacca (Tacca Leontopetaloides), egg taro (Alocasia sp.), oil taro (Alocasia sp.), gadung (Dioscorea hispida), gogo rice (Oryza sativa), corn (Zea mays), and belinjo (Gnetum gnemon).Â The quality assessment consisted of HCN detection (qualitative), nutrition contents (proximate analysis), acidÂ (titration method), glucose (GOD-POD kit), and organoleptic tests (20 panelists). The results show that acid and glucose contents of the nine flours increased after L. plantarum B110 addition. Acid content ofÂ the flours was 0.0144-0.2475%, whileÂ glucose wasÂ 0.056-0.449%.Â Carbohydrate, energy and acid contents of L. plantarum B110 talam cake were higher than control, which those were 34.13%, 190.33 cal/100gr, 0.00082%, while protein, lipid, water, ash were 1.75%, 5.22%, 57.9 %, 1.01%, respectively. The talam cake was accepted by panelists with values: 5.50 (taste), 5.6 (colour), 4.55 (flavour), 4.00 (texture), 4.35 (homogeneous). It was concluded that the flour and talam cake quality increased with addition of L. plantarum B110.Â

PERAN PEMUPUKAN POSFOR DALAM PERTUMBUHAN TANAMAN JAGUNG (Zea mays L.) DI TANAH REGOSOL DAN LATOSOL FahmF, Arifin; Syamsudin, Syamsudin; Utami, Sri Nuryani H; Radjagukguk, Bostang
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Phosphorus (P) is an essential element for plants, deficiency and excess of P in soil will limit plant growth. It is required toÂ applicate P fertilizer in appropriate dosage for promoting plant growth on the soil with different properties. The purpose ofÂ experiment was to study the respon of maize (Zea mays L.) for phosphorus fertilization on Regosol and Latosol soils. A glassÂ house experiment was conducted to study maize response on P fertilizer application in Regosol and Latosol soils. The treatmentÂ 1was 0, 112, 336, 1008, 3024 and 9072 kg superfosfat ha . P fertilizer tend to increase the biomass of maize but exceed of PÂ fertilizer reduced maize biomass. The optimum fertilization and vailability of P for plant affected by soil properties.

PATOGENISITAS ISOLAT BAKTERI Xanthomonas oryzae pv. oryzae DAN PEMANTAUAN PENYAKIT HAWAR DAUN BAKTERI PADA PADI GALUR ISOGENIK [Pathogenicity of Xanthomonas oryzae pv. oryzae Isolates and Bacterial Leaf Blight Disease Monitoring on Rice-Near Isogenic Lines (NILs)] Suryadi, Yadi; Kadir, Triny Suryani
BERITA BIOLOGI Vol 16, No 2 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i2.2393

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is an important rice disease due to its high intensity that and Â the mereased durability of its resistant variety. This study was aimed Â to determine the pathogenicity of Xoo isolates, which obtained from four regions/districts in West Java on three rice near isogenic lines (NILs) containing a single resistance (R) gene; and Â to study reaction of rice NILs/ differential genotypes containing mixture of resistant genes to Xoo population under endemic areas. Out of 22 Xoo isolates have been collected and further determined by ELISA assay. Ten Xoo isolates were selected and inoculated to identify their pathogenicity on three NILs i.e., IRBB5 (xa5), IRBB7 (Xa7) and IRBB21 (Xa21). Pathogenicity test showed that most of isolates produced large lesion, and four virulence groups were identified. Phylogenetic analysis suggested that the dominant virulent isolates were widely distributed at several district in West Java. It was shown that 11 NILs exhibited high levels of resistant reaction to the predominant Xoo pathotypes in Cianjur, while 10 lines were susceptible. The single R gene (IRBB7, IRBB21 and Java14) exhibited lower BLB severity. The IRBB50, IRBB51, IRBB52, IRBB53 lines (carrying two R genes), IRBB56, IRBB57 (carrying three R genes); IRBB64 (carrying four R genes), and IRBB66 (carrying five R genes) showed lower severity and thus produced higher resistance to Xoo. This study may implied further work to deploy effective R genes against certain Xoo pathotypes in differ Â region. Â Â

Shorea henryana - JENIS MERANTINON REKALSITRANT Soetisna, Usep; Priadi, Dody
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Shorea henryana - a member species of Dipterocarpaceae known as white meranti. Ecologically distributed on lowland Dipterocarp forest and flourishing in coastal areas. A fairly large tree up to 40 m tall with bole up to 115 cm diameter. Seeds were dispatched from Thailand and showing 72% germination and initial moisture content of 20.3%. First lot of seeds were dried to moisture content 12.5%, 9.4% and 6.1% respectively. Germination of seeds dried to 6.1% m.c. was still relatively high (68%). Contrary to the desiccation resistance, however, the dried seeds seemed not to withstand relatively low temperature for Shorea species, i.e. 14-16 C. Further study will be needed as to investigate a proper storage treatment for this important woody species.

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