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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 39   40   41   42   43 
Karakter Suara Limnonectes modestus (Boulenger, 1882) Asal Suaka Margasatwa Nantu, Gorontalo, Sulawesi Bagian Utara Kurniati, Hellen; Hamidy, Amir
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1315.381 KB) | DOI: 10.14203/jbi.v14i2.3728

Abstract

ABSTRACTCall characteristics of Limnonectes modestus (Boulenger, 1882) which is endemic to Sulawesi have never been described before.  The advertisement calls of two individual males collected from Nantu Wildlife Sanctuary, Gorontalo, northern Sulawesi were recorded in December 6, 2014 at air temperatures of 26°C.  Adobe Audition 3.0 software was used to visualize sound waves.  Advertisement call of L. modestus is very simple, which only has one type of call containing 1-6 pulses.  Pulse duration, pulse rate and call rate of the two individual males are very diverse, ranged between 21-56 milli seconds, 2.58 to 7.87 pulses/second and 0.09-1.16 call/second; but the frequency variation of call is very low, the lower frequency range between 515.6 to 593.0 Hertz (CV=3.1%), whereas the upper frequency range between 2179.0 to 2859.0 Hertz (CV=5.4%); the band width of the frequency range between 1616.5 to 2320.0 Hertz (CV=7.1%). Key words: vocalization, Limnonectes modestus, Nantu Wildlife Sanctuary, North Sulawesi 
Induksi, Multiplikasi dan Pertumbuhan Tunas Ubi Kayu (Manihot esculenta Crantz) Genotipe Ubi Kuning Secara In Vitro Supatmi, Supatmi; Rahman, Nurhamidar; Hartati, N Sri
JURNAL BIOLOGI INDONESIA Vol 14, No 2 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i2.3738

Abstract

ABSTRACTObjectives of the research were to determine the best source of explant origin, the optimum culture media and effects of various concentrations and types of growth regulators on the induction and multiplication as well as the growth of in vitro shoot of cassava Ubi Kuning genotype. The experiment was arranged in a complete randomized design with three factors, i.e the source of explant origin (from field and greenhouse), the growth media containing of 6-Benzyl Amino Purine (BAP) and Gibberellin (GA3) at the concentration level of 1-2 ppm, and multiplication medium supplemented with BAP, Naphtalenic acetic acid (NAA) and Kinetin at concentration level of 0.1 mg/l and 0.5 mg/l. The results showed that the cuttings explants from the field showed more shoots and the average shoot height of axenic culture in the fourth week ranging from 0.2-1.2 cm compared to those from the field (0.5- 1.3 cm). Axenic culture showed no significant growth in growth media (MS) supplemented with BAP and GA3 (1-2 ppm). In the multiplication medium, the highest number of roots were obtained when plantlets were grown in the treatment medium MS + BAP 0.5 mg / l followed by MS + NAA 0.1 mg / l. The largest number of nodes was produced on plantlets grown in MS + BAP media 0.1 mg / l and MS + NAA 0.5 mg / l (p <0.05). While the longest root growth was obtained in plantlets cultured on MS + Kinetin 0.1 mg / l medium. Plantlets showed high survival (100%) after they were acclimatizedfor 4 weeks in a greenhouse using a medium containing a mixture of compost, coco peat, and ground burn husk with a ratio of 1: 1: 1. Keywords: genotype, ubi kayu, ubi kuning  in vitro 
KAJIAN GENETIKA UNTUK KONSERVASI BADAK SUMATERA (DICERORHINUS SUMATRENSIS GLOGER, 1841) Zein, Moch Syamsul Arifin; Fitriana, Yuli Sulistya; Kurniawan, Yuyun; Chaerani, Kurnia; Sirupang, Meriam
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3767

Abstract

ABSTRACTThe Sumatran rhinoceros is one of the most critically endangered species of large mammals due to habitat loss, fragmentation and illegal hunting so that the population of this species drastically decreased. At present, reproductive problems with a limited population are also a threat and require an appropriate solution. Therefore, data on molecular genetic information is very important as a basis for conservation management in maintaining long-term persistence of this species. Phylogenetic analysis based on sequences of CO1, 12SrRNA, and Cytochrome b gen from mitochondrial DNA genomes using neighbor-joining and genetic distance matrix calculations with the Kimura 2-parameter model (K2P) were implemented in pairwise distance calculations in the Mega (Molecular Evolutionary Genetics Analysis) program version 6.05. The study results show the genetic distance of Sumatran Rhinos from Sumatra and Kalimantan respectively 0.2 ± 0.00%, 0.8 ± 0.4%, and 0%. These results were reconfirmed that the Sumatran Rhino species in Sumatra and Kalimantan were taxonomy is no different. The study of genetic diversity based on D-loop of mitochondrial DNA contained 5 haplotypes, namely haplotypes 1 and 2 originating from the island of Sumatra and haplotypes 3, 4, and 5 originating from the island of Borneo. The genetic distance between individuals in this study ranged from 2.54 ± 1.4%, haplotype diversity (Hd) was 0.8 ± 0.172, nucleotide diversity (Pi) was 0.02269, Fu?s Fs value was 2.523, and Tajima?s test was 0.69497. The positive value (Fu?s Fs and Tajima?s test) indicated low genetic diversity and population expansion in the Sumatran rhino. In the study using 10 microsatellite loci, where the average number of allel/loci in Kalimantan (1.68) was higher than in Sumatra (1.22). Data from this study show that genetic variation between Sumatran rhinoceros from Sumatra and Kalimantan can be used as a basis for alternative that the populations of Sumatra and Borneo be considered as a single management unit.  Keywords: Sumateran  Rhinoceros, Mitochondrial DNA, Microsatellite
PERTUMBUHAN VEGETATIF TANAMAN TOMAT (LYCOPERSICUM ESCULENTUM MILL.) DARI BENIH LAMA YANG DIINDUKSI KUAT MEDAN MAGNET 0,1 MT, 0,2 MT, DAN 0,3 MT Novitasari, Vina; Agustrina, Rochmah; Irawan, Bambang; Yulianty, Yulianty
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3816

Abstract

ABSTRACTTomatoes (Lycopersicum esculentum Mill.) horticultural which are very good for consumption as well as industrial materials.  However, the cultivation of tomatoes still faces many obstacles, one of them is the seed.  The quality of old seeds decreases with age of seeds, so that it will affect a crop production.  This study aims to determine whether the magnetic field strength can improve tomato plant vigor.  The study was conducted using a completely randomized design (CRD) of one factor, the induction of a magnetic field consisting of 3 levels, namely 0.1 mT (M0.1), 0.2 mT (M0.2), 0.3 mT (M0,3) for 7 minutes 48 seconds. This study uses two controls;  positive control the new seed (Sn) and negative control was the old seed (So) from not being given a magnetic field treatment each experiment unit is repeated 5 times.  The parameters measured were plant height, chlorophyll content, and carbohydrate content.  The data obtained were analyzed.  If there was a difference between treatments, it was continued with the smallest difference between treatments using the Tukey's test at the 5% level.  The results of the analysis prove that the magnetic field induction of the old seed can increase seed vigor, causing plant height, chlorophyll and carbohydrate content to be the same as plants from new seeds.    Keywords: tomatoes, old seeds, strong magnetic field  
A NEW INDIGENOUS CYANOMETHANE-DEGRADING BACTERIUM ISOLATED FROM GOLD MINING WASTE WATER Sulistinah, Nunik; Munandar, Hendra; Sunarko, Bambang
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3807

Abstract

ABSTRACT The gold mining wastewater effluent is potential source for isolating cyanides-degrading bacteria, since cyanide is commonly used in gold extraction process in the mining industry. An indigenous bacterial strain LP3, capable of growing on and utilizing of a high concentration of cyanomethane (up to 1.0 Molar), could be isolated from Cikotok gold mine effluent. Based on 16S rDNA sequence, the strain was identified as Rhodococcus pyridinivorans. During the growth on cyanomethane (CH3CN), ethanamide (CH3ONH2) and ethanoic acid (CH3COOH) were detected in the growth media, indicating that nitrile hydratase and amidase involved in the metabolism of the substrate. The involvement of both enzymes on the conversion of cyanomethane was also proved by our study on cyanomethane biodegradation using whole cells of R. Pyridinivorans LP3. Besides cyanomethane, the R. pyridinivorans LP3 could also utilize various aliphatic, aromatic, heterocyclic nitriles and amides as growth substrates. Base on these results, R. pyridinivorans LP3 is expected to be used as a potential candidate for biological treatment for cyanide-containing wastes, although further research is still needed, before being applied on a field scale.  Keywords: biocatalyst, cyanide degrading bacteria, gold mining, Rhodococcus pyridinivorans LP3
PEMANFAATAN LIMBAH CAIR SAGU UNTUK MEMPRODUKSI SELULOSA BAKTERI Ahmad, Sitti Wirdhana; Yanti, Nur Arfa; Muhiddin, Nurhayani H.
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3763

Abstract

ABSTRACTBacterial cellulose is an exopolysaccharide produced by bacteria and has a high purity level compared to plant cellulose and has unique structural and mechanical characteristics that can be utilized for various industrial purposes such as food, medical, plastic and paper. This study aims to determine the potential of sago liquid waste as a substrate for producing biocellulose and sugar concentration is required in producing bacterial cellulose from sago liquid waste. Production of bacterial cellulose from sago liquid waste was done with static condition for 14 days with treatment of sugar concentration 5,10,15 and 20% (w/v) using Acetobacter xylinum. Parameters were measured include of thickness, yield, crude fiber content and moisture content. Production of bacterial cellulose using sago liquid waste requires the addition of sugar as much as 10% with a thickness of 21.73 mm, yield of 34.97%, crude fiber of 4.5% and moisture content of 91.35%. Therefore, sago liquid waste is potentially used as a substrate for producing bacterial cellulose.  Keywords :Biocellulose, nata, Acetobacter xylinum, Production substrate  
KAJIAN PENDAHULUAN FLORISTIK DAN FITOSOSIOLOGI POHON DI PULAU SIMEULEU PROVINSI ACEH Sadili, Asep; Royyani, M. F.; Agusta, A.; Afandi, I.; Efendy, O.; Ashari, H.; Keim, A. P.
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3812

Abstract

ABSTRACTSimeuleu island is located in the western part of Sumatera belonging to Aceh Province. Floristic and phytososiological information of vegetation in Simeuleu island is limited and not much study has been conducted, especialy trees species group (diameter ?10 cm). Two  study plots were used: one hectare at Kuala Makmur forest and one ha at Alafan forest (100 m × 100 m). Density analisys at Kuala Makmur recorded 329 individuals/ha while at Alafan consisted of 377 individuals/ha (average of 353 individuals/ha).  Total basal area at Kuala Makmur 29.48 m2/ha while at Alafan  was 44.28 m2/ha (with an average of 36.88 m2/ha). The Shannon-Wiener diversity index (H?) at Kuala Makmur was 2.94 and at Alafan was 2.73 (whit an average H?of 2.84). The total canopy heights of the two study sites as were relatively similar consisting of four strata. Dominant species occurred at Kula Makmur was Shorea atrinervosa (Importance Value, IV=83.55%) and at Alafan was Dipterocarpus grandiflorus (IV=128.06%). Dominant familly at Kuala Makmur was Dipterocarpaceae and at Alafan was Ebenaceae. The distribution of dominant tree species at Kuala Makmur is less prevalent than at Alafan.  Keywords: Simeuleu, Aceh, trees structure, species richness, lowland forest.
PENGELOLAAN KANDUNGAN BAHAN ORGANIK PADA LIMBAH CAIR LABORATORIUM PROLINGMSP-IPB DENGAN BERBAGAI KOMBINASI AGEN BIOREMEDIASI Pratiwi, Niken Tunjung Murti; Hariyadi, Sigid; Ayu, Inna Puspa; Apriadi, Tri; Iswantari, Aliati; Wulandari, Dwi Yuni
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3768

Abstract

ABSTRACT Wastewater from laboratory glassware washing activities contains organic materials. Decomposing bacteria could increase the biomass by utilizing organic matter as a source of carbon. This research was aimed to describe the influence of commercial probiotic application in reducing organic matter content of Proling laboratory wastewater. The research was conducted from February to July 2016 in Aquatic Microbiology Laboratory. The experiment consists of several treatments, those were wastewater (L), wastewater+molase (LM), and wastewater+molase+bacteria from commercial probiotic (LMB). Major parameters observed were COD and bacteria abundance. The results showed that there was significant response difference between molasses and without molasses treatment. Based on COD, the most influenced treatment in decreasing organic matter were LM and LMB. These treatments could decrease COD up to 97% and 91%. Furthermore, the abundance of bacteria in molasses treatment was higher than without molasses. Based on this study, the most effective treatment in reducing organic matter was LMB.  Keywords: bacteria, laboratory wastewater, molasses, organic matter
ESTIMASI POPULASI TOKEK RUMAH, GEKKO GECKO (LINNAEUS, 1758) DI KAKI GUNUNG KARANG, KABUPATEN PANDEGLANG, PROVINSI BANTEN, JAWA BAGIAN BARAT Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3808

Abstract

ABSTRACTOn CoP 18 which will be held in Sri Lanka in 2019, house gecko was proposed to be uplisted up listed from the non-appendix status of CITES to be included the list of CITES appendix II on the reasons that the hunting of the reptile was very large and uncontrolled. In order to anticipate the up listing of house gecko status, LIPI as a Scientific Authority in Indonesia recommended quota for house gecko in 2019 to be 1,800,000 individuals. To see that such a quota does not have a negative impact on the population of house gecko in the wild, a survey was conducted by using the close questioner system method by visiting housing in a village; where in this survey the villages were located at the foot hill of Gunung Karang, Pandeglang Regency, Banten Province. The survey was conducted on March 18 to April 1, 2019. The total number of villages visited was 8 villages which were divided into three sub-districts. The total area surveyed in 8 villages was around 94.5 hectares.  The total number of houses visited was 740 houses; the total number of houses that contained the house gecko was 211 houses. The total number of house gecko by interviewed was 260 individuals, while the total number of house geckos by observed was 88 individuals. The survey results obtained an average population density of house gecko by interviewing eight villages was 3.66 individuals/hectares, while the observation results were 1.15 individuals/hectares. The approach to the average population density of houses gecko by interviewing with the results of 3.66 individuals/hectares was assumed to be the density that was close to the logical number.  Extrapolating the density of house geckos in Pandeglang Regency which covers an area of 229,071 hectares excluded paddy field area was considered to be inhabited by house gecko, so the extrapolation of the number of house geckos in Pandeglang Regency is around 838,399.86 individuals.  Keywords: Gekko gecko, house gecko, Java, population   
EVALUASI KEMUDAHAN TRANSFER MARKA SSR PADI UNTUK MENGANALISIS KERAGAMAN GENETIK FAMILI POACEAE TOLERAN KEKERINGAN Fatimah, Fatimah; Masumah, Masumah; Prasetiyono, Joko; Sustiprijatno, Sustiprijatno
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3764

Abstract

ABSTRACTAnticipating climate change and its impacts on crop production, it requires the development of adaptive rice varieties that respond to drought stress and minimize the damage while keeps growing and maintaining yield. The aim of this study was to evaluate the transferability of rice simple sequence repeat (SSR) markers to Poaceae family and analyze the genetic diversity among 16 accessions of Poaceae family (12 genera and 14 species) using 41 rice SSR markers. The result revealed that the transferability of rice SSR markers was varied. High amplification produced in rice group (>70%) and low amplification in grass group (<30%) with an average of 35.2%. A total of 128 cross-species alleles were identified with an average of 3 alleles/locus. The value of gene diversity ranged from 0.15 to 0.83 with an average of 0.53 and the value of Polymorphic Information Content (PIC) ranged from 0.14 to 0.80 with an average of 0.46. The results of phylogenetic analysis determined two clusters at similarity coefficient of 0.72. The first cluster consisted of 14 accessions from Poaceae family (12 genera and 14 species) while the second cluster consisted of two cultivated rice varieties (Inpari 30 and Situ Bagendit). The genetic relatedness data revealed from this study could be used as basic information for parental selection. The 14 accessions of Poaceae family have a potential for drought tolerant donor and separated clearly from cultivated rice varieties (Inpari 30 and Situ Bagendit) for recipient parents in spike-stalk injection method (SIM) to develop drought tolerant rice varieties.  Keywords: Cross amplification, Drought tolerant, Inpari 30, Situ Bagendit   

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