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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 40   41   42   43   44 
CITRUS HYSTRIX DC DARI JAWA TENGAH DAN SUMBA TIMUR KOLEKSI KEBUN RAYA BOGOR Astuti, Inggit Puji; Ajiningrum, P. S.
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3813

Abstract

ABSTRACTBoth Citrus macroptera Montrouz. and Citrus micrantha Wester were cited as different species from C. hystrix by several authors. Morphological characters that can be used to differentiate those species are leaf shape and fruit. C. hystrix is cultivated at Bogor Botanic Gardens originated from Central Java Province and East Sumba Island, East Nusa Tenggara Province. In previous study, C. hystrix, both from Central Java and East Nusa Tenggara, did not produce flowers as well fruit yet. The only useful characters for differentiation of the species were leaf morphology and its  thorns. Therefore, further study is needed. The research was carried out from January 2013 to December 2018 at Bogor Botanic Gardens and the Anatomy and Cytology Laboratory, Botany Department, Research Center of Biology LIPI. Flower and fruit morphology have been observed on C. hystrix originated from both Central Java and East Nusa Tenggara as well as from other localities which is commonly used. To support this reasearch then leaf and petiole anatomy have been done. The result showed that morphological characters of leaf shape and fruit of C. hystrix where originated from Central Java, differed from  C. hystrix originated from East Nusa Tenggara and those are commonly used by local people. However, based on leaf and petiole anatomy observations of all specimens used on this research are quantitavely similar, so that these results are not supported the morphological characters observation. Therefore, it is assumed that both C. hystrix, originated from Central Java and East Nusa Tenggara, as variety of  C. hystrix that commonly used by local people.
EVALUASI KETAHANAN GALUR HARAPAN KEDELAI TERHADAP PECAH POLONG DAN KERAGAAN KARAKTER AGRONOMI YANG SESUAI UNTUK IKLIM TROPIS Krisnawati, Ayda; Adie, M. Muchlish
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3769

Abstract

ABSTRACT Pod shattering is one of major constraints in soybean cultivation at tropical environment. The objective of the study was to evaluate resistance to pod shattering and agronomic performance of several  adapted to tropical environment. A total of 14 promising lines and two check cultivars (Anjasmoro and Dega 1) were evaluated in two locations (Mojokerto and Jembrana) on Februari to May 2018. The experiment design was a randomized block in each location, with 16 treatments and four replications. At R8 stage, 30 pods were randomly detached from five sample plants of each line to be used for evaluation of pod shattering resistance using oven dry method. Five promising lines from Mojokerto showed resistant to pod shattering, meanwhile three very resistant and two resistant lines were obtained from Jembrana. Those all resistant promising lines were the progenies of shatter-resistant parent (Anjasmoro). The pod shattering resistance was determined by the pod length, the longer the pods will increase sensitivity to shattering. However, the sensitivity of long pods can be minimized when width of the pod is narrow. The promising lines with shorter pod and the width of the pod is narrow, then this line has the chance to be resistant to pod shattering.  The agronomic characters of soybean are suitable to be developed in tropical regions such as Indonesia are: maturity under 85 days, seed weight more than 14 g/100 seeds, medium plant height, and produce high number of pods. Based on the results of the study obtained one promising line which resistance to pod shattering, produce high productivity and have good agronomic characters. The lines is G511H/Anj//Anj///Anj////Anj-4 with production yield 3.43 t/ha and also adaptable to the tropics.   Keywords: pod shattering, agronomic character, tropical soybean, path analysis   
INDUKSI POLIPLOIDI TANAMAN KANGKUNG (IPOMOEA AQUATICA FORSSK.) KULTIVAR SALINA IN VITRO DENGAN ORYZALIN Rahmi, Putri; Witjaksono, Witjaksono; ratnadewi, diah
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3760

Abstract

ABSTRACTWater spinach is a vegetable plant consumed by people all over the world. Its small morphology requires improvement to increase the size and productivity. An alternative way to do that is by increasing the ploidy of its chromosome. This paper describes in vitro induction of polyploidy of water spinach. Inoculum of in vitro seedling, in vitro shoots and nodal stem segments were immersed in MS solution containing oryzalin at concentrations of 0.00, 1.25, 2.50, 3.75 and 5.00 µM with immersion duration of 4, 8 and 24 hours. Oryzalin treatments reduced the growth variables of the in vitro shoots of all inocula tested, compared to control of no oryzalin treatment.  On immersion of 24 hours and high concentration of 5.00 µM, many inoculum failed to grow to the third passage of subculture. The best growth of in vitro shoot to the third passages occurred in the treatment combination of oryzalin 1.25 µM-8 h immersion and 2.50 µM-4 h immersion for seedling inoculum, treatment combination of 3.75 µM-4 h immersion for shoot tip inoculum and 1.25 µM-4 h immersion for nodal stem segment, compared to other treatments. Analysis of flow cytometry on 41 leaf samples from oryzalin treatment derived shoots showed 14.63% tetraploid, 36.59% mixoploid and 48.78% diploid. The efficiency of tetraploid formation reached  60%, provided only by the treatment of oryzalin 1.25 µM-8 h on seedling segments inoculum. Tetraploid shoots need to be proliferated, acclimatized, grown into planting materials and planted for agronomical analysis to provide evidence whether or not tetraploid water spinach is viable for commercial cultivation. 
EVALUASI PERTUMBUHAN STEVIA REBAUDIANA BERT. TETRAPLOID SECARA IN VITRO DAN DI LAPANG UNTUK PRODUKSI STEVIOSIDA DAN REBAUDIOSIDA-A Adabiyah, Rifatul; Ratnadewi, Diah; Ermayanti, Tri Muji
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3809

Abstract

ABSTRACTGenetic improvement through tetraploid induction of Stevia rebaudiana is important in order to increase the sweetener content, steviol glycoside. Tetraploid plants of several species after induction with colchicine and oryzalin have higher growth and secondary metabolite contents compared to the diploid plants. This study was aimed to evaluate growth as well as their stevioside and Reb-A content of S. rebaudiana tetraploid and diploid (control) plants cultured in vitro and grown in the field after acclimation process. This study used 3 tetraploid clones, namely B60.3H8, P1T22, P3T5, and 1 diploid clone as control. Shoot tips were cultured on MS medium without addition of plant growth regulators for 6 weeks, then they were acclimated in a greenhouse, followed by planting them in the field. Growth of shoot culture, plantlets in the greenhouse and plants in the field were observed. Analysis of stevioside and Reb-A was done by HPLC. The results showed that plantlets of diploid clone had higher in vitro growth and survival rate in the greenhouse than that of tetraploids. Tetraploid clone P1T22 had similar growth as diploid plants, but higher than the growth of tetraploid B60.3H8 and P3T5. Fresh and dry weights of B60.3H8 was similar with diploid plants, but higher than P1T22 and P3T5 tetraploid clones. The highest level of stevioside and Rebaudiosida-A was found in tetraploid B60.3H8 clone, the lowest was found in the diploid plants. The highest ratio of stevioside : Reb-A was found at B60.3H8 tetraploid clone.  Keywords: Stevia rebaudiana, in vitro, field, growth, Stevioside, Rebaudioside-A, tetraploid  
VARIASI JUMLAH KROMOSOM TALAS BENTUL (COLOCASIA ESCULENTA (L.) SCHOTT) IN VITRO HASIL PERLAKUAN ORIZALIN Ermayanti, Tri Muji; Rantau, Deritha Ellfy; Wulansari, Aida; Martin, Andri Fadillah; Hafiizh, Erwin Al
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3765

Abstract

ABSTRACTChromosome number analysis is required after polyploid induction with oryzalin. Flowcytometry analysis is a simple and quick method to determine the ploidy level, however, chromosome number analysis is needed in order to confirm variation in the chromosome numbers which has occurred. The aim of the research was to investigate chromosome number variation of polyploid taro (Colocasia esculenta) after in vitro treatment with oryzalin. Nine treated-oryzalin clones and four taro cultivars, as control treatment, were used in this experiment. Ploidy level confirmation was done by flowcytometry analysis, meanwhile chromosome number calculation was performed by squashing method. Roots were isolated from  in vitro plantlets for squashing, leaves were isolated from the same plantlets were used for flowcytometry analysis. At least three plants consisted of 6-52 cells having good chromosome distributions were calculated for their chromosome numbers. The results showed that ploidy level of taro corresponded to the number of chromosomes. Flowcytometry analysis of diploid, triploid, tetraploid as well as hexaploid clones, all has chromosome numbers similar to those as their ploidy levels. Range of the chromosome numbers varied, with most of cells had around their normal chromosome numbers. From 5 to 15% of cells had aneuploid numbers lower or above their normal chromosome numbers.  Keywords : Colocasia esculenta, flowcytometer, polyploid, chromosome number, oryzalin, in vitro  
SCREENING AND EVALUATION OF VARIOUS CARBON SOURCES ON THE ABILITY OF TRICHODERMA HARZIANUM INACC TO SOLUBILIZE INSOLUBLE PHOSPHATE Napitupulu, Toga P.; Ilyas, Muhamad; Kanti, Atit; Sudiana, I. Made
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3814

Abstract

ABSTRACTOur previous works revealed that Trichoderma harzianum InaCC (Indonesian Culture Collection) produced volatile organic compounds that inhibited the growth of Fusarium oxysporum. The objective of this study was to evaluate the ability of T. harzianum InaCC to solubilize insoluble phosphate and optimize the carbon sources condition. Screening was conducted to 10 isolates of T. harzianum on Pikovskaya?s broth in 100-mL conical flasks. The cultures were incubated at 30 ± 1°C for  7 days on a rotary shaker at 80 r/min. All  T. harzianum isolates were observed for the solubility of insoluble tricalcium phosphate with the highest ability was performed by T. harzianum InaCC F88. Using this isolate, the optimization of insoluble phosphate solubilization was conducted on modifed Pivoskaya medium in 500-mL conical flasks in similar screening incubation condition with applying of different combination of phopshate sources (tricalcium phosphate and rock phosphate) and carbon sources (dextrose, lactose, and sucrose). When tricalcium phosphate was substituted by rock phosphate, less soluble phosphate was produced. Glucose was the best carbon source used for solubilization of both tricalcium phosphate and rock phosphate, then followed by lactose and sucrose. Acidification of medium was not the major mechanism of phosphate solubilization by T. harzianum. Based on the ability of high phosphate solubilization, T. harzianum InaCC F88 is the most potential strain as a plant growth promoting fungus among the 10  isolates of T. harzianum Ina CC.  Key words:  biocontrol, Pikovskaya medium,  phosphate solubilization, Trichoderma harzianum
BUAH MERAH (PANDANUS CONOIDEUS LAMK) BIORESOURCES PEGUNUNGAN TENGAH PAPUA: KEANEKARAGAMAN DAN UPAYA KONSERVASINYA Wawo, Albert Husein; Lestari, Peni; Setyowati, Ninik
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3770

Abstract

ABSTRACT The red fruit (Pandanus conoideus Lamk) is one of the local bioresources on the Central Highlands Region of Papua. Exploration to reveal the red fruit diversity in this region had been carried out in 2016 and 2017 which covers Kurima, Kurulu, Siepkosi, Wesaput and Kaninmbaga / Bokondini areas. The method used in exploration were interview the local residents, observations of plant morphology, soil, micro-climate, surrounding species, and collection of propagation material. The observed red fruit morphology includes; stem height and diameter, leaf length and width, and number of roots. Observation of fruit development was carried out at the Royal Biological Garden in Wamena (KRBW). The results of the exploration obtained 23 red fruit cultivars in the Central Highlands region of Papua. All cultivars grow in moist environments; the texture of the soil is clay mixed with sand and loam. The development of fruit from young fruit into ripe fruit takes 3-4 months which is divided into 3 stages. Ex-situ red fruit conservation efforts have been carried out at the Royal Biological Garden in Wamena (KRBW). Until now, 141 numbers of red fruits have been conserved in KRBW. The community has also cultivated a number of red fruit cultivars in their yard and garden such as Bergum, Maler, Wona and Wesi cultivars as in situ conservation. The four cultivars are very popular because the fruit is large and the oil content is more than other cultivars.  Keywords: Diversity, Red Fruit, Conservation, Royal Biological Garden in Wamena, Central Highlands Region of Papua  
PUSAT PENELITIAN BIOTEKNOLOGI-LIPI, JL. RAYA BOGOR KM. 46, CIBINONG, 16911, JAWA BARAT, INDONESIA Susetio, Muhammad; Efendi, Darda; Sari, Laela
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3761

Abstract

ABSTRACTBentul taro (Colocasia esculenta) is one of taro cultivar which has high productivity. Genetic improvement has been done by obtaining tetraploid taro. Drought tolerant cultivars can be achieved by addition of poly ethylene glycol (PEG) in vitro. The purpose of this study was to determine the effect of the treatment of PEG concentrations on in vitro growth of tetraploid shoots and to propagate shoots after PEG treatment. The explants used were diploid taro shoots, tetraploid clones 2.1.2 and 2.4.2. Shoots were planted in MS media without PEG and with PEG of 5, 10 and 15%. The growth parameters observed were petiole length, number of leaves, and number of roots every week for 6 weeks. Proline content and LC50 values were analyzed at 6 weeks after planting. Propagation of shoots after PEG treatment was carried out on MS media containing 2 mg / L BAP, 1 mg / L thiamine, and 2 mg / L adenine. Each treatment consisted of 15 shoots as replicates. The growth parameters observed were petiole length, number of leaves, and number of shoots every week for 6 weeks. The results showed that Bentul taro clones significantly affected the concentration of PEG on growth parameters. LC50 value of diploid clone was 12.16%, clone 2.1.2 was 13.54%, and clone 2.4.2 was 12.74%. The highest proline content was found at Bentul tetraploid clone 2.1.2. After PEG treatment, growth was significantly affected by PEG concentrations. All Bentul taro clones after PEG treatment produced multiple shoots.  Keywords: Taro (Colocasia esculenta), in vitro selection, diploid, tetraploid, proline, LC50, propagation  
DINAMIKA POPULASI DAN KERUSAKAN POHON TEMPAT BERTENGGER KALONG (PTEROPUS VAMPYRUS) DI KEBUN RAYA BOGOR Soegiharto, Sri; Kartono, Agus P.; Maryanto, Ibnu
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3810

Abstract

ABSTRACTThe study on population dynamic and  roosting trees damage by flying fox was conducted for 16 months (March 2008-June 2009) in Bogor Botanical Garden.  Recording of individual flying fox was carried out by use direct observation in the roosting trees habitat at 9.00-15.00, with one replication every week. The result indicated that in 2008 the total flying fox were 420 individuals and in 2009 were 563 individuals (160 juveniles, 218 adult females and 185 adult males). The natality and mortality rate in this location is 0.0214 and 0.0099 respectively. The flying fox population growth can be predicted using exponential equation is N(t+1)=563(1.8395)t.  The study also indicated that in the KRB there are 9 individual trees which are the main perch, 2 individual trees of Pterodotus alata, Pterocarpus indicus, and Shorea leprosula, and each 1 individual tree of the species  Dipterocarpus cornutus, Ficus drupacea, and Syzygium syzygioides. The extent of damage to the tree where the perch is very dependent on the diameter of the canopy, bat population size in one tree, and time periods per roosting season.  Keywords: Population Dynamic, Flying Fox (Pteropus vampyrus), Bogor Botanical Garden
PENGARUH PENAMBAHAN BUAH-BUAHAN DALAM PAKAN TERHADAP PERUBAHAN WARNA RAMBUT PADA OPOSUM LAYANG (PETAURUS BREVICEPS) JENIS CLASSIC GRAY Farida, Wartika Rosa; Sari, Andri Permata; Handayani, Tri Hadi; Inayah, Nurul; Nugroho, Herjuno Ari
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3766

Abstract

ABSTRACTThis study aims to determine the possibility of changes in the hair color on young sugar gliders (Petaurus breviceps) were given additional fruits in the feed. Research has been conducted in the Small Mammal Captive Breeding of Zoology Division, Research Center for Biology - LIPI, Cibinong, Bogor. Descriptive method used in this study with 10 days of preliminary and 90 days (3 months) of data collection period. The research material used is 16 young sugar gliders aged about 2-3 months, divided into 2 groups namely group I consists of 8 sugar gliders (4 male and 4 female) whose the parent is brownish brown color, while group II consists of 8 sugar gliders (4 male and 4 females) whose the parent are gray in color. Each group received 2 feeding porridge formulations T0 (control) and T1 (T0 + kepok banana (Musa balbisiana), watermelon (Citrullus lanatus), and red dragon fruit (Hylocereus polyrhizus)). T0 feed composition consists of brown rice porridge, boiled chicken eggs, honey, calcium, and water. Sugar gliders is given live superworm (Zophobas morio) every Tuesday and every Thursday is given live crickets (Gryllidae). During the observation each pair  of young sugar glider is placed in a wire enclosure (60 cm x 42 cm x 42 cm) equipped with feeding box, drinking water bottle and a hammock. The results showed that the average of dry matter intake T1> T0. KII / T0 / enclosure 6 show higher of body weight gain and feed efficiency than KI / T1 / enclosure 4. The addition of fruits into the T1 diet did not show any discoloration in hair on sugar gliders in captivity.  Keywords: fruits, feed intake, hair/fur color, Petaurus breviceps

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