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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 41   42   43   44   45 
PENGARUH PLATELET-RICH PLASMA (PRP) TERHADAP PROLIFERASI DAN VIABILITAS HUMAN DERMAL FIBROBLAST (HDF) DALAM KONSENTRASI GLUKOSA TINGGI Hadi, Restu Syamsul; Kusumah, Indra; Sandra, Yurika
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3815

Abstract

ABSTRACTThe administration of Platelet Rich Plasma (PRP) is expected to be a supplement for treatment of diabetic wounds and hyperglycemia by increasing growth factors. The purpose of this study was to examine the effect of Platelet Rich Plasma (PRP) on the proliferation and viability of human dermal fibroblast (HDF) in high glucose conditions, as a model for healing diabetic wounds in vitro. HDF cells are grown in DMEM medium containing high glucose which are then with PRP. To measure the effect of PRP on the HDF cell proliferation, CCK-8 kit was used and evaluated by using a microplate reader. To evaluate the viability of HDF, an automated cell counter. The results of the research showed that PRP stimulate the HDF cells proliferation. The optimal dose of PRP to increase HDF cell proliferation is at dose of PRP 10%.  Supplementation of PRP is significantly increased cell viability and HDF cell counts within 48 hours. The results showed PDGF growth factor secreted by PRP is increased significantly. The conclusion is PRP stimulated HDF cell proliferation and viability in a high glucose condition. This finding support the used of PRP as a therapy for diabetic wounds.  Keywords: proliferation, viability, human dermal fibroblast, platelet-rich plasma, diabetic
BIOMASA MANGROVE DAN BIOTA ASOSIASI DI KAWASAN PESISIR KOTA BONTANG Yonvitner, Yonvitner; Wahyudin, Yudi; Mujio, Mujio; Trihandoyo, Arif
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3771

Abstract

ABSTRACTMangrove is an important buffer ecosystem in the coastal area which has a function as carbon absorption through biomass growth.  Others also as support any kind ecosystem particularly is fish's biodiversity.  This research that was conducted in Bontang coast found 4 species of mangrove that dominated by Rhizophora apiculate that biomass production average 3714 kg/ha, Rhizophora mucronata 2415 kg/ha, Sonneratia 407 kg/ ha, and Bruguiera 251 kg/ha. Mangrove ecosystem support to biomass production of the shell, shrimp, mollusc until 2408 kg/ha of total population found.  The research found that have a similar trend of mangrove growth and biomass related biota production.  The message of this research is important of mangrove conservation to support biota and another ecosystem in the coastal area.    Keywords:  Mangrove, Association, Bontang, 
AKTIVITAS ANTIBAKTERI DAN ANTIOKSIDAN PEPTIDA SUSU KAMBING HASIL HIDROLISIS DENGAN PROTEASE LACTOBACILLUS PLANTARUM S31 Herlina, Nina; Mustopa, Apon Zaenal; Surachma, Rahma Sari; Triratna, Lita; Kartina, Gina; Alfisyahrin, Wida Nurul
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3762

Abstract

ABSTRACTResearch on the hydrolysis of milk with various protease obtained from the digestive tract, plants and animals has been widely carried out, but the hydrolysis process of goat milk with protease enzymes from Lactobacillus plantarum S31 isolated from bekasam is still widely unknown. This study aims to determine the antibacterial and antioxidant activity of peptides hydrolyzed by protease enzymes from L. plantarum S31. Isolation and purification of extracellular proteases was carried out with 80% ammonium sulfate saturation, dialysis and G50 Sephadex matrix. The results of hydrolysis showed that fraction 11 has the highest antioxidant activity. Goat milk with pH 5 has a greater inhibitory activity of about 29%, which is 5% compared to goat milk pH 7. This fraction also has a quite good antibacterial activity of Entero Phatogenic Escherichia coli (EPEC K1.1) bacteria, Staphylococcus aureus and Listeria monocytogenes.  Keywords: goat milk, hydrolysis, antibacterial, antioxidant, Lactobacillus plantarum
PERTUMBUHAN DAN PRODUKSI TANAMAN JEWAWUT [SETARIA ITALICA (L.) P. BEAUV.] PADA PERLAKUAN DOSIS RADIASI DENGAN PENGURANGAN FREKUENSI PENYIRAMAN UNTUK SELEKSI TOLERAN KEKERINGAN Hidayati, Nuril; Syarif, Fauzia
JURNAL BIOLOGI INDONESIA Vol 15, No 2 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i2.3811

Abstract

ABSTRACTFoxtail millet [Setaria italica (L.) Beauv.] is one of potential cereal plants that can be developed as a source of carbohydrates in marginal and dry lands. The purpose of this study was to examine the tolerance to drought of foxtail millet accession of Buru Merah as a result of gamma radiation treatment. In this study, the seeds of the Buru Merah accession were treated with gamma ray at doses of 0, 25, 50, 100 and 200 Gy. The established growing plants were then treated with different intervals of watering, which were every day (as control), 2 days, 4 days, and 6 days which were arranged in factorial randomized block design with three replications. The observed variables were increase plant height, increase of leaf number, leaf chlorophyll content, plant water potential and  yields. The results revealed that the radiation treatment did not show any significant difference in the production of plant biomass. With radiation treatments of 0, 25, 50 and 100 Gy. and a two-day watering interval the plants can still maintain its water potential above -3 MPa and showing optimum growth. However radiation treatment resulted a significant difference in panicle production, especially between the radiation dose of 200 Gy. and others. The combination of 0-100 Gy. radiation treatment and 2-day watering interval produced plants with optimum panicle production.Keywords: drought, growth, millet, production, radiation, tolerant
KONSUMSI DAN EFISIENSI PAKAN PADA KUKANG (NYCTICEBUS COUCANG) DI PENANGKARAN Wirdateti, Wirdateti; Puspitasari, Dewi; Diapari, Didid; Tjakradidjaja, Anita S
JURNAL BIOLOGI INDONESIA Vol 3, No 3 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i3.3474

Abstract

ABSTRACTFeed Intake and Efficiency of Slow Loris (Nycticebus coucang) in Captivity. Study on feed intake and efficiency of captive slow loris (Nycticebus coucang) has been conducted at the Division of Zoolgy, Research Center for Biology ? LIPI for five weeks. Three individuals were placed in three cages, with its sizes of 1.2 x 1.7 x 1.9 m (one cage) and 0.8 x 1.7 x 1.9 m (two cages). Feeds consisting of papaya, banana, coconut, passion fruit, guava, sweet corn, quail egg, and white bread, were given ad libitum. Average body weight of slow loris in the beginning of the study was 587 gram/head and at the end was 648.33 gram/head. Average of feed consumption was 317.26 gram/kgBW/day. Average of feed efficiency and protein efficiency ratio (PER) was 0.0270 and 0.0945, respectively.Key words: Feed, slow loris, Nycticebus coucang, captivity, feed efficiency, PER.
ANALISIS MOLEKULER PIRAMIDA GEN XA PADA PROGENI PADI VARIETAS CIHERANG DAN INPARI 13 ., Fatimah; Prasetiyono, Joko; Priyatno, Tri Puji; Yunus, Muhammad; Suhartini, Tintin; Ridwan, Iman; Baroya, Mushlihatun
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2161

Abstract

Bacterial leaf blight (BLB) is a major disease in Indonesian lowland rice.This research was undertaken to pyramid three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 into Ciherang and Inpari 13 varieties. The donor parent Code (Xa4+Xa7) was crossed with Angke (Xa4+xa5) while Ciherang and Inpari 13 were crossed with IRBB21 (Xa21). Progenies were selected using marker assisted selection and yield component observation. Foreground selection was conducted using SSR and STS markers linked with the targeted genes in the F1 and DCF1 population. Individuals with triple positives Xa genes were screened for the presence of Xa4 gene as the background. Selected heterozygote plants in F1 Code x Angke, F1 Ciherang x IRBB21 and F1 Inpari 13 x IRBB21 were used to develop DCF1 population. Molecular analysis on DCF1 population through alleles of three BLB resistant genes xa5, Xa7 and Xa21 and one background BLB resistant gene Xa4 resulted 8 (2,6%) in DCF1 Ciherang and 13 (3,5%) in DCF1 Inpari 13. Yield component characters on F1 Code x Angke resulted significant in number of panicle. F1 Ciherang x IRBB21, F1 Inpari 13 x IRBB21 and DCF1 Ciherang resulted significant in weight of empty grain while DCF1 Inpari 13 resulted no significance in all of observed characters. Keywords: Rice, F1 Population, DCF1 Population, molecular marker, Xa gene 
MORFOLOGI LARVA DAN POLA INFEKSI FALCAUSTRA KUTCHERI BURSEY ET.AL., 2000 (NEMATODA : COSMOCERCOIDEA: KATHALANIIDAE) PADA LEUCOCEPHALON YUWONOI (MCCORD ET.AL., 1995) DI SULAWESI TENGAH, INDONESIA Purwaningsih, Endang; Riyanto, Awal
JURNAL BIOLOGI INDONESIA Vol 7, No 1 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i1.3127

Abstract

ABSTRACTMorphological Study of Larvae and Infection Pattern of Falcaustra kutcheri Bursey et.al.,2000 in Leucocephalon yuwonoi Mc Cord et.al., 1995 from Sulawesi. Some nematode parasitesFalcaustra kutcheri Bursey et.al., 2000 were found in alimentary tract of Leucocephalonyuwonoi, those were large in numbers in rectum and a small numbers in stomach. Both adultand larvae were collected from the host. The larvae were different on mouth structure and theshape of esophagus. An additional character, i.e two lateral branches of the anterior part ofintestine, that appear along the growth of larvae were reported.Key words: Nematode, Falcaustra kutcheri, infection pattern, larvae, morphology,Leucocephalon yuwonoi, Sulawesi
SUPEROXIDE DISMUTASE ACTIVITY AND ETHANOL RESPIRATION IN A FUNGI RESISTANCE TO ETHANOL MONASCUS SP. MM Julistiono, Heddy; Suharna, Nandang; Desnora, Beni
JURNAL BIOLOGI INDONESIA Vol 3, No 4 (2002): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v3i4.3319

Abstract

ABSTRACTMonascus sp. MM was a contaminant fungus isolated from museum specimenpreserved with ethanol 70 %. In order to verify role of superoxide dismutase (SOD) inprotecting cell from ethanol toxicity during ethanol metabolism, SOD activities of Monascussp.MM and a Monmcus sp. NGK, which was isolated fiom fermented red rice (angkak), werecompared. When fungus was grown with glucose, CuIZn-SOD activity of Monascus sp., MMwas 7.1 times of thht of Monascus sp. NGK. Whereas in ethanol medium, CuIZn-SOD activityof Monarcus sp. MM was 24.6 times of that in Monascus sp. NGK. Induction of CuIZn-SODMonmcus sp. MM by ethanol was not observed. Compared with Mn-SOD, activity of CuIZn-SOD was markedly important (I 0 times of Mn-SOD when fungi grown with ethanol; 12 timeswhen the fungi grown with glucose). The data indicated that Cu/Zn-SOD might play animportant role in protecting cell fiom ethanol toxicity during ethanol metabolism. Ethanolrespiration rate of Monascus sp. MM was also important since O2 consumption and ethanoldegradation rates were clearly higher than that of Monascus sp. NGK.Keywords: Monarcw sp., superoxide dismutase, respiration, ethanol resistance.
SENYAWA ANTIBAKTERI DAUN BELAWAN PUTIH (TRISTANIOPSIS WHITEANA) Handayani, Dewi; S. Achmadi, Suminar; Agusta, Andria
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.334

Abstract

Isolation of antibacterial compounds from chloroform extract of belawan putih (Tristaniopsis whiteana) leaves hasbeen done. The bioautography analysis of the extract against Escherichia coli and Staphylococcus epidermidis,subsequently followed by isolation and purification of active compounds led us to obtain two pure compounds,compound 1 and 2. Futhermore, the compound 2 was identified as betulinic acid based on their spectroscopic dataincluding ultraviolet spectrum, infrared spectrum, 1D- and 2D-NMR spectra. In addition, this is the first report ofthe occurrence of this compound in the plant of Tristaniopsis whiteana and the genus of Tristaniopsis.Keywords: antibacterial, active compound, leaves, Tristaniopsis whiteana
ADAPTASI EKOFISIOLOGI TERHADAP IKLIM TROPIS KERING: STUDI ANATOMI DAUN SEPULUH JENIS TUMBUHAN BERKAYU Rindyastuti, Ridesti; Hapsari, Lia
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3089

Abstract

ABSTRACTStudy on foliar anatomic structure of tropical plants are useful to study various aspects of plant life related to photosynthetic, productivity, carbon sequestration, adaptation to environmental stresses as the results of global climate change, etc. This study was conducted to investigate foliar anatomic structure of ten tropical woody plant species including Barringtonia asiatica, Dracontomelon dao, Heritiera littoralis, Diospyros discolor, Calophyllum inophyllum, Antidesma bunius, Schleichera oleosa, Syzygium cumini, Madhuca longifolia dan Adenanthera pavonina; also to discuss its implication to ecophysiological adaptation to tropical dry climate. The results showed that ten woody plant species studied had variation of adaptation pattern to dry tropical climate, exposure to high light intensity or open shade and photosynthesis activity. Plant species in Group II (Antidesma bunius, Madhuca longifolia and Adenanthera pavonina) has relatively higher adaptation compared to Group I (Diospyros discolor, Calophylum inophyllum, Dracontomelon dao, Schleichera oleosa, Heritiera littoralis, Baringtonia asiatica and Syzygium cumini). Some foliar anatomic structures including palisade shape and size, stomatal index, stomatal density, spongy mesophyll thickness, the ratio of P/T and P/S are important characters that can be used as indicator of changes in environmental factors; particularly the density of stomata as indicators of climate change in dry tropic area.Keywords: adaptation, dry climate, eco-physiology, foliar anatomy, woody plant

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