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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 58   59   60   61   62 
INDUKSI POLIPLOIDI PADA TANAMAN TALAS (COLOCASIA ESCULENTA (L.) SCHOTT) KULTIVAR KALIURANG DENGAN PERLAKUAN KOLKISIN SECARA IN VITRO Ermayanti, Tri Muji; Wijayanta, Ardian Nur; Ratnadewi, Diah
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3667

Abstract

ABSTRACTGenetic modification to increase productivity and other better growth characteristics of Kaliurang taro could be done by various methods; one of them is polyploid induction. Kaliurang taro has performed excellent traits, it is resistant to leaf blight disease and pests. Polyploid plants of Kaliurang taro are expected to have excellent char-acteristics and increased productivity. The objective to this study was to obtain an efficient method through in vitro induction of polyploidy using colchicine on Kaliurang taro. Aseptic plantlets of Kaliurang taro were soaked in colchicine solution at 0.05, 0.1 and 0.2% for 1, 2 and 3 days. Control was untreated plantlets. Each treatment consisted of 12 replicates. The colchicine-treated plantlets were then planted on MS medium containing 2 mg/L BAP, 1 mg/L thiamine and 2 mg/L adenine. Survival rate and vegetative growth of plantlets were observed every week for 8 weeks after planting. The results showed that increasing colchicine concentra-tion and the soaking period produced planlets with various survival rate. Flowcytometric analysis indicated that the treatment of soaking the plantlets in 0.05% colchicine for 1 day resulted in 14.3% of tetraploid plantlets. The most efficient of colchicine treatment was 0.2% for 3 days, resulting in 57.1% tetraploids, with the efficient value of 33.3%. There was chromosome multiplication from diploid to tetraploid which was confirmed through both flowcytometric analysis and chromosomes counting.Keywords: Taro (Colocasia esculenta L.), Kaliurang, in vitro, flowcytometer, chromosome multiplication, tetraploids
UJI BAKTERI SIMBIOTIK DAN NONSIMBIOTIK PELARUTAN CA VS. P DAN EFEK INOKULASI BAKTERI PADA ANAKAN TURI (SESBANIA GRANDIFLORA L. PERS.) Widawati, Sri
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2204

Abstract

ABSTRACTNitrogen-fixing bacteria that dissolve phosphate and produce IAA (plant growth hormones) are useful for biological organic fertilizer (BOF). Thirty nitrogen-fixing isolates have been isolated from the rhizosphere soil on Mount Salak, Bogor. This study aims to find nitrogen-fixing symbiotic and non-symbiotic bacteria that could potentially dissolve phosphorus (P) and produce hormones IAA. The N-fixing bacteria were tested to promote growth of turi (Sesbania grandiflora) during  seedling. Turi is called astringency tree, because the entire tree is useful for land reclamation, produce tannin, gum, resin, vegetables, medicine, tonic, antipyretic, and as pulp material. Pikovskaya medium was used to evaluate the ability of nitrogen-fixing bacteria to dissolve phosphate. The effect of bacteria on the growth of seedlings Sesbania grandiflora were evaluated using pot experiment. Pot contained sterile sand were arranged as factorial with 3 replications and watered with sterile distilled water containing Muller solution in the green house. The results showed that, the highest populations of bacteria, P solubilization index, and PMEase produced by Rhizobium, Azotobacter and Azospirillum were isolated from the rhizosphere of tea plant. The isolates were identified as Azotobacter chrococcum and Azospirillum lipoferum and Rhizobium sp. All of the isolates produced plant growth hormon (IAA). Effects of inoculants ABC (Azospirillum, Azotobacter, Rhizobium 1), ABD (Azospirillum, Azotobacter, Rhizobium 2), BCD (Azotobacter, Rhizobium 1, Rhizobium 2), dan ABCD (Azospirillum, Azotobacter, Rhizobium 1, Rhizobium 2), were very good on growth of Turi plant seedlings. All isolates formed root nodules, except the ABD and D isolats. Keywords: Nitrogen fixing bacteria, Phosphate solubilizing bacteria, Sesbania grandiflora L.Pers. 
KERAGAMAN GENETIK AMFIBIA KODOK (RANA NICOBARIENSIS) DI ECOLOGY PARK, CIBINONG BERDASARKAN SEKUEN DNA DARI MITOKONDRIA D-LOOP Astuti, Dwi; Kurniati, Hellen
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3147

Abstract

ABSTRACTGenetic Diversity of Amphibia (Rana nicobariensis) at Ecology Park, Cibinong Based onDNA Sequences of Mitochomndrial d-Loop. The 397- base pairs from ten nucleotide sequencesof mitochondrial d-loop region were determined and analyzed in object to study the geneticdiversity of frog Rana nicobariensis at Ecology Park, Cibinong, West Java. There were sixhaplotypes from 10 individuals collected from Ecology Park. Haplotype and nucleotide oramino acid diversities in Ecology Park were 0.964 and 0.0064 respectively.Key words: Genetic diversity, Rana nicobariensis, Ecology Park Cibinong, DNA sequences,mitochondrial-d-loop
INDIKATOR KUNCI PEMULIHAN FUNGSI HABITAT BURUNG DI LAHAN REKLAMASI DAN REVEGETASI PASCA TAMBANG BATUBARA Soegiharto, Sri; Zuhud, Ervizal A.M.; Setiadi, Yadi; Masyud, Burhanuddin
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3404

Abstract

ABSTRACTThe area of reclamation and revegetation in farmer coal mine has one role as bird habitat. The purpose of this study was to determine indicators of the types of bird that can progress successed reclamation and revegetation program in habitat function of fauna. The method used was bird species and bottom vegetation identification I in each habitat type used transect and mistnet. The data was analyzed using multivariate analysis of hDCCA. The results using hDCCA preedicted that the key indicator of bird characteristics that marks the development of succession in the fifth year is the presence of ?insectivore bird? type. Bird species were B15 (Lanius schach), B20 (Prinia flaviventris), B23 (Picoides moluccensis), B28 (Pachychepala hypoxantha), B29 (Rhipidura javanica), B30 (Orthotomus ruficeps), B31 (Orthotomus sericeus).Keywords: hDCCA, insectivore bird, key indicator, reclamation and revegetation,
PERFORMA PERTUMBUHAN KUKANG JAWA (NYCTICEBUS JAVANICUS E. GEOFFROY,1812) YANG DIBERI TAMBAHAN BISKUIT DAN MONKEY CHOW DALAM PAKAN Farida, Wartika Rosa; Astuti, Wulansih Dwi; Sari, Andri Permata
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2109

Abstract

This study aims to determine the effect of the addition of biscuits and monkey chow in the feed to the growthperformance of java slow loris (Nycticebus javanicus E. Geoffroy, 1812). Research has been conducted in CaptivitySmall Mammals, Zoology Division, Research Center for Biology - LIPI for 52 days consisted of a 10 days period offeed adaptation and 42 days (6 weeks) period of data collection. The material used is six java slow loris aged 1 - 1,5years consisted of three males and three females. Alternative feed given during the study were banana (Musa sp.),papaya (Carica papaya), fuji apple (Malus domestica), pear (Pyrus amygdaliformis), guava (Psidium guajava),cucumber (Cucumis sativus), sweet potato (Ipomea batatas), boiled quail eggs, biscuits sprinkled with sugar, monkeychow, crickets, and mealworm. Parameters measured were feed intake, body weight gain, feed efficiency, and totaldigestible nutrients (TDN). The data were analyzed using analysis of variance, followed by Duncan's multiplerange test. The results showed the average of dry matter intake in PII (45.89 g / head / day) was significantly higher(P <0.05) than that in PI (43.19 g / head / day) and P0 (42,17g / head / day. The average of body weight gain ofslow loris in PI> PII> P0, namely respectively 3.49 g / head / day, 3.41 g / head / day, and 3.15 g / head / day,while the feed efficiency in the treatment PI> P0> PII, respectively 8.20%, 7.47%, and 7.44%. The average oftotal digestible nutrients (TDN) of slow loris in PI (96.33%) was significantly higher (P <0.05) than that in PII(92.91%) and P0 (85.50%). The data of nutrient consumption can be used as a basis for the preparation of rationsand feed for slow loris in captivity.Key words: growth, feed efficiency, Nycticebus javanicus, captive breeding
VIRUS INFLUENZA NOVEL H1N1 BABI DI INDONESIA Dharmayanti, NLP Indi; Ratnawati, Atik; Hewajuli, Dyah Ayu
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3115

Abstract

ABSTRACTNovel H1N1 influenza virus in Swine in Indonesia. Novel H1N1 influenza virus occurred sinceApril 2009 has caused mortality in human population. In Indonesia, this situation requireintensive surveillance to prevent reassortant probability between the H5N1 virus and novelH1N1 virus. This study conduct preliminary surveillance of novel H1N1 virus circulation byusing Real Time-Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR), that validatedby CDC to detect novel H1N1 virus. Result of this study revealed that the influenza novelH1N1 virus was detected in swine/pigs in Indonesia especially in Bulan island and two individualsample from Kapok slaughter house in Jakarta. These findings showed that in Indonesia thenovel H1N1 virus is not only found in human but also has circulated in swine in Indonesia.Key words: pig/swine, influenza novel H1N1 virus
PARASITOID CHALCIDOIDEA (INSECTA: HYMENOPTERA) SEBAGAI AGEN PENGENDALIAN HAMA SECARA BIOLOGI PADA TERONG (SOLANUM MELONGENA L.) DAN PARE (MOMORDICA CHARANTIA L.) Sofyan, Muhamad Rofik; Ubaidillah, Rosichon
JURNAL BIOLOGI INDONESIA Vol 5, No 1 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i1.3206

Abstract

ABSTRACTThe Parasitics wasp superfamily Chalcidoidea (Insecta : Hymenoptera) potentialas biological control agents for pests of Eggplant (Solanum melongena) and BitterMelon (Momordica charantia). The recent contribution on diversity of parasitics waspChalcidoidea for biological control perspective against the pests of the two economiccrops was discussed, with emphasis on the most significant steps for the selection ofcandidate biological control agents. The survey was carried out in Bogor, West Javausing Malaise traps, Yellow pan traps and a Swept net. The crops are the most economicallyimportant in agriculture not only in West Java but also in Indonesia. Meanwhile the pestcontrol of those two crops has so far been used only by the insecticides and would affectto the health of the production of the crops when the vegetables are consumed freshly. Ofthe 78 species within 8 familes of Chalcidoidea are found in both crops, 7 species can beclassified as potential biological control agents. With the object of biological control ofthese 7 species, a survey was carried out to establish the presence and importance oftheir natural enemies on those crops in Java.Key words : Bitter Melon, Chalcidoidea, Eggplant, Parasitoids, Pest control.
EVALUASI KEMUDAHAN TRANSFER MARKA SSR PADI UNTUK MENGANALISIS KERAGAMAN GENETIK FAMILI POACEAE TOLERAN KEKERINGAN Fatimah, Fatimah; Masumah, Masumah; Prasetiyono, Joko; Sustiprijatno, Sustiprijatno
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3764

Abstract

ABSTRACTAnticipating climate change and its impacts on crop production, it requires the development of adaptive rice varieties that respond to drought stress and minimize the damage while keeps growing and maintaining yield. The aim of this study was to evaluate the transferability of rice simple sequence repeat (SSR) markers to Poaceae family and analyze the genetic diversity among 16 accessions of Poaceae family (12 genera and 14 species) using 41 rice SSR markers. The result revealed that the transferability of rice SSR markers was varied. High amplification produced in rice group (>70%) and low amplification in grass group (<30%) with an average of 35.2%. A total of 128 cross-species alleles were identified with an average of 3 alleles/locus. The value of gene diversity ranged from 0.15 to 0.83 with an average of 0.53 and the value of Polymorphic Information Content (PIC) ranged from 0.14 to 0.80 with an average of 0.46. The results of phylogenetic analysis determined two clusters at similarity coefficient of 0.72. The first cluster consisted of 14 accessions from Poaceae family (12 genera and 14 species) while the second cluster consisted of two cultivated rice varieties (Inpari 30 and Situ Bagendit). The genetic relatedness data revealed from this study could be used as basic information for parental selection. The 14 accessions of Poaceae family have a potential for drought tolerant donor and separated clearly from cultivated rice varieties (Inpari 30 and Situ Bagendit) for recipient parents in spike-stalk injection method (SIM) to develop drought tolerant rice varieties.  Keywords: Cross amplification, Drought tolerant, Inpari 30, Situ Bagendit   
RESPON TITER ANTIBODI DAN PROTEKSI VIRUS NEWCASTLE DISEASE GENOTYPE I, II, VI DAN VII SEBAGAI VAKSIN TERHADAP INFEKSI ISOLAT VIRUS NEWCASTLE DISEASE CHICKEN/INDONESIA/GTT/11. Indriani, Risa; Dharmayanti, NLP Indi
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2887

Abstract

ABSTRACTThe Newcastle Disease (ND) virus causes the most important disease in chicken, and very contagiuos in poultry in many countries. In Indonesia the Newcastle Disease or is called tetelo became endemic in chicken currently, the ND virus of genotype VII caused morbidity 100% and mortality 80% in chicken. The fusion and haemaglutinin-neuraminidase protein are involved in adhesion to the surface of hert cells to antibodies. HN and F protein induced by the ND vaccines have neutralizing effects. The aim of this study was to compare the antibody level and protection of several ND vaccine genotypes (genotype I, II, VI and VII) in chicken against ND virus chicken / Indonesia/GTT/11 genotype VII. Our result showed that all genotype ND vaccines (I, II, VI dan VII) produce 100% protection in chicken from clinical and mortality, but the challenge virus excretion in chickens vaccinated with ND vaccine VII genotypes were significantly different (p <0.05) to other groups. Response of antibody in chicken vaccinated ND genotype VII show highest mean titer (GMT 122) compered other ND vaccine. In onclusion, the ND virus chicken/Indonesia/ GTT/11 genotype VII is good to be used as inactive vaccine seed, which appropriate to the field virus circulating at this time.Keywords: virus, Newcastle Disease, genotype, vaccine
ANALISIS VEGETASI HUTAN PAMAH DI PULAU BATANTA, RAJA AMPAT, PAPUA Mirmanto, Edi
JURNAL BIOLOGI INDONESIA Vol 6, No 1 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i1.3173

Abstract

ABSTRACTVegetation Analysis of Lowland Forest in Batanta Island, Raja Ampat, Papua. A vegetationanalysis of Batanta lowland forest has been made by setting up 17 plots of each 30-m x 30-mdistributed in 3 study sites were Yenanas (5 plots), Yensawai (7 plots) and Wailebet (5 plots).All trees (dbh. e?10 cm) within all of 17 plots were measured, and determined their positions,and identify their species. In total there were 171 tree species recorded within plots andbelonging to 108 genera and 40 families. Pometia pinnata was the most common speciesfollowed by Anthocephalus macrophyllus, Pangium edule, Toxotrophis illicifolius, andKoordersiodendron pinnatum. Almost all of common species such as Pometia pinnata,Anthocephalus macrophyllus, Celtis hidebrandii and Intsia bijuga were observed as theemergent and/or canopy trees. According to ordination analysis there were five communitytypes, Aporusa?Pometia, Antocephalus-Toxotrophis, Sterculia-Grewia, Ficus-Antocephalus,and Duabanga-Pterocymbium communities. However floristic compositions varied amongplot sites, which might be a characteristic of vegetation of Papua and the nearby small islands.Keywords: Vegetation, lowland forest, Raja Ampat, PapuaKata kunci: Vegetasi, hutan dataran rendah, Raja ampat, Papua

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