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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 72   73   74   75   76 
BUAH MERAH (PANDANUS CONOIDEUS LAMK) BIORESOURCES PEGUNUNGAN TENGAH PAPUA: KEANEKARAGAMAN DAN UPAYA KONSERVASINYA Wawo, Albert Husein; Lestari, Peni; Setyowati, Ninik
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3770

Abstract

ABSTRACT The red fruit (Pandanus conoideus Lamk) is one of the local bioresources on the Central Highlands Region of Papua. Exploration to reveal the red fruit diversity in this region had been carried out in 2016 and 2017 which covers Kurima, Kurulu, Siepkosi, Wesaput and Kaninmbaga / Bokondini areas. The method used in exploration were interview the local residents, observations of plant morphology, soil, micro-climate, surrounding species, and collection of propagation material. The observed red fruit morphology includes; stem height and diameter, leaf length and width, and number of roots. Observation of fruit development was carried out at the Royal Biological Garden in Wamena (KRBW). The results of the exploration obtained 23 red fruit cultivars in the Central Highlands region of Papua. All cultivars grow in moist environments; the texture of the soil is clay mixed with sand and loam. The development of fruit from young fruit into ripe fruit takes 3-4 months which is divided into 3 stages. Ex-situ red fruit conservation efforts have been carried out at the Royal Biological Garden in Wamena (KRBW). Until now, 141 numbers of red fruits have been conserved in KRBW. The community has also cultivated a number of red fruit cultivars in their yard and garden such as Bergum, Maler, Wona and Wesi cultivars as in situ conservation. The four cultivars are very popular because the fruit is large and the oil content is more than other cultivars.  Keywords: Diversity, Red Fruit, Conservation, Royal Biological Garden in Wamena, Central Highlands Region of Papua  
KOMUNITAS FLORISTIK DAN SUKSESI VEGETASI SETELAH ERUPSI 2010 DI GUNUNG MERAPI JAWA TENGAH Afrianto, Whisnu Febry; Hikmat, Agus; Widyatmoko, Didik
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2895

Abstract

ABSTRACTSuccession dynamics (as a complex interaction and relationship between environment and species) is a crucial ecological process for managing and restoring habitats and ecosystems. This research was conducted at several locations based on the damage levels occurred due to the 2010 eruption of Mount Merapi: heavily damaged site was chosen at the Cangkringan Resort (consisting of both open and covered areas) and the Kemalang Resort, medium damage site was located at the Dukun Resort, and minor damage/relatively intact site was situated at the Selo Resort. A total of 135 plant species belonging to 64 families were recorded from all locations during the study. The Mount Merapi eruption occurred in 2010 had significantly impacted on the floristic community structure and condition. Canonical Correspondence Analysis (CCA) was used to analyze the relationships between environmental variables and the existing plant species conditions. The results indicated that different abiotic environment conditions (variables) significantly influenced species compositions and conditions. The covered area (?= 0:49; p= 0.002; F= 10:35) and the elevation factor (?= 0:32; p= 0.002; F= 7:08) provided the highest impact on vegetation conditions. Meanwhile, the relationships and correlations between edaphic factors and floristic community conditions varied from site to site.Key words: Abiotic environment, floristic community, succession, Mount Merapi, restoration
KELIMPAHAN DAN DIVERSITAS AKTINOMISETES TANAH TAMAN NASIONAL GUNUNG CIREMAI JAWA BARAT Nurkanto, Arif
JURNAL BIOLOGI INDONESIA Vol 5, No 3 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i3.3179

Abstract

ABSTRACTThe Abundance and Diversity of Soil Actinomycetes from Ciremai National Park West Java.Actinomycetes of soil samples from Mount Ciremai National Park, West Java were been isolatedby Sodium Dodecyl Sulphate Yeast Extract (SDS YE) method in Humic Acid Vitamin Agar(HVA) medium. The colonies of actinomycetes were counted based on Total Plate Count(TPC). Diversity of actinomycetes were identified based on spore, chain spore, pigmentation,hypha and aerial hypha formation. The abundance of actinomycetes were 3,50 x104 ? 71,50 x104CFU/g soil. Seven genus of actinomycetes were found, they were Streptomyces, Nocardia,Micromonospora, Microbiospora, Actinomadura, Actinoplanes, and Microtetraspora. Mostof them were distributed in 900 until 2500 m height above sea level. Streptomyces only knownin 2700, 2900 and 3057 m above sea level. Population of Streptomyces were predominant in allsoil types according to height, but were highest in 2500 m asl.Key words: Actinomycetes, fungi, Mount Ciremai, diversity, abundance.
PENGARUH SUHU, PH, ENZIM DAN SURFAKTAN TERHADAP PLANTARISIN F REKOMBINAN ENKAPSULASI SEBAGAI ANTIBAKTERI STAPHYLOCOCCUS AUREUS DAN SALMONELLA TYPHI Mustopa, Apon Zaenal; Hasim, Hasim; Amelia, Suci
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3664

Abstract

ABSTRACTStaphylococcus aureus causes diarrhea, which is one of the main cause of infant mortality, where asSalmonella typhi causes typhoid fever with the incidence rate of 180/100,000/year. Plantarisin F is anantimicrobial peptide that can inhibit the growth of S. aureus and S. typhi. The aim of this study is to determinethe effect of temperature, pH, enzymes, and surfactants of encapsulated F recombinant plantarisin. Plantarisin F(1.61%) encapsulated with maltodextrin (5.36%) and skim milk (2.68%) using the spray dry with inlettemperature 150ºC produced particles that are generally spherical with a rough texture, range in size, yield25.03%, and had good antibacterial activity against S. aureus and S. typhi. The antibakterial activity plantarisinF encapsulated is not affected by the treatment temperature (40ºC-100ºC), pH (2-12), enzyme (proteinase-K,catalase, lysozyme, pepsin, trypsin), dan surfaktan (SDS, urea, triton X-100, PMSF, EDTA) treatment.Keywords: Antibacterial, Encapsulation, Plantarisin F, Salmonella typhi, Staphylococcus aureus
KAJIAN ASPEK EKOLOGIS DAN DAYA DUKUNG PERAIRAN SITU CILALA Pratiwi, Niken T.M.; Hariyadi, Sigid; Ayu, Inna Puspa; Iswantari, Aliati; MZ, Novita; Apriadi, Tri
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2201

Abstract

ABSTRACTEcological aspect (morphometry, a complexity of community structure, trophic state, and carrying capacity) are one of basic information to build comprehensive management concept of aquatic ecosystem, as Lake Cilala.  The concept was addressed to develop ornamental fish floating cage and natural fishery activity.  Lake Cilala is a longwise shape small lake with maximum length, average width, and average depth as 1141 m, 161 m, 2.67 m respectively.  Lake Cilala has various genera of phytoplankton and zooplankton, 8 genera of benthos, 5 genera of aquatic plants, and 12 fish species including planktivore, herbivore, and carnivore fishes.  Those organisms established food chains, by grazing and detritus food chain.  The trophic state of Lake Cilala was categorized as middle to heavy eutrophic level by Trophic State Index (TSI).  The carrying capacity analysis of Lake Cilala is 130 tons/year. It is resulted a possibility to increase ornamental fish floating cage to 165 units from 300 existed units. Furthermore, the carrying capacity for natural fish was reached 3.77 tons/year. Keywords: carrying capacity, food chain, lake fisheries, small lake management, trophic state 
PERBANDINGAN TIGA METODE TRANSFORMASI AGROBACTERIUM UNTUK PENCARIAN GEN-GEN TERKAIT TOLERANSI KEKERINGAN MENGGUNAKAN TRANSPOSON AC/DS PADA PADI CV. BATUTEGI Mulyaningsih, E. S.; Aswidinnoor, H.; Sopandie, D.; Ouwerkerk, P.B. F.; Nugroho, S.; Loedin, I.H. Salmet
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3144

Abstract

ABSTRACTTransformation Strategy for Indonesian Indica Rice in Attempt to Discover Drought-TolerantRelated Genes Using of Transposon Ac/Ds. Attempt to identify, isolate the gene, and study forgene function for several agronomical traits have been done including some drought toleranttraits. Japonica rice cultivars have been used due to its higher efficiencies compared withindica cultivars. Two plasmids namely pNU400 and pUR224 were used to generate mutants ofthese cultivars (Batutegi dan Kasalath cultivars). Those plasmids contain an element calledActivator (Ac) and Dissociator (Ds) respectively. The pNU400 contains GFP (green flourescensprotein) as a selectable marker, whereas the pUR224 contains hygromycine resistant gene andgusA as a reporter gene. Each plasmid was transformed into rice genome of Batutegi andKasalath cultivars by Agrobacterium mediated transformation using three methods oftransformation (A, B and C). The transformation method A was not suitable for both cultivars,where none of plantlets were produced from pNU400 and pUR224 plasmids. The transformationmethod B produced some plantlets from the Kasalath cultivar only using pUR224 plasmid.The transformation method C was the best method to produce transgenic plants from bothcultivars (Batutegi and Kasalath), using both plasmids (pNU400 and pUR224). The PCR analysisshowed that 19 and 9 plants of Batutegi and Kasalath contained both gusA and hpt genesrespectively. None of those plants contained of gusA gene. Southern blot analysis revealed 3independent lines from Batutegi dan 7 independent lines from Kasalath. The integration of Actransposon was analyzed based on expression gfp gene when observed under UV dark reader.This research has proved that indica rice cultivars, especially the Batutegi cultivar of Indonesianorigin, could be transformed. The cultivar could be used as plant model for the indicatransformation.Key words: transformation, drought tolerant, indica rice, Ac/Ds transposons, Agrobacterium.
INDUKSI TETRAPLOID PADA TANAMAN JAMBU BIJI MERAH (PSIDIUM GUAJAVA L.) SECARA IN VITRO Handayani, Tri; Witjaksono, Witjaksono; Nugraheni, K. Utami
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3401

Abstract

ABSTRACTSome commercial varieties of Guava seedless are triploid and rarely found naturally. A triploid variety with less seeds and better yield potential can be achieved by crossing tetraploid to diploid variety. Guava tetraploid plants can be synthetically induced by using oryzalin or colchicine to double the chromosome from its diploid. This research was aimed to obtain tetraploid lines by studying the effects of oryzalin in germination and in vitro growth of Guava. Seed from Red Guava were cultured in liquid medium MS + 2 mgL-1 BA with adding oryzalin according to the treatment, then seed planted in MS solid medium. Seed explants were exposed to Oryzalin 0 (controls), 15, 30 dan 60 ?M with exposure time are 23, 36 and 49 days or 3, 5, and 7 weeks. Results from polyploid induction were 8 tetraploid (5.48%) and 9 (6.16%) mixoploid shoots in vitro. High est tetraploid shoots were obtained from treatments by exposing the seed explant to 15 ? 30 ?M oryzalin for 23 ? 36 days. Oryzalin treatments inhibit germination and in vitro growth of Guava until 1st subculture. After second subculture, tetraploid or mixoploid shoots quantitatively showed no difference respons on in vitro growth with its diploid.Keywords: guava, tetraploid, seed explant, in vitro germination, polyploid
RAPID ASSESSMENT ON BIODIVERSITY IN LOGGED FOREST OF TESSO NILO, RIAU PROVINCE, SUMATRA Prawiradilaga, Dewi M.; ., Purwaningsih; Susiarti, Siti; Sidik, Irvan; Suyanto, A.; Rachmatika, Ike; Noerdjito, Woro A.; Marakarmah, A.; Sinaga, M. H.; Cholik, E.; Saim, A.
JURNAL BIOLOGI INDONESIA Vol 10, No 2 (2014): Jurnal Biologi Indonesia
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i2.2106

Abstract

Tesso Nilo area which is located at Riau province covers an area of 188.00 ha. Recently, it is famous because of asharply increased in encroachment activities for forest conversion, especially for oil palm plantations and villagesites. It was conducted in logged forest around Segati river, Toro river, Mamahan river and Sawan river in June2003. The results showed that the area had the high richness of plant species which was indicated by the high valueof Mennhenick index. Records from the 1 ha studied plot identified a total of 360 species included 165 genera and57 families with 215 tree species 305 sapling species. Some important tree species which were included in the RedList of IUCN were â??gaharuâ?? (Aquilaria malaccensis), â??raminâ?? (Gonystylus bancanus), Shorea spp. and Dipterocarpusspp. The local community utilized 83 species of medicinal plants and 4 species of toxic plants for fishing. The totalnumber of recorded bird species was 114 species represented 29% of the total Sumatran bird species. The mostimportant bird species were the Wrinkled Hornbill (Aceros corrugatus), Crestless Fireback (Lophuraerythrophthalma), Crested Fireback (Lophura ignita) and Hooked-billed Bulbul (Setornis criniger). The total numberof recorded mammal species was 34 species or 16.5% of 206 species of Sumatran mammals. The most importantmammal species included Sumatran tiger (Panthera tigris sumatrae), Sumatran elephant (Elephas maximussumatrensis), the Sun bear (Helarctos malayanus) and three species of primates: Hylobates agilis, Presbytis femoralisand Macaca nemestrina. The herpetofauna contained 15 reptile species and 18 amphibian species. The mostimportant herpetofauna was the endangered False Gharial (Tomistoma schlegelii) and the vulnerable Common Softshelledturtle (Amyda cartilaginea). The number of recorded fish species was 50 represented 18% of the totalSumatran fish species (272 species). The important fish species were Breinsteinea sp. and Chaca bankanensis whichwere unique and rare. Since insects are the largest group of animal, this study only focused on beetles. Theidentified beetles were classified into 644 species and 34 families. The important beetles were the Longhorn beetles(Cerambycidae) and the Scarab beetles (Scarabaeidae).The small mammal parasites consisted of ectoparasites whichwere categorized into 14 species and endoparasites which were categorized into 2 orders and 3 species.Keywords: biodiversity, logged forest, richness, Sumatran tiger, Sumatran elephant
SHOOT TIP CULTURE OF NEPENTHES ALBOMARGINATA LOBB EX LINDL. IN VITRO Sukamto, Lazarus Agus; Mujiono, Mujiono; Djukri, Djukri; Henuhili, Victoria
JURNAL BIOLOGI INDONESIA Vol 7, No 2 (2011): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v7i2.3112

Abstract

ABSTRAKKultur Pucuk Tanaman Nepenthes albomarginata Lobb ex Lindl. secara In Vitro. N. albomarginata adalah kantong semar kerah putih (white collared pitcher plant), salah satu tanaman pemakan serangga yang sangat menarik sebagai tanaman hias. Tanaman ini terancam punah karena pengambilan dan kerusakan habitatnya. Penelitian perbanyakan secara in vitrodilakukan dengan menggunakan pucuk tanaman N. albomarginata pada media formulasi setengah Murashige and Skoog (1/2 MS) dengan tambahan zat pengatur tumbuh 6-benzyladenine (BA) 1 mg l-1 dengan atau tanpa kombinasi dengan á-naphthalene acetic acid (NAA) atau 4 amino 3,5,6, trichloropicolinic acid (Picloram) 0.5, 1, 1.5, and 2 mg l-1. Perlakuankombinasi BA 1 mg l-1 dengan NAA 0.5 mg l-1 menghasilkan pertambahan tinggi tanaman terbesar. Tanaman menghasilkan jumlah daun terbanyak pada kontrol. Perlakuan BA 1 mg l-1 menumbuhkan tunas aksilar terbanyak, sedangkan kombinasinya dengan NAA 1.5 mg l-1 merupakan perlakuan yang dapat menghasilkan tunas adventif. Kombinasi BA 1 mg l-1 dan NAA 2 mg l-1 menginduksi kalus terbaik. Tanaman yang dihasilkan belum membentuk akar, tetapi pertumbuhan lebih lanjut dapat membentuk perakaran dan dapat hidup di luar botol kultur setelah diaklimasi.Kata kunci: Nepenthes albomarginata, tanaman pemakan serangga, kantong semar, kultur pucuk tunas, in vitro
INDUCTION OF SUPEROXIDE DISMUTASE ACTIVITIES AND ETHANOL RESISTANCE BY SODIUM CHLORIDE AND LEAD ACETATE IN YEAST CANDIDA SP. Y390 Julistiono, Heddy
JURNAL BIOLOGI INDONESIA Vol 4, No 1 (2006): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i1.3271

Abstract

ABSTRAKAktivitas Induksi Superoksida Dismutase dan Resistensi Etanol oleh Sodium Kloridadan Plumbum Asetat pada Khamir Candida sp. Y390. Untuk mengetahui sifat khamirCandida sp. Y390 dalam melindungi diri dari cekaman oksidatif, telah dilakukan risetkajian toleransi sel terhadap etanol dan hubungannya dengan enzim superoksida dismutase(SOD) pada khamir tersebut. Viabilitas sel yang ditumbuhkan pada media gliserol dandiinkubasi pada etanol 17,s % selama 1 jam adalah 0.30 f 0.09 % , pada media gliseroldan 1 1,7 % natrium khlorida adalah 1.65 f 0.5 % sedang pada media giserol dan 1 ppmplumbum asetat adalah 1.16 f 0.7 %. Aktivitas CuZnSOD pada sel yang diinduksi olehnatrium khlorida atau plumbum asetat turun sedikit, yakni masing-masing 1,7 dan 1,9kali. Aktivitas MnSOD pada sel yang diperlakukan dengan natrium khlorida naik sedikit(sekitar 1,03 kali), namun pada sel yang diperlakukan dengan plumbum asetat naik secaramenyolok (sekitar 3,6 kali). Data ini menunjukkan bahwa metabolisme etanol dapatmenyebabkan Candida sp. Y390 mengalami cekaman oksidatif. MnSOD khamir mungkinberperan dalam melindungi sel dari kerusakan akibat cekaman oksidatif.Keywords: Candida sp. Y390, yeast, ethanol metabolism, oxidative stress, SOD.

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