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Dental Journal (Majalah Kedokteran Gigi)

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Published by Universitas Airlangga

ISSN : 19783728 EISSN : 24429740 DOI : -

Core Subject : Health,

Dentistry

The Dental Journal (Majalah Kedokteran Gigi) (e-ISSN:2442-9740; p-ISSN:1978-3728) is published by the Faculty of Dental Medicine, Universitas Airlangga. Its diciplinary focus is dental science and dental hygiene. The Dental Journal (Majalah Kedokteran Gigi) is published in English on a quarterly basis with each 50-60 page edition containing between nine and eleven scientific articles on research, study literature and case studies. Contributors to the Dental Journal (Majalah Kedokteran Gigi) included: dental researchers, dental practitioners, lecturers, and students drawn from Indonesia and a wide range of other countries.

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Articles 964 Documents

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Antioxidant potency of mangosteen peel extract topical application in reversing reduced orthodontic brackets tensile strength after bleaching Ananto Ali Alhasyimi
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 4 (2017): December 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Nowadays, cosmetic dentistry has become an ever-increasing requirement with interest in it growing over time. Bleaching is one of the popular cosmetic treatments that has been proven to diminish the tensile bond strength (TBS) of orthodontic brackets attached to bleached teeth. Mangosteen peel (MP) extract contains antioxidants that may potentially reverse the reduction in TBS. Purpose: The purpose of this study was to evaluate the effect of MP extract on the post- bleaching TBS of brackets. Methods: The reported research constitutes an experimental in vitro study conducted on a total of 120 maxillary first premolar teeth randomly divided into six groups (n = 20) as follows: negative-control (NC: no bleaching), positive-control (PC: bleaching + no treatment), and the treatment groups (bleaching + 10% sodium ascorbate (SA), 10% (MP10), 20% (MP20) and 40% (MP40) MP extract gel). Post-treatment, the brackets were bonded using Transbond XT and TBS testing was performed using a Universal Testing Machine. The ARI was examined by means of a stereoscopic microscope, while enamel morphological changes were observed through a Scanning Electron Microscope. The TBS-generated data was analyzed by means of Anova and Tukey tests. For the Adhesive Remnant Index, a Kruskal-Wallis analysis test was performed. Results: There was a significant TBS difference (P = 0.001) between the various groups. The PC group showed the significantly highest TBS compared to the others (8.33 ± 3.92 MPa), whereas NC demonstrated the lowest (4.15 ± 2.27 Mpa). The TBS value of the MP40 group was considerably higher than other groups treated with antioxidants (7.87 ± 3.26 MPa). The failure of orthodontic brackets using MP extract mostly occurred at the adhesive-bracket interfaces. Conclusion: Topical application of 40% mangosteen peel (MP) extract as an antioxidant after bleaching was effective in reversing the reduced post-bleaching tensile bond strength (TBS) of orthodontic brackets.

Differences of Streptococcus mutans adhesion between artificial mouth systems: a dinamic and static methods Aryan Morita; H. Dedy Kusuma Yulianto; Susmira Delta Kusdina; Nunuk Purwanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 2 (2016): June 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Various materials have been used for treating dental caries. Dental caries is a disease that attacks hard tissues of the teeth. The initial phase of caries is a formation of bacterial biofilm, called as dental plaque. Dental restorative materials are expected for preventing secondary caries formation initiated by dental plaque. Initial bacterial adhesion is assumed to be an important stage of dental plaque formation. Bacteria that recognize the receptor for binding to the pellicle on tooth surface are known as initial bacterial colonies. One of the bacteria that plays a role in the early stage of dental plaque formation is Streptococcus mutans (S. mutans). Artificial mouth system (AMS) used in bacterial biofilm research on the oral cavity provides the real condition of oral cavity and continous and intermittent supply of nutrients for bacteria. Purpose: This study aimed to compare the profile of S. mutans bacterial adhesion as the primary etiologic agent for dental caries between using static method and using artificial mouth system, a dinamic. method (AMS). Method: The study was conducted at Faculty of Dentistry and Integrated Research and testing laboratory (LPPT) in Universitas Gadjah Mada from April to August 2015. Composite resin was used as the subject of this research. Twelve composite resins with a diameter of 5 mm and a width of 2 mm were divided into two groups, namely group using static method and group using dynamic method. Static method was performed by submerging the samples into a 100µl suspension of 1.5 x 108 CFU/ml S. mutans and 200µl BHI broth. Meanwhile AMS method was carried out by placing the samples at the AMS tube drained with 20 drops/minute of bacterial suspension and sterile aquadest. After 72 hours, five samples from each group were calculated for their biofilm mass using 1% crystal violet and read by a spectrofotometer with a wavelength of 570 nm. Meanwhile, one sample from each group was taken for its surface image using Scanning Electron Microscope (SEM). Result: The results showed that S. mutans biofilm mass in the group using static method was 0.34, while in the group using AMS method was 0.09. The results of the statistical analysis then showed that there was a significant difference (p=0.02) in the formation of bacterial biofilm mass between those groups. SEM image in the group using static method also showed that the attachment of S. mutans was more numerous and had a longer chain than in the group using AMS method. Conclusion: There is a difference in the profile of S. mutans bacterial adhesion between using AMS method and static method.

Inhibitory effects of siwak (Salvadora persica. L) extract on the growth of Enterococcus faecalis planktonics and biofilms by in vitro Ika Rhisty Cendana Sari; Rini Devijanti Ridwan; Diah Savitri Ernawati
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 3 (2016): September 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Enterococcus faecalis (E. faecalis) is one of the most persistent gram positive bacteria in root canal, resulting in secondary infection after endodontic treatment. E. faecalis pathogenicity is caused by overgrowth of E. faecalis planktonics and biofilms. E. faecalis planktonics produce lipoteichoid acid (LTA) as a virulence factor that can defend their permeability cell. On the other hand, E. faecalis biofilms produce protease, such as Esp (enterococcal surface protein), GelE (gelatinase), and SprE (serin protease), that have quorum-sensing mechanism as an adhesion factor to form extracellular polysaccharide substance (EPS) and increase the growth of the biofilms themselves. Siwak (Salvadora persica L.) has active components, namely benzylisothio-cyanate, trimethylamine, and salvadorine that can inhibit the growth of E. faecalis planktonics and biofilms. Purpose: This study aimed to measure inhibitory effects of siwak extract on the growth of E. faecalis planktonics and biofilms. Method: This research was an antimicrobial research on the culture of E.faecalis incubated in a TSB medium. Siwak extract was diluted into different concentrations, namely 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 75%, and 100%. The extract then was placed into the E. faecalis’s colony and planted into Trypticase Soy Agar medium. After incubated for 24 hours at 37°C, the colony would be measured and compared with the control (+) and control (-). As an antibiofilm research, this research used biofilm microtitter assay method to form E. faecalis biofilms incubated in a well-plate medium containing TSB and 0.1 % glucose. Siwak extract then was diluted into the same range concentration as in first method, and placed into the colony of E. faecalis to form biofilms. The biofilms were measured and compared to the control (+) given siwak extract and the control (-) given 0.1% chlorhexidine. After the incubation, they were washed three times, and staining process was conducted using Chrystal violet. The optical density then was measured by ELISA Reader 595 nm. Result: Siwak extract could inhibit the growth of E. faecalis planktonics at the concentration of 35% as a minimum inhibitory concentration as well as the growth of E. faecalis biofilms at the concentration of 45% as a minimum biofilm inhibitory concentration. Conclusion: Siwak extract has an inhibitory effect, particularly at a concentration of 35% on the growth of E. faecalis planktonics and at the concentration of 45% on the growth of E. faecalis biofilms.

Perceived parenting style and mother’s behavior in maintaining dental health of children with Down syndrome Siti Fitria Ulfah; Darmawan Setijanto; Taufan Bramantoro
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 4 (2016): December 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: The number of children with down syndrome in Surabaya has reached 924 children. Prevalence of gingivitis and dental caries (91% and 93.8%, respectively) occurs in children with down syndrome aged 6 to 20 years. Oral and dental health problems are found in children with down syndrome because they have physical and motoric limitation in maintaining oral and dental hygiene, thus require parental care from mother. Perceived parenting style includes responsiveness and demandingness. Perceived parenting is crucial for mother whose children have Down syndrome in order to guide their health behavior, particularly to maintain oral and dental health. Purpose: The study aimed to analyze correlation between perceived parenting style and mother’s behavior in maintaining dental health of children with Down syndrome. Method: This cross sectional analytical study involved 40 mothers of children aged 7-13 years with Down syndrome enrolled in Special Education Elementary Schools Surabaya and Association of Parents of Children with Down syndrome Surabaya. Data of perceived parenting style (responsiveness and demandingness) and mother’s behavior in maintaining dental health were obtained by questionnaire. Composition of each item in questionnaire of perceived parenting style and mother’s behavior in maintaining dental health of children with Down syndrome was passed through validity and reliability test. Data analysis was carried out using multiple linear regression correlation test. Result: This present study showed that perceived parenting style is significantly correlated with mother’s behavior in maintaining dental health of children with Down syndrome (R = 0.630, p = 0.000), with perceived parental responsiveness as a strong predictor. Mean score and standard deviation of perceived parental responsiveness and demandingness were 33.00±2.99 and 15.55±1.99, respectively. Conclusion: Perceived maternal parenting style in children with Down syndrome is closely related to mother’s behavior in maintaining dental health of children with Down syndrome.

The cleanliness differences of root canal walls after irrigated with East Java propolis extract and sodium hypoclorite solutions Tamara Yuanita
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Root canal instrumentation produces smear layer that covers dentine tubules of the root canal surface. Smear layer is organic and inorganic particles that have to be removed. East Java propolis extracts contais of saponin components used as a natural surfactant. 2.5% NaOCl and 5% NaOCl solutions have been widely used for irrigation in root canal treatment. Purpose: The purpose of this study was to analyze the cleanliness of the root canal walls, irrigated with aquadest, 8% East Java propolis extract, 2.5% NaOCl and 5% NaOCl. Method: Forty extracted teeth with straight single root canals were randomly divided into four groups(n=10). The specimens were prepared with ProTaper. During instrumentation, the root canals were irrigated with different solutions: Control Group irrigated with aquadest; Group 1 irrigated with 8% East Java propolis extract; Group 2 irrigated with 2.5% NaOCl and Group 3 irrigated with 5% NaOCl. The root canals were cut at apical third and SEM scores were tested by using Mann-Whitney test at the significance level of p=0.05 and Median Control test. Result: The results of Mann-Whitney Test, there were significant differences between control group with Group 1, 2 and 3 (p<0.05). Based on the Median Control test, the value of 8% East Java propolis extracts was 1,000, which was the best value compared to 2.5% NaOCl, 5% NaOCl and aquadest. Conclusion: It can be concluded that 8% East Java propolis extract is the most effective solution for cleaning root canal walls compared with 2.5% NaOCl and 5% NaOCl.

Scaffold combination of chitosan and collagen synthesized from chicken feet induces osteoblast and osteoprotegerin expression in bone healing process of mice Saka Winias; Diah Savitri Ernawati; Maretaningtias Dwi Ariani; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 2 (2017): June 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Over 500.000 of the 2,3 million surgical treatments requiring bone grafting procedures that are performed annually are likely to be necessitated by or will result in bone defects that will not regenerate. Treatment to regenerate new tissues is needed, especially for hard tissue repair, which not only relies on a natural osseointegration process, but also requires a physical support to guide the differentiation and proliferation of cells into the targeted functional tissue. Chitosan and collagen extracted from chicken feet combinations are expected to enhance the bioactive surface and provide mechanical strength as a bone graft scaffold. Purpose: The aim of this study was to investigate the role of chitosan and collagen scaffold synthesized from chicken feet applications to increase the expression of Osteoprotegerin (OPG) and osteoblast cells on the fourteenth day of bone healing. Methods: Eighteen three-month old, adult, male, Rattus novergicu strain rodents with a body weight ranging from 200-350 g were kept under controlled environmental conditions. The mice were randomly divided into three groups consisting of three subjects, each treated with collagen, chitosan, chitosancollagen combination (50:50) scaffolds. On the 14th post-treatment day, three members of each group were sacrificed. Examination of Osteoprotegerin (OPG) expression was conducted by means of immunohistochemistry staining with anti-OPG polyclonal antibodies. Meanwhile, osteoblast cell examination was performed by means of hematoxilin-eosin (HE) staining. Results: The mice treated with collagen and a chitosan-collagen combination scaffold presented an increase in the expression of Osteoprotegerin (OPG) and the number of osteoblast cells respectively. Conclusion: A combination of chitosan-collagen (50:50) scaffold extracted from chicken feet increased the expression of OPG and the number of osteoblasts in the bone healing process. The combination scaffolds demonstrated the highest OPG expression and number of osteoblasts compared to the other groups.

Gingival enlargement as oral manifestation in acute myeloid leukemia patient Sandra Olivia Kuswandani; Yuniarti Soeroso; Sri Lelyati C. Masulili
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 3 (2017): September 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Acute myeloid leukemia (AML) is a bone marrow cancer, a malignant disease that triggering the cells develops into different types of blood cells. It is widely recognized that the main manifestation of AML could be gingival hyperplasia and bleeding. Occasionally, an initial diagnosis of leukemia is made after a dental examination. In relation to systemic diseases, gingival enlargement could constitute the intensification of an existing inflammation initiated by dental plaque, or a manifestation of the systemic disease independent of the inflammatory condition of the gingiva. Gingival enlargement negatively affects the quality of life, especially nutritional intake. Purpose: This study aimed to report on gingival enlargement in AML patients, dental management of this condition and considerations when treating patients. Case: A 46 year-old woman diagnosed with AML who chiefly complained of gingival enlargement in all parts of the mouth which restricted her nutritional intake. Case management: The subject attended the clinic twice where nonsurgical treatment for the gingival enlargement, supragingival scaling and dental health education to maintain her oral hygiene was carried out. Unfortunately, she did not return for follow-up appointments due to having already passed away. Information about AML and its relation to gingival enlargement contained in the literature is also reviewed. Conclusion: In conclusion, gingival enlargement represents one oral manifestation of AML. This condition is related to and affects the nutritional intake of the patient.

Analysis of anti-Streptococcus sanguinis IgY ability to inhibit Streptococcus sanguinis adherence Suryani Hutomo; Heni Susilowati; Dewi Agustina; Widya Asmara
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 1 (2018): March 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Streptococcus sanguinis (S. sanguinis), an oral commensal bacterium, is often implicated in infective endocarditis. Its adherence to the tooth surface is the initial step in dental plaque formation. In addition to the important role of S. sanguinis in systemic disease and antimicrobial resistance, it is necessary to develop methods to control dental plaque formation. Immunoglobulin Y (IgY) has been used to prevent bacterial infection. Purpose: The purpose of this study is to analyze the ability of anti-S. sanguinis IgY antibodies to inhibit S. sanguinis adherence to hydroxyapatite (HA) discs as a model of the tooth surface. Methods: Antibodies were produced by immunizing hens with S. sanguinis suspension. Boosters were given three times following the first injection. An agar gel precipitation test (AGPT) was used to detect the presence of anti-S. sanguinis IgY. A bacterial adherence assay was performed twice to analyze the ability of IgY and the optimal concentration required to inhibit bacterial adherence. Results: The formation of a precipitation line using AGPT confirmed the presence of the antibody. In addition, it was shown that the anti-S. sanguinis IgY antibody could inhibit bacterial adherence to HA. Statistical analysis using One-way ANOVA revealed a significant difference in the optical density (OD) value between the groups (p<0.05). The results of electron microscopy scanning confirmed the quantitative analysis by means of a bacterial adherence test. Conclusion: Anti-S. sanguinis IgY has the ability to inhibit adherence of S. sanguinis to HA discs at an optimal concentration of 30%. The inhibitive effect was stronger in the presence of saliva.

Correlation of predictions to get a new dental caries with residence area and parental socio-economic conditions in adolescents in Sleman DIY Bambang Priyono; Hari Kusnanto; Al Supartinah Santoso; Dibyo Pramono
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 3 (2016): September 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Adolescence is a period when an individual experiences physical and psychological growth, thus requiring higher energy intake. As a result, they have a high appetite, but at the same time the supervision of parents on their oral hygiene behavior is decreases. They become free to choose their preferred food, sometimes containing high carbohydrates that may increase risks of dental caries and overweight. Sleman is one of districs in Yogyakarta, also considered as an agglomeration area of Yogyakarta town, which still has urban and rural areas. Purpose: This study aimed to examine the correlation between residence area and parental socioeconomic conditions with prediction to get a new dental caries. Method: This study was an analytic survey study conducted on 275 adolescents in Sleman. Samples were selected by using stratified cluster random sampling technique. Prediction to get a new dental caries was measured by using cariogram, involving 10 variables. Residence area was observed based on territorial characteristics, such as urban and rural areas matched to their ID card. Meanwhile, parental socio-economic condition was measured on daily expenses of their parents. A multiple regression analysis with dummy variables was used to analyses the correlation between the independent and dependent variables at a confidence level of 95%. Result: The results showed that the prevalence of caries in those adolescents in urban areas was 70.7%, while 81.95% in rural areas. The DMFT index in urban areas was 2.27, while 2.65 in rural areas. The mean percentage of prediction to get a new dental caries in urban areas was 47.83 ± 23.63, while 53.61 ± 24.68 in rural areas. The results of the statistical analysis then showed that there was no significant correlation of residence area and parental sosio-econimic conditions with prediction to get a new dental caries. Conclusion: In conclusion, residence areas, rural and urban areas, and parental socio-economic conditions, from low to high economic status were not correlated with prediction to get a new dental caries in adolescents.

The correlation between the use of personal protective equipment and level wild-type p53 of dental technicians in Surabaya Puspa Dila Rohmaniar; Titiek Berniyanti; Retno Pudji Rahayu
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 1 (2017): March 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Exposure of metals among dental technicians that come from the working environment can lead to the formation reactive oxygen species (ROS). ROS can cause mutations in the p53 gene (p53). The mutation is transversion mutation GuanineThymine. p53 mutations can lead to low expression of the wild-type p53 protein (p53). Wild-type p53 involved in many biological processes such as regulation of genes involved in cell cycle, cell growth after DNA damage, and apoptosis. However, exposure to metals among dental technicians can be prevented through the use of personal protective equipment (PPE) during work. Purpose: The purpose of this study was to analyze the correlation between the use of personal protective equipment to wild-type p53 protein levels among dental technicians in Surabaya. Method: This study was observational analytic with cross sectional approach. 40 samples were taken by random sampling. Data were retrieved through interviews and observations. Wild-type p53 was analyzed from saliva with indirect ELISA method. Analysis of data used Kolmogorov Smirnov normality test and a Pearson correlation test. Value significance was p<0.05 (95% confidence level). Result: There was a significant association between the use of personal protective equipment with wild-type p53 levels with p=0.002 Conclusion: The use PPE properly is positively correlated with the wild-type p53 protein levels of dental technicians in Surabaya.

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Contact Name
Saka Winias

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saka.winias@gmail.com

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Journal Mail Official
dental_journal@yahoo.com

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Location
Kota surabaya,

Jawa timur

INDONESIA