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Jurnal Veteriner

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Published by Universitas Udayana

ISSN : 14118327 EISSN : 24775665 DOI : https://doi.org/10.19087/jveteriner

Core Subject : Health,

Veterinary

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki

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Articles 18 Documents

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Search results for , issue "Vol 20 No 1 (2019)" : 18 Documents clear

Potensi Salep Epigallocatechin gallate terhadap Proses Kesembuhan Luka Bakar Derajat II pada Kulit Tikus Putih (THE POTENTIAL OF EPIGALLOCATECHIN GALLATE OINTMENT TO THE WOUND HEALING PROCESS OF SECOND DEGREE SKIN BURNS ON THE ALBINO RATS) Wiwik Misaco Yuniati; Bambang Sektari Lukiswanto
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Burns are one of the health problems in modern society that are associated with tissue damage that is difficult to repair and affect patients, both physically and psychologically. This study was conducted to evaluate the potential of epigallocatechin gallate (EGCG ) ointment to the healing process of second degree skin burn induced by attaching 85ºC plate with 1 cm of diameter for 5 second on the skin of albino rat (Rattus norvegicus). Twenty-five rats were divided into 5 treatment groups. The P0 group was a group of rat that suffered burns and were treated with ointment base (PEG). The P1 group was a group of rat that suffered burns and were given standard therapy with silver sulfadiazine. P2, P3 and P4 groups are groups of rat that have burns and are treated with EGCG ointments with concentrations of 1%, 2%, and 4% respectively. At the end of the study, skin tissue excision was carried out to make histopathological preparations using HE staining. Evaluation of histopathological preparations was carried out on reepithelialization collagen deposition, PMN infiltration, and angiogenesis. The results of the study in group P4 showed that the highest collagen formation and re-epithelialization process was accompanied by a marked decrease in the inflammatory process and angiogenesis. This condition is significantly different from groups P0, P1, P2, and P3. Healing second degree burns with 4% EGCG is better than other treatmentJurnal Veteriner Maret 2019 Vol. 1 No. 1 : 1-7 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.1 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/20162Proses kesembuhan luka bakar merupakan fenomena kompleks untuk mengembalikan kontinuitas jaringan dan fungsinya. Kesembuhan luka melibatkan beberapa fase yang berbeda dan saling tumpang tindih, yaitu fase inflamasi, granulasi, fibrogenesis, neo-vaskularisasi, kontraksi luka dan epithelialisasi (Robson, 1997). Penatalaksanaan luka bakar yang efektif memerlukan pemahaman proses kesembuhan luka normal dan mampu memilih intervensi yang tepat untuk mengoptimalkan proses kesembuhan luka (Snyder, 2005). Pada proses kesembuhan luka, inflamasi terjadi segera setelah jejas, diawali dengan vasokonstriksi yang berperan dalam proses hemostasis dan pelepasan mediator inflamasi. Fase proliferasi ditandai dengan terbentuknya jaringan granulasi oleh fibroblas dan proses angiogenesis. Reformulasi dan perbaikan kompartemen serabut kolagen yang disertai dengan penigkatan tensile strength menandai fase remodeling (Varoglu et al., 2010). Faktor yang memiliki peran penting pada tertundanya proses kesembuhan luka antara lain, trauma berulang, perfusi dan oksigenasi yang buruk serta inflamasi yang berlebihan (Harding et al., 2003)). Penggunaan bahan alamiah untuk pengobatan luka merupakan bagian penting dari penatalaksanaan kesehatan dan metode baik untuk menyediakan pilihan layanan kesehatan yang murah dan efektif (Gurung et al., 2009; Suntar et al., 2010). Beberapa penelitian menggunakan polifenol yang berasal dari teh hijau sebagai penyembuh alami sebagai agen anti penuaan, antiinflamasi, antikanker, antioksidan dan antidiabetes (Obaid et al., 2011). Beberapa penelitian dengan menggunakan Epigallocatechin gallate (EGCG), sebagai salah satu polifenol yang terkandung dalamteh hijau, membuktikan sejumlah efek biologis EGCG sebagai antioksidan, antimikrob, antiinflamasi, antialergi dan antineoplastik yang aktif (Hosnuter et al., 2015). Teh hijau memiliki manfaat yang beragam, antara lain mencegah kanker, meningkatkan kesehatan jantung dan pembuluh darah, melindungi kulit dari kerusakan yang disebabkan karena radiasi dan penyebab yang lain. Manfaat ini disebabkan karena the hijau yang mengandung EGCG memiliki aktivitas antioksidan, antiinflamasi dan antibakteri yang cukup kuat. Berdasarkan hal tersebut, tidak menutup kemungkinan bahwa EGCG dapat membantu percepatan proses kesembuhan luka bakar pada kulit (Nagle et al., 2006). Berdasarkan uraian tersebut, penelitian ini dilaksanakan untuk melihat pengaruh pemberian salep EGCG terhadap proses kesembuhan luka bakar pada tikus putih (Rattus norvegicus) jantan.METODE PENELITIAN Kelayakan Etik Penelitian ini dilaksanakan setelah mendapatkan persetujuan dari komisi etik Fakultas Kedokteran Hewan, Universitas Airlangga. Tempat Penelitian Penelitian ini dilakukan di Laboratorium Hewan Percobaan di Fakultas Kedokteran Universitas Airlangga, Surabaya. Persiapan dan pembuatan salep dilakukan di Fakultas Farmasi, Universitas Airlangga. Pembuatan dan pemeriksaan preparat histopatologis dilakukan di Laboratorium Patologi Anatomi, Fakultas Kedokteran Hewan, Universitas Airlangga. Hewan Eksperimental Sebanyak 25 ekor tikus putih (Rattus norvegicus) jantan, umur tiga bulan dengangroups. This is presumably because the compounds contained therein have antioxidant activity, antiinflammatory and antibacterial. These three activities will synergize in the process of healing wounds. Provision of 4% EGCG ointment for 14 days in second degree burns can accelerate the wound healing process which is characterized by improved re-epithelialization, collagen deposition, PMN infiltration in the wound area, and angiogenesis.

Kerusakan Deoxyribonucleic Acid (DNA) Spermatozoa Memengaruhi Tingkat Kebuntingan Sapi Brahman (DAMAGE TO DEOXYRIBONUCLEIC ACID (DNA) SPERMATOZOA AFFECTING THE LEVEL OF PREGNANCY IN BRAHMAN CATTLE) Langgeng Priyanto; Agung Budiyanto; Asmarani Kusumawati; Kurniasih Kurniasih
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The relationship among of sperm DNA damage in cows with pregnancy rates has not been widely studied. The purpose of this study to determine the relationship of sperm DNA damage with pregnancy rate on Brahman cows. The sperm DNA damage rate was measured by Sperm-BosHalomax® from 2 samples of male Brahman bull straw (40002 and 40885) and pregnancy rate was measured from the success rate of artificial insemination. In 14 female Brahman cows divided into two groups. One group of 7 in the artificial insemination with 40002 males with 37.11% sperm DNA damage and one in artificial insemination with 40885 with 10.65% sperm DNA damage. The data obtained were analyzed descriptively by comparing sperm DNA damage with pregnancy rate. The results showed that at 37.11% sperm DNA damage level was found pregnancy rate 57.11% with ultrasound on 30 day and pregnancy rate 42.80% with ultrasound to 45 day. Result of research on sperm DNA damage level of 10.66% found pregnancy rate 57.11% with ultrasound to 30 day and level pregnancy 57.11% with ultrasound 45 days. The results of this study have concluded that there is a difference in the rate of sperm DNA damage with pregnancy rate in Brahman cows. The sperm DNA damage has an effect on pregnancy rate on Brahman cows.

Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Daging Ayam pada Pasar Tradisional di Kota Bogor (ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM CHICKEN MEAT OF TRADITIONAL MARKETS IN THE CITY OF BOGOR) Connie Januari; Mirnawati Bachrum Sudarwanto; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic use in farm is spread widely to treat of poultry disease including therapy, supportive or preventive use and as afeed additive to improve chicken performance. The negative effects of antibiotic use can increase the level of bacterial resistance to antibiotics. This study aimed to investigate on antibiotic resistance in Escherichia coli isolated from chicken meat that were sold in Traditional Market of Bogor City. A total of 175 samples of chicken meat were taken by purposive sampling method, out of 175 found 50 positive samples of E. coli. The samples were subjected to E. coli examination and the isolated E. coli were tested for the antibiotic resistance using eight antibiotics, i.e., amoxicillin, cefotaxime, colistin, nalidixid acid, streptomycin, erythromycin, oxytetracillin, and tetracycline. The study was conducted by using the disk diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute guidelines. The study showed E. coli isolated from chicken meat were resistance towards amoxicilin (90%), colistin (94%), nalidixid acid (86%), streptomycin (98%), erythromycin (98%), oxytetracillin (84%), tetracycline (86%), and cefotaxime antibiotics (12%). The proportion of multidrugresistant was 99%. The higher of multidrug-resistant indicated the E. coli would be a threat to public and environmental health.

Estimation of Aflatoxin M1 Exposure through Consumption of Various Dairy Milk Products in Yogyakarta, Indonesia (ESTIMASI PAPARAN AFLATOKSIN M1 MELALUI KONSUMSI BERBAGAI PRODUK SUSU DI YOGYAKARTA, INDONESIA) Ika Sumantri; Fitri Purwanti; Nuryono Nuryono; Ali Agus
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was conducted to investigate the occurrence of aflatoxin M1 (AFM1) in various milk products marketed in Yogyakarta Province (Indonesia) and to estimate the exposure of aflatoxin through contaminated milk consumption. Fresh milk (n=20), pasteurized milk (n=16), and recombined milk products (n=6) were sampled for AFM1 concentration testing by a competitive ELISA test using ELISA kit for AFM1 assay. A survey was conducted to interview consumers (n=88) on milk consumption habit (milk type and amount of consumption). ELISA assays showed 92.5% of samples werecontaminated with AFM1 in a range of 24-570 ng/L (average: 216 ng/L). The highest average AFM1 concentration was detected in pasteurized milk sample (244 ng/L), followed by fresh milk (219 ng/L), and the lowest was in recombined milk sample (131 ng/L). However, 100% of recombined milk samples had AFM1 concentration >50-500 ng/L. Thus, recombined milk product was most likely the main source of AFM1 intake due to its high daily consumption in all age groups. Based on AFM1 levels found in milk and consumption of corresponding milk sample, it was estimated that the overall AFM1 exposure ranges from 1.23 ng/kg body weight/day (in 6-15 year-old children) up to 5.26 ng/kg body weight/day (in 3-5 year-old children). In conclusion, this study revealed high occurrences of AFM1 dairy milk marketed in Yogyakarta. Although levels of AFM1 contamination were in Indonesian regulatory limit, high exposure of aflatoxin found in all age groups of consumer. Thus, this preliminary study provides evidence that AFM1 contaminated milk is a serious public health hazard in Indonesia.

Ekstrak Etanol Kulit Buah Jengkol Menurunkan Kadar Glukosa dan Meningkatkan Hormon Insulin Tikus Diabetes Yang Diinduksi Streptozotocin Desak Made Malini; Madihah Madihah; Dita Aprila Khoirunnisa; Indriani Sasmita; Nining Ratningsih; Kartiawati Alipin; Wawan Hermawan
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Diabetes Mellitus (DM) is a disease with metabolism disorder signed by increasing of glucose blood (hyperglicemia), caused by disorder of insulin secretion and or increasing of insulin resistance. Therapy of jengkol’s fruit peel is an alternative treatment for DM. This study aims to determine the effect of ethanol extract of jengkol’s fruit peel to decrease glucose and increase insulin level of Wistar female rats which induced by streptozotocin (STZ). This research used the experimental method of Completely Randomized Design (RAL). Rat were first induced by single intravenous injection of STZ except Negative Control. Furthermore, rats were divided into six groups, namely Negative Control (NC), Positive Control (PC), Reference Group (RG) glibenclamide 10 mg/kg BW, and ethanol extract of jengkol’s fruit peel (EEOJFP) treatment at dose of 385 (P1), 770 (P2), and 1540 (P3) mg/kg BW with four replications each. Treatment was administered orally for 14 days. Data were analyzed by analysis of variance (ANAVA) with 95% confidence level and Duncan Multiple Range Test. The result showed that treatment of EEOJFP with dose of 385, 770 and 1540 mg/ kgBW decreased blood glucose 28.6%;65.4% and 59.1% successively, increased insulin as 54%; 230% and 206%. There was a protective effect of EEOJEF to level of blood glucose and insulin secretion of rat-STZ induced. The conclusion from this research is Ethanol extract of Jengkol’s fruit peel at dose 770 mg/kg BW is an effective dose in decreasing the level of glucose and increasing the level of insulin hormone in STZinduced diabetic rats.

The Insidensi dan Derajat Infeksi Anisakiasis pada Ikan Hasil Tangkapan di Pelabuhan Perikanan Pantai Muncar, Banyuwangi, Jawa Timur (INCIDENCE AND DEGREE OF ANISAKIASIS INFECTION IN FISH CATCHES AT THE FISHERY PORT OF MUNCAR BEACH, BANYUWANGI, EAST JAVA) Mohammad Faizal Ulkhaq; Darmawan Setia Budi; Muhammad Hanif Azhar; Hapsari Kenconojati
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The purpose of this study was to determine the incidence and degree of infection anisakiasis in fish catches at Fishery Seaport Muncar, Banyuwangi as an effort to prevent food borne diseases in humans. Fish samples from Muncar Beach Fishery Seaport, Banyuwangi consisted of tuna fish (Euthynnus sp.), bloated fish (Rastrelliger sp.), giant trevally fish (Caranx sp.) and snapper (Lutjanus sp.) with total amount 192 tail. Sample fish was dissected and examined the parasite by native method. Parasitic worms was fixed with 5% glycerine alcohol, stained with SemichenAcetic Carmine method, identified by key identification and calculated incidence and degree of infection. The highest incidence and degree of infection showed in Lutjanus sp. and Rastrelliger sp. 66.67% and 5.39, respectively, while the lowest showed in Caranx sp. from 6.67% and 0.09 respectively. Continue research to determine incidence and degree of infection anisakiasis in each season was required to mapping and to know the spread of worms Anisakis sp.

Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali (THE EFFECTIVENESS OF GUAVA FILTRATE SUPPLEMENTATION IN COCONUT WATER-EGG YOLK DILUTION ON QUALITY OF LIQUID SEMEN OF BALI CATTLE) ALoysius Marawali; Muhammad S. Abdullah; Jalaludin Jalaludin
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The aim of this research was to know the effectiveness of guava filtrate supplementation in coconut water- egg yolk dilution on quality of liquid semen stored at 5oC of Bali cattle. Semen collected from a five year old Bali cattle using artificial vagina. Semen of good quality were kept in six tubes based on treatment then stored at 5oC. Treatments of the research were P0 : coconut water 80% + egg yolk 20% without guava filtrate; P1 : coconut water 80% + egg yolk 20% + 0.8% guava filtrate; P2 : coconut water 80% + egg yolk 20% + 0.9% guava filtrate; P3 : coconut water 80% + egg yolk 20% + 1.0 % guava filtrate; P4 : coconut water 80% + egg yolk 20% + 1.1 % guava filtrate and P5 : coconut water 80% + egg yolk 20% + 1.2 % guava filtrate. Each treatment was replicated 8 times making 48 experimental units. Results of the study showed that percentage mean of motility, viability, MPU, and TAU of spermatozoa after three days storage for P0 were : 42.20%, 41.85%, 39.08% and 40.90%; P1 : 50.40%, 53.89%, 52.99% and 54.67%; P2 : 54.67%, 56.97%, 54.51% and 54.36%; P3 : 17.00%, 29.96%, 29.64% and 29.64%; P4 : 23.38%, 24.64%, 21.06% and 24.45%Jurnal Veteriner Maret 2019 Vol. 20 No. 1 : 20 -29 pISSN: 1411-8327; eISSN: 2477-5665 DOI: 10.19087/jveteriner.2019.20.1.20 Terakreditasi Nasional, Dirjen Penguatan Riset dan Pengembangan, online pada http://ojs.unud.ac.id/index.php/jvet Kemenristek Dikti RI S.K. No. 36a/E/KPT/201621PENDAHULUAN Salah satu solusi yang dapat digunakan untuk pengembangan program Inseminasi buatan (IB) secara cepat dan mudah pada sapi bali adalah penggunaan semen cair. Penggunaan semen cair dapat meningkatkan kinerja IB pada sapi bali di Nusa Tenggara Timur (NTT). Keunggulan lain semen cair dapat diproduksi menggunakan bahan pengencer herbal berbasis bahan lokal dan peralatan yang sederhana serta mudah diperoleh dan tidak tergantung dengan persediaan nitrogen cair. Hasil akhir dari metabolisme spermatozoa adalah terbentuknya radikal bebas berupa derivat oksigen di antaranya adalah single1 oksigen (1O2), tripel1 oksigen (3O2), superokside anion (O2-), hidroksil radikal (OH) dan nitrit oxide (NO-) yang semuanya disebut radical oksigen species (ROS). Single1 oksigen dapat merusak ikatan rangkap pada asam lemak sehingga dapat menyebabkan kerusakan Deoxyribo Nuclead Acid (DNA) dan protein. Single1 oksigen bila bereaksi dengan asam amino histidin akan membentuk enzim yang dapat menyebabkan denaturasi protein. Kerusakan spermatozoa pada penyimpanan suhu 5%C akibat radikal bebas dan cold shock inilah merupakan penyebab utama disfungsi semen (Sharma et al., 2000). Oksidasi fosforilasi yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang terganggu menyebabkan peningkatan radikal bebas dalam semen. Kadar radikal bebas yang tinggi dalam sel dapat mengoksidasi lipid, protein dan DNA. Lipid membran plasma semen memiliki fosfolipid dengan kadar yang tinggi menyebabkan semen rentan terhadap radikal bebas (Sanoeka dan Kurpisz, 2004). Antioksidan bertindak mengikat asam lemak tak jenuh dan mencegah terjadinyareaksi berantai. Pada proses penyimpanan semen akan terjadi kerusakan membran plasma spermatozoa akibat terbentuknya perioksidasi lipid. Antioksidan-pemutus rantai seperti yang terkandung dalam jambu biji dapat menghambat perioksidasi lipid dalam membran melalui radical peroxyl (RO) dan alkoxyl (ROO) pengurai. Pengunaan jambu biji yang difilter dalam pengencer air kelapa kuning telur dapat menjaga kualitas spermatozoa (motilitas, keutuhan akrosom, viabilitas dan morfologi spermatozoa) semen cair sapi bali selama penyimpanan pada suhu 5%C. Dosis jambu biji yang difilter yang terbaik dalam pengencer air kelapa kuning telur, akan terbaik pula dalam mempertahankan kualitas spermatozoa sampai tujuan IB. Adapun tujuan penelitian ini adalah menguji berbagai level pemberian filtrat jambu biji (FJB) dalam pengencer air kelapa kuning telur terhadap motilitas, viabilitas, membran plasma utuh (MPU) dan tudung akrosom utuh (TAU) spermatozoa sapi bali yang disimpan pada suhu 5%C.METODE PENELITIAN Penelitian ini telah dilakukan di Laboratorium Reproduksi milik Yayasan Wiliams dan Laura yang berlokasi di Tilong, Desa Oelnasi, Kec. Kupang Tengah, Kab. Kupang, Nusa Tenggara Timur, dan berlangsung selama delapan bulan. Materi yang digunakan dalam penelitian ini adalah semen sapi bali yang ditampung dari satu ekor sapi bali jantan berumur lima tahun milik Yayasan Williams dan Laura yang telah dilatih, memiliki performans yang baik, dan organ reproduksi normal. Pakan yang diberikan adalah hijauan berupa rumput dan legum dan pemberian konsentrat secukupnya (dedak padi dan jagung giling).and P5 : 9%, 21.25%, 17.56% and 19.30%. Result of statistical analysis showed that there were a significant effect (P<0.05) between treatment on motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage. It can be concluded that the supplementation of guava filtrate 0.9% in dilution of coconut water 80% - egg yolk 20% had been able to maintain motility, viability, MPU and TAU of spermatozoa of Bali cattle till the third day of storage at 5oC.

Identifikasi Bakteri dan Efektivitas Antibiotik dalam Pengencer untuk Mengontrol Pertumbuhan Bakteri pada Semen Sapi Friesian Holstein Muttaqinullah Rabusin; Andriani Andriani; Raden Iis Arifiantini; Ni Wayan Kurniani Karja
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was designed to investigate the presence of bacterial species in Friesian Holstein (FH) bovine semen at the time of collection, processing and to assess the efficacy of two types of antibiotics combinations; penicillin and streptomycin (PS) and gentamycin, tylosin, lincomycin and spectinomycin (GTLS) in semen extender on bacterial control and quality of semen. For this purpose, three experiments were conducted. In experiment 1, identification of bacterial content in fresh semen which collected from 5 bovine ejaculates. In experiment 2, identification of bacterial content in skimmilk-eggyolk extender which were prepared in artificial insemination center, Lembang, Bandung. In experiment 3, identification of bacterial content in frozen thawed semen. In the result, some of bacterial species were isolated from the bovine semen. The GTLS combination of antibiotics may be incorporated into a freezing extender or protocol without compromising the post-thawed semen quality of FH bull spermatozoa. Three types of bacteria were found in fresh semen; Klebsiella sp., Micrococcus sp., and Pediococcus sp.. Three types of bacteria were found in semen extender; Enterobacter cloacae, Pseudomonas diminuta and Serratia plymutica. Two types of bacteria were found in frozen semen; Enterobacter cloacae and Serratia plymutica. In conclusion, antibiotics PS and GTLS were effective for controlling the growth of bacteria in frozen semen.

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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