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INDONESIA
BIOMA : Jurnal Biologi Makassar
Published by Universitas Hasanuddin
ISSN : 25287168     EISSN : 25486659     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Bioma mempublikasikan hasil penelitian dan kajian biologi: ekologi, botani, genetika, mikrobiologi, zoologi maupun biologi terapan dibidang agrokomplek dan medikal komplek. Bioma diterbitkan Departemen Biologi, FMIPA UNHAS, dan mempublikasikan artikel dua kali setahun , setiap bulan juni dan desember
Arjuna Subject : -
Articles 218 Documents
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EKSPLORASI IN SILICO GEN rbcL PADA Dioscorea alata: KERAGAMAN GENETIK UNTUK KETAHANAN PANGAN MASA DEPAN Wa Ode, Kamillah
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Global food security is increasingly threatened by climate change, land degradation, and the heavy dependence on rice as the principal staple food. Diversification through the utilization of local commodities offers a strategic solution, with Dioscorea alata L. (purple yam), a tuberous crop rich in carbohydrates and anthocyanins and exhibiting high adaptability to diverse agroecological conditions, presenting significant potential. This study aimed to investigate the genetic diversity of D. alata through an in silico approach, employing the rbcL gene as a molecular marker, with sequence data retrieved from the public NCBI database. Analytical procedures included BLAST alignment, multiple sequence alignment, phylogenetic tree construction, 3D protein structure prediction, and functional annotation. The findings revealed that the rbcL gene in D. alata is highly conserved, with 100% sequence identity among accessions, suggesting pronounced genetic homogeneity likely resulting from long-term domestication. In contrast, sequence variation among other Dioscorea species indicated evolutionary divergence. The predicted protein structure displayed a predominance of alpha helices, supporting structural stability critical for photosynthetic function. Additionally, the rbcL gene was located within the active domain of the RuBisCO enzyme, essential for carbon fixation in the Calvin cycle. Thus, while rbcL is effective for evolutionary studies and interspecies identification, complementary markers such as matK or ITS are recommended for resolving intraspecific genetic diversity. The present findings are anticipated to contribute to the conservation and breeding strategies of D. alata in support of future food security initiatives. Keywords: Dioscorea alata, rbcL, DNA barcoding, food security
DESAIN PRIMER GEN EDNRB EKSON 1 UNTUK ANALISIS MUTASI GEN PADA HIRSCHSPRUNG’S DISEASE Wahyuni, Nur Anisa; Yuni Ahda
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

The endothelin receptor type B (EDNRB) gene is located on chromosome 13, consisting of 7 exons and 6 introns. The EDNRB protein produced from this gene belongs to the family of G-protein coupled receptors. Mutations in the EDNRB gene play a role in some cases of Hirschsprung's cause, although the frequency is low. This study aims to design primers and test specificity using BLAST primers to determine the specific primer attached to the EDNRB gene. Primers were designed using Geneious Prime, which were then analyzed in silico based on good primer criteria. The results of this research showed that primers made using Geneious Prime produced the best primers, namely forward primer 5'-GAGTTCGGAGCTTTGCCT-3' and reverse 5'-AGCCCACCA TGATTTCA GC-3' and the results of the specificity test showed that the primer specifically attached to chromosome 13 which is the location of the EDNRB gene (13q22) with a product length of 879 bp. Kata kunci : EDNRB Gene, Primer Design, Hirschsprung’s Disease
PENGARUH KONSENTRASI ASAM NITRAT SEBAGAI REAGEN DEKALSIFIKASI TERHADAP KUALITAS PEWARNAAN SAFRANIN O PADA TULANG Yusdarfadri, Rita Permatasari; Verdian Putra, Fuji
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

In histotechnology, bones play a certain role in the diagnosis of disease. The dilemma experienced by an anatomical pathology laboratory technician in making bone preparations or preparations containing high calcium salts. To overcome the hardness of the tissue due to the presence of calcium salts, a special technique called decalcification is needed. Strong acids such as hydrochloric or nitric acid are the fastest decalcification solutions in the decalcification process. Safranin is a cationic dye used in histology and cytology to differentiate and identify various tissues and cells. This study aims to determine the effect of nitric acid concentration as a decalcification reagent on the quality of safranin O staining in bones. This study uses an experimental method. The samples used in this study were beef ribs, then the specimens were decalcified with 5%, 10% and 15% nitric acid. The results showed that the nitric acid decalcification agent at concentrations of 5% and 10% obtained a consistency result of 1, meaning that the consistency of the beef rib bone sample was not soft, while 15% Nitric Acid obtained a consistency result of 3, meaning that the consistency of the beef rib bone sample was soft. Then, in the histological assessment of bone tissue preparations that have been stained with Safranin O, it was obtained at a concentration of 5% staining intensity with a final score grade of 0, meaning poor quality. At a concentration of 10% staining intensity with a final score of 1, meaning poor quality, and histological assessment of a concentration of 15% staining intensity with a final score of 2, meaning good quality. From this study, it can be concluded that the results of safranin O staining on beef ribs show differences in staining quality at different concentrations of decalsifying agents.
Inovasi Nanokoloid Perak Hasil Biosintesis Menggunakan Ekstrak Tanaman Keladi Sarawak Dalam Bentuk Gel : Uji Stabilitas Fisik Dan Aktivitas Antibakteri Terhadap Staphylococcus aureus Athiah Masykuroh; Dzawil Kamala, Sirna; Sintia Puspita, Rani
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Stability test of Physical and Antibacterial Activity against Staphylococcus aureus of the silver nanocolloid gel biosynthesized by Alocasia macrorrhizos plant extract has been conducted. The test was using cycling test method to estimate stability of the gel. The AgNO3 concentration used in the silver nanocolloid biosynthesis process is 0.05 M ; 0.10 M and 0.15 M. The nanocolloid was diluted 50% of the initial concentration and innovated into a gel form (formula I, II and III) then tested on physical evaluation and antibacterial activity against Staphylococcus aureus bacteria. The physical evaluation test include organoleptic, pH, homogeneity, viscosity, spreadability and hedonic test. The measurement of of the clear zone formed around the disc paper was carried out to determine it’s antibacterial activity. The results shows all variation’s value decreased at physical evaluation test but still meet the requirements except for viscosity test and shows decereased at antibacterial activity test against Staphylococcus aureus. Kata kunci : silver nanocolloids, gel, Alocasia macrorrhizos (L.), cycling test, staphylococcus aureus
EKSTRAKSI DAN DETEKSI KANDUNGAN FITOKIMIA DAUN SALAM (Syzygium polyanthum) Lestari, Devi Eka; Indriyani; Susanti, Nida Lidya
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Bay leaf (Syzygium polyanthum (Wight) Walp.) is one of the commonly used spices in cooking, known for enhancing the taste and aroma of food as well as offering health benefits. This study aims to identify the phytochemical content of bay leaves. The extraction method employed was maceration using 96% ethanol. The bay leaves were dried through aeration until the moisture content reached below 10%. The dried samples were then homogenized using a homogenizer to obtain a fine powder. The resulting powder was macerated with 96% ethanol. The obtained extract was then qualitatively tested for its phytochemical contents, including alkaloids, saponins, steroids, flavonoids, terpenoids, tannins, and phenolics. The results showed positive reactions for alkaloids with three reagents (Mayer, Boucardon, and Dragendorff), indicated by the formation of white, orange, and brown precipitates. Flavonoids were detected by a yellowish-green color change, while saponins produced stable foam. Tannins, steroids, and phenolics showed a dark green coloration, indicating the presence of these compounds. In contrast, the test for terpenoids yielded negative results. These findings indicate that bay leaf extract contains various secondary metabolites with potential antioxidant, antimicrobial, and other pharmacological activities. Keywords: Extraction, Phytochemical Detection, Bay Leaf
PRODUKTIVITAS SERASAH Rhizophora apiculata Blume danAvicennia marina (Forsk.) Vierh. DI HUTAN MANGROVE KELURAHAN UNTIA, KOTA MAKASSAR Muhammad Ruslan Umar; Ainun Saputri
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

The role of mangrove forests as a home for aquatic biota is insepar-able from their function as producers of litter such as leaf litter, flowers, twigs and fruits, which can be converted into nutrients. These nutrients are reabsorbed by mangrove plants for growth and partially utilized by other organisms in the water.  This study aims to analyze the productivity of mangrove litter Rhizophora   apiculata Blume and Avicennia marina(Forsk.) Vierhin supporting water fertility in Untia Village, Makassar City. The research method was conducted with non-destructive vegetation analysis using litter traps, conducted in R. apiculata area (Station I) and A. marina area (Station II), and each consists of 3 sampling points. Litter was collected every other day for a period of two months. The average daily litter productivity of A. marina was highest at sampling site III with 7.06 grams/m2/day, while for R. apiculata it was 2.93 grams/m2/day at sampling site II. In general, the average daily productivity of Avicennia marina litter was 5.45 grams/m2/day, higher than the average daily productivity of Rhizophora apiculata litter, which was 2.76 grams/m2/day. A. marina litter produc-tivity reached an average of 19.89 tons/ha/year, while R. apiculata litter productivity reached an average of 10.09 tons/ha/year. The productivity of the mangrove forest litter in the urban village of Untia is classified as a good criterion for supporting water fertility. Keywords: productivity, litter, Rhizophora apiculata,Avicennia marina  
INVENTARISASI JENIS TUMBUHAN YANG BERPOTENSI MENINGKATKAN KETAHANAN PANGAN DI KEPULAUAN SPERMODE SULAWESI SELATAN Priosambodo, Dody
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 10 No. 2 (2025): Bioma : Juli - Desember 2025
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

The Spermonde Islands are a group of small islands off the west coast of South Sulawesi that have characteristics of rich coastal and marine ecosystems, but are vulnerable to climate change pressures and limited food resources. This study aims to identify local plant species that have the potential as alternative food sources to support community food security on these small islands. The method used was an exploratory survey with a participatory approach, involving local communities on five selected islands. The inventory results showed thatthere were 31 types of potential food plants, including tubers, local fruits, vegetables, and wild plants that could be used as sources of carbohydrates, vegetable protein, and micronutrients. Several species such as Artocarpus altilis (breadfruit), Moringa oleifera (moringa), and Pandanus tectorius (sea pandan) have high nutritional value and good ecological adaptation. This study recommends the utilization and preservation of local plants as a strategy to strengthen small island-based food security. Kata kunci : food security, local plants, small islands, spermonde islands, biodiversity
PENETAPAN KADAR FLAVONOID TOTAL EKSTRAK ETANOL BUNGA PULUTAN ( Urena lobata L) DENGAN METODE SPEKTROFOTOMETRI UV-Vis Athiah Masykuroh; Ummu Khalifatul Ummah
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 2 (2024): Bioma : Juli - Desember 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Bunga pulutan (Urena lobata L.) memiliki kandungan metabolit sekunder yang efektif sebagai antioksidan sehingga mampu menangkal radikal bebas. Penelitian ini bertujuan untuk mengetahui kadar flavonoid total ekstrak etanol bunga pulutan dengan metode spektrofotometri UV-Vis. Penetapan kadar flavonoid total ekstrak etanol bunga pulutan dilakukan dengan spektrofotometri UV-Vis dengan panjang gelombang maksimum 421,5 nm. Hasil penelitian menunjukkan bahwa ekstrak etanol bunga pulutan mengandung senyawa flavonoid dengan kadar flavonoid total ekstrak etanol bunga pulutan yaitu sebesar 20,84%.
POTENSI GETAH LIDAH BUAYA Aloe vera L. TERHADAP PENYEMBUHAN LUKA BAKAR PADA TIKUS Rattus novergicus Berkenhout Evi Erviani, Andi; Resky Aulia; Elis Tambaru
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 9 No. 2 (2024): Bioma : Juli - Desember 2024
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

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Abstract

Lidah buaya memiliki efek anti-inflamasi yang berpotensi signifikan, oleh karena itu dapat digunakan dalam mengobati: gingivitis, dan luka bakar tingkat pertama hingga kedua. Tanaman lidah buaya mengandung senyawa yang dapat membantu proses penyembuhan luka bakar seperti tanin dan flavonoid. Penelitian ini bertujuan untuk menganalisis efektivitas getah tanaman Lidah buaya Aloe vera L. sebagai penyembuh luka bakar dengan menggunakan 12 ekor tikus yang diberi luka bakar pada bagian punggung dengan menggunakan plat besi berdiameter 2 cm. Perawatan luka bakar dilakukan setiap hari selama 12 hari dengan 4 kelompok perlakuan yaitu kontrol negatif (K1), kontrol positif (K2), getah lidah buaya 75% (K3), dan getah lidah buaya 100% (K4). Parameter yang diamati yaitu persentase kesembuhan luka bakar pada hari ke- 1, 4, 8, dan 12. Persentase penyembuhan luka terbaik terlihat pada hari ke-12 yaitu getah lidah buaya 100% (K4) dengan hasil 95.99%. Sehingga dapat disimpulkan bahwa pengaplikasian getah lidah buaya 100% lebih baik dalam penyembuhan luka bakar dibandingkan kelompok perlakuan lainnya. Kata Kunci : Getah lidah buaya, Luka bakar, Penyembuhan luka bakar
STUDI PERILAKU MONYET EKOR PANJANG (Macaca fascicularis) DI KAWASAN KAMPUS UNJA MENDALO Aditiya, Randi; Meitriani, Yohana; Saragih, Rasmita Berliana; Angelita, Antra; Hanum, Nabila Asri; Ramadhona, Octory Fillia; Hombing, Apriana Br; Adriadi, Ade; Suprayogi, Dawam
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 11 No. 1 (2026): Bioma : Januari - Juni 2026
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v11i1.44515

Abstract

This study aims to explain the daily activity patterns of long-tailed monkeys (Macaca fascicularis) in the area of ​​the University of Jambi (UNJA) Mendalo Campus. This study was conducted using a descriptive quantitative approach through the focal animal sampling method in four main locations (UNJA Jami' Mosque, UNJA Lake, UNJA LPTIK, and UNJA FKIP). The research location was determined by purposive sampling, namely in areas inhabited by long-tailed monkey populations, at three different times (morning, afternoon, evening). In each location, one individual monkey was observed for 1 hour per period with behavioral recording every 5 minutes. The results of the study showed that the most common behaviors were eating, moving, and grooming, with activity patterns varying in each location and time. Locations that have open food sources, such as UNJA Jami' Mosque, tend to be the focus for eating and grooming activities, while quieter locations such as UNJA Lake and LPTIK support playing and grooming activities. Environmental factors, such as food availability and the level of human disturbance, have a significant impact on monkey activity patterns. The results of this study are expected to be a basis for managing a campus environment that is balanced between human activities and the existence of wildlife. Keywords : Macaca fascicularis, Daily behavior, Behavioral ecology, Jambi University campus, Focal animal sampling, Semi-urban environment                                           

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