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JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

JHPT TROPIKA
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Published by Universitas Lampung

ISSN : 14117525 EISSN : 24610399 DOI : -

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Jurnal Hama dan Penyakit Tropika (JHPT Tropika) publishes articles in plant pests, plant pathogens, plant damage caused by those pests and pathogens and or their management in tropical areas. In addition to basic and applied research papers, JHPT Tropika publishes short communication that have not been published. Before being accepted for publication, all manuscripts must be peer reviewed. The journal is published sixmonthly in March and September.

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Articles 521 Documents

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ASAI KEMAMPUAN BAKTERI ENDOFIT DARI KACANG TANAH DALAM MENGHAMBAT PERTUMBUHAN Sclerotium sp. PADA KECAMBAH KACANG TANAH Lisa Novita Arios; Dwi Suryanto .; Kiki Nurtjahja .; Erman Munir .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings. A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method. In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.

KERAGAMAN GENETIK, KEBUGARAN DAN INKOMPATIBILITAS REPRODUKSI Hemiptarsenus varicornis Girault (Hymenoptera: Eulophidae), PARASITOID LARVA Liriomyza huidobrensis (Diptera: Agromyzidae) . Reflinaldon; Damayanti Buchori; Dwinardi Aprianto
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 11 No. 1 (2011): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Several experiments have been conducted to study genetic variation, fitness and reproductive incompatibility of H. varicornis from different geographic populations. Genetic variation from Pandai Sikek (PS), Alahan Panjang (AP) and Kayu Aro (KA) was analyzed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique and the similarity of genetics measured using NTSys program. The fitness of female wasps such as longevity, fecundity and preoviposition was observed and then compared among those populations. Incompatibility in reproduction was determined by accounting of reproductive compatibility (RC) index in crossing of intra and interpopulation both of PS and AP. The results showed high genetic variation of H. varicornis among population from Alahan Panjang, Pandai Sikek and Kayu Aro with similarity coefficient of 30 to 70%. The best fitness showed the female wasps from Kayu Aro that was significantly different (P= 0.00) in longevity (24.60 ± 6.4 days), fecundity (63.6 ± 28.6 eggs) and parasitization (53.60%) but not significantly different (P=0.07) in number of the first day eggs (1.1 ± 1.4 eggs). Crossing of AP and PS indicated incompatibility in reproduction among the population.

EKSPLORASI DAN IDENTIFIKASI TRICHODERMA ENDOFITIK PADA PISANG Taribuka, Johanna; Sumardiyono, Christanti; Widyastuti, Siti Muslimah; Wibowo, Arif
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 2 (2016): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Exploration and identification of endophytic Trichoderma in banana. Endophytic fungi Trichoderma is an organism that can used as biocontrol agent. This study aims to isolate and identify endophytic Trichoderma in roots of healthy banana plants from three districts in Yogyakarta, which will be used as biological control agent against the pathogen FusariumÂ oxysporum f.sp. cubense. Isolation was conducted using TSM (Trichoderma Selective Medium). We obtained six isolates of endophytic Trichoderma spp., i.e., Swn-1, Swn-2, Ksn, Psr-1, Psr-2, and Psr-3. Molecular identification was done by using ITS1 and ITS2 primer pain and sequenced. The sequence of DNA obtained was analysed and compared with NCBI database by using BLAST-N programe. The results showed that all isolates were amplified at 560-bp. Phylogenetic analysis showed that isolates Swn-1, Swn-2 and Psr-1 are homologous to Trichoderma harzianum, isolate Ksn homologous to TrichodermaÂ asperrellum, isolate Psr-2 homologous to Trichoderma gamsii, and isolate Psr-3 homologous to Trichoderma koningiopsis,Â with the homologous value of 99%.

Integration of Botanical Pesticide and Entomopathogenic Fungi to Control the Brown Stink Bug Riptortus linearis F. (Hemiptera: Alydidae) in Soybean Suharsono .; Yusmani Prayogo
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 1 (2014): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

ABSTRACTIntegration of botanical pesticide and entomopathogenic fungi to control the brown stink bug Riptortus linearis F. (Hemiptera: Alydidae) in soybean. The efficacy of botanical pesticides i.e. Annona squamosa seed powder (ASP) or Jatropha curcas seed powder (JSP) integrated with entomopathogenic fungi, Lecanicillium lecanii to control brown stink bug, Riptortus linearis F. was studied at Muneng Research Station, the Indonesian Legume and Tuber Crops Research Institute (ILETRI) in June up to September 2011. The purpose of the research was to determine the efficacy of integration the natural pesticides i.e, A. squamosa seed powder (ASP) and Jatropha curcas seed powder (JSP) and entomopathogenic fungi L. lecanii to control brown stink bug. The treatments were application of (1) ASP 50 g/l, (2) JSP 50 g/l, (3) ASP 50 g/l + L. lecanii 107/ml, (4) JSP 50 g/l + L. lecanii 107/ml, (5) L. lecanii 107/ml, (6) deltametrin and (7) control (untreated). Application of ASP and JSP combined with L. lecanii decreased the hatched eggs by 84% and 82%, respectively. However, reduction of hactched egg from a single application of ASP and JSP were 56-61% . ASP and JSP combined with L. lecanii reduced both nymphs and adult stink bug population built up and damage (empty pod). Application of ASP and JSP in combination with L. lecanii did not affects the survival of generalist predators such as Paederus sp., Oxyopes sp. and Coccinella sp. as opposed to chemical insecticide that did. The integration of ASP or JSP with entomopthogenic fungi L. lecanii was able to increase the efficacy of brown stink bug control.

EFEK BAKTERI PELARUT FOSFAT TERHADAP PERTUMBUHAN ASPERGILLUS FLAVUS PADA PERKECAMBAHAN KACANG TANAH Sholeh Avivi, Ida Sugeng Suyani & Sugeng Winarso .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 10 No. 1 (2010): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Effect of phosphate solubilizing microorganism to Aspergillus flavus development on peanut germination. Phosphate solubilizing microorganisms (PSM) are microorganisms which could increase the available P in plant media. The aim of this research is to determine the effect of PSM on A. flavus growth, and to identify it’s effects on the growth dynamics of A. flavus as long as the peanut germination. The research were conducted in the Laboratory of Plant Diseases Department, Faculty of Agriculture, University of Jember, from August 2005 to May 2006 and divided in 2 stages: in vitro and germination stage. Base on in-vitro stage we concluded that Bacillus subtilis strain SK had better inhibition toward the growth of A. flavus than Pseudomonas fluorescent strain GM. B. subtilis strain SK could inhibit A. flavus as much as 74%, whereas P. fluorescent strain GM was only reached 60% compared to control. Base on the germination stage, B. subtilis in the desiccator supernatant (BsDS) could increase the dry weight of root up to 24.1 g better than P. fluorescens strain GM and control. We also found that B. subtilis strain SK was more effective to change the availability of P on plant media.

EARLY DETECTION OF TRICHOGRAMMA CHILONIS SEXES USING THE EGG COLOR AND SIZE OF ITS FACTITIOUS HOST, CORCYRA CEPHALONICA F.X. Susilo, Desta S. Romli, Sunaryo, and M. Solikhin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 7 No. 1 (2007): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Early Detection of Trichogramma chilonis sexes using the egg color and size of its factitious host Corcyra cephalonica. This study was aimed to detect the sexes of Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) prior to their emergence using the egg color and size of their factitious host, Corcyra cephalonica (Lepidoptera: Pyralidae). A gray scale of 1 – 7 was used to rank the egg color while a micrometer was used to observe the egg size under a dissecting microscope. Ten of newly laid C. cephalonica eggs were glued in two rows (0.5 cm x 0.4 cm distance) on a piece of oviposition paper. Five replications of such batches were exposed in a test tube to a gravid T. chilonis female for 24 hours under 100 watts of illumination. Following the withdrawal from the oviposition arena the eggs were each observed daily for their color and size and the sex of the emerged T. chilonis progeny was documented. Binomial Z-test at 0.05 level of significance was used to validate the predicted proportion of T. chilonis sexes from five batches of 50 parasitized C. cephalonica eggs. The results showed as predicted that more T. chilonis female progeny emerged from light-colored C. cephalonica eggs (scale 2 – 5) following oviposition by parent parasitoid or from larger C. cephalonica eggs (> 500 µm).

PENAPISAN LIMBAH PERTANIAN (SABUT KELAPA DAN ARANG SEKAM) DALAM PENINGKATAN KETAHANAN BIBIT PISANG BARANGAN BERMIKORIZA TERHADAP BLOOD DISEASE BACTERIUM DAN FUSARIUM OXYSPORUM F.SP. CUBENSE Suswati .; Asmah Indrawati; Deddi Prima Putra
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 15 No. 1 (2015): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Agricultural waste screening (coconut fibre and chaff charcoal) in improving the resistance of Mychorrizae Barangan seedling to Blood diseases bacterium and Fusarium oxysporum f. sp. cubense. The application of soil and compost are very general in Barangan banana seedling. However, those media always contaminated by BDB and Foc propagul. This research was intended to examine the influence of planting media composition (soil, coconut fibre and chuff charcoal) in improving the resistance of Mychorrizae Barangan banana seedling to blood diseases bacterium dan Fusarium oxysporum f sp.cubense. Some experiments conducted in wirehouse using a randomized complete block design application of two subtracts for soil substitution included to either coconut fibre (A) or chuff charcoal (B) (v:v) completed by 6 treatments of each: A0 = 100% soil media, A1 = 50% soil + 50% chuff charcoal, A2 = 50% soil + 25% chuff charcoal + 25% sand, A3 = 25% soil + 50% chuff charcoal + 25% sand; A4 = 75% chuff charcoal + 25% sand, A5 = 100% chuff charcoal, B0 = 100% soil, B1 = 50% soil + 50 % chuff charcoal; B2 = 50% soil + 25 % coconut fiber + 25% sand, B3 = 25% soil +50% coconut fiber +25% sand; B4 = 75% coconut fiber + 25% sand, B5 = 100% coconut fiber. The soil generated from banana seedling area of Sempakata village that seriously infected BDB and Foc. The observation variables encompassed percentage of disease attack, density of BDB and Foc. population, period of pathogen incubation and measurement of Barangan seed and AMF colonization resistance development. The results indicated the planting of Mychorrizae Barangan banana seeds applied diminishing soil media as much as 25â€“100% substituted by chuff charcoal or coconut fiber increased the seed resistance of BDB and Foc.

ASAI ISOLAT BAKTERI KITINOLITIK BACILLUS SP. BK17 PADA MEDIA PEMBAWA TANAH GAMBUT DAN KOMPOS JANJANG KELAPA SAWIT DALAM MENGHAMBAT PERTUMBUHAN JAMUR PATOGEN SCLEROTIUM ROLFSII DAN FUSARIUM OXYSPORUM PADA KECAMBAH CABAI Hutauruk, Deswidya; Suryanto, Dwi; Munir, Erman
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA

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Assay of chitinolytic bacterial isolate of Bacillus sp. Bk17 in peat and palm oil bunch compost as carrier media inÂ inhibiting Sclerotium rolfsii and Fusarium oxysporum of chilli seedlings. Sclerotium rolfsii and Fusarium oxysporum have been known as causal agents of seedling-off of chilli. Biological control has been used as an alternative control to replace chemical control. This study was aimed to determine the viability and ability of chitinolityc bacteria Bacillus sp. BK17 in carrier media of peat and palm oil bunch compost and in growing media to control seedling-off caused by S. rolfsii dan F.Â oxysporum of chilli. Our previous study showed that Bacillus sp. BK17 could reduce disease severity and intensity. Bacterial viability was measured as colony number grown after 90 days of storage in minimum salt medium with colloidal chitin as sole C source. Reduction of disease infection was measured as seedling number infected by S. rolfsii dan F. oxysporum. Seedling performances were measured as seedling height, leaf number and dry-weight after 30-days of growth. The result showed that bacterial cell viability was still high in both peat and palm oil bunch compost both with and without colloidal chitin addition after 90 days of storage. It was also shown that during application bacterial cell could grow. Seedling performaces i.e. seedling height, leaf number and dry-weight showed to be normal or even increase compared to those of pathogenic fungal inoculation only and (-) control.

SERANGAN DACUS CUCURBITAE ( DIPTERA: TRYPETIDAE) PADA BUAH MENTIMUN DAN PARE YANG DIBUNGKUS PADA SAAT PENTIL I Gede Swibawa, F.X. Susilo, Indra Murti, dan Esti Ristiyani .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 3 No. 2 (2003): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Dacus cucurbitae ( Diptera: Trypetidae) attacks on cucumber and peria fruits wrapped at cherelle stage. The fruit flies were important pests of cucumber (Cucumis sativus L) and peria (Memordica charantia L.). The flies attacked fruits by laying egg on the fruit surface and their developing immatures caused serious damages from the inside. The objective of this experiment was to determine the effect of the fruit wrapping at cherelle stage on fruit fly infestation on cucumber or peria fruits. The experiment was conducted in villages of Terbanggi Besar (Central Lampung) and Jati Agung (South Lampung) during October—November 2001. A set of cherelle stage fruits, i.e. those just shed off their florescence, were wrapped using plastic bags while another set of adjacent fruits were left unwrapped. Fruits (treated or untreated) were taken soon after they fell off or at their normal harvest time, whichever came first, then were weighed and incubated in the laboratory to observe the fruit fly emergence. The results showed that fruit wrapping significantly reduced the fruit fly infestation and suppressed the fruit fly population on cucumber or peria fruits. The wrapped fruits weighed more than unwrapped fruits

PENGARUH BEBERAPA EKSTRAK TUMBUHAN TERHADAP DEIGHTONIELLA TORULOSA SYD. ELLIS PENYEBAB PENYAKIT UJUNG HITAM BUAH PISANG Joko Prasetyo dan Efri .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 1 No. 2 (2001): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The effect of some plant extracts on Deightoniella torulosa Syd. Ellis the causative agent of black tip disease of banana. An experiment was conducted to evaluate the effect of some plant extracts on the growth and reproduction of Deightonella torulosa. The plants used in this experiments were Zingiber cassumunar, Acorus calamus, and Amomum cardamomum. The treatments of the factorial (3x4) experiment were arranged in a completely randomized design with three replicates. The first factor (plant extracts) consisted of Z. cassumunar, A. calamus, and A. cardamomum extracts. The second factor (extract concentration) consisted of 0, 100, 200, and 300 mg/l. Variables examined were the colony diameter and conidial density of D. torulosa. The result of the experiment shows that extract of Z. cassumunar, A. calamus, and A. cardamomum extracts suppressed fungal colony diameter. On Z. cassumunar and A. calamus extracts, the higher the concentration the higher fungal colony diameter suppressed. The most effective suppression by the extract was at 300 mg/l. A. calamus extract was more effective than Z. cassumunar and A. cardamomum at 100 mg/ml. Z. cassumunar and A. calamus extracts had the same effectivity at 200 mg/l, and both was more effective compared to A. cardamomum. Z. cassumunar was more effective compared to A. calamus and A. cardamomum at 300 mg/l. The results of the experiment also showed that Z. cassumunar, A. calamus, and A. cardamomum suppressed conidial production of D. torulosa. Z. cassumunar most effectively suppressed conidia production at 300 mg/l. A. calamus extract suppressed conidial production the most effectively at 200 and 300 mg/l. A. cardamomum extract significantly suppressed conidia production only at 300 mg/l. Z. cassumunar and A. calamus extracts were more effective to suppress conidia production compared to A. cardamomum for all level of concentration except control. The effectivity of Z. cassumunar and A. calamus was the same at 100 and 300 mg/l. Z. cassumunar extract was more effective than A. calamus at 200 mg/l.

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