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Dwi Kesuma Sari
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Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research)
Published by Universitas Hasanuddin
ISSN : 26140187     EISSN : 26152835     DOI : -
Core Subject : Health, Agriculture,
Agriculture, Biological Sciences & Forestry Veterinary
Jurnal Riset Veteriner Indonesia (Journal of the Indonesian Veterinary Research) is an open access, double blind peer-reviewed, print and online journal that publishes in the form of original research, review, case study, short communication in any areas of sciences of veterinary, animal sciences, biomedical sciences, biotechnology, biology, animal laboratory and biodiversity of animals. Manuscript should be produced from latest research or study.
Arjuna Subject : Profesi Kesehatan - Profesi Kesehatan (Lain-Lain)
Articles 8 Documents
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Search results for , issue "VOLUME 6 No.1, JANUARY 2022" : 8 Documents clear
The Effect of Sterilization on Vitamin D Levels in Male and Female Rats Pratiwi, Hamria; Idris, Irfan; Sari, Dwi Kesuma
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

Sterilization is an operation that can prevent reproduction and is useful as a treatment. Sterilization is done by removing the reproductive organs, in males it is called orchiectomy and in females it is called ovariohysterectomy which causes a decrease in the production of reproductive hormones. Previous studies have proven a physiologic relationship between reproductive hormones and vitamin D activation, but there is no information on the effect of sterilization on vitamin D levels. This study aimed to determine the effect of sterilization on vitamin D levels. This study was experimental with a Posttest-Only Control Group design. design. A total of 24 wistar rats consisting of 12 male rats and 12 female rats and each were divided into two groups, 6 female rats sterilized (ovariohysterectomy), 6 female rats non-sterilized, 6 male rats sterilized (orchiectomy) and 6 male rats non-sterilized. -sterilization. Wistar rats that were 10 weeks old were transferred to their respective cages and given regular feed to all groups of rats for 7 days as an adaptation process, after which sterilization was carried out in the sterilization group. After 6 weeks post-treatment, the rats were 17 weeks old and then blood was taken for examination of Vitamin D levels using the elisa method which was carried out at Hum-Rc Hasanuddin University Hospital. The results showed that the mean value of vitamin D levels in the ovariohysterectomy group was lower than in the non-sterilized group and was significant (Mann-Withney, p<0.05). The orchiectomy group was also lower than in the non-sterlized group and not significant (Independent T test, p> 0.05). It can be concluded that sterilization can reduce vitamin D levels in pets. Keywords: Sterilization, Ovariohysterectomy, Orchiectomy, and Vitamin D
The Effect of Garlic Extract on the Average Number of Lecocytes in Mice Infected with T. Evansi Kholilullah, Zainal Abidin; Ris, Adryani; Istiana, Nurul; Multazam, Muhammad; Yuliani, Mus Hilda
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

Trypanosomiasis is one of the most widespread diseases in the world. This disease is an infectious disease that often occurs in various types of livestock, especially horses, buffalo and cattle. Trypanosomiasis can cause weakness in infected animals, which can end in death in these animals. This disease is caused by infection with the blood parasite Trypanosoma evansi (T. evansi). The activity of this parasite is very fast. Shortly after entering the animal's body, T. evansi immediately enters the circulatory system and performs breeding activities that can cause damage to blood cells (Ressang, 1984; Wayan et.al 2013). Treatment of trypanosomiasis is currently still using commercial synthetic drugs which are quite expensive, and it has even been found that some substances are no longer effectively used to kill T. evansi. Treatment using basic ingredients derived from plants can be an alternative that needs attention. Garlic bulbs are known to have potential as anti-microbial agents. Its ability to inhibit microbial growth is very broad, including viruses, bacteria, protozoa, and fungi (Nok et al., 1996; Zhang, 1999; Pizorno and Murray, 2000; Yin et al., 2002). Garlic (Allium sativum L) has been widely studied and the results are known to contain active compounds including flavonoids and allicin which have antibacterial, anti-parasitic and antiviral effects (Londhe, 2011). The aim of this study was to determine the effect of giving garlic extract on the average number of white blood cells of mice infected with T. evansi. The study was conducted on thirty male mice of the DDY strain with a body weight of 25-30 g per head which were divided into six treatment groups. Each treatment used five mice as replicates, namely: the untreated group, as a normal control (K0); the group infected with T. evansi intraperitoneally at a dose of 103/0.3 mL; divided into several groups, namely those who were not given any treatment (K1, negative control), the group that was given trypanocidal (K2, positive control), the group that was given garlic extract at a dose of 50 mg/kgBW (K3); the group given garlic extract at a dose of 100 mg/kgBW (K4) and the group given garlic extract at a dose of 200 mg/kgBW (K5). In this study, it was shown that at the beginning of T. evansi infection it caused an increase in the average number of leukocytes in the group of mice that did not receive treatment (K1) and those given trypanocidal (K2), while the group of mice that received treatment with garlic extract showed a decrease in the number the mean leukocyte count (K3 and K4) or a non-significant increase in the mean leukocyte count (P<0.05) at K5.
Mapping of the Coronavirus Circulating in Asia Based on Sequence of Gene Spike and Membrane Protein Used MEGA-X Aplication Rell, Fedri; Jalil, Muhammad Fikri Raditya; Husada, Anggini Putri; Dariming, Nur Azizah
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

Coronavirus are viruses that can be transmitted to human and animals. Severe acute respiratory syndrome, middle east respiratory syndrome, and Coronavirus disease 2019 are disease can be caused by several subtypes of coronavirus.  The aims of this study were to mapping of the coronavirus circulating in Asia based on sequence of gene spike and membrane protein virus. Totally of 67 coronavirus spike protein and membrane gene sequence were accessed via GenBank® (www.ncbi.nlm.nih.gov/genbank/) matched with the ClustalW Method MEGA-X. The result of the study are 20 groups of coronavirus were found based on spike protein gene sequences and 27 groups of coronavirus were found based on membrane protein gene sequences which were different with the first group of coronavirus found in Wuhan. Therefore, it can be concluded that the coronavirus circulate in several Asian countries had been mutate on gene spike and membrane protein. Keywords: Asia, Coronavirus, MEGA-X, Membrane Protein, Spike Protein
The Identification of Toxocara canis on puppy in Makassar Pet Clinic Mustakdir, Zulfikri; Fatmawati, Dian; Sirupang, Meriam
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

Toxocariasis in dogs is caused by infection of Toxocara canis. The disease is not recognized and often underestimated by the owners. Toxocara canis is a pathogenic gastrointestinal worm and the larva could infect the internal organs, which cause diarrhea and kill the animals if the disease is not seriously handled. Toxocara canis is not only danger to the animals but it could also infect the human being.  The purpose of this study was to identification Toxocara canis on puppy in Makassar Pet Clinic. Feces samples were collected from 31 puppies in Makassar Pet Clinic. Feces were examined by native method and floating method to detected eggs of Toxocara canis based on their morphology. The result showed 5 samples were identified positive of Toxocara canis and 26 samples were negative of Toxocara canis Keywords: Toxocariasis, Toxocara canis, Endoparasite, Puppy
Identification and Pathological Finding of Colisepticemia in Broiler Indra, Rusmin; Kardena, I Made; Suarjana, I Gusti Ketut
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

The chicken was sick for 6 days, weak and disrupted growth. Necropsy confirmed that several organs was bleeding and containing fibrin exudation. The results of histopathological examination showed the presence of necrotic encephalitis, hemorrhagic tracheitis, hemorrhagic and necrotic pneumonia, hemorrhagic epicarditis and myocarditis, hemorrhagic enteritis, hemorrhagic and necrotic hepatitis, also hemorrhagic and necrotic glomerulonephritis. Microbiological examination confirmed the discovery of Eschericia coli bacterial agents that can hemolyze blood in the heart, lungs, and intestines. Parasitological examination confirmed that no parasitic agent was involved in this case. From this case study, it can be concluded that the single agent that causes pathological changes was found in the form of pathogenic E. coli bacteria (colisepticemia) which was exacerbated by unfavorable farming conditions. Keywords: Escherichia coli, colisepticemia, broiler, pathological findings
Administration of Live-Attenuated Newcastle Disease (ND) Vaccines Derived from B1 and LaSota Strain and Their Effect on Broiler Antibody Titers Satya Apada, Andi Magfirah; A. Rianti Rhasinta Alifha; Fedri Rell; Trini Purnamasari Syahid
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

Newcastle Disease is a contagious disease caused by Avian Paramyxovirus and infects different types of poultries including broiler. Vaccination as a preventive effort against the ND virus could employ both attenuated and inactivated vaccines. This study aims to identify the effect of administering live-attenuated LaSota and B1 ND vaccines against broiler antibody titers. This experiment employed 54 DOCs and was divided into 3 groups of treatments. Vaccination was performed at the age of 3 days old through eye drop administration. Blood specimens were taken from vena brachialis at the age of 7, 14, 21 days old. Hemagglutination Inhibition (HI) assay was analyzed using One-Way Analysis of Variance (ANOVA) followed by Least Significant Different (LSD) test if the probability is significant (P<0.05). The data of Immune Percentage is presented using descriptive quantitative analysis.   The research results demonstrated that live-attenuated LaSota ND vaccination at the age of 3 days could sustain and induce immunity until the age of 21 days while B1 ND vaccination at the age of 3 days could only sustain immune protection until the age of 14 days. Live-attenuated LaSota and B1 ND vaccines did not have any significant effect on the broiler antibody titers.   Keywords: Broiler, Newcastle Disease (ND), LaSota vaccine, B1 vaccine, Hemagglutination test, Hemagglutination Inhibition Test
The Factors Affecting the Success of Artificial Insemination Program on Cattle in District of Woha, Bima Khoirani, Khuntum; Nikmaturrayan, Nikmaturrayan; HY, Lukman
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

This study aims to determine the factors that affect the success rate of artificial insemination (AI) in Woha district, Bima Regency. This study method was the survey method, using primary and secondary data. Primary data was obtained by distributing questionnaires and interviews directly to farmers as additional information, while secondary data was obtained from inseminators related to the results of artificial insemination in the Woha district. The questionnaire used included questions about cattle characteristics, namely: pregnancy status, type of cattle, age of cattle, body condition score at artificial insemination, number of artificial insemination to pregnant, estrus signs, time implementation of artificial insemination, interval duration of postpartum to estrus, type of straw, insemination doses, reporting interval since lust up to artificial insemination treatment, cattle feed, stocked cattle, and farmer identity with 47 farmer respondents from 3 villages. Data were analyzed using stepwise regression with the help of SPSS. The results showed that of the 90 female cattle carried out by artificial insemination, 85.6% had a pregnancy, and 14.4% had no pregnancy. The independent variable which had the strongest correlation was the age of cows (sig. 0.006), the reporting interval since estrus to artificial insemination treatment (sig. 0.001), and cattle feed (sig. 0.004). The three factors that have the strongest correlation were based on sig. <0.05 so that it had a significant effect on the success of artificial insemination in the Woha district, Bima Regency Keywords: Artificial insemination (AI), beef cattle, succes factor, Woha district    
Anthelmintic Activity of Jatropha (Jatropha curcas linn) Leaf Extract Against Ascaridia galli Worms In Vitro Jamaluddin, Abdul Wahid; Ainun, Hasri; Ris, Adryani; Amir, Muhammad Nur
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, JANUARY 2022
Publisher : Hasanuddin University

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Abstract

Ascariasis is an intestinal worm disease caused by Ascaridia galli. This disease can attack the small intestine and cause a decrease in productivity of local breed chickens. Worm infections in chickens can be suppressed by taking precautions and treatment measures such as improving the management of the cage and providing anthelmintic. Medicinal plants that can be used as an alternative anthelmintic are jatropha leaves (Jatropha curcas linn) which contain secondary metabolites such as alkaloids, saponins, tannins, phenolics, flavonoids, triterpenoids, steroids, and glycosides. The study aims to determine the anthelmintic activity of Jatropha leaf extract against Ascaridia galli worms. 75 samples of Ascaridia galli worms were taken from chicken intestines in traditional markets. This research was conducted in vitro with five treatments consisting of one positive control group using Levamisole, one negative control group with 0.5% NaCMC, and three groups of Jatropha leaf extract (JLE) with concentrations of 10%, 15% and 20 %. The results of the study showed that jatropha leaf extract at a concentration of 20% gave a better anthelmintic effect compared with administration of Levamisole as a positive control (+) and jatropha leaf extract had LC50 and LT50 at concentrations of 5.49% and 96.006 minutes.

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