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Antibacterial Activity of Karanda (Carissa Carandas) Leaf and Fruit Extract to Against Streptococcus mutans, Escherichia coli, Staphylococcus aureus Destik wulandari; Mamik Ponco Rahayu; Fransiska Leviana
Jurnal Kesehatan Farmasi Vol 4, No 1 (2022)
Publisher : Jurusan Farmasi, Poltekkes Kemenkes Palembang

Tanaman karanda (Carissa carandas) memiliki aktivitas antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak daun dan buah karandas sebagai antibakteri terhadap Streptoccocus mutans, Escherichia coli, Staphylococcus aureus. Daun dan buah karandas diekstraksi menggunakan metode maserasi dengan pelarut methanol. Ekstrak daun dan buah karanda diuji aktivitas antibakterinya menggunakan metode difusi seumuran. Hasil penelitian menunjukkan bahwa ekstrak daun Carissa carandas mampu menghambat bakteri S. aureus dan E. coli dan ekstrak buah mampu menghambat bakteri S. mutans dengan optimal.

FORMULASI DAN UJI AKTIVITAS ANTIBAKTERI SEDIAAN SABUN CAIR EKSTRAK SABUT KELAPA (Cocos nucifera L. ) DENGAN VARIASI KONSENTRASI CARBOPOL 940 TERHADAP BAKTERI Staphylococcus aureus ATCC 25923 Febriana Mukti Permata Sari; Siti Aisiyah; Destik Wulandari
EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Vol 4, No 1 (2023)
Publisher : FKIP Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/edunaturalia.v4i1.61425

Shower gel is one of the preparations that can be used to protect the skin from Staphylococcus aureus bacterial infection. Plants that have antibacterial activity are coconut husk, the compounds contained are tannins, flavonoids, and polyphenols. Tannins have the activity of inhibiting the growth of Staphylococcus aureus bacteria. This study aims to determine the influence of Carbopol 940 variations on liquid soap preparations. Liquid soap preparations have antibacterial activity, find out which formulas have the best physical quality. Coconut husk extract was obtained by maceration method using 70% ethanol solvent. The concentration of coconut husk extract in the formula is 1.6% with a variation in the concentration of Carbopol 940 which is 0.5%, 1%, 1.5%. Physical quality examination includes organoleptic test, homogeneity test, pH test, viscosity, foam stability test, and free alkali test. Testing the antibacterial activity of liquid soap preparations using the method of diffusion of wells, the data obtained by analysis using the SPSS program. Variations in carbopol concentration 940 affect the physical quality of liquid soap preparations, the increasing concentration of carbopol affects viscosity, foam stability, and free alkali. All formulas have antibacterial activity of 21.23 (F4); 20.22 (F5); 19.37 (F6). The formula with the best physical quality and inhibitory power is formula 4 with a carbopol concentration of 940 of 0.5%.

Uji Aktivitas Antijamur Fraksi N-Heksana, Etil Asetat, dan Air Ekstrak Metanol Kulit Batang Jambu Mete (Anacardium occidentale L.) Terhadap Candida albicans ATCC 10231 Destik wulandari
Jurnal Kesehatan Farmasi Vol 5, No 1 (2023)
Publisher : Jurusan Farmasi, Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jkpharm.v5i1.1630

The bark of cashew nuts (Anacardium occidentale L.) contains compounds such as alkaloids, flavonoids, tannins and saponins which have the potential to inhibit the growth of fungi. The purpose of this study was to determine the antifungal activity of the extract, the n-hexane fraction, ethyl acetate, water and to determine the Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) of the most active fractions of the methanol extract of cashew nut bark against the fungus Candida albicans ATCC 10231.

A Penetapan Kadar Fenolik Total, Uji Aktivitas Tabir Surya Dan Antibakteri Propionibacterium acnes Dari Ekstrak Etanol Daun Gambir (Uncaria gambir Roxb): Penetapan kadar fenolik total, Uji aktivitas tabir surya dan antibakteri dari ekstrak etanol daun gambir Sumarsono, shoraya anis wulandari; Endang Sri Rejeki; Destik Wulandari
Jurnal Komunitas Farmasi Nasional Vol. 3 No. 02 (2023): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar

Gambir leaves (Uncaria gambir Roxb) contain phenolic compounds that have the potential to act as sunscreen and have antibacterial activity. This study aims to determine the total phenolic content, SPF value of gambier leaf extract in vitro and antibacterial activity against Propionibacterium acnes. Gambier leaf extract was made using the maceration method with 96% ethanol solvent. Total phenolic content and sunscreen activity were tested by measuring absorption using UV-Vis spectrophotometry, while the antibacterial activity test used the agar well method. Data were analyzed using Microsoft Excel to test phenolic levels and SPF values, while for antibacterial activity using SPSS 25 with the One Way Anova test. The results showed that the total phenolic content of gambier leaf extract was 1.3322 mgGAE/g ± 0.2456 with ultra-protective SPF values at concentrations of 120, 100 and 80 ppm respectively, namely 31,94; 22,62; and 15,24. Gambir leaf extract has antibacterial activity against Propionibacterium acnes with inhibitory diameters at concentrations of 50, 25 and 12.5% respectively, namely 23,67; 18,00; and 14,57 mm.

Penetapan Kadar Fenolik Total, Uji Aktivitas Tabir Surya Dan Antibakteri Propionibacterium acnes Dari Ekstrak Etanol Daun Gambir (Uncaria gambir Roxb) Sumarsono, shoraya anis wulandari; Endang Sri Rejeki; Destik Wulandari
Jurnal Komunitas Farmasi Nasional Vol. 4 No. 1 (2024): Jurnal Komunitas Farmasi Nasional
Publisher : Akademi Farmasi Yarsi Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar

Gambir leaves (Uncaria gambir Roxb) contain phenolic compounds that have the potential to act as sunscreen and have antibacterial activity. This study aims to determine the total phenolic content, SPF value of gambier leaf extract in vitro and antibacterial activity against Propionibacterium acnes. Gambier leaf extract was made using the maceration method with 96% ethanol solvent. Total phenolic content and sunscreen activity were tested by measuring absorption using UV-Vis spectrophotometry, while the antibacterial activity test used the agar well method. Data were analyzed using Microsoft Excel to test phenolic levels and SPF values, while for antibacterial activity using SPSS 25 with the One Way Anova test. The results showed that the total phenolic content of gambier leaf extract was 1.3322 mgGAE/g ± 0.2456 with ultra-protective SPF values at concentrations of 120, 100 and 80 ppm respectively, namely 31,94; 22,62; and 15,24. Gambir leaf extract has antibacterial activity against Propionibacterium acnes with inhibitory diameters at concentrations of 50, 25 and 12.5% respectively, namely 23,67; 18,00; and 14,57 mm.

Uji Aktivitas Antibakteri Hasil Fermentasi Bakteri Endofit Umbi Talas (Colocasia esculenta L) terhadap Bakteri Pseudomonas aeruginosa : Potential of Antibacterial Compound Fermentation of Endophytic Bacteria from Taro Tuber (Colocasia esculenta L.) againts Pseudomonas aeruginosa Desi Purwaningsih; Destik Wulandari
Jurnal Sains dan Kesehatan Vol. 3 No. 5 (2021): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v3i5.622

Bacterial endophyte is an endosimbiont which lives within host plans without causing apparent disease on its host plans. Some endophytic bacteria found in plants have the capability to produce potential compounds as antibacterial. This study aimed to determine the antibacterial activity of the endophytic bacteria fermentation from the tubers of taro plants and determine the optimum time of fermentation which produce the highest antibacterial activity. This research was initially started by producing the suspension of the endophytic bacteria and test bacteria. The suspension that had been made then was used to characterize and identify the endophytic bacteria and test bacteria. Identification methods include colony identification, Gram staining, Spore staining and biochemical tests planted on MCH, SIM, LIA and Citrate mediums. The fermentation was then carried out to produce secondary metabolite compounds which were utilized to examine the antibacterial activity against Pseudomonas auriginosa. The antibacterial activity test was conducted by the disk diffusion assay. The results of the antibacterial activity assay showed that Bacillus siamenssis and Bacillis subtilis were both able to produce secondary metabolites that have potential as antibacterial against Pseudomonas earuginosa. The highest value was achieved by 72 hours fermentation time with a value of 8.7 by Bacillus siamensis. The endophytic bacteria Bacillus subtilis had the highest activity value at 48 hours fermentation time with an average of diameter of inhibition zone of 8.97.

Uji Aktivitas Antibakteri Lendir Bekicot (Achatina Fulica) Terhadap Bakteri Escherichia Coli Dan Uji Kadar Total Protein Otu, Larasati Rambu; Iswandi, Iswandi; Wulandari, Destik
PHARMADEMICA : Jurnal Kefarmasian dan Gizi Vol 4 No 1 (2024): April - September
Publisher : LPPM-KI - POLTEKKES PIM (Formerly AKAFARMA-AKFAR PIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54445/pharmademica.v4i1.54

Snails (Achatina fulica) have mucus whose main contents include beta agglutinin, Achasin protein, oacharan sulfate and glycoconjugates. Snail mucus can also treat wound infections in the digestive tract and surgical site infections (SSI), one of which is caused by Escherichia coli bacteria. This research utilizes the antibacterial content in snail mucus to inhibit Escherichia coli bacterial colonies and determine the total protein levels in snail mucus. The antibacterial activity test used the disk diffusion method and analysis of total protein levels in snail mucus was analyzed using the biuret method with a UV-Vis spectrophotometer and albumin as a standard solution. The research results were entered in tabular data and analyzed descriptively. The results of quantitative tests on the antibacterial activity of snail mucus showed that there was antibacterial activity of snail mucus against Gram negative Escherichia coli bacteria with concentrations of 100%, 50% and 25% having clear zones of 14, 11.88 and 8.66 mm respectively. The results of calculating the total protein content using the UV-Vis Spectrophotometer method were 11%. This test produced a high total protein content

UJI AKTIVITAS ANTIBAKTERI DAN ANTIBIOFILM EKSTRAK ETANOL DAUN MATOA (Pometia pinnata J. R & G. Forst) TERHADAP BAKTERI Pseudomonas aeruginosa Putri, Seprina; Indrayati, Ana; Wulandari, Destik
EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Vol 6, No 1 (2025)
Publisher : FKIP Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/edunaturalia.v6i1.92502

Pseudomonas aeruginosa is a multi-drug resistant (MDR) pathogen known for its ability to form biofilms, making it difficult to eliminate. This study aimed to evaluate the effectiveness of ethanol extract from matoa leaves (Pometia pinnata) as an antibacterial and antibiofilm agent against P. aeruginosa. The antibiofilm activity was assessed using the Crystal Violet (CV) method with absorbance measured at a wavelength of 595 nm. The percentage of biofilm inhibition was used to determine the ICâ‚…â‚€ value. Phytochemical analysis revealed that the ethanol extract of matoa leaves contains alkaloids, flavonoids, tannins, and triterpenoids, which contribute to its antibacterial and antibiofilm activity. The minimum inhibitory concentration (MIC) was determined to be 25 mg/mL. Inhibition zone tests showed that the ethanol extract at concentrations of 75, 50, and 25 mg/mL produced inhibition zones of 13.33 mm, 12.16 mm, and 10.8 mm, respectively, while the positive control, ciprofloxacin 5 µg, produced an inhibition zone of 35.67 mm. The percentages of biofilm inhibition were 21.51% (50 mg/mL) and 12.34% (25 mg/mL), with an average ICâ‚…â‚€ value of 27.43 mg/mL. These findings suggest that the ethanol extract of matoa leaves has potential as an antibacterial and antibiofilm agent against P. aeruginosa. Keywords: Pseudomonas aeruginosa, Antibacterial, Antibiofilm, Matoa leaves

Formulasi Dan Uji Aktivitas Antibakteri Sediaan Sabun Cair Ekstrak Bunga Cengkeh (Syzygium aromaticum L.) Dengan Variasi Konsentrasi Carbopol 940 Terhadap Bakteri Staphylococcus aureus ATCC 25923 Khatimah, Husnul; Aisiyah, Siti; Wulandari, Destik
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 3 No. 1 (2023): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v3i1.16609

Liquid soap is one of the preparations to prevent skin infections caused by bacteria. One of the bacteria that causes infection is Staphylococcus aureus. The content of flavonoids, tannins and alkaloids found in clove flowers (Syzygium aromaticum L.) can inhibit antibacterial activity. This study aims to determine the effect of varying concentrations of carbopol 940 on the physical quality of liquid soap preparations of clove flower extract, having antibacterial activity against Staphylococcus aureus bacteria and which formula has the best physical quality. The results showed that variations in carbopol 940 affected the physical quality of the liquid soap preparation, i.e. the increasing concentration of carbopol 940 affected the viscosity and free alkali. All formulas have antibacterial activity of 26.27 (F4); 25.90 (F5); 25.52 (F6). The formula with the best physical quality and antibacterial activity was formula 4 with a concentration of 0.5% carbopol 940 and had an inhibitory effect on bacteria of 26.27 mm. AbstrakSabun cair adalah salah satu persiapan untuk mencegah infeksi kulit yang disebabkan oleh bakteri. Salah satu bakteri yang menyebabkan infeksi adalah Staphylococcus aureus. Kandungan flavonoid, tanin, dan alkaloid yang ditemukan dalam bunga cengkeh (Syzygium aromaticum L.) dapat menghambat aktivitas antibakteri. Penelitian ini bertujuan untuk menentukan efek variasi konsentrasi carbopol 940 pada kualitas fisik dari persiapan sabun cair ekstrak bunga cengkeh, yang memiliki aktivitas antibakteri terhadap bakteri Staphylococcus aureus, dan formula mana yang memiliki kualitas fisik terbaik. Hasil penelitian menunjukkan bahwa variasi carbopol 940 mempengaruhi kualitas fisik dari persiapan sabun cair, yaitu peningkatan konsentrasi carbopol 940 mempengaruhi viskositas dan alkali bebas. Semua formula memiliki aktivitas antibakteri sebesar 26,27 (F4); 25,90 (F5); 25,52 (F6). Formula dengan kualitas fisik dan aktivitas antibakteri terbaik adalah formula 4 dengan konsentrasi carbopol 940 0,5% dan memiliki efek penghambatan bakteri sebesar 26,27 mm.

Uji Aktivitas Antibakteri Sediaan Obat Kumur Ekstrak Daun Mimba (Azadirachta indica A. Juss) terhadap Bakteri Streptococcus mutans ATCC 25175 Bila, Nabila Salsa; Fadma Sari, Ghani Nurfiana; Wulandari, Destik
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 4 No. 1 (2024): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/organisms.v4i1.22196

Mouthwash is a preparation in liquid form and contains antibacterial substances. Neem leaves contain a class of compounds in the form of steroids/triterpenoids which are useful for antibacterial activity so they have the potential to be used as active substances in mouthwash preparations. The aim of this research was to determine the effect of variations in glycerin concentration on the physical quality and stability of neem leaf extract (Azadirachta indica A. Juss) mouthwash preparations. To determine the antibacterial effect and formula for mouthwash from neem leaf extract which has the most effective antibacterial effect against the bacteria Streptococcus mutans. This research uses four formulations for mouthwash from neem leaf extract (Azadirachta indica A. Juss) at 1% with variations in glycerin concentration of F1 (12%), F2 (24%), F3 (30%). Physical quality testing of the preparation includes organoleptic, homogeneity, specific gravity, pH, viscosity and stability of the preparation (cycling test). Mouthwash preparations were tested against Streptococcus mutans bacteria using the paper disc diffusion method. The research results showed that mouthwash preparations in all formulas with organoplastic physical quality, homogeneity, viscosity, pH, specific gravity and stability produced good preparations. In testing the antibacterial activity of mouthwash preparations, the diameter of the inhibition zone was obtained, namely F1 9.94 mm; F2 11.57mm; F3 8.91mm; F- (k-) 0 mm; and positive control 9.47 mm. From the third formula, the one with the best inhibition zone diameter is F2. AbstrakObat kumur merupakan sediaan yang berbentuk cairan dan memiliki kandungan zat antibakteri. Daun mimba memiliki kandungan golongan senyawa berupa steroid/triterpenoid yang bermanfaat sebagai aktivitas antibakteri sehingga berpotensi untuk dijadika zat aktif pada sediaan obat kumur. Tujuan dari penelitian ini untuk mengetahui pengaruh variasi konsentrasi gliserin terhadap mutu fisik dan stabilitas sediaan obat kumur ekstrak daun mimba (Azadirachta indica A. Juss). Mengetahui efek antibakteri dan formula obat kumur ekstrak daun mimba yang paling efektif efek antibateri terhadap bakteri Streptococcus mutans.Penelitian ini menggunakan empat formula sediaan obat kumur ekstrak daun mimba (Azadirachta indica A. Juss) sebesar 1% dengan variasi konsentrasi gliserin sebesar F1 (12%), F2 (24%), F3 (30%).Pengujian mutu fisik sediaan meliputi organoleptis, homogenitas, berat jenis, pH, viskositas, dan stabilitas sediaan (cycling test). Sediaan obat kumur diujikan terhadap bakteri Streptococcus mutans menggunakan metode difusi cakram kertas. Hasil penelitian menunjukkan bahwa sediaan obat kumur pada semua formula dengan mutu fisik organoplik, homogenitas, viskositas, pH, berat jenis dan stabilitas menghasilkan sediaan yang baik. Pada pengujian aktivitas antibakteri sediaan obat kumur didapatkan nilai diameter zona hambat yaitu F1 9,94 mm; F2 11,57 mm; F3 8,91 mm; F- (k-) 0 mm; dan kontrol positif 9,47 mm. Dari ketiga formula yang memiliki diameter zona hambat paling baik yaitu F2.

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