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All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Farmasi Indonesia INTEGRITAS : Jurnal Pengabdian Pharmademica: Jurnal Kefarmasian dan Gizi EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Journal Prima Health Science Jurnal Kesehatan Farmasi Herbal Medicine Journal Organisms: Journal of Biosciences Jurnal Komunitas Farmasi Nasional Jurnal Sains dan Kesehatan Jurnal Farmasi Ikifa
Wulandari, Destik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Veterinary

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Isolasi dan Uji Aktivitas Enzim Superoksida Dismutase (SOD) dari Bakteri Endofita Daun Tanaman Sukun (Artocarpus altilis) dengan Metode Water Soluble Tetrazolium Salt-1 (Wst-1) Fradine, Chossy; Wulandari, Destik; Purwaningsih, Desi
Herbal Medicine Journal Vol 8 No 2 (2025): Herbal Medicine Journal
Publisher : Program Studi S1 Farmasi, STIKES Senior, Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58996/hmj.v8i2.166

Abstract

Endophytic bacteria are bacteria that carry out symbiosis with host plants without causing disease without the host plant. Some endophytic bacteria found in plants are able to produce potential compounds as antioxidants by producing the enzyme SOD (Super Oxide Dismutase). This research aims to determine the results of endophytic bacterial fermentation from the leaves of the breadfruit plant (Artocarpus altilis). This research began with the identification of endophytic bacteria, then continued with the extraction of the SOD enzyme by adding PBS and centrifuging. Purification of the SOD enzyme was carried out using the ammonium sulfate precipitation method. Determination of total protein content using the Lowry method. SOD activity was measured by measuring the percent inhibition value using the Superoxide Dismutase Activity Assay Kit WST-1. The results of the research showed that the isolated bacteria had morphological characteristics in sequence from colony shape, color, size, elevation, edges, cell shape and Gram type for AL 4 bacteria Filamentous, white, medium, flat, Rhizoid, coccus, Gram +, citrate -, Catalase -, for IL11 bacteria Irregular, white, large, flat, entire, bacilli, and Gram +, citrate +, Catalase +. The extraction results from the crude extract produced a sediment weight of 2,915 g for AL4 and 1,653 g for IL11. The total protein content of the crude extract and 30% ammonium sulfate precipitation for isolate AL4 was 1.74 mg/mL and IL11 1.79 mg/ml. Then the percent inhibition value for isolate AL4 with a concentration of 20% was 64.02% and a concentration of 80% was 71.43%.
UJI AKTIVITAS ANTIBAKTERI FRAKSI EKSTRAK DAUN KERSEN (Muntingia calabura L.) TERHADAP BAKTERI Staphylococcus aureus Brigita Aguilera Evarlin Jeharum; Iswandi; Destik Wulandari
Jurnal Farmasi IKIFA Vol. 4 No. 3 (2025): JURNAL FARMASI IKIFA VOL. 4 NO. 3 OKTOBER TAHUN 2025
Publisher : Sekolah Tinggi Ilmu Kesehatan IKIFA

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Abstract

Staphylococcus aureus is a gram-positive bacterium that is part of the normal flora of human skin and mucous membranes. Staphylococcus aureus can cause infectious diseases. Cherry leaves are known to contain active compounds with antibacterial potential. The purpose of this study was to determine the antibacterial activity of cherry leaf extract fractions against Staphylococcus aureus bacteria, determine the MIC and MBC values, and detect the active fraction content. This study used an extraction method by maceration using 70% ethanol solvent and fractionation of cherry leaf extract using n-hexane, ethyl acetate, and water solvents. The disk diffusion method used a dilution series of 50%; 25%; 12.5% to obtain the most active fraction activity. The dilution method to determine the effectiveness of the concentration of the diffusion test with a dilution series of 50%; 25%; 12.5%; 6.25%; 3.12%; 1.56%; 0.78%; 0.39%. Data in the diffusion test were analyzed using SPSS to obtain the best activity. The normality test used the Shapiro Wilk test and homogeneity with Levene Statistic, then continued with the One Way ANOVA test. The results of the study showed that the most active extract fraction tested by the diffusion method was in the ethyl acetate fraction with a diameter of 26.95 mm. The ethyl acetate fraction tested by the dilution method had a MBC value of 6.25%. The ethyl acetate fraction was tested for content detection by Thin Layer Chromatography containing flavonoids, tannins, saponins, and alkaloids.
Co-Authors Aji Wahyu Wahiddatul Djakiah Ana Indrayati Ayu Rachmawati Bila, Nabila Salsa Brigita Aguilera Evarlin Jeharum Desi Purwaningsih Endang Sri Rejeki Febriana Mukti Permata Sari Fradine, Chossy Fransiska Leviana Ghani Nurfiana Fadma Sari Husnul Khatimah Husnul Khatimah Isna Jati Asih Isna Jati Asiyah Isna Jati Asiyah Iswandi Iswandi Iswandi Iswandi Mamik Ponco Rahayu Otu, Larasati Rambu Putri, Seprina Siti Aisiyah Siti Aisiyah Siti Aisiyah Sumarsono, shoraya anis wulandari Yoga Alim Prisnanda