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All Journal EKSAKTA: Journal of Sciences and Data Analysis Jurnal Ilmiah Pertanian Dharma Bakti JKPK (Jurnal Kimia dan Pendidikan Kimia) IJCR (Indonesian Journal of Chemical Research)
Fajarwati, Febi Indah
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Materials Science & Nanotechnology Social Sciences Other

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Production of Protease Enzymes and Bioactive Compounds by Lactic Acid Bacteria (LAB) From Broccoli (Brassica oleracea L.) Using Lactobacillus plantarum Starter Hidayat, Habibi; Maharani, Najlaa Ayu; Maulani, Qisti; Fajarwati, Febi Indah
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 7, ISSUE 1, April 2026
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol7.iss1.art12

Abstract

This study aimed to isolate and characterize Lactic Acid Bacteria (LAB) from broccoli (Brassica oleracea L.) with potential protease-producing activity and probiotic properties. Fermentation was carried out using Lactobacillus plantarum as a starter culture. The obtained LAB isolates were subsequently evaluated for protease activity and analyzed for bioactive compound content. The results demonstrated that LAB isolates obtained from broccoli were capable of producing proteases with significant activity, indicating their potential for biotechnological applications. In addition, the isolates produced bioactive compounds exhibiting antibacterial activity against several pathogenic bacteria, including S. epidermidis, K. pneumoniae, E. coli, S. aureus, S. typhi, and S. pyogenes, as evidenced by the formation of inhibition zones. The isolate demonstrated strong antibacterial activity against all tested pathogens with inhibition zones of 56 mm for S. epidermidis, 30 mm for S. typhi, 12 mm for both K. pneumoniae and E. coli, and 11 mm for S. pyogenes and S. aureus. Acid tolerance assays demonstrated that the LAB isolates survived at low pH, and protease activity assays showed that the isolate produced an enzyme with proteolytic activity of 23.5 mm and 0.0440 U/mL.
Improving electrochemical sensor performance for detection of 3-MCPD based on moleculary imprinted polymer-Nd2O3-Graphene Fajarwati, Febi Indah; Fadillah, Ganjar; Hidayat, Rahmat
JKPK (Jurnal Kimia dan Pendidikan Kimia) Vol 10, No 2 (2025): JKPK (Jurnal Kimia dan Pendidikan Kimia)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jkpk.v10i2.106233

Abstract

Palm oil is a key raw material in the food industry with significant strategic value. However, during the refining and deodorization processes, it can produce 3-monochloropropane-1,2-diol (3-MCPD), a compound known to be carcinogenic. Therefore, a detection method with high selectivity and sensitivity is essential. In this study, an electrochemical sensor based on molecularly imprinted polymers (MIPs) was developed and integrated with neodymium oxide–graphene (Nd₂O₃/Gr) nanocomposites. Characterization techniques such as Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) confirmed the successful fabrication and favorable morphology of the MIPs–Nd₂O₃/Gr composites. The sensor’s performance was evaluated using Differential Pulse Voltammetry (DPV) and cyclic voltammetry methods. The incorporation of Nd₂O₃ and graphene significantly enhanced the sensor’s ability to detect 3-MCPD, demonstrating excellent linearity (R² = 0.9932) across a wide concentration range. Moreover, pH was found to play a critical role in low-concentration detection, influencing the sensor’s response. These findings indicate that the MIPs–Nd₂O₃/Gr sensor offers high selectivity and sensitivity, making it a promising candidate for monitoring 3-MCPD levels in food products and enhancing food safety control.
Co-Authors Airun, Nurul Hidayati Beta Wulan Febriana Dwiputri, Adelia Yuni Ganjar Fadillah, Ganjar Habibi Hidayat, Habibi Harry Supriadi Hermawati, Athalia Tasya Ika Yanti, Ika Kesumawati, Ayundyah Krisna Merdekawati, Krisna Maharani, Najlaa Ayu Mai Anugrahwati Matahelumual, Bethy C Maulani, Qisti Mayang Sari, Sukma Radik Muchtar, Khopipah Mulwandari, Meike Mutiara Herawati, Mutiara Nasriyanti, Desi Putri, Annisa Dwi Rahmat Hidayat Safitri, Rika Awalin Salmahaminati Salmahaminati, Salmahaminati Sri Widada Widinda Normalia Arlianty, Widinda Normalia Wiyantoko, Bayu Zukhrufi, Zukhrufi