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PELATIHAN PENGAMATAN KESUBURAN TANAH, PEMANFAATAN ORGANISME TANAH UNTUK PENGENDALI HAMA URET SERTA PERBAIKAN MANAJEMEN LABORATORIUM BERBASIS ISO 17025 Setiawati, Tri Candra; ",", Basuki; Sulistyaningsih, Niken
Jurnal Pengabdian Masyarakat IPTEKS Vol 5, No 2 (2019): JURNAL PENGABDIAN MASYARAKAT IPTEKS
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/jpmi.v5i2.2942

Abstract

SMKN 4 adalah sekolah kejuruan di kabupaten Bondowoso, bidang keahlian untuk siswa yaitu Agribisnis-Agroteknologi. Keahlian Agribisnis-Agroteknologi, menuntut laboratorium yang baik, siswa mempunyai skill kerja praktis di lapang, serta kemampuan mengembangkan sumberdaya alam terutama pengendali patogen tanah. Permasalahan di tempat tersebut penataan laboratorium, dan kegiatan praktek dilapang masih kurang, serta pemahaman potensi pengembangan pengendali hama uret belum ada. Oleh sebab itu, penting dilakukan upaya untuk meningkatka skill siswa. Peningkatan skill dan wawasan dilakukan dengan kegiatan pelatihan yaitu : 1). melaksanakan koordinasi dengan mitra, 2). menghimpun dan membuat materi pelatihan, 3). Sosialisasi tentang manajemen laboratorium sesuai SNI 17025, 4). pelatihan pembuatan intruksi kerja laboatorium sesuai SNI 17025: 2017, 5). Pelatihan Good Laboratiry Practice (GLP), 6). Sosialisasi Pengamatan Kesuburan Tanah secara cepat, 7). Pelatihan Pengamatan Kesuburan Tanah Untuk Tanaman, 8). Sosialisasi Hama Lepidiota stigma Pada Tanaman Tebu, 9). Pelatihan Pembuatan Jamur Metharizium Untuk Pengendali Hama Lepidiota stigma, 10). Pelatihan Packaging Jamur Metharizium. Hasil yang didapat dari pelatihan tersebut, yaitu nilai positif dari siswa tentang pengetahuan dan wawasan baru yang terkait dengan manajemen laboratorium, penentuan kesuburan tanah secara cepat di lapang, dan pembuatan jamur Metharizium sebagai pengendalian hama uret (Lepidiota stigma).
Praktik Pembibitan Dan Revitalisasi Mangrove Guna Mengembangkan Ekoeduwisata Hutan Mangrove di Wilayah Pesisir Desa Agel Kecamatan Jangkar Situbondo Romadhona, Sukron; Mutmainnah, Laily; Setiawati, Tri Candra
Community Empowerment Vol 5 No 2 (2020)
Publisher : Universitas Muhammadiyah Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.797 KB) | DOI: 10.31603/ce.v5i2.4050

Abstract

Mangrove atau di Indonesia biasa disebut bakau adalah nama sekelompok tumbuhan dari marga Rhizophora, suku Rhizophoraceae. Tumbuhan ini memiliki ciri-ciri yang mencolok, yaitu berupa akar tunjang yang besar dan berkayu, pucuk yang tertutup daun penumpu yang meruncing, serta buah yang berkecambah serta berakar ketika masih di pohon (vivipar). Hutan mangrove terdapat di sepanjang garis pantai di kawasan tropis, dan menjadi pendukung berbagai jasa ekosistem, termasuk produksi perikanan dan siklus unsur hara. Namun luas hutan mangrove telah mengalami penurunan sampai 30–50% dalam setengah abad terakhir ini. Termasuk kondisi ekosistem mangrove khususnya di Jawa Timur sebagian besar rusak, sebagai akibat kekurangan pemahaman, kurangnya kepedulian baik masyarakat maupun stakeholder yang terkait. Tujuan dan sasaran tersebut dicapai melalui kegiatan kunjungan ke stakeholder (dinas/instansi) terkait dengan pengembangan dan pengelolaan ekosistem mangrove di wilayah kabupaten yang memiliki kawasan pantai, untuk melakukan pendekatan dan serap informasi mengenai kondisi mangrove dan pengelolaannya, kemudian kita menjalin pendekatan melalui workshop dan praktik pembibitan serta kegiatan penanaman 1500 bibit mangrove di lahan seluas 3 Ha sebagai bagian dari program untuk menciptakan alternatif pendapatan dari sektor pengelolaan lingkungan. Kegiatan pengabdian terutama ditujukan untuk meningkatkan kesadaran dan kepedulian masyarakat di wilayah pesisir terhadap pengembangan dan pengelolaan ekosistem mangrove, dengan target khusus tersusunnya strategi pengembangan dan pengelolaan ekosistem mangrove serta terbentuknya Kelompok Kerja Petani Mangrove di wilayah Desa Agel, Kecamatan Jangkar Kabupaten Situbondo. Harapannya, kedepan masyarakat di wilayah pesisir semakin berdaya memiliki alternatif pendapatan yang sekaligus juga dalam rangka menjaga ekosistem alami di wilayah pesisir dan menjadikan kawasan pesisir khususnya kawasan hutan mangrove nantinya menjadi kawasan ekoeduwisata yang menarik bagi para pengunjung, lebih jauh lagi tujuan inti dari kegiatan pengabdian ini adalah pengembangan dan pengelolaan ekosistem mangrove secara berkelanjutan.
Identifikasi dan Kuantifikasi Metabolit Bakteri Pelarut Fosfat dan Pengaruhnya terhadap Aktivitas Rhizoctonia solani pada Tanaman Kedelai Setiawati, Tri Candra; Mihardja, Paniman Asna
JOURNAL OF TROPICAL SOILS Vol 13, No 3: September 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i3.233-240

Abstract

Phosphate solubilizing bacteria (PSB) metabolites are organic acids, phosphomonoesterase enzyme (alkaline phosphatase) and antibiotic, which is able to dissolve insoluble phosphate. Phosphate solubilizing bacteria used in this study was expected to suppress Rhizoctonia solani attacks. This experiment was aimed at (1)  identifiying and quantifying  PSB metabolites, and (2) examining their capability as biocontrol agent for Rhizoctonia solani in vitro and hydroponics soybean. This study was conducted in three stages. The first stage of this study was culturing two PSB isolates (Pseudomonas putida 27.4B and Pseudomonas diminuta) in the Pikovskaya medium to analyze their metabolites. The second and third stage of this study was testing the antagonist of two bacteria to suppressed R. solani activity, which was conducted in vitro, and in hydroponics medium soybean as indicator plant. The results showed that P. putida 27.4B and P. diminuta produced organic acids i.e.: citrate, formic, succinic, acetic, propionate, butyrate, and oxalate. The totals of organic acids from each bacterium were 70,3 mg.kg-1 and 61,9 mg.kg-1. Production of alkaline phosphatase enzyme in Pikovskaya medium of P. Putida27.4B was 11,71 μg pNP .mL-1.h-1 and P. diminuta was 24,04  μg pNP.mL-1.h-1. Concentration of this enzyme in soil medium was higher than that in Pikovskaya medium with 26,27 μg pNP.g-1.h-1 and 39,03 μg pNP.g-1.h-1 respectively. This study also showed that total concentration of antibiotics (tetracycline, oxitetracycline and penicillin) produced by the PSB, were 3,2 μg.mL-1 (P. putida 27.4B) and 10,96 μg.m1-1 (P. diminuta), respectively. The results from second stage of this study showed that by using in vitro, the reduced growth of  R. solani was observed 58,35% with P. putida 27.4B and 41,96% with P. diminuta. In addition, inoculations of PSB in hydroponics medium reduced the fungal pathogenesis from 10,71% to 21,42% of pre and post emergence damping-off. Visually, the symptom of pathogen attack appeared within the period of  2 untill 14 days after infection.
Pemetaan Prediksi Erosi Tanah pada Litologi Breksi Argopura Lereng Gunung Argopura dengan Pendekatan Survai Menggunakan Metode USLE dan SIG Basuki, Basuki; Saputra, Tri Wahyu; Mutmainnah, Laily; Setiawati, Tri Candra; Mandala, Marga; Sari, Vega Kartika
Jurnal Ilmiah Rekayasa Pertanian dan Biosistem Vol 12 No 2 (2024): Jurnal Ilmiah Rekayasa Pertanian dan Biosistem
Publisher : Fakultas Teknologi Pangan & Agroindustri (Fatepa) Universitas Mataram dan Perhimpunan Teknik Pertanian (PERTETA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jrpb.v12i2.583

Abstract

Soil erosion is the loss of land or part of the land from one place to another due to water and wind factors. Land use on the slopes of Mount Argopura has been partly converted to seasonal crops and plantation crops, especially sugar cane. There have been changes, such as in the rainy season, when water flowing into the downstream areas is murky, carrying thick mud. This condition indicates that soil erosion is occurring. The aim of the research is to assess and predict the hazard level of soil erosion in mountainous area of Argopura with the lithology of the Argopura breccia by using the Universal Soil Loss Equation (USLE) method and geographic information systems (GIS). The results showed that the  mountainous area of Argopura is mostly covered by the heavy category area, with soil loss of 180–480 tons/ha/year and an area distribution reaching 1,502.49 ha (37.59%). The distribution in the heavy category was found in land use type of moorland and paddy fields, both irrigated and rainfed. The potential for soil erosion in the very heavy category with a soil loss rate of >480 tons/ha/year covers an area of 508.52 ha (12.72%). The very heavy category is partly due to the use of jungle land and steep slopes, so cultivation carried out on this land needs to be replaced with plant conservation, which can reduce soil erosion.
Elemental composition and mineralogical characteristics of volcanic ash and soil affected by the eruption of Mount Semeru, East Java Setiawati, Tri Candra; Nurcholis, Mohammad; Basuki, Basuki; Budiman, Subhan Arif; Yudiantoro, Dwi Fitri
Journal of Degraded and Mining Lands Management Vol. 11 No. 3 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.113.5741

Abstract

The eruption of Mount Semeru at the end of 2021 was responsible for emitting volcanic ash with specific characteristics. These unique mineralogy and chemical properties have both positive and negative effects on soil fertility, as excessive heavy metals adversely affect soil, plants, and the environment. Therefore, this study aimed to determine the distribution of volcanic ash cover from the eruption of Mount Semeru and investigate elemental composition as well as mineral characteristics of volcanic ash and soil covered by volcanic ash. The investigation was carried out in Supiturang village, Pronojiwo District, Lumajang, East Java, Indonesia. Sampling was carried out following the toposequence method, covering agricultural land within approximately ± 15 km from Mount Semeru. Pure volcanic ash was collected at sites not contaminated with soil. At the site location, it was found that the depth of volcanic ash cover ranged from “thin” (<2 cm) to “very thick” (>10 cm). The results of SEM and XRD analyses showed that of the pure volcanic ash contained quartz (50%), rock fragments (15%), plagioclase (12%), hornblende (10%), opaque (8%), and pyroxene (5%) minerals. The results of XRF analysis showed that the dominant elements in volcanic ash and soil were silica (Si), aluminum (Al), calcium (Ca), iron (Fe), and potassium (K). Several non-essential heavy metal elements found were Pb, Sn, and As, while rare minerals discovered were Y, Nb, Eu, and Yb at relatively low concentrations. The SEM analysis showed the structure of volcanic ash dominated by prismatic and blocky.
The Utilization of Serratia marcescens to Increase Phosphate-Availability and Production of Cucumber in Alfisols Abdillah, Muhammad Majid; Setiawati, Tri Candra
Jurnal ILMU DASAR Vol 24 No 1 (2023)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v24i1.29663

Abstract

Serratia marcescens is a bacterium that can dissolve phosphate (P) in soil, increasing phosphate availability and allowing plants to use it to support plant growth. P-available in the soil is generally low, including in Alfisols, due to the formation of bonds with Al, Fe, Ca, Mg, and other cations, which is influenced by soil pH or clay mineral adsorption. Cucumber plants require adequate nutrients, including P nutrients, for optimal growth and production. The study was carried out in a greenhouse using a completely randomized design (CRD) with four treatments and five replications. The treatments were S. marcescens bacterial solution concentrations of P0 (0 ml); P1 (20 ml); and P2 (20 ml); P3 (60 ml) bacterial colony density: 33x109 cfu/ml. According to the findings of the study, S. marcescens inoculation had a significant effect on increasing soil pH by 6,16, P-total concentration of 41,62 mg/100g, P-available concentration of 28,39 ppm, P-tissue concentration shoot and root were 0,30% and 0,78%, respectively, and P-root uptake of 2,27 g/pot. Similarly, S. marcescens inoculation significantly affected the plant height of 138,72 cm, root volume of 43 ml, and root length of 59,92 cm compared to the control treatment.
Pengaruh Pemberian IAA pada Pembibitan Setek Vanili (Vanila planifolia) yang diperkaya Bakteri Pelarut Fosfat dan Bakteri Pelarut Kalium Kusbianto, Dwi Erwin; Emiyati, Sri; Setiawati, Tri Candra; Subroto, Gatot; Rosyady, Mohammad Ghufron
Jurnal Agro Industri Perkebunan Vol. 10 No. 2 (2022)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jaip.v10i2.2550

Abstract

Propagation of vanilla plants is generally using vegetative methods like cuttings. The use of external growth regulators can support the growth of nurseries. Phosphate solubilising bacteria and potassium solubilising bacteria as plant growth promoting rhizobacteria are producing microbial IAA. This study used auxin in synthetic IAA combined with IAA microbes from phosphate solubilising bacteria and potassium solubilising bacteria. This study aimed to determine the effectiveness of each microbe at each dose of synthetic IAA that was applied. The study was conducted from November 2021-April 2022 in the Biological Soil Laboratory and Greenhouse University of Jember. The research uses a randomised block design with three replications with the factorial arrangement. The first factor was organic matter which was enriched with bacterial isolate (B0 = control, B1 = BPF isolate, and B2 = BPK isolate). The second factor was concentration of IAA (A0 = 0 mg.l-1, A1 = 100 mg.l-1, and A2 = 200 mg.l-1). The data were analysed by ANOVA and further tested with Duncan Multiple Range Test (DMRT) with a 95% confidence level. The results showed that treatment of synthetic IAA affects the parameters of length and volume of adventitious roots, which are effective in helping to supply nutrients with the best treatment at a concentration of 200 ppm. Meanwhile, enrichment of media with isolates of phosphate solubilising bacteria and potassium solubilising bacteria gave a better effect than control on the observation of tendril length, number of leaves, number of adventitious roots and taproots, length of adventitious roots and taproots, the volume of adventitious roots and taproot, and fresh weight of vanilla seedlings.
BIOCHAR DAN KOMPOS UNTUK PENINGKATAN SIFAT FISIKA TANAH DAN EFISIENSI PENGGUNAAN AIR Safitri, Indah Nurul; Setiawati, Tri Candra; Bowo, Cahyoadi
TECHNO: JURNAL PENELITIAN Vol 7, No 01 (2018): Techno Jurnal Penelitian
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Biochar merupakan mineral amorf digunakan sebagai bahan pembenah tanah. Biochar dikombinasikan dengan kompos untuk memperbaiki sifat fisika tanah dengan indikator tanaman Jagung Manis. Penelitian dilaksanakan pada bulan Mei-Agustus 2017 di Agrotekhnopark Universitas Jember. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) Faktorial 2x5 dengan tiga ulangan. Faktor pertama adalah kompos terdiri dari kontrol (K0) dan kompos 10 ton/ha (K1) dan faktor kedua adalah biochar terdiri dari kontrol (B0), biochar batang jagung 20 ton/ha (B1) dan 40 ton/ha (B2), biochar jerami padi 20 ton/ha (B3) dan 40 ton/ha (B4). Hasil penelitian menunjukkan pemberian kompos 10 ton/ha dan biochar batang jagung 40 ton/ha mampu menurunkan BV (26,5%), meningkatkan porositas (9,2%), meningkatkan pori air tersedia (61,9%). Pemberian biochar batang jagung 40 ton/ha meningkatkan berat basah (20,6%) dan berat kering tanaman (30,1%). Kombinasi perlakuan kompos (10 ton/ha) dan biochar jerami (40 ton/ha) mampu menurunkan kebutuhan air tanaman sebesar 34,4%. Pemberian biochar mampu mengefisiensi penggunaan air tanaman tertinggi sebesar 0,77 g/mm dibandingkan dengan kontrol sebesar 0,51 g/mm setiap tanaman. Kata kunci: Alfisol, biochar, kompos, efisiensi air, jagung manis
Identifikasi dan Kuantifikasi Metabolit Bakteri Pelarut Fosfat dan Pengaruhnya terhadap Aktivitas Rhizoctonia solani pada Tanaman Kedelai Setiawati, Tri Candra; Mihardja, Paniman Asna
JOURNAL OF TROPICAL SOILS Vol. 13 No. 3: September 2008
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2008.v13i3.233-240

Abstract

Phosphate solubilizing bacteria (PSB) metabolites are organic acids, phosphomonoesterase enzyme (alkaline phosphatase) and antibiotic, which is able to dissolve insoluble phosphate. Phosphate solubilizing bacteria used in this study was expected to suppress Rhizoctonia solani attacks. This experiment was aimed at (1)  identifiying and quantifying  PSB metabolites, and (2) examining their capability as biocontrol agent for Rhizoctonia solani in vitro and hydroponics soybean. This study was conducted in three stages. The first stage of this study was culturing two PSB isolates (Pseudomonas putida 27.4B and Pseudomonas diminuta) in the Pikovskaya medium to analyze their metabolites. The second and third stage of this study was testing the antagonist of two bacteria to suppressed R. solani activity, which was conducted in vitro, and in hydroponics medium soybean as indicator plant. The results showed that P. putida 27.4B and P. diminuta produced organic acids i.e.: citrate, formic, succinic, acetic, propionate, butyrate, and oxalate. The totals of organic acids from each bacterium were 70,3 mg.kg-1 and 61,9 mg.kg-1. Production of alkaline phosphatase enzyme in Pikovskaya medium of P. Putida27.4B was 11,71 μg pNP .mL-1.h-1 and P. diminuta was 24,04  μg pNP.mL-1.h-1. Concentration of this enzyme in soil medium was higher than that in Pikovskaya medium with 26,27 μg pNP.g-1.h-1 and 39,03 μg pNP.g-1.h-1 respectively. This study also showed that total concentration of antibiotics (tetracycline, oxitetracycline and penicillin) produced by the PSB, were 3,2 μg.mL-1 (P. putida 27.4B) and 10,96 μg.m1-1 (P. diminuta), respectively. The results from second stage of this study showed that by using in vitro, the reduced growth of  R. solani was observed 58,35% with P. putida 27.4B and 41,96% with P. diminuta. In addition, inoculations of PSB in hydroponics medium reduced the fungal pathogenesis from 10,71% to 21,42% of pre and post emergence damping-off. Visually, the symptom of pathogen attack appeared within the period of  2 untill 14 days after infection.
Improving Phosphate Efficiency by Phosphate Solubilizing Bacteria and Organic Matter Estimated by Radio Isotop (32P) Technique in Some Soils Mudjiharjati, Arie; Setiawati, Tri Candra; Pandutama, Martinus Harsanto
JOURNAL OF TROPICAL SOILS Vol. 17 No. 3: September 2012
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2012.v17i3.245-252

Abstract

Phosphorous (P) contents in many soils are varies highly along with their ability to provide available P to plant growth. Soils may contain very high in total P, but low in available P due to high P adsorbed by soil matrix and all their adsorbing agents. This research which using natural materials was aimed to increase P availability in some high-P absorption soils. The natural materials utilized for extracting P were organic matter, P extracting bacterial, and rock phosphate. Those materials were interacted to high P absorption soils which were: Oxisol, Inceptisol, and Andisol. The detail objectives of this research were: (1) to study the potential of P-extracting agents (organic matter, and P-extracting bacteria) in releasing P of three high P- absorption soils; (2) to quantify the -age of P coming from the treatments; (3) to calculate the efficiency of P utilization by plant; and (4) to quantify Residual P in soils. The P mobility was analyzed by radioisotope technique using KH232PO4 carrier free solution. The results showed that adding soil organic matter increased the available P by 15.24% in Oxisol, 40.18 in Inceptisol, and by 7.34% in Andisol. Plant sorption toward P from % P used efficiency(%-PUE) up to 60 days was still very low, 0.65 to 9.34%. This was in accordance with the residual P in soils which were still quite high ranging from 94% to 96% in Andisol, 91%97% in Inceptisol, and 96%-98% in Oxisol. The implication of the results of this research, however, is that the application of natural materials in improving soil P availability provides a longtimeresidual effect which could give benefit to the following crops.Keywords: Andisols; Inceptisol; Oxisols; P. diminuta; PUE;  rock phosphate[How to Cite: Mudjiharjati A, TC Setiawati and MH Pandutama. 2012. Improving Phosphate Efficiency by Phosphate Solubilizing Bacteria and Organic Matter Estimated by Radio Isotop (32P) Technique in Some Soils. J Trop Soils, 17 (3) : 245-252. doi: 10.5400/jts.2012.17.3.245][Permalink/DOI: www.dx.doi.org/10.5400/jts.2012.17.3.245]