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All Journal Biotika: Jurnal Ilmiah Biologi Agrovigor: Jurnal Agroekoteknologi Biotropika Indonesian Journal of Pharmaceutical Science and Technology Pro-Life JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education Chemistry Indonesian Journal of Chemical Research AGRIPEAT Al-Ard: Jurnal Teknik Lingkungan Jurnal Biogenerasi Jurnal Laot Ilmu Kelautan Spizaetus: Jurnal Biologi dan Pendidikan Biologi BioEksakta : Jurnal Ilmiah Biologi Unsoed Bioscientist : Jurnal Ilmiah Biologi
Faizah, Hanik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Veterinary Other

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Phytochemical Screening and Antimicrobial Activity of Combination of Piper betle and Moringa oleifera Extracts Arifin, Siti Hakimah Aprilia Garini; Firdhausi, Nirmala Fitria; Hidayati, Irul; Faizah, Hanik
Indonesian Journal of Pharmaceutical Science and Technology Vol 12, No 2 (2025)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v12i2.45288

Abstract

The inappropriate use of antibiotics can lead to pathogenic bacteria developing multiple drug resistance, making infectious disease treatment crucial. Thus, the discovery of novel antibiotics from natural materials, such as plant extracts or their combinations, is critically needed. Extracts of green betel (Piper betle) and Moringa (Moringa oleifera) leaves are known to have antimicrobial activity. This study aimed to determine the chemical content and antimicrobial activity of ethanol extracts of the combination of green betel leaves and Moringa leaves against Staphylococcus aureus, Escherichia coli, and Candida albicans. Green betel and Moringa leaves were macerated in 96% ethanol to extract secondary metabolites. Secondary metabolites were analyzed using phytochemical screening. Antimicrobial activity was tested using disk diffusion using various concentrations: EF1 (25% betel leaves + 75% moringa leaves), EF2 (50% betel leaves + 50% moringa leaves), and EF3 (75% betel leaves + 25% moringa leaves). Phytochemical tests showed that the ethanol extracts of betel leaves contained flavonoids, alkaloids, tannins, and saponins, and no terpenoids, while moringa leaves contain flavonoids, tannins, saponins, and terpenoids, but no alkaloids. S. aureus and E. coli had the highest inhibition zones at EF3 (75% betel leaves + 25% moringa leaves) concentrations, with average inhibition zones of 21.72 mm and 20.98 mm, respectively, and C. albicans at EF2 (50% betel leaves + 50% moringa leaves) concentration, with an average inhibition zone of 26.52 mm, with a very strong inhibition category.
Analisis Kualitas Dan Keamanan Pangan Terasi Melalui Uji TPC Dan Coliform di Laboratorium Kesmavet Sidoarjo Puspitasari, Nurfadilah; Khumairo, Nuris Khumairo; Listianti, Nindira Cahya; Wardiyah, Sri; Faizah, Hanik
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 3 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.3.16156

Abstract

Terasi merupakan salah satu bumbu tradisional Indonesia yang tidak hanya memberikan cita rasa unik pada masakan, tetapi juga mengandung nilai gizi yang tinggi. Kualitas terasi sangat ditentukan oleh mutu bahan baku, metode pengolahan, penanganan produk akhir, serta jenis mikroorganisme yang tumbuh selama proses fermentasi. Penelitian ini bertujuan untuk mengetahui kualitas pangan terasi berdasarkan jumlah total bakteri yang terdapat pada sampel terasi melalui uji TPC, mengetahui tingkat kontaminasi bakteri coliform pada sampel terasi yang diuji di UPTD Laboratorium Keswan Kesmavet Sidoarjo dan mengevaluasi keamanan pangan terasi dengan membandingkan hasil uji TPC dan coliform terhadap standar yang berlaku di bidang keamanan pangan. Penelitian ini menggunakan metode deskriptif kualitatif untuk menganalisis kualitas dan keamanan pangan dengan menentukan nilai TPC dan nilai MPN pada enam sampel terasi yang beredar di pasaran. Hasil penelitian menunjukkan hasil uji Total Plate Count (TPC) pada enam sampel terasi, lima di antaranya memenuhi standar SNI yaitu TR 0 sebesar 2,5 x 10⁴ CFU/gr, TR 1 sebesar 3,7 x 105 CFU/gr, TR 2 sebesar 3,9 x 10⁵ CFU/gram, TR 4 sebesar 7,8 x 10³ CFU/gr dan TR 5 sebesar 3,0 x 10⁵ , sementara 1 sampel terasi melebihi batas yang ditetapkan yaitu TR 3 sebesar 5,7 x 10⁵. Sedangkan hasil uji coliform pada seluruh sampel terasi dari TR 0 hingga TR 5 menunjukkan hasil negatif dengan nilai MPN sebesar <3 MPN/gr. Kata kunci : Coliform, MPN, Terasi, TPC
Uji Resistensi Bakteri Endofit dari Akar Eceng Gondok (Eichhornia crassipes) terhadap Linear Alkylbenzene Sulfonate Arini Nurul Mahmudah; Agustina, Eva; Faizah, Hanik
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 6 No 1 (2025): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v6i1.478

Abstract

Linear Alkylbenzene Sulfonate (LAS) is an anionic surfactant with the potential to cause environmental pollution. One effective bioremediation strategy to mitigate this pollution is the use of endophytic bacteria. This study aims to evaluate the resistance ability of endophytic bacteria isolated from the roots of water hyacinth against LAS. The research was conducted as a laboratory-based experimental study using a Completely Randomized Design with five replications for each isolated endophytic bacterium. Bacterial resistance to LAS was tested in a liquid medium containing 10 ppm LAS and incubated for 14 days. The resistance level was determined based on the bacterial optical density (OD) measured using UV-VIS spectrophotometry. The results identified four endophytic bacterial isolates belonging to the genera Bacillus, Streptomyces, Micrococcus, and Neisseria, all of which exhibited resistance to LAS. The highest resistance level was observed in Streptomyces with an average OD value of 0.728 ± 0.11, while the lowest resistance was found in Bacillus with an average OD value of 0.361 ± 0.04.
The effect of mycorrhizal biofertilizer with the addition of rice washing water and eggshells on the growth of cayenne pepper plant (Capsicum frutescens L.) Sari, Novita Mulya; Rokhim, Saiku; Faizah, Hanik
Agrovigor Vol 17, No 1 (2024)
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v17i1.21768

Abstract

Cayenne pepper is a leading horticultural commodity in Indonesia. The need for cayenne pepper is constantly increasing, but it is not balanced with its production because there are obstacles, especially in areas that are less fertile or have low nutrients. This study aims to find out the effect of applying mycorrhizal biological fertilizer by adding rice washing water and eggshells on the growth of cayenne pepper plants. This study used the Complete Randomized Design method. The data were analyzed using One Way Anova (significant level of 5%) and an alternative test Kruskal-Wallis H. The results showed mycorrhizal biological fertilizer 5 gr + rice washing water 200 ml and eggshells 20 gr optimally affecting the number of leaves, branches, and roots, dry weight of the crown. Mycorrhizal biofertilizer 10 gr + rice washing water 150 ml and eggshell 15 gr have an optimal effect on wet weight of the crown and the roots. Mycorrhizal biofertilizer 15 gr + rice washing water 200 ml and eggshell 20 gr have an optimal effect on root length. Mycorrhizal biofertilizer 2 gr + rice washing water 150 ml and eggshell 15 gr have an optimal effect on dry weight of roots.
Review : Kajian Potensi Bakteri Endofit sebagai Agen Biodegradasi Linear Alkylbenzene Sulfonate (LAS) Mahmudah, Arini Nurul; Faizah, Hanik; Munir, Misbakhul
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10181

Abstract

Linear Alkylbenzene Sulfonate (LAS) is a type of anionic surfactant which is commonly used as a raw material in making commercial detergents or cleaners. Even though LAS can be degraded naturally in the environment, detergent wastewater containing LAS still needs to be treated properly before being discharged into the environment. One way that can be done to reduce the toxic effects of LAS is biodegradation. The use of bacteria, especially endophytic bacteria associated with plants, is known to have potential as a biodegradation agent for LAS. This review aims to examine the potential of endophytic bacteria as LAS biodegradation agents. The method used in this review is a literature review from several international and national journal references. Data were analyzed descriptively and presented in narrative and table form. The results of the research show that several species of endophytic bacteria isolated from various types of plants have the potential to degrade pollutants, such as heavy metals, benzene, phenol, phenanthrene (PHE), diesel, ammonium, phosphate, COD, and hydrocarbons which also shows that endophytic bacteria have the potential to degrades LAS.
Inventarisasi Dan Potensi Gulma Pada Tanaman Jagung (Zea Mays. L) Di Upt Patph Lebo Sidoarjo (Inventory And Potential Weeds In Corn Plants (Zea Mays. L) At Upt Patph Lebo Sidoarjo) Faizah, Hanik; Nurvania Putri, Andini; Bahri, Saiful; Abdillah Ramadhan, Rachmat
Jurnal Biogenerasi Vol. 10 No. 1 (2024): Volume 10 Nomor 1, Agustus 2024 - Februari 2025
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v10i1.4634

Abstract

Weeds are one of the Plant Pest Organisms (OPT) which have both advantages and disadvantages. Weeds can grow around plants very quickly, including in areas where corn is planted. This research aims 1. To describe the weed diversity index found in corn plants (Zea mays L.) at UPT PATPH Lebo Sidoarjo. 2. To determine the morphological structure of weeds found in corn plants (Zea mays L.) at UPT PATPH Lebo Sidoarjo. 3. To analyze various potential weeds found in corn plants (Zea mays L.) at UPT PATPH Lebo Sidoarjo. The method used is a survey using random sampling techniques with a plot system. The results showed that at stations I and II the most dominant plant was Portulaca oleracea (Purslane). The diversity index in the corn fields of UPT PATPH Lebo at stations I and II is 1.96, which means moderate diversity. which means H' is classified as medium (1<H'<H’3). The identified potential of weeds is as food, animal feed, medicine and ornamental plants.
Kajian Kelayakan Sumber Air Tanah UIN Sunan Ampel Surabaya dalam Rangka Menuju Eco Campus Fadhillah, Ninik; Ma’arif, Muh.; Faizah, Hanik; Chilmi, Latifatoel
Al-Ard: Jurnal Teknik Lingkungan Vol. 5 No. 1 (2019): September
Publisher : Department of Environmental engineering, Faculty of Science and Technology, Islamic State University Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/alard.v5i1.475

Abstract

UIN Sunan Ampel requirements of water are fulfilled from groundwater sources. The groundwater sources of UIN Sunan Ampel are from wells where the water is taken with a water pump. The main problem related to the use of groundwater as a fulfillment of water needs is the decline of its quality. The purpose of this study is to analyze the quality of groundwater at UIN Sunan Ampel, to know the quality of groundwater in UIN Sunan Ampel reviewed by Minister of Health Regulation No. RI. 32 of 2017, Formulating the right steps in improving the quality of ground water at Sunan Ampel UIN towards ECO CAMPUS. This research is a descriptive study, in this study the quality of groundwater sources in Sunan Ampel Surabaya UIN will be described base on 3 parameters of water quality (physics, chemistry and biology). The results indicate differences in groundwater quality at UIN Sunan Ampel in terms of physical, chemical and biological parameters. The quality of groundwater sources in UIN Sunan Ampel Surabaya 95% fulfills the standard of environmental health standards and water health requirements for government sanitation hygiene requirements based on physics parameters, but it do not fulfilled the quality standards based on chemical parameters (in the form of pH) and also biology parameters. Improving the quality of ground water at UIN Sunan Ampel Surabaya is needed by improving the management system of sewers and latrines as well as standard construction of wells.
The Effect of IBA and NAA on Root Induction of Tithonia diversifolia (Hemsl.) Ramadhani, Dwi Nova; Hidayati, Irul; Faizah, Hanik
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.06

Abstract

Tithonia diversifolia (Hemsl.) is a plant that has a lot of potential for use in several sectors, so that large-scale and short-time propagation efforts are needed. Tissue culture is an appropriate technique for cultivating Tithonia diversifolia plants. The success of root induction in tissue culture is largely determined by the auxin growth regulator. This study aimed to determine the effect of IBA and NAA on root induction of T. diversifolia plants. The method was an experimental study with a Completely Randomised Design (CRD). Tithonia diversifolia plant node explants were planted into Murashige and Skoog (MS) media supplemented with growth regulators IBA (1 and 2 mg/L), NAA (1 and 2 mg/L), and a combination of IBA and NAA (1 mg/L + 1 mg/L; 1 mg/L + 2 mg/L; 2 mg/L + 1 mg/L; and 2 mg/L + 2 mg/L) and control group, consisting of explants cultured on a hormone-free medium which were then incubated for 4 weeks. The observation data were analysed statistically using the non-parametric Kruskal-Wallis test, followed by the Mann-Whitney test. The results showed that all parameters significantly affected auxins IBA and NAA in the root induction of T. diversifolia plants. The NAA 2 ppm treatment was the most effective in terms of root emergence time (3.67 DAP). Additionally, the combination of IBA and NAA was the optimal treatment in terms of root growth percentage (1.00%). The IBA 2 ppm + NAA 2 ppm treatment yielded the best results in terms of root number (10.17 strands). The NAA 1 ppm treatment yielded the best results in terms of root length (11.3 cm), root fresh weight (0.0967 g), and root dry weight (0.0144 g).
UJI KUALITAS MIKROBIOLOGI PADA DAGING AYAM BROILER YANG DIJUAL DI PASAR TRADISIONAL KECAMATAN BANGIL Muhtar, Nurul Maulidiya; Faizah, Hanik; Firdhausi, Nirmala Fitria
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 2 (2025): BIOTIKA DESEMBER 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i2.64903

Abstract

Keamanan pangan merupakan aspek penting yang menentukan kualitas kesehatan masyarakat, terutama pada produk hewani seperti daging ayam. Penanganan dan penyimpanan yang tidak higienis dapat menyebabkan terjadinya kontaminasi mikroba yang berpotensi menurunkan kualitas serta membahayakan konsumen. Penelitian ini bertujuan untuk mengetahui kualitas mikrobiologi daging ayam broiler yang dijual di pasar tradisional Kecamatan Bangil, Kabupaten Pasuruan, dengan fokus pada parameter Total Plate Count (TPC), keberadaan Escherichia coli, dan Salmonella sp. Metode yang digunakan adalah deskriptif kualitatif dengan teknik sampling jenuh. Sebanyak 10 sampel daging diuji menggunakan metode TPC, media EMBA untuk E. coli, dan media SSA untuk Salmonella sp. Hasil uji menunjukkan bahwa satu sampel (10%) melebihi ambang batas TPC berdasarkan SNI 7388:2009 yaitu 1×10⁶ CFU/g, sedangkan sembilan lainnya masih dalam batas aman. Pengujian E. coli menunjukkan bahwa 3 dari 10 sampel (30%) positif terkontaminasi, dan uji Salmonella sp. juga menunjukkan 3 sampel (30%) positif. Temuan ini menunjukkan bahwa meskipun sebagian besar sampel memenuhi standar mutu mikrobiologis, masih terdapat risiko kontaminasi patogen yang dapat membahayakan kesehatan konsumen jika daging tidak dimasak dengan benar. Oleh karena itu, diperlukan pengawasan sanitasi dan edukasi terkait penanganan daging ayam di pasar tradisional
Co-Authors Abdillah Ramadhan, Rachmat Arifin, Siti Hakimah Aprilia Garini Arini Nurul Mahmudah Azzahra, Fadila Kamilia Burhana, Nanda Azizah Chilmi, Latifatoel Dian Novitasari Eko Teguh Pribadi Esti Tyastirin Eva Agustina, Eva Handayani, Ade Tiyan Irul Hidayati Khumairo, Nuris Khumairo Linggar Dwi Kinasih Listianti, Nindira Cahya Mahmudah, Arini Nurul Ma’arif, Muh. MISBAKHUL MUNIR Misbakhul Munir Moch. Irfan Hadi Muhtar, Nurul Maulidiya Ninik Fadhillah Nirmala Fitria Firdhausi Nurvania Putri, Andini Puspitasari, Nurfadilah Putri, Vivin Diana Ramadhani, Dwi Nova Rosyada, Fanny Fajrin Aulia Saiful Bahri Saiku Rokhim Sari, Novita Mulya Siti Khodijah Surfiyanti, Oktarina Veithzal Rivai Zainal Wardiyah, Sri