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All Journal Jurnal Kesehatan Biota JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Universa Medicina Journal of Health Science and Prevention AGRIPEAT AL-HAYAT: Journal of Biology and Applied Biology Biota Scripta Biologica Al-Ard: Jurnal Teknik Lingkungan Jurnal Riset Biologi dan Aplikasinya Indian Journal of Forensic Medicine & Toxicology Jurnal Biosense Nusantara Journal of Community Engagement Global Health Science (GHS) JURNAL BIOLOGI PAPUA Engagement: Jurnal Pengabdian Kepada Masyarakat Journal of Marine Resources and Coastal Management Folia Medica Indonesiana BIOTROPIC
Moch. Irfan Hadi
Universitas Islam Negeri Sunan Ampel Surabaya
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Analysis of the Moringa oleifera Seed Oil Extract on Insulin Level in Alloxan-Induced Diabetic Rat (Rattus norvegicus) Estri Kusumawati1 , Sri Hidayati L.1 , Nova Lusiana1 , Risa Purnamasari1 , Moch. Irfan Hadi1
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10695

Abstract

Moringa oleifera is a plant that can be used and grown intensively in several Southeast Asian countries. This study aims to determine the effect of Moringa oleifera seed oil extract on the levels of the insulin and blood glucose levels of Rattus norvegicus induced by alloxan. This study used 30 female Wistar white rats induced by alloxan. Rats were divided into 6 groups. Groups 3 to 6 were injected 100, 200, 300, 400 mg/kg BW extract of Moringa oleifera seed oil, respectively. Group 1 neither had alloxan induction nor treatment while group 2 had been induced alloxan but no treatment. Data were analyzed using the Kruskal-Wallis test with a significance level of 0.05. The results showed that there was no effect of Moringa oleifera seed oil extract on insulin level (p value = 0.161). Conversely, there was an effect on blood glucose levels in alloxan-induced Rattus norvegicus (p value = 0.036).
Detection of Emerging Infectious Disease in Cynopterus brachyotis and Rhinolopus boorneensis as Reservoirs of Zoonotic Diseases in Indonesia Moch. Irfan Hadi1 , Munawir1 , Muhammad Yusuf Alamudi2 , Dedi Suprayogi1 , Mirna Widiyanti3
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10725

Abstract

Bats are mammal species that have the highest diversity. During the last two decades, various types of infections caused by microorganisms have again become epidemic in many countries, this phenomenon is known as a new infectious disease that arises and re-emerges or Re-Emerging Infectious Diseases (EID/ REID). The purpose of this study was to identify EID in bats in the karst area of South Malang. The research method of bat sampling is conducted in the karst area of South Malang, bats are identified by species, taken by blood, prepared and carried out by NGS (next generation sequencing). From the results of the research conducted, obtained 2 types of bats namely fruit-eating bats (Chiroptera brachjatis) and insect bats (Rhinolopus boorneensis). From the results of the NGS, we get specific viruses and bacteria in bats. The conclusion of this research is that bat in South Malang Karst has potential as a carrier of EID and zoonotic disease.
Body mass index increases CD4+ count in HIV/AIDS patients on first-line therapy Widiyanti, Mirna; Hadi, Moch Irfan; Kumalasari, Mei Lina Fitri; Natalia, Evi Iriani; Purba, Dedi Ananta; Adiningsih, Setyo
Universa Medicina Vol. 39 No. 2 (2020)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2020.v39.121-127

Abstract

BackgroundThe body mass index (BMI) may contribute somewhat to drug metabolism, thus affecting the efficacy of antiretroviral therapy (ART). CD4+ counts   in people infected with HIV are essential in determining the stage of the disease, initiation of antiretroviral therapy, opportunistic infections and evaluating treatment outcomes. The aim of this study was to determine the association of BMI and clinical stage with CD4+ counts in HIV patients seeking treatment using first-line antiretroviral therapy (ART).MethodsAn analytic study with a cross-sectional approach was conducted involving 251 HIV/AIDS patients who had received first-line antiretrovirals over six months. BMI, clinical staging according to WHO and CD4 + were collected. Multiple linear regression was used to evaluate the relationship between BMI, clinical stage and CD4+.ResultsAmong the enrolled patients, the median age was 36 years, 135 (55%) of the patients was female, 102 (40.6%) was overweight/obese, 161 (64.1%) was in stage 3 of the disease, and the median CD4+ count was 389 cells/mm3. Multiple linear regression test showed two variables with a significant effect on CD4+ count, namely BMI (B=69.247; 95 % CI : 42.886-95.608) and clinical stage (B=61.590; 28.910-94.270). BMI was the most influencing factor for CD4+ count (β=0.307) compared to clinical stage (β=0.216). ConclusionsBody mass index was the most influencing factor for CD4 + counts of HIV/AIDS patients. Regular ART can increase CD4+ counts and maintain the health of HIV/AIDS patients.
Immunoinformatics Analysis of SARS-CoV-2 Isolated from Karst of Bats in Malang, Indonesia Moch Irfan Hadi; Aditya Sukma Prawira; Khuliyah Candraning Diyanah; Dedy Suprayogi; Soedjajadi Keman; Yudied Agung Mirasa; M Yusuf Alamudi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14805

Abstract

In December 2019, cases of mysterious pneumonia were first reported in Wuhan, Initially, the diseasewas temporarily named as 2019 novel coronavirus (2019-nCoV), then WHO announced a new name onFebruary 11, 2020, namely Coronavirus Disease (COVID-19) which is caused by a virus Severe AcuteRespiratory Syndrome Coronavirus-2 (SARS-CoV-2). This virus can be transmitted from person toperson and has spread widely in China and more than 190 other countries and territories. Meanwhile inIndonesia, up to September 2020, 203 thousand cases of COVID-19 were found and 8336 deaths. Batsact as important disease reservoirs for various etiologic agents of disease that can be transmitted betweenspecies, infecting humans and mammals, both domestic and wild. The purpose of this study was to analyzeSARS-CoV-2 immunoinformatics in bats in Karst Malang Indonesia as the basis for making SARS-CoV-2vaccines. This research method is SARS-CoV-2 amino acid bats in Karst Malang, Indonesia analyzed byimmunoinformatics. From the research results, it was found that SARS-CoV-2 protein from Malang Karstbats were antigen, non-allergen and non-toxin, so that they could be used as vaccine candidates, diagnostickits and immunotherapy.
Leptospirosis Transmission in Ponorogo District of East Java, Indonesia Aditya Sukma Pawitra; Khuliyah Candraning Diyanah; Corie Indria Prasasti; Moch Irfan Hadi; M. Yusuf Alamudi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15596

Abstract

This study aims to identify Leptospira bacterial infection in livestock owners and their livestock, as well as to examine potential risk factors correlated with the incidence. 50 participants were selected and their blood samples were collected. 50 urine samples were collected from livestock owned by the participants. Polymerase Chain Reaction (PCR) was used to identify the existence of the Leptospira bacteria. The questionnaire instrument was used to obtain information about individual characteristics and hygiene. The Chi-Squared test was adopted to examine the correlation between outcome and explanatory variables. The confirmation PCR test detected the bacterial DNA in 2 out of 50 blood samples examined (4%) and 3 out of 50 urine samples examined (6%). Human leptospirosis incidence is significantly correlated with occupation type (p=0.035), personal protective equipment (PPE) use (p=0.044), water puddle contact (p=0.044), cage sanitation (p=0.044) and Leptospira bacteria presence in livestock urine (p=0.007). Insignificant correlation was showed in owners’ age variable. The presence of Leptospira bacteria both in livestock and the owners indicates the real threat of animal to human transmission. Further study with larger sample size and wider range variables and meticulous examination technique is required to comprehend the investigation.
Leptospirosis Transmission in Ponorogo District of East Java, Indonesia Corie Indria Prasasti; Aditya Sukma Pawitra; Khuliyah Candraning Diyanah; Moch Irfan Hadi; M. Yusuf Alamudi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15737

Abstract

This study aims to identify Leptospira bacterial infection in livestock owners and their livestock, as well as to examine potential risk factors correlated with the incidence. 50 participants were selected and their blood samples were collected. 50 urine samples were collected from livestock owned by the participants. Polymerase Chain Reaction (PCR) was used to identify the existence of the Leptospira bacteria. The questionnaire instrument was used to obtain information about individual characteristics and hygiene. The Chi-Squared test was adopted to examine the correlation between outcome and explanatory variables. The confirmation PCR test detected the bacterial DNA in 2 out of 50 blood samples examined (4%) and 3 out of 50 urine samples examined (6%). Human leptospirosis incidence is significantly correlated with occupation type (p=0.035), personal protective equipment (PPE) use (p=0.044), water puddle contact (p=0.044), cage sanitation (p=0.044) and Leptospira bacteria presence in livestock urine (p=0.007). Insignificant correlation was showed in owners’ age variable. The presence of Leptospira bacteria both in livestock and the owners indicates the real threat of animal to human transmission. Further study with larger sample size and wider range variables and meticulous examination technique is required to comprehend the investigation.
Optimalisasi Pengetahuan Tentang Menstruasi Pada Siswa Putri Kelas 5 dan 6 SDN Janti 1 Waru Sidoarjo Dengan Pendekatan PAR Mei lina Fitri Kumalasari; Nova Lusiana; Estri Kusumawati; Dedy Suprayogi; Moch. Irfan Hadi
Nusantara Journal of Community Engagement Vol. 1 No. 1 (2020): MARET
Publisher : Lembaga Penelitian dan Pengabdian (LP2M) Sekolah Tinggi Agama Islam Cendekia Insani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.2020/njce.v1i1.3664

Abstract

Anak perempuan yang mengalami menarche (menstruasi pertama) pada usia yang muda merasa belum siap untuk menghadapi menstruasi pertamanya sehingga dapat menimbulkan dampak takut, bingung, minder, merasa tidak normal dan panik. Pengabdian masyarakat ini bertujuan untuk optimalisasi pengetahuan tentang menstruasi pada siswa putri kelas 5 dan 6 di SDN Janti 1. Metode kegiatan yang dilakukan adalah dengan pendekatan PAR. Didapatkan hasil bahwa dari 89 siswa putri, terdapat 40 siswa putri (44,94%) yang sudah mendapatkan menstruasi. Siswa putri tersebut mengalami menarche pada usia 10 sampai dengan 12 tahun. Terdapat 22 siswa putri (55%) yang mengalami dismenorea dan belum mengetahui apa yang harus dilakukan apabila mengalami keluhan tersebut. Materi diberikan dengan media powerpoint dan video. Siswa putri kelas 5 dan 6 SDN Janti 1 aktif dalam kegiatan dengan memberikan pertanyaan-pertanyaan dan saling berbagi pengalaman.
HUBUNGAN TINGKAT STRES PSIKOLOGIS DENGAN SIKLUS MENSTRUASI PADA MAHASISWA Mei Lina Fitri Kumalasari; Moch Irfan Hadi; Misbakhul Munir
Jurnal Kesehatan Vol 12 No 2 (2019): JURNAL KESEHATAN
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/kesehatan.v12i2.10842

Abstract

The menstrual cycle is the distance between the first day of menstruation and the first period of the next menstruation. One of the faktors that influence the menstrual cycle is stres. Stres can stimulate the release of the hormone cortisol, which is a product of glucocorticoid adrenal cortex. This hormone affects the amount of progesterone in the body. This hormonal imbalance will cause changes in the menstrual cycle. The purpose of this study was to analyze the relationship between stres levels and the menstrual cycle of UIN Sunan Ampel Surabaya students. This research was an observational analytic study with a sampling design using simple random sampling with a total of 30 respondents. Data collection techniques use the DASS 42 questionnaire to measure stres levels and the menstrual cycle pattern questionnaire to measure the menstrual cycle. Data analysis using Fisher's exacs test and the results obtained p = 0.031, so it means that there is a relationship between the level of stres with the menstrual cycl.ABSTRAKSiklus menstruasi adalah jarak antara hari pertama menstruasi dengan hari pertama pada menstruasi berikutnya. Hal ini dapat dipengaruhi oleh stress yang merangsang pengeluaran hormone kortisol yang merupakan produk dari glukokortiroid korteks adrenal yang disintesis di zona fasikulata. Hormon ini mempengaruhi jumlah hormone progesterone di dalam tubuh. Ketidakseimbangan hormon ini akan menyebabkan perubahan siklus menstruasi. Tujuan dari penelitian ini adalah untuk mengetahui hubungan antara tingkat stres dengan siklus menstruasi mahasiswai UIN Sunan Ampel Surabaya. Penelitian ini merupakan penelitian observasional analitik dengan desain pengambilan sampel menggunakan simple random sampling dengan jumlah 30 responden. Tehnik pengumpulan data menggunakan kuesioner DASS 42 untuk mengukur tingkat stres dan kuesioner pola siklus menstruasi untuk mengukur siklus menstruasi. Analisis data menggunakan Fisher’s exacs test dan didapatkan hasil nilai p=0.031. Hal ini berarti menunjukkan bahwa ada hubungan hubungan antara tingkat stres dengan siklus menstruasi. Penelitian selanjutnya sebaiknya menambahkan variabel lain yang mempengarusi siklus menstruasi dan menamba jumlah sampel agar diperoleh data yang lebih baik.
Optimization of PCR Protocol for the Identification of Yeasts Isolated from Apis mellifera Honeycomb based on the ITS rDNA Ayudya Fitri Arifa; Nirmala Fitria Firdhausi; Irul Hidayati; Yuanita Rachmawati; Moch. Irfan Hadi
Biota Vol 8 No 2 (2022): Jurnal Biota 2022
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/Biota.v8i2.10181

Abstract

Yeast is a microorganism that can be found in honeycomb. Yeast identification is a process to find and identify new species. One of which is molecular identification of yeast with rDNA sequences in the ITS region. Before carrying out molecular identification, it is necessary to optimize yeast DNA amplification methods to obtain good DNA sequences that ease the yeast identification process. The purpose of this study was to discover the optimum PCR (Polymerase Chain Reaction)protocols for the identification of yeasts isolated from Apis mellifera honeycomb based on the ITS rDNA. This study used 3 PCR (Polymerase Chain Reaction) protocols, i.e., from Kanti et al. (2018), Ediningsari (2008), and Maulana (2011). This results study shows that the optimum PCR protocol was from Maulana (2011), which produced clear and whole DNA fragment luminescences.
Management of Sars-Cov-2 Medical Waste Against a Covid19 Pandemic in Indonesia: A Literature Review Moch. Irfan Hadi; Mirna Widiyanti; Mei Lina Fitri Kumalasari; Muhammad Yusuf Alamudi; Dedy Suprayogi
JURNAL KESEHATAN LINGKUNGAN Vol. 12 No. 4 (2020): JURNAL KESEHATAN LINGKUNGAN
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jkl.v12i4.2020.244-253

Abstract

Introduction: SARS-CoV-2 RNA transmission in wastewater has a risk of fecal transmission of SARS-CoV-2 to humans and the environment. Despite the increasing number of cases in almost all areas in Indonesia, the study to review and evaluate waste management resulted from medical facilities is still limited and less explored. This literature review aims to analyze the applicability of WHO guidelines on wastewater management to be implemented in Indonesia. Scientific papers were collected from several electronic databases such as PubMed, PLoS, Researchgate, WHO, Elsevier, Science, SARS-CoV-2, Covid19, and wastewater. The articles were selected based on the inclusion criteria that use SARS-CoV-2, Covid19, and wastewater as the main variables observed. The articles published before 2013 were excluded from this review. Discussion: Wastewater monitoring of SARS-CoV-2 within medical and health facilities is implemented to identify the virus's presence as the causative agent of Covid19 disease, which contaminated the environment and society. The results of the analysis in wastewater can be used to determine the infection control in health facilities while also beneficial in formulating the monitoring of environmental impacts caused by the waste. These efforts would support policies or interventions based on public health by applying social distances, locking, quarantine areas that aim to break the Covid19transmission. Conclusion: Briefly, by considering the urgency of Covid19 pandemic containment and control measures of transmission in health facilities, population and environment. It is a waste management guideline that is highly required to be implemented based on public health aspects.
Co-Authors Abdul Azis Abdul Muhid Adiningsih, Setyo Aditya Sukma Pawitra Aditya Sukma Prawira Agung, Tri Sunan AGUSTINA, EVA Albarkah, M. Rafly ANDYARINI, ESTI NOVI Annisa Inda Sanabila Asri Sawiji Atiqoh Zummah Ayudya Fitri Arifa Choiriyah, S.Si, Chumaidatul Corie Indria Prasasti Dedi Ananta Purba Dedy Suprayogi Dian Tugu Warsito Taufiq Eko Teguh Pribadi Esti Tyastirin Estri Kusumawati Eva Agustina Eva Agustina, Eva Evi Iriani Natalia Faizah, Hanik Fatih, M. Fachrul Funsu Andiarna Hanik Faizah Ika Mustika Irul Hidayati Izza Mahendra Kartika Dewi Kartika Dewi Khuliyah Candraning Diyanah Linggar Dwi Kinasih M Yusuf Alamudi M. Chusnan Ma'arif M. Yusuf Alamudi Mauludiyah Ma’arif, M. Chusnan Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei Lina Fitri Kumalasari Mei lina Fitri Kumalasari Mei Lina Fitri Kumalasari Miftakhus Sa'adah Mirna Widiyanti Mirna Widiyanti Mirna Widiyanti Mirna Widiyanti Misbakhul Munir MISBAKHUL MUNIR Misbakhul Munir Muhamad Ratodi Muhammad F. Musyaffa Muhammad Falikhul Musyaffa' Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Muhammad Yusuf Alamudi Nadlir Nadlir Nadlir Nadlir, Nadlir Nanang Supriatna Natalia, Evi Iriani Nirmala Fitria Firdhausi Nova Lusiana Nugraha, Dimas Putra Wahyu Nugraha, Dimas Rizal Wahyu Nur Maulida Safitri Nusa, Raras Widya Perdanawati, Rizqi Abdi Puji Maryono Purba, Dedi Ananta Putra, Riswanda Rangga Risa Purnamasari Rizqiyah, Ziadatur Robayanto Robayanto Sanabila, Annisa Inda Setyo Adiningsih Setyo Adiningsih Sigit Ari Saputro Soedjajadi Keman Sri Hidayati Sri Hidayati Suhardi, Haidar Rafli Putra Syahrul Afifa Ratna Sari Tatag Bagus Prakarsa Teguh Taruna Utama Widia Purnamasari Wiga Alif Violando Yuanita Rachmawati Yudied Agung Mirasa