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All Journal Agrovigor: Jurnal Agroekoteknologi Indonesian Journal of Tropical and Infectious Disease Pro-Life BIOTROPIC The Journal of Tropical Biology JRST (Jurnal Riset Sains dan Teknologi) Biosel: Biologi Science and Education Indonesia Journal of Halal Journal of Health Science and Prevention Cakrawala: Jurnal Litbang Kebijakan Indonesian Journal of Halal Research JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Journal of halal product and research (JHPR) BIO-EDU: Jurnal Pendidikan Biologi Jurnal Ilmiah Membangun Desa dan Pertanian

Saiku Rokhim

Universitas Islam Negeri Sunan Ampel Surabaya

Author-ID : 856126

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Keanekaragaman dan Kemerataan Jenis Collembola Gua di Kawasan Karst Malang Selatan widya pertiwi; Saiful Bahri; Saiku Rokhim; Nirmala Fitria Firdhausi
Biotropic : The Journal of Tropical Biology Vol. 4 No. 2 (2020): Biotropic, Volume 4 Nomor 2, 2020
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2020.4.2.134-139

Karst adalah bentang alam yang unik yang terbentuk akibat dari pelarutan batuan karbonat atau batuan gamping oleh air hujan. Di dalam kawasan karst terbentuk beberapa gua yang menjadi ci khas dari kawasan karst yang terbentuk. Penelitian ini bertujuan untuk mengetahui keanekaragaman Collembola gua yang berada di Kawasan Karst Malang Selatan. Sampel yang diambil adalah berasal dari tanah yang berada di lantai gua dengan menggunakan teknik Purposiv Sampling. Penelitian dilakukan pada empat gua yaitu Gua Harta, Gua Krompyang, Gua Prapatan JLS, dan Gua Lowo. Setiap pengambilan sampel dilakukan tiga kali pengulangan pada setiap zona, yaitu zona terang, zona remang, dan zona gelap. Hasil analisis data menghasilkan nilai Indeks Keanekaragaman H’=0,46121 dan Indeks Kemerataan E=0,28656.

End Point Polymerase Chain Reaction for Porcine Detection on Food Product of UIN Sunan Ampel Surabaya Canteen Saiku Rokhim; Inggrit Tyautari; M. Aliffiyan Firmansyah; Yuanita Rachmawati
Indonesian Journal of Halal Research Vol 3, No 1 (2021): February
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v3i1.11149

Halal food means food that permitted under Islamic law and fulfills about requirements. The absence of information about halal food contained in UIN Sunan Ampel Surabaya (UINSA) campus area causes related research to be carried out. This study aims to determine the porcine DNA contamination on food around UINSA area using molecular technology. Twenty two samples used were foods that contain meat and may contain pork obtained from canteens around UINSA area, analyzed using Polymerase Chain Reaction (PCR) method. The analysis was started with DNA isolation of 22 food samples, electrophoresis, PCR, then visualization gel electrophoresis. Primer gene coding for cytochrome b (cyt b) which produces 149 bp of DNA fragments. The results showed that no porcine contamination in 22 food samples, while the positive control showed a band of 149 bp. End point PCR method potentially to detect porcine DNA contaminants in food products around UINSA. Therefore the food is halal and safe for consumption.

Sus sp. DNA Encoding cyt b Gene Detection Test on Meat Grinding Samples Using Conventional PCR Miftahul Lathif Adzakiyyi; Tri Susilowati; Saiku Rokhim; Yuanita Rachmawati
Indonesian Journal of Halal Research (IJHAR) Vol 2, No 2 (2020): August
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ijhar.v2i2.8489

Micro-entrepreneurs with basic ingredients of processed meat such as meatball who do not have a meat grinder, generally using meat grinder at the public market. The problem that occurs is that there is no clear regulation from the Government regarding the guarantee of the halal meat grinding in the Regional Company. This needs to be enhanced as a study, considering that the grinding material does not only come from Halal substances. The purpose of this study was to test pig DNA in meat grinding samples at PD Pasar Surya Surabaya City by using the conventional Polymerase Chain Reaction (PCR) method. DNA was isolated from 11 PD Pasar Surya meat grinding samples, then spectrophotometry was performed. Spectrophotometry results showed that all samples have high DNA concentrations. The primer used is the cyt b pig gene encoder. Predenaturation is performed at a temperature of 95°C-5 minutes, denaturation of 95°C-45 seconds, annealing 60°C-30 seconds, extension 72°C-40 seconds, and post extension 72°C-5 minutes. The results of PCR analysis were determined by the emergence of DNA bands of ± 149 bp as markers of pig DNA. The results showed negative on sample or no pig contamination in 11 samples tested. While the pig sample as positive control showed a band of ± 149 bp. These results prove that at 11 points of the location of meat grinding there is no contamination of pig DNA.

DETEKSI KONTAMINAN FRAGMEN DNA PENGKODE cyt b BABI PADA SAMPEL SOFTGELLCANDY TAK BERLABEL HALAL Yuanita Rachmawati; Saiku Rokhim; Misbakhul Munir; Eva Agustina
Indonesia Journal of Halal Vol 1 (1) 2018
Publisher : Pusat Kajian Halal Undip

Abstrak Softgellcandy adalah permen bertekstur lunak yang diproses dengan penambahan komponen hidrokoloid seperti agar, gum, pektin, pati, kaegenan, gelatin dan lain-lain yang digunakan untuk modifikasi tekstur sehingga menghasilkan produk yang kenyal. Sayangnya distribusi Softgellcandy di pasaran seringkali terlepas dari pengawasan lembaga berwenang. Banyak ditemukan bermacam merk Softgellcandy yang tidak berBPOM maupun tidak berlabel Halal. Gelatin menjadi titik kritis kehalalan Softgellcandy. Penelitian ini menguji 15 sampel Softgellcandy tak berlabel halal yang dijual bebas di Surabaya dengan primer pengkode fragmen DNA cytochrome b Babi. Metode yang digunakan adalah konvensional PCR pada suhu 98oC-2 menit; 95oC-30 detik; 61oC-30 detik; 72oC-40 detik; dan 72oC-3 menit, selama 30 siklus. Visualisasi hasil PCR menggunakan elektroforesis 2% gel agarosa menunjukkan dari 15 sampel, 8 sampel terindikasi kontaminan DNA babi ditandai dengan pita DNA sebesar ±149bp. Pemerintah perlu melakukan monitoring lebih ketat terkait peredaran produk makanan tak berlabel halal yang dijual bebas di pasaran, mengingat Halal menjadi issue yang sangat sensitif di negara dengan mayoritas penduduk muslim terbesar di dunia ini. Karena halal adalah suatu keharusan. Keywords: Softgellcandy, cyt b Babi, PCR

Risk Assessment to Dust Exposure in Room Maintenance: Analytical Study on Room Maintenance at PT. X (Persero) Building in Surabaya Saiku Rokhim
Journal of Health Science and Prevention Vol. 1 No. 1 (2017): JHSP Vol 1 No 1 - 2017
Publisher : State Islamic University of Sunan Ampel

As one of the particulate chemicals, dust could occur in most of the production process and can create interference for workers health and safety. As one of the air pollution sources, dust could became a potential hazard which exist in room maintenances. Protection to workers is a must in order to reduce the risk of respiratory tract syndrome that often could be found in this cases. The aim of this study is to conduct a risk assessment to dust exposure in room maintenance, which held by contractors in PT. X (Persero) building in Surabaya. This is an cross sectional study with obsevation approach. The object of this research is the repairing works. The results indicate that the activities which could produce dust, such as: walls sanding using sandpaper, the tiles dismantle, sawmilling, the wood fiber refining, grinding, mixing and stirring cast materials, and room cleaning. Dust produced from a variety of works including sanddust, cement, lime, wood and dust mixed with paint. The results show that three types of works considere as high-risk activity (value > 12-25), 3 types of work consider as midle risk activities (value > 5-12), and one activity considered as a low-risk work (grades 1-5). The dusk factors controlling should be held regularly, in order to minimize the risk leveln againts the workers.

Kontaminasi Bakteri Escherichia coli pada Sampel Daging Saiful Bahri; Saiku Rokhim; Yosi Setia Prasiska
Journal of Health Science and Prevention Vol. 3 No. 1 (2019): JHSP Vol 3 No 1 - 2019
Publisher : State Islamic University of Sunan Ampel

Pangan yang berasal dari ternak sangat kita butuhkan karena mengandung protein penting dan mudah dicerna, daging merupakan salah satu pangan yang berasal dari ternak dan sangat bermanfaat bagi tubuh karena mengandung gizi yang lengkap sehingga perlu untuk dilakukan pencegahan kontaminasi terutama oleh bakteri Eschericia coli. Tujuan dari penelitian ini adalah untuk mengetahui kontaminasi bakteri Escherichia coli pada beberapa sampel daging. Penelitian ini termasuk dalam penelitian eksperimental untuk mengidentifikasi bakteri Escherichia coli pada beberapa daging segar menggunakan metode pengenceran dan lama waktu inkubasi yang berbeda dari setiap sampel. Berdasarkan penelitian yang telah dilakukan diperoleh jumlah kontaminasi bakteri Escherichia coli tertinggi pada sampel daging ayam daripada daging sapi dan daging kambing. Hasil tertinggi kontaminasi bakteri Escherichia coli daging ayam yang melebihi BMCM adalah sebesar 1108 : 1 x 109 dengan kode DA 049-DA 058. Kesimpulan berdasarkan hasil yang diperoleh bahwa 18 sampel daging ayam sebanyak (50%) sampel terkontaminasi Escherichia coli, 13 sampel daging sapi sebanyak (38%) terkontaminasi Escherichia coli. Sedangkan 1 daging kambing (0%) tidak terkontaminasi bakteri Escherichia coli.

Patogenitas Nematoda Entomopatogen Heterorhabditis spp. Terhadap Larva Spodoptera litura Hawwa' Cahya Maulida; Saiku Rokhim; Erna Zahro’in
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 6, No 2 (2021)
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v6i2.809

Spodoptera litura is a pest in vegetable plants that attacks the leaves and stems. Damage arising from the attack of S. litura can decrease the productivity of plants. Synthetic pesticides are often used in controlling the population of S. litura, but synthetic pesticides have a high negative impact. Potential entomopathogenic nematode Heterorhabditis spp. believed to be effective for controlling pest populations. This study aims to find out the pathogenic value of entomopathogenic nematode Heterorhabditis spp. to the larvae of S. litura. Based on the results obtained Heterorhabditis spp. has a positive influence in causing mortality in the larvae of S. litura. Heterorhabditis spp. mortality of up to 42%. Symptoms indicated by larvae of S. litura affected by Heterorhabditis spp. among them the behavior becomes passive, the body becomes flaccid, the cuticle turns red and the tissues inside the body are destroyed. Obtained pathogenicity value Lc 50 Heterorhabditis spp. 7,690 IJ/ml, as for the factors affecting Heterorhabditis spp. such as humidity, temperature, pH, and light intensity.Keywords – Entomopathogenic Nematodes, Pathogenicity, Spodoptera litura

Sus sp. DNA encoding Cyt b gene detection test on soft gel candy samples using PCR method Latifatoel Chilmi; Tri Susilowati; Yuanita Rachmawati; Saiku Rokhim; Inggrit Tyautari
Journal of halal product and research (JPHR) Vol. 4 No. 1 (2021): Journal of Halal Product and Research (JHPR)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jhpr.vol.4-issue.1.14-19

Softgel candy is soft-textured confectionery processed by the addition of several components such as gum, pectin, starch and gelatin, to obtain a supple product and packed after aging treatment first. Gelatin is one of the main components in the manufacture of soft candy derived from the hydrolysis of collagen connective tissue and animal bone that serves as the nature of gelling agents, stabilizers or emulsifiers. However, the gelatin used in products not yet labeled halal Indonesian Council of Ulama (MUI) is particularly vulnerable to pork gelatin, since pork gelatin is cheaper than cattle. The purpose of this study was to test the contaminants of pig DNA on 17 samples of soft candles not labeled halal MUI. This research used Polymerase Chain Reaction (PCR) method. Seventeen samples were isolated by DNA, then spectrophotometry was performed, followed by PCR. The PCR product is run electrophoresis. Visualize the DNA with a UV gel documentation. Primer used is primer gene encoding cyt b DNA pork. Results showed that 17 samples were negative contaminants, while the positive control of pork showed a DNA band of 149 bp. This shows that Softgel Candy 17 samples do not contain pork gelatin.

KEANEKARAGAMAN BELALANG (ORTHOPTERA : CAELIFERA) PADA AREA PERSAWAHAN DI DESA SEKETI, KECAMATAN MOJOAGUNG, KABUPATEN JOMBANG Nur Qomariyah Pratiwi; Saiful Bahri; Saiku Rokhim; Ita Ainun Jariyah; Esti Tyastirin
BIO-EDU: Jurnal Pendidikan Biologi Vol 7 No 3 (2022): BIO-EDU: Jurnal Pendidikan Biologi Volume 7 Nomor 3 Tahun 2022
Publisher : Jurusan Pendidikan Biologi, Fakultas Ilmu Pendidikan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jbe.v7i3.2920

Rice field ecosystem is an artificial ecosystem that is used in agriculture. Rice fields in Seketi Village have vegetation types of corn, sugar cane, and rice, so that it will affect of grasshopper diversity in each vegetation. The purpose of this study was to determine the diversity of grasshoppers (Caelifera) and to determine the effect of vegetation on the diversity of grasshoppers in the rice fields of Seketi Village. The method used is purposive sampling and direct capture, namely research by making 5 plots measuring 5m x 5m with different types of plant vegetation (paddy, corn, sugar cane) catching with Sweep Net (insect nets). The results showed that there were species of Phlaeoba antennata, Phlaeoba fomusa, Oxya chinensis, Valanga nigricornis, Caryanda spuria, Atractomorpha crenulata, and Tettigidea sp. with a total of 152 individuals from 3 families. The diversity index value shows that plot 4 (maize plants) has the highest H' = 1.58 with 6 species of grasshoppers found and plot 1 (cane plants) has the lowest diversity index value of H = 1.09. Based on the differences in the value of grasshopper diversity in each plot caused by different types of vegetation, habitat conditions, and abiotic factors.

Struktur Komunitas Collembola Pada Habitat Gua Lowo dan Kebun Warga di Desa Melirang Kabupaten Gresik, Jawa Timur Ria Safitri; Siti Zulaikha; Saiful Bahri; Saiku Rokhim; Ita Ainun Jariyah; Nirmala Fitria Firdhausi
Jurnal Pro-Life Vol. 9 No. 3 (2022): November
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

Collembola is one of the soil fauna that acts as the smallest constituent component for the balance of the ecosystem. This study aims to compare the structure of the Collembola community in the habitat of Lowo Cave and Citizen's Garden in Melirang Village, Gresik Regency. This research uses descriptive exploratory research with soil drill method for soil sampling and pitfall trap for ground surface insect traps. Based on Collembola data obtained in Lowo Cave, there are 3 species with a total of 87 individuals, while in Citizen's Garden there are 6 Collembola species with a total of 145 individuals The results of the analysis of this study indicate that the Diversity Index value in the Lowo Cave habitat is H'= 0.859735 and in the Citizen's Garden a habitat with the value H' = 1.475675, the Evenness Index value in the Lowo Cave is E = 0.782565 and in the Citizen's Garden with a value of E = 0.82359, the value of the Dominance Index in Gua Lowo is 0.48104 and in the Citizen's Garden is 0.282949, the Relative Frequency of Ascocyrtus sp. and Hypogastrura consanguinea have a relative frequency of 100%.

Co-Authors Adzakiyyi, Miftahul Lathif AGUSTINA, EVA DAMAYANTI, MAMIK Dian Novitasari Erna Zahro’in Esti Tyastirin Faizah, Hanik Firmansyah, M. Aliffiyan Hakim, Ahmad Khairul Handayani, Ade Tiyan Hartono, Hermalya Putri Hawwa' Cahya Maulida Hermanto, M. Humayra Qurrata Aini Inggrit Tyautari Inggrit Tyautari Ita Ainun Jariyah Kautsar, Radinal Latifatoel Chilmi M. Aliffiyan Firmansyah Mauludin, Muchammad Firman Miftahul Lathif Adzakiyyi Misbakhul Munir Ninik Fadhillah Nirmala Fitria Firdhausi Nur Qomariyah Pratiwi Radinal Kautsar Ramadhani, Aisyah Hadi RIA SAFITRI Saiful Bahri Sari, Novita Mulya Siti Zulaikha Teguh Hari Sucipto, Teguh Hari Tri Susilowati Tri Susilowati Tri Susilowati Tyautari, Inggrit widya pertiwi Yosi Setia Prasiska Yuanita Rachmawati

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