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All Journal Jurnal Ilmiah Manuntung Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Kreativitas PKM Manuju : Malahayati Nursing Journal KESANS : International Journal of Health and Science
Nafila, Nafila
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health

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Journal : KESANS : International Journal of Health and Science

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Ethanol Extract of Fresh Peel of Balangkasua Fruit (Lepisanthes alata (Blume) Leenh) as an Alternative Reagent in the Identification of Trichuris trichiura Eggs Nafila, Nafila; Arsyad, Muhammad; Kartika Sari, Putri
Jurnal KESANS : Kesehatan dan Sains Vol 5 No 1 (2025): KESANS: International Journal of Health and Science
Publisher : Rifa'Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54543/kesans.v5i1.459

Abstract

Introduction: Soil-Transmitted Helminths (STH) remain a major public health concern, particularly in areas with limited sanitation. Conventional staining using 2% eosin, although effective, poses drawbacks due to its non-biodegradability, toxicity, and flammability. Objective: This study aimed to evaluate the potential of ethanol extract from the fresh peel of Balangkasua fruit (Lepisanthes alata (Blume) Leenh) as a natural alternative reagent for the identification of Trichuris trichiura eggs. Method: A laboratory-based experimental design was conducted using fecal samples containing STH eggs. The fruit peel extract was prepared through maceration with ethanol and tested at concentrations of 20%, 40%, 60%, 80%, and 100%, while 2% eosin served as the positive control. Staining quality was assessed based on egg color contrast, shell clarity, internal morphology, shape and size clarity, and background staining. Result and Discussion: Results showed that the 100% extract yielded the highest score (13), categorized as good staining quality, while lower concentrations (20%–80%) were classified as moderate. The positive control consistently achieved superior results (score 19, very good). Statistical analysis using the Kruskal–Wallis test revealed significant differences among treatment groups (p < 0.05). Conclusion: These findings demonstrate that L. alata peel extract possesses potential as an eco-friendly alternative stain, although further optimization of extraction methods, pigment stabilization, and validation across various STH species are necessary before replacing synthetic eosin in routine diagnostics
Co-Authors Al Medina, Namira Aulia Ramadhani Bandawati, Bandawati Haqiqi, Rizqia Nurul Jamaludin, Wahyudin Bin Kartika Sari, Putri Khaira, Dicky Septiannoor Kurniawan, Guntur Muhammad Arsyad Nurbidayah Prihandini, Yustin Ari Rahayu, Aesty Rahmat Hidayat Rahmi Muthia Ramdhani, Aulia Riyana, Adies Setia, Lisa Torizellia, Cast Wahyunita, Sari