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All Journal Jurnal Harpodon Borneo Journal of Environmental Engineering and Sustainable Technology Jurnal Penelitian Pendidikan IPA (JPPIPA) Berita Biologi Journal of Tropical Diversity
Kusuma, Wahyu Endra
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Materials Science & Nanotechnology Mechanical Engineering Physics

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KAJIAN MORFOMETRIK DAN NISBAH JENIS KELAMIN IKAN LEMPUK DI RANU GRATI, KABUPATEN PASURUAN, JAWA TIMUR Anitasari, Septi; Faqih, Abd Rahem; Kusuma, Wahyu Endra; Yuniarti, Ating
Jurnal Harpodon Borneo VOLUME 14 NO. 1 APRIL 2021
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v14i1.1898

Abstract

Ikan Lempuk merupakan salah satu komoditas perikanan yang menjadi salah satu sumber kebutuhan protein hewani masyarakat serta daya tarik wisatawan yang berkunjung ke Ranu Grati Pasuruan Jawa Timur Indonesia. Ikan Lempuk mudah ditemukan di Ranu Grati dan tidak ditemukan di tempat lain. Hasil yang diperoleh dari penelitian ini adalah ikan Lempuk Ranu Grati termasuk dalam genus Gobiopterus sp. Morfologi ikan ini dapat dikenali dari warna tubuhnya yang transparan dimana organ dalam seperti jantung, ginjal, kantung renang, pembuluh darah dan tulang belakang dapat dilihat dari luar tubuh. Ikan ini memiliki panjang rata-rata 2,43 cm, berat rata-rata 0,1223 gram. Bentuk badan fusiform, letak mulut sub terminal superior, bentuk sirip ekor truncate dengan tipe ekor diphycercal. Ikan ini memiliki gigi pada kedua rahang atau biasa disebut letak gigi vomer dan memiliki bentuk gigi canine, terdapat dua jenis bentuk ekor yaitu tipe Rounded dan Truncate.. Nisbah jenis kelamin lebih dominan pada jantan daripada betina, dengan perbandingan  6,2: 3,8.
MORPHOLOGICAL COMPARISON OF Hampala macrolepidota (Cyprinidae: Hampala) FROM SEVERAL POPULATIONS IN EASTERN JAVA Amalia, Elok; Harahap, Muhammad Alfiandi Rachmad; Sufaichusan, Ifa; Dailami, Muhammad; Widodo, Maheno Sri; Faqih, Abd Rahem; Kusuma, Wahyu Endra
Journal of Environmental Engineering and Sustainable Technology Vol 12, No 1 (2025)
Publisher : Directorate of Research and Community Service (DRPM)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The potential of freshwater fisheries in local species such as hampal fish (Hampala macrolepidota) presents several research gaps, particularly in specific localities. Research on H. macrolepidota in East Java may serve as a foundation for future management and development of local species, especially given that H. macrolepidota aquaculture is rarely carried out. Morphological studies based on meristics and morphometrics can be used for broodstock selection in H. macrolepidota domestication efforts. In this study, 21 Hampala specimens were assessed based on morphometrics and meristics, and analysed using a significance test, PCA and dendrogram. The analysis results by PCA and dendrogram showed morphometric differences in the Probolinggo and Banyuwangi populations compared with the four Brantas River populations. In contrast, based on meristics, the entire population does not show apparent differences based on the population. The clear differences demonstrated in the Probolinggo and Banyuwangi populations morphometrically indicate local adaptation due to differences in water conditions.
Analisis Karakteristik Morfologi dan Pola Pertumbuhan Ikan Uceng Nemacheilus fasciatus (Valenciennes, 1842) pada Sungai Setail, Banyuwangi, Jawa Timur Orchida, Kharisma; Arfiati, Diana; Kusuma, Wahyu Endra; Hamami, Syeftyan Muhammad Ali
Jurnal Penelitian Pendidikan IPA Vol 11 No 1 (2025): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i1.7446

Abstract

Indonesia's. Setail River is one of the longest rivers in Banyuwangi Regency. Setail River has high potential for fishery biodiversity. One of the fish that is often found in Setail River is Barred loach or known locally as Uceng Fish (Nemacheilus fasciatus). The purpose of this study was to determine the morphological characteristics and determine the relationship between length, weight and condition of Uceng fish in Setail River. Observation of color patterns and special characteristics of sample fish morphologically Nemacheilus fasciatus with characteristics including Having a brown body color, a small elongated body (fusiform). On the body there is a longitudinal black spot pattern, a dorsal pattern resembling a saddle, a homocercal tail fin type, an emarginate tail morphology (slightly concave). The type of mouth is subterminal inferior and has 3 pairs of barbels. Meristic observations of uceng fish have pectoral fins, pelvic fins, dorsal fins, tail fins and anal fins. In this study, an analysis of the relationship between length and weight showed that the growth pattern of the uceng fish was negative allometric and the condition factor obtained the range of condition factor values for male uceng fish during the study at each station for male uceng fish 0.68-1.35 and female uceng fish 0.68-1.40.
The Effect of Different Guanidino Acetic Acid (GAA) Levels and Protein Source on Blood Profile of Tilapia Fish (Oreochromis niloticus) Afifah, Fakhirah Oktafiani; Mahariawan, I Made Dedi; Kusuma, Wahyu Endra; Hidayat, Bagus R.; Yuniarti2, Ating; Hariati, Anik Martinah
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.7608

Abstract

Tilapia (Oreochromis niloticus) is a freshwater fish that has high economic value and potential to be cultivated. The problem that often arises in tilapia cultivation is the lack of optimal use of the feed given. The purpose of this research was to find out blood profile of tilapia during culture period. The method used was a completely randomized design (CRD) with five treatments and every treatment with four replications. This study used 240 Tilapia with average weight of 15 g. The stocking density of the aquaria is 15 fish. Test feed in this study is the form of dry pellets consisting combination of GAA levels, protein content (25% dry matter bases), and source of protein (fish meal and non-fishmeal). During the cultured period of 70 days, fish fed by formula diet of 3% biomass per day. Sampling for blood profile tested of erythrocytes, leukocytes, and hematocrit is carried out after the research. The water quality of temperature, pH, and DO were daily monitoring. The combination treatment of guanidino acetic acid (GAA) levels with different protein sources (fishmeal and non-fishmeal) had a significant effect on total erythrocyte of tilapia, but did not have significant differences between treatments on total leukosyte and hematocrit. Treatment with 1.2 g/kg GAA, fish meal protein source was the best treatment in this research.
Analisis Morfologi Integratif Ikan Berod (Mastacembelus unicolor) di Sungai Brantas, Jawa Timur Sufaichusan, Ifa; Astuti, Septiana Sri; Paricahya, Akhsan Fikrillah; Lestari, Bela Fatma Hani Ayu; Kholil, Kiki Nur Azam; Apriliyanti, Fisma Josara; Yuniarti, Ating; Widodo, Maheno Sri; Kusuma, Wahyu Endra
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.7978

Abstract

Mastacembelus sp., a member of the Mastacembelidae family, is distributed across Java, Sumatera, and Kalimantan. Mastacembelus, locally named berod fish in the Brantas River lacks population’s descriptions and morphological analyses. Morphological analyses, including morphometric and meristic descriptions, are important for precise identification and classification of kinship relationships, serving as a cornerstone in the fields of biology, taxonomy, and conservation. This study aimed to conduct a comprehensive morphometric and meristic identification of the berod fish population in the Brantas River, East Java. A total of 37 samples were collected from 6 different locations: Blitar, Tulungagung, Kediri1, Kediri2, Nganjuk, and Mojokerto. The morphometric measurements were based on 25 characters, while the meristic calculations were based on 6 characters. The data analyzed with PCA and CA using SPSS and PAST programs. The results revealed that berod fish in the Brantas River had morphological characters belongs to the Mastacembelus unicolor. However, notable disparities were observed in meristic characters, particularly in the Dorsal Fin Spines (DFS). PCA analysis showed significant differences in morphometric characters but not supported by meristic characters. These results underscore the importance of integration both morphological and meristic analyses for a comprehensive understanding of the berod fish population.
POSISI FILOGENETIK IKAN KOTES (Channa gachua (Hamilton, 1822)) DAN IKAN KUTUK (Channa striata (Bloch, 1793)) DARI JAWA TIMUR BERDASARKAN URUTAN DNA MITOKONDRIA CYTOCHROME C OXIDASE SUBUNIT I Kusuma, Wahyu Endra; Widyawati, Yuni; Dailami, Muhammad; Kholil, Kiki Nur Azzam; Paricahya, Akhsan Fikrillah; Sufaichusan, Ifa; Wiadnya, Dewa Gede Raka
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.2733

Abstract

Ikan kotes (Channa gachua) dan ikan kutuk (Channa striata) adalah spesies dari famili Channidae yang tersebar secara alami di perairan Indonesia. Kedua spesies tersebut memiliki nilai ekonomi yang tinggi karena beberapa alasan, yaitu sebagai sumber protein bagi masyarakat setempat, memiliki kandungan albumin yang penting bagi kesehatan manusia, serta belakangan ini menjadi populer sebagai ikan hias. Penelitian ilmiah dasar seperti studi filogenetik penting untuk dilakukan guna mendukung upaya domestikasi maupun konservasi. Di penelitian ini, kami melakukan koleksi spesimen C. gachua dan C. striata dari beberapa lokasi berbeda di Jawa Timur untuk menjelaskan posisi filogenetiknya di antara spesies lain dari genus Channa. DNA mitokondria gen sitokrom c oksidase subunit I (COI) dari satu perwakilan individu untuk setiap lokasi telah diurutkan dengan metode sequencing. Dalam melakukan analisis filogenetik, sekuens DNA untuk banyak spesies dari genus Channa diperoleh dari penelitian sebelumnya. Pohon filogenetik maximum likelihood menunjukkan bahwa individu C. striata dari Jawa Timur mengelompok bersama dan membentuk clade dengan nilai bootstrap yang sangat tinggi (100%). Sebaliknya, C. gachua dari Jawa Timur berkerabat jauh dengan C. gachua dari Jawa Barat dan Kanchanaburi, Thailand, dengan divergensi genetik yang tinggi dengan jarak genetik > 2%. Hasil ini selaras dengan penelitian sebelumnya yang menunjukkan bahwa C. gachua merupakan spesies kriptik yang terdiri dari dua spesies atau lebih. Penelitian lebih lanjut perlu dilakukan, misalnya mengenai keragaman genetik, struktur populasi, dimana data dapat digunakan untuk merancang manajemen konservasi atau program pemuliaan yang efektif untuk kedua spesies tersebut.
Analisis Identitas Genetik Ikan Gabus Sentani (Oxyeleotris heterodon) berdasarkan Marka Gen Sitokrom Oksidase 1 Dailami, Muhammad; Maulana, Antajala Rian; Siahaan, Marthin Daniel Theo Gratia; Kusuma, Wahyu Endra; Agamawan, Lalu Panji Imam; Toha, Abdul Hamid A.; Yuniarti, Ating
Journal of Tropical Diversity Vol 1 No 1 (2025): Februari
Publisher : Penerbit Brainy Bee

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.64283/jotdiverse.2025.1(1):4

Abstract

The Sentani snakehead fish (Oxyeleotris heterodon) is an endemic fish species that inhabits Lake Sentani and several other lakes in Papua Province, Indonesia. This species has important ecological and economic value, but is vulnerable to environmental change and overexploitation. Genetic identification, especially using the Cytochrome Oxidase 1 (COI) gene, is very important for conservation efforts and understanding the genetic diversity of Sentani Snakehead Fish. DNA barcoding techniques with the COI gene have become the standard method in molecular identification of species, allowing rapid and accurate identification. Unfortunately, the Sentani Snakehead Fish faces various threats such as habitat degradation, pollution, overfishing and the introduction of invasive foreign species. Therefore, genetic research is becoming increasingly important to support conservation efforts, providing in-depth understanding of the population status and adaptive potential of species. The results of DNA amplification and sequencing of COI gene fragments showed a DNA sequence with a length of 619 base pairs with the composition A (23.9%), G (18.1%), C (29.1%), T (28.9%). The results of identification by homology analysis in the NCBI and BOLD databases showed the highest similarity at 97.58% and 97.84% with the O. lineolate sequence. This is because there is no O. heterodon sequence from the COI gene fragment found in Genbank or the BOLD system. Phylogenetic tree analysis with several sequences that have close similarities shows a difference in clade between the O. heterodon sequence from this study with the O. lineolate sequence and other species.
Co-Authors Abdul Hamid A. Toha Abdul Rahem Faqih Afifah, Fakhirah Oktafiani Amalia, Elok Apriliyanti, Fisma Josara Ating Yuniarti Dewa Gede Raka Wiadnya Diana Arfiati Hamami, Syeftyan Muhammad Ali Harahap, Muhammad Alfiandi Rachmad Hariati, Anik Martinah Hidayat, Bagus R. Kholil, Kiki Nur Azam Kholil, Kiki Nur Azzam Lalu Panji Imam Agamawan Lestari, Bela Fatma Hani Ayu Mahariawan, I Made Dedi Maheno Sri Widodo, Maheno Sri Maulana, Antajala Rian Muhammad Dailami, Muhammad Orchida, Kharisma Paricahya, Akhsan Fikrillah Septi Anitasari, Septi Septiana Sri Astuti, Septiana Sri Siahaan, Marthin Daniel Theo Gratia Sufaichusan, Ifa Widyawati, Yuni Yuniarti2, Ating