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Perkembangan Telur Cacing Haemonchus contortus Menjadi Larva Stadium Pertama pada Media Air Secara In Situ I Gusti Komang Oka Wirawan; Ni Sri Yuliani; Novianti Neliyani Toelle; . Suryawati
Buletin Veteriner Udayana Vol. 14 No. 6 December 2022
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2022.v14.i06.p20

Abstract

Haemonchus contortus is a highly pathogenic worm in sheep and goats. A study was conducted to determine the in situ percentage of the development of H. contortus worm eggs at the first-stage larvae (L1) in water. This research treatment was repeated 5 times in one group with a duration of 10 days and each group used 8 female H. contortus worms. These worm eggs are counted using the McMaster method. The prepared worm samples were ground, immersed in 5 mL of pro-injection aqua (pH 7.0), and filtered using a tea filter. Research procedure: 1.5 mL of suspension was collected, placed into a tube and treated according to the procedure. Each tube, covered with aluminium foil that has holes 15 to 20, was placed on pasture for 48 h (05:00 – 24:00); where ambient temperature was recorded every four hour. Research variable include: percentage of hatched worm eggs in the immersion group. The hatchability percentage = ((the difference between numbers of worm eggs before and after treatment): (those before treatment) x 100)). Data were tabulated and analysed descriptively. The results showed that: the percentage of the total average development of the L1 H. contortus worm eggs was 26.5%. Conclusion: the percentage of egg development of H. contortus in situ was 26.5%. The influencing factors are ambient temperature, pH, humidity, culture media, and egg abnormalities. It is recommended to extend the breeding time in the media in the hope that the percentage yield is more optimal.
MORTALITAS LALAT MUSCA DOMESTICA YANG DIBERI ESKTRAK METANOL CHROMOLAENA ODORATA Gerson Y. I. Sakan; Ni Sri Yuliani; Yosefus F. da Lopez; I Gusti Komang Oka Wirawan
Partner Vol 28, No 1 (2023): Edisi Juli 2023
Publisher : Politeknik Pertanian Negeri Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35726/jp.v28i1.1539

Abstract

This study aimed the methanol extract of the leaves of Chromolaena odorata against Musca domestica flies. The research design used a completely randomized design with 4 treatments with 5 replications. Each experimental unit contained 20 flies. The tested extracts were divided into concentrations of 10% (P1), 20% (P2), 30% (P3), and 10% soap solution was applied by spray. The variables observed were flies that died after 12 hours of exposure to the extract. The data obtained was tested with ANOVA, if there was a significant difference, it was continued with the Duncan test. Based on the statistical results showed that there was a very significant difference between treatments (P<0.01). Duncan's test showed there was a significant difference in mortality of P4 with mortality of P1, P2, and P3 where P1, P2, and P3 gave the same effect on mortality of flies 89-100%. Thus the concentration of P1, P2, and P3 is better.Key Words:  Mortality, Musca domestica, Methanol extract, Chromolaena odorata   
Tingkat Infeksi Trichuris Ovis pada Musim Kemarau di Laboratorium Lapangan Politani Kupang I Gusti Komang Oka Wirawan; Agustinus Semang; Aholiab Aoetpah; Suryawati .
Buletin Veteriner Udayana Vol. 15 No. 6 December 2023
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2023.v15.i06.p17

Abstract

The goats being raised semi intensively on the field laboratory of State Agricultural Polytechnic of Kupang were kacang goats (Capra hircus). Objective: to measure Trichuris ovis infectious levels based on age during dry season. Nine heads of goats were grouped into two of four for 6 to 7 and five for 18 to 24 month old. Two methods to identify morphological worm eggs are sedimentation and floating; while infectious intensity was using McMaster method. Faecal samples for the two groups were collected twice a week. Descriptive analysis was applied to determine gastrointestinal endoparasites and endoparasite infectious intensity, so that of ambient temperature of pasture. The results showed that average infectious intensity of Trichuris ovis worm eggs for 6 to 7 month old goats was 100 TTG while that for 18 to 24 month old was 200 TTG. It can be concluded that the intensity of Trichuris ovis infection in goats aged 18 to 24 months is higher than that aged 6 to 7 months and is included in the light infection category. This infection category is influenced by season and food source. The difference in the age of the goats is one of the factors causing the difference in the intensity of Trichuris ovis infection between the two groups. The author suggests that in further Res. it would be better to use a larger number of samples with a longer Res. time so that the results are more accurate.