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Identifikasi Taenia solium secara Mikroskopis pada Peternakan Babi Bekti, Heri Setiyo; Habibah, Nur; Rinawati, Luh Putu; Pradnya Yasa, Ni Putu Candra Dewi; Rindi, Oktavelendi Dhaneta Graha; Dewi, Ni Km Ayu Kusuma; Savitri, Ni Putu Ayu Dani; Rakhmawati, Aprilia
Jurnal Kesehatan Vol 12, No 1 (2021): Jurnal Kesehatan
Publisher : Politeknik Kesehatan Tanjung Karang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jk.v12i1.2448

Abstract

Taenia solium is a zoonosis found all over the world, especially in developing countries. T solium can cause taeniasis and cysticercosis in humans. Pigs as intermediate hosts can be infected with T solium which causes porcine cysticercosis. T solium can infect muscle, skin, eye, and brain tissue which can develop into neurocysticercosis. Neurocysticercosis is the cause of 30% of cases of epilepsy in the world. In Denpasar city, pigs are one type of livestock. A large number of pig farms and the Balinese’s people's habit of consuming pork is one of the sources of T solium infection. This research was conducted to determine T solium eggs in pig feces. The study was conducted microscopically on 31 samples with simple random sampling technique. The results showed that of the 31 samples examined, 54.8% (17 samples) were positive for T solium eggs. This indicates that the pig farms in Denpasar city have been infected with T solium. Pig farming in Denpasar city was done traditionally and kept indoors. However, the breeders pay less attention to pig health and environmental sanitation. Also, pigs are slaughtered privately by breeders because there are no slaughterhouses. Lack of supervision of pork circulating in the community is also the cause of T solium infection. Therefore, it is necessary to carry out supervision by the local government on pig farms as well as supervision of pork consumed by the public to prevent and eradicate T solium infection.
Overview of Visfatin Levels in Pregnant Women as a Stunting Biomarker Ni Nyoman Astika Dewi; Luh Putu Rinawati; Ni Putu Ditya Prabandari Dewi; I Made Krishna Paramartha; Ni Putu Intan Maha Ayu Diyanti; Aprilia Rakhmawati; Heri Setiyo Bekti
JURNAL KEBIDANAN Vol 12, No 1 (2022): April 2022
Publisher : Politeknik Kesehatan Kementerian Kesehatan Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkb.v12i1.8018

Abstract

Stunting is one of the malnutrition problems experienced by toddlers in the world. Indonesia is the third country in the prevalence of stunting in the Southeast Asian region. Visfatin is one of the molecules that play a role in fetal development during the womb. Reports related to visfatin in pregnant women are still very few, and mention visfatin plays a role in the incidence of preeclampsia (PE), fetal growth restriction, and gestational diabetes mellitus (GDM). This study aims to determine the level of visfatin in pregnant women as a biomarker of stunting in Bangli Regency, Bali Province. This research is a descriptive study, with an observational design. Blood samples were taken from the median cubital vein. Samples were examined by ELISA method. In the examination of vistafin levels in 30 pregnant women, the lowest level being 0.53 ng/ml and the highest level being 56.72 ng/ml. From the results obtained and compared with previous studies, the visfatin levels obtained were lower than the visfatin levels in normal pregnant women. Many studies have shown that increased levels of visfatin in maternal plasma are associated with low birth weight and intrauterine growth restriction (IUGR). Babies born with LBW have a risk of stunting.
Daya Hambat Ekstrak Daun Sirih dan Daun Legundi Terhadap Pertumbuhan Bakteri Staphylococcus aureus Ida Bagus Oka Suyasa; Heri Setiyo Bekti; Luh Putu Rinawati; Luh Putu Laksmi; Putu Diah Wahyuni; Desak Gede Dwi Agustini; Aprilia Rakhmawati
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 5, No 1 (2022): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v5i1.11015

Abstract

Staphylococcus aureus is one of the causes of infectious disease, ranging from mild to severe infections such as meningitis. The drug that is often used is the penicillin class of antibiotics. Apart from chemical antibiotics, natural antibacterial compunds from plants can be also used as an alternative treatment for this infection. Such as green betel (Piper betle L.) and legundi (Vitex trifolia L.). The ethanol extract of betel leaf has antibacterial activity against Staphylococcus aureus. The phytochemical content in legundi leaf is also able to inhibit the growth of Staphylococcus aureus. The ethanol extract of betel leaf and legundi is made through a maceration process. The extract concentrations used in this study were 20%, 30%, and 40%. The inhibition test of Staphylococcus aureus using the disc method. From the results of this study, the active substances found in betel leaf were flavonoids, tannins, and phenols, while in legundi were found flavonoids, tannins and phenols, and quinones. In total, the mean inhibition zone in this study ranged from 7.87mm to 17.33mm where legundi leaf extract was in the moderate category, betel and combination extract were classified as strong. There was a difference in the diameter of the inhibition zone of betel and legundi extract at a concentration 20% with 40%. In the combination extract there is a difference in the concentration of 20% with 40% and 30% with 40%. An antimicrobial compound can be said to synergize if a mixture of two or more antimicrobial compounds is able to provide an effect of each compounds. In this study, it has seen that the mean of the combination is higher than the mean of legundi leaf extract. Meanwhile, the mean of betel leaf extract is still greater than the combination extract.
Gambaran Kadar Glukosa dan Total Kolesterol pada Wanita Hamil di Kabupaten Bangli, Bali: An Overview of Glucose Levels and Total Cholesterol Levels among Pregnant Women in Bangli District, Bali Heri Setiyo Bekti; Ni Nyoman Astika Dewi; Luh Putu Rinawati; Luh Ade Wilankrisna; I Made Suarjana; I Made Yogi Hardiyanta; Ni Putu Lisa Anjani; Aprilia Rakhmawati
Jurnal Bidan Cerdas Vol. 4 No. 2 (2022)
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/jbc.v4i2.1024

Abstract

Introduction: Gestational diabetes mellitus (GDM) is a state of glucose intolerance that was first detected during pregnancy. GDM is increasing worldwide, it is estimated that 15% to 22% of all pregnancies are affected by GDM. GDM can be associated with many consequences, such as fetal macrosomia, preeclampsia, and high caesarean rates. The purpose of this study was to determine the description of glucose and total cholesterol levels in pregnant women in Bangli Regency, Bali. Methods: This research method is descriptive by checking glucose and total cholesterol levels using the enzymatic-colorimetric method. The subjects in this study were 33 pregnant women, with sampling method using the probability sampling method, with a simple random sampling technique. Results: The result of this study is, glucose levels in all samples were in the normal category, while for total cholesterol levels 48,48% were in the normal category and 42,42% were in the abnormal category. Conclusion: Normal glucose levels can be caused by several factors such as the age of the respondent under 40 years, gestational age at term, and the hormones that regulate blood glucose in the body functioning properly. An increase in cholesterol levels can be caused by an increase in sexsteroid hormones, as well as changes in liver and adipose tissue metabolism. An increase in total cholesterol levels in pregnant women needs to be watched out for because it can be on of the markers of the incidence of GDM.   ABSTRAK Pendahuluan: Diabetes melitus gestasional (DMG) merupakan keadaan intoleransi glukosa yang pertama kali terdeteksi selama kehamilan. DMG meningkat di seluruh dunia, diperkirakan 15% sampai 22% dari semua kehamilan dipengaruhi oleh DMG. DMG dapat dikaitkan dengan banyak konsekuensi, seperti makrosomia janin, preeklampsia, dan tingkat operasi caesar yang tinggi. Tujuan dari penelitian ini adalah untuk mengetahui gambaran kadar glukosa sewaktu dan kolesterol total pada ibu hamil di Kabupaten Bangli, Bali. Metode: Metode penelitian ini adalah deskriptif dengan memeriksa kadar glukosa sewaktu dan kolesterol total menggunakan metode enzimatik kolorimetri. Subyek pada penelitain 33 ibu hamil, dengan cara penarikan sampel dengan mengunakan metode probality sampling, dengan teknik simple random sampling. Hasil: Hasil dari penelitian ini, diperoleh kadar glukosa sewaktu seluruh sampel berada pada kategori normal sedangkan untuk kadar kolesterol total sebanyak 48,48% berada pada kategori normal dan 42,42% pada kategori abnormal. Kesimpulan: Kadar glukosa yang normal dapat disebabkan oleh usia repsonden di bawah 40 tahun, usia kehamilan yang cukup bulan, dan hormon pengatur glukosa yang berfungsi dengan baik. Peningkatan kadar kolesterol total dapat disebabkan oleh peningkatan hormon steroid seks, perubahan metabolisme hati dan jaringan adipose. Peningkatan kadar kolesterol total pada ibu hamil perlu diwaspadai karena dapat menjadi salah satu penanda kejadian DMG.
Gambaran Kadar Hemoglobin Dan Protein Urin Pada Ibu Hamil Luh Putu Rinawati; Ni Putu Ditya Prabandari Dewi; Ni Putu Yulia Rahma Dewi; Ni Komang Omik Trianita Udiana; Ni Wayan Rika Kumara Dewi; Heri Setiyo Bekti
Meditory : The Journal of Medical Laboratory Vol 10, No 2 (2022): Meditory, Volume 10 No. 2 Tahun 2022
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v10i2.2279

Abstract

Backgrounds of hemoglobin is a metalloprotein in red blood cells that functions as an oxygen booster from the lungs to the rest of the body. To maintain hemoglobin levels in the body, especially in pregnant women, it is necessary to have adequate intake of nutrients, namely iron. If the intake of iron in pregnant women is less, it will increase the risk of anemia. Anemia is a condition or condition that causes low levels of hemoglobin (Hb). Albuminuria or proteinuria is a condition in which urine or urine contains an abnormal amount of albumin. Throughout pregnancy, pregnant women are usually advised to take a urine test. One of the focuses of the urine test is to determine urine protein levels in pregnant women. In pregnant women, there is a phase where urine protein levels increase, namely, lack of fluids, exposure to cold and extreme weather, new strenuous activities, and emotional stress also often causes urine protein levels in pregnant women to increase, and impaired kidney function. or urinary tract infection. The method we used in this research is descriptive research method to describe the levels of hemoglobin (Hb) and urine protein in pregnant women at the Ubud 1 Public Health Center, Gianyar-Bali. With the sampling technique using the probability sampling method, with a simple random sampling technique. The results of 41 respondents, that pregnant women who have normal Hb levels (≥ 11 g/dL) at Puskesmas Ubud 1 were found to be more than pregnant women who have low Hb levels ( 11 g/dL). And from the total sample, as well as 2 pregnant women from the urine protein level examination data were positive. The conclusion after averaging the Hb and urine protein levels of pregnant women at the ubud 1 health center that it can be said that the health condition of pregnant women is quite good, but there are still some respondents who show results that are not in accordance with normal limits, therefore it is important to maintain health and pattern healthy living.Keywords: hemoglobin, urine protein, pregnant women
ANALISIS SISTEM PENGENDALIAN INTERNAL PENERIMAAN BARANG PADA HOTEL PANDAWA ALL SUITE Ni Luh Putu Rinawati; Putu Aristya Adi Wasita
Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Vol. 6 (2023): PROSIDING SINTESA
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/snts.v6i.2772

Abstract

Setiap perusahaan tentunya perlu menerapkan prinsip - prinsip sistempengendalian internal sehingga dalam kegiatan operasionalnya agar dapatberlangsung secara efektif dan efisien baik dari segi waktu, biaya dan tenaga. HotelPandawa All Suite merupakan perusahaan di bidang jasa akomodasi yang secaraumum sudah menerapkan sistem pengendalian internal secara baik. Adapun masalahyang ditemukan yaitu, pada bagian receiving sering terjadi barang yang diterimajumlah dan jenisnya tidak sesuai dengan purchasing order sehingga menghambatkegiatan operasional hotel. Penelitian ini bertujuan untuk mengetahui apakah sistempengendalian internal penerimaan barang pada Hotel Pandawa All Suite sudahmenerapkan prinsip-prinsip pengendalian internal yang baik. Jenis penelitian yangdigunakan adalah penelitian deskriptif kualitatif. Data yang digunakan adalah dataprimer dan sekunder. Hasil dari penelitian ini menunjukkan bahwa sistempengendalian internal pada proses penerimaan barang sudah berjalan dengan baiknamun masih memiliki kelemahan seperti, bagian receiving yang tidak menggunakansurat order pembelian sebagai dasar penerimaan barang yang menyebabkan seringterjadi barang yang diterima jumlah dan jenisnya tidak sesuai dengan purchasingorder/daily market list.
The Effect of Green Grape Extract on Serum Triglyceride Levels in Rats: Pengaruh Pemberian Ekstrak Anggur Hijau terhadap Kadar Triglierida Serum pada Tikus Bekti, Heri Setiyo; Dewi, Ni Nyoman Astika; Rinawati, Luh Putu; Dewi, Ni Wayan Rika Kumara; Hadi, Mochammad Choirul
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 7 No. 1 (2024): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v7i1.1721

Abstract

Cardiovascular disease, one of which is coronary heart disease (CHD) is the main cause of death and morbidity caused by blockages in the coronary arteries (atherosclerosis). Elevated serum triglyceride levels can encourage the formation of atherosclerosis. Statins are the drugs most commonly used to lower triglyceride levels but have side effects, so new treatment are needed. Grapes are fruits that has many bioactive compounds such as proanthocyanidins and resveratrol which are believed to reduce triglyceride levels in serum. The purpose of this study was to determine the effect of green grape extract (Vitis vinifera) on triglyceride levels in male Wistar rats which were divided into 5 groups, namely P0 (fed with standard feed), P1 (fed with high cholesterol and then standard feed), P2 (fed with high cholesterol and simvastatin 0.2mg/200mgBW/day), P3 (fed with high cholesterol and grape extract at a dose of 500mg/200mgBW/day), and P4 (fed with high cholesterol and 250mg/200mgBW/day grape extract). Examination of triglyceride levels was carried out using the GPO-PAP method. Data analysis was performed with Annova test followed by Bonferroni test. The statistical test results showed that there were significant differences between each treatment group. The P3 group showed a lower average triglyceride level than P4 although it was still higher than P2. This showed that administration of green grape extract has an effect on decreasing serum triglyceride levels in all.
Identifikasi Cacing Pita (Taenia solium) dengan Metode Mikroskopis dan Nested PCR Rinawati, Luh Putu; Yanty, Jannah Sofi; Kurniawan, Surya Bayu; Bekti, Heri Setyo; Antarini, Anak Agung Nanak; Puryana, I Gusti Putu Sudita; Dewi, Pande Putu Ayu Patria; Rakhmawati, Aprilia
Jurnal Analis Kesehatan Vol. 13 No. 1 (2024): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v13i1.4531

Abstract

Taenia solium merupakan parasit yang dapat menimbulkan infeksi taeniasis dan sistisekosis. Di Indonesia terutama di Bali kejadian taeniasis tinggi yang disebabkan kebiasan masyarakatnya mengonsumsi “lawar pork”. Daging babi yang terinfeksi dapat menjadi sumber penularan T. solium. Tujuan dari penelitian ini adalah untuk mengidentifikasi T. solium menggunakan metode mikroskopis dan Nested PCR. Penelitian merupakan penelitian deskriptif dengan desain observasional yang dilakukan terhadap 26 sampel yang diperiksa secara mikroskopis dan molekuler menggunakan Nested PCR dengan primer spesifik terhadap gen Tso31. Dari hasil penelitian menggunakan metode mikroskopis diperoleh sebanyak 21 sampel yang positif yang mengandung bagian cacing dan telur cacing, sedangkan dengan metode Nested PCR diperoleh 2 sampel positif yang memiliki berat molekul 234 bp. Identifikasi T. soliumpada manusia penting karena diperlukan untuk memberikan pengobatan tepat waktu pada penderita sekaligus untuk memutus rantai penularan parasit tersebut.
Primers application with the Tso31 gene target in the molecular identification of Taenia solium Habibah, Nur; Bekti, Heri Setiyo; Kumara Dewi, Ni Wayan Rika; Rinawati, Luh Putu; Burhannuddin, Burhannuddin; Rakhmawati, Aprilia
Muhammadiyah Medical Journal Vol 2, No 1 (2021): Muhammadiyah Medical Journal (MMJ)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.651 KB) | DOI: 10.24853/mmj.2.1.35-40

Abstract

Background: taeniasis is a zoonotic disease caused by Taenia spp. Human taeniasis caused by Taenia solium can be acquired after consumption of raw insufficiently cooked infected pork meat. Pigs are intermediate host for T.solium. Pigs acquired this infection by eating human feces that contained T.solium eggs. Pigs infected with T.solium can be transmitted to humans. Purposes: identification of T.solium in pig is important because it is indicator of T.solium transmission. Microscopic examination of T.solium eggs is considered less effective and efficient so that many other methods are developed for T.solium detection such as molecular and immunology. Method: This method used specific primer which can detect the Tso31 gene in T.solium. Tso31 gene is one of the most promising antigens to differentiate T.solium from T.saginata. Pig feces samples were taken by random sampling technique from 7 pig farms in Denpasar. Result: from the 30 samples, we found one sample that which gave a single amplification product of 234 bp. This indicates that the pig farms in Denpasar have been infected with T.solium. Conclusion: it is necessary to do meat inspection properly in the market as well as health education about the dangers and impacts of T.solium infection in the community.
PENYULUHAN KECACINGAN DAN PEMERIKSAAN KADAR Hb PADA ANAK SEKOLAH DASAR No1 DI DESA SAYAN UBUD GIANYAR BALI TAHUN 2023 Swastini, I Gusti Agung Ayu Putu; Habibah, Nur; Rinawati, Luh Putu
Jurnal Pengabmas Masyarakat Sehat Vol 6, No 1 (2024): JANUARI
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/jpms.v6i1.3096

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Anemia merupakan salah satu masalah kesehatan yang harus dihadapi Indonesia sampai saat ini. Pemerintah telah mencanangkan berbagai program penanggulangan anemia, termasuk pada remaja putri, tetapi prevalensi anemia masih tinggi. Salah satu penyebab turunnya kadar hemoglobin (Hb) adalah infeksi kecacingan, melalui perdarahan saluran cerna dan penurunan asupan makanan. Berdasarkan hasil survei pemeriksaan tinja pada anak SD di 10 kabupaten/kota di Indonesia, tahun 2011 diketahui dari sekitar 3.666 siswa di 64 SD, sekitar 829 anak mengidap cacingan atau prevalensinya sekitar 22,6 persen. (Kemenkes, 2012). Infeksi cacing masih merupakan masalah kesehatan di Indonesia. Infeksi ini merupakan salah satu penyebab anemia defisiensi besi pada anak usia sekolah dasar. Tujuan Pengabdian masyarakat ini untuk melakukan penyuluhan kecacingan dan pemeriksaan kadar Hb. Metode yang digunakan adalah penyuluhan, pre tes dan pos tes serta pemeriksaan kadar Hb pada anak Sekolah Dasar No 1 Sayan Ubud. Hasil penelitian ini adalah ada peningkatan hasil dari pre tes ke pos tes yaitu sangat baik dari 12 orang menjadi 26 orang, sedangkan yang kurang dari 24 orang menjadi 8 orang , sedangkan kadar Hb rata-rata adalah normal 81 orang, sedangkan kurang 4 orang. Secara umum untuk kadar Hb dan tingkat pengetahuan adalah normal dan baik. Kesimpulan. Penyuluhan tentang kecacingan dan pemerriksaan kadar Hb di SDN no 1 Ubud dengan tingkat pengetahuan rata-rata baik setelah dilakukan penyuluhan, seangkan untuk kadar Hb rata-rata normal, hanya 4 orang dibawah normal.