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All Journal Jurnal AGROTEKNOLOGI agriTECH Indonesian Food Science and TechnologyJournal Jurnal Penelitian Sains dan Teknologi Indonesia Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember
Masahid, Ardiyan Dwi
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Industrial & Manufacturing Engineering Social Sciences

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AKTIVITAS ANTIOKSIDAN HIDROLISAT PROTEIN IKAN WADER (Rasbora jacobsoni) DARI HIDROLISIS OLEH ENZIM CALOTROPIN DAN PAPAIN Witono, Yuli; Maryanto, Maryanto; Taruna, Iwan; Masahid, Ardiyan Dwi; Cahyaningati, Kinanti
JURNAL AGROTEKNOLOGI Vol 14 No 01 (2020)
Publisher : Faculty of Agricultural Technology, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/j-agt.v14i01.14817

Abstract

The potency of common barb fish as fish protein hydrolyzate caused by its high protein content. Fish protein hydrolyzate is produced through the hydrolysis process by the calotropin enzyme and the papain enzyme. This study used common barb fish with one type of treatment, i.e. the difference combination of the calotropin (C) and papain (P) enzyme. Making fish protein hydrolyzate is used to determine the antioxidant activity of the common barb fish protein hydrolyzate. The observation parameters in this study were enzyme activity, antioxidant activity, degree of hydrolysis, molecular weight, amino acids, dissolved protein content, and water holding capacity. Samples with a ratio of 40C: 60P enzyme concentration are wader fish protein hydrolyzate with the proportion of 40% calotropin enzyme and 60% papain enzyme is the highest sample that has an antioxidant activity of 36.41%, reduced power value of 0.57, hydrolysis degree of 74.40% and total amino acids of 82.79%. Wader fish hydrolyzate in the 70C: 30P sample of 76.98% has a high water holding capacity because it can maintain the protein component in meat. Keywords: antioxidant, calotropin, common barb fish, fish protein hydrolyzate, papain
Characteristics of Sardinella Smart Flavor based on Enzymatic Hydrolysis Using a Calotropin and Papain Combination Witono, Yuli; Masahid, Ardiyan Dwi; Belgis, Maria; Nafi', Ahmad; Azkiyah, Lailatul; Wahyuni, Livia; Majid, Nur Ika Okta
Indonesian Food Science and Technology Journal Vol. 7 No. 2 (2024): Volume 7. Number 2, July 2024 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v7i2.34095

Abstract

Sardinella lemuru is one type of fish with the highest population and the most widely caught fish in Indonesia. The protein content is very high, namely 17.8-20% contains various amino acids including glutamic acid, which can potentially be a smart flavor precursor. The research focuses on enzymatic smart flavor production by examining the effect of concentration and hydrolysis time of calotropin and papain combination on characteristics of the smart flavor produced and determining the best treatment to produce smart flavor. This research used two factors: the concentration of enzyme (3%, 4%, and 6% with a ratio of papain and calotropin of 70%: 30%) and hydrolysis time (1, 2, and 3 hours). The research showed that yield ranged from 50.15-50.69%; brightness 89.72-91.57; water 6.91-9.51%; soluble protein 1.13-1.56 mg/ml; antioxidant activity 48.40-54.47%; emulsion power 0.16-0.18 m2/g, emulsion stability 17.27-24.33 minutes; water holding capacity 83.44-89.27% and effectiveness 1.00 (enzyme 6% and time of hydrolysis 3 hours). The best treatment with effectiveness was Sardinella lemuru smart flavor (6% enzyme and 3 hours hydrolysis) with a yield 50.54%, brightness 89.72, water 6.91%, soluble protein 1.56 mg/ml, antioxidant activity 54.47%, emulsion power and stability 0.18 m2/g and 24.33 minutes, and water holding capacity 89.27%.
PERBAIKAN KUALITAS DAN INOVASI PRODUK MINUMAN HERBAL INSTAN PADA INDUSTRI RUMAH TANGGA NURFAIDAH DI DESA ANDONGSARI KECAMATAN AMBULU KABUPATEN JEMBER Masahid, Ardiyan Dwi; Belgis, Maria; Nafi', Ahmad
Jurnal Hasil Pengabdian kepada Masyarakat Universitas Jember Vol 3 No 1 (2024): Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember
Publisher : LP2M Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpmunej.v3i1.1144

Abstract

Minuman herbal serbuk instan merupakan produk bahan minuman berbentuk serbuk atau granula yang biasa dibuat dari gula dan rempah-rempah yang dicampur menjadi satu dengan atau tanpa penambahan bahan makanan lain dan bahan tambahan makanan. Beberapa bahan dasar yang digunakan dalam pembuatan minuman herbal serbuk instan adalah rempah-rempah dari spesies Zingiberaceae khususnya jahe, kunyit, temulawak dan kencur yang banyak ditemukan di Indonesia dan biasa digunakan sebagai obat tradisional. Salah satu UMKM di Dusun Watukebo Desa Andongsari Kecamatan Ambulu yang memiliki keterampilan dalam memanfaatkan hasil alam (tanaman) untuk kebutuhan hidup dan kesehatan. UMKM tersebut mengolah kunyit menjadi minuman herbal serbuk instan. Berdasarkan hasil observasi dan diskusi langsung dengan mitra didapatkan beberapa permasalahan yang dihadapi yaitu pengemasan dan desain kemasan kunyit instan yang sudah diproduksi masih sederhana dan masih belum ada labelnya serta kurangnya inovasi produk minuman herbal instan. Hasil dari pengabdian ini adalah penggunaan jenis dan desain kemasan yang lebih bagus dan menarik, sesuai dengan produknya, pengajuan SPP-IRT (Sertifikat Produksi Pangan Industri Rumah Tangga), praktek dan inovasi produk minuman herbal instanselain kunyit, yaitu jahe instan dan temulawak instan, serta penyerahan alat produksi dari tim pengabdian kepada mitra yaitu mesin penggiling rempah, mesin pres plastik/sealer, wajan stainless steel dengan tujuan dapat memproduksi dalam jumlah besar sehingga memasarkan produk dengan jangkauan yang luas.
PELATIHAN PEMBUATAN SAMBAL PECEL INSTAN DI KAWASAN PONDOK PESANTREN RADEN RAHMAT SUNAN AMPEL Witono, Yuli; Belgis, Maria; Masahid, Ardiyan Dwi; Nafi', Ahmad; Azkiyah, Lailatul; Wahyuni, Livia
Jurnal Hasil Pengabdian kepada Masyarakat Universitas Jember Vol 3 No 2 (2024): Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember
Publisher : LP2M Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpmunej.v3i2.4339

Abstract

Kelompok Riset (Keris E-Food) Universitas Jember bekerja sama dengan Pondok Pesantren Raden Rahmat Sunan Ampel untuk meningkatan softskill dan pengalaman santriwan/santriwati dalam berwirausaha melalui program pelatihan “Pembuatan Sambal Pecel Instan Di Kawasan Pondok Pesantren Raden Rahmat Sunan Ampel”. Kegiatan ini ditujukan untuk santriwan/santriwati Pondok Pesantren Raden Rahmat Sunan Ampel. Permasalahan utama yang terdapat pada mitra binaan yaitu kurangnya pemahaman mengenai proses pembuatan sambal pecel siap saji/instan, teknik pengemasan yang akan digunakan hingga strategi pemasaran yang dilakukan. Oleh karena itu kegiatan ini berfokus pada pembuatan sambal pecel siap saji hingga strategi pemasaran. Metode pelatihan terdiri dari pemaparan materi dan demo/praktek pembuatan sambal pecel. Santri dan santriwati sangat antusias dengan kegiatan pengabdian ini. Diharapkan dengan pelatihan yang diberikan, mampu membuat peserta siap berwirausaha dan lebih sejahtera.
Development of Enzyme Production Technology with Double Protease Immobilization Method (Calotropin and Papain) Witono, Yuli; Masahid, Ardiyan Dwi; Giyarto, Giyarto; Belgis, Maria; Jayanti, Puja Dwi; Wahyuni, Livia
Indonesian Food Science and Technology Journal Vol. 8 No. 2 (2025): Volume 8. Number 2, July 2025 |IFSTJ|
Publisher : Department of Technology of Agricultural product (THP) Jambi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/ifstj.v8i2.37509

Abstract

Abstract—Enzymes are biocatalysts widely applied in agriculture, food processing, chemical industries, and medical fields. Enzymes can be used singly or immobilized within a matrix. However, free enzymes often exhibit lower stability compared to immobilized enzymes. Therefore, this research aimed to evaluate the combined two proteolytic enzymes for extracting protein hydrolysates. The objective was to analyze enzymes' immobilization characteristics using carrageenan and determine the optimal concentration combination. Calotropin and papain were applied at concentrations of 2.5 mL, 3.5 mL, and 4.0 mL, respectively. The analysis parameters included yield, enzyme activity, soluble protein content, specific enzyme activity, stability, and kinetic parameters (Km and Vmax). The results showed that the combined proteases exhibited yields ranging from 44.43% to 48.63%; enzyme activities of 9.23–53.63 U/mL; soluble protein contents of 2.40–2.89 mg/mL; and specific enzyme activities of 3.54–18.62 U/mg protein. The enzymes were maintained stability at temperatures ranging from 30 to 60 °C. The kinetic parameters were determined as Km = 0.0034 g/mL and Vmax = 17.094 U/min. 
PROFIL MUTU ABON JAMUR TIRAM (PLEUROTUS OSTREATUS) HASIL PENGGUNAAN VARIASI TEKNIK PENGGORENGAN DAN JENIS MINYAK Masahid, Ardiyan Dwi; Hakim, Alfian Rizky; Fauzi, Mukhammad
Jurnal Penelitian Sains dan Teknologi Indonesia Vol 1 No 2 (2022): Jurnal Penelitian Sains dan Teknologi Indonesia (JPSTI)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpsti.v1i2.223

Abstract

Produksi jamur tiram yang semakin meningkat dan umur simpannya yang rendah, maka jamur tiram perlu dilakukan pengolahan menjadi suatu produk yaitu abon. Proses pengolahan abon dapat dipengaruhi oleh teknik penggorengan dan jenis minyak. Penelitian ini bertujuan mengetahui pengaruh teknik penggorengan dan jenis minyak terhadap sifat fisik, kimia dan kesukaan panelis. Hasil analisa berpengaruh signifikan terhadap sifat fisik dan kimia abon jamur tiram dengan perlakuan A1B1 (Deep frying dan minyak sawit); A1B2 (Deep frying dan minyak kelapa); A2B1 (Pan frying dan minyak sawit); dan A2B2 (Pan frying dan minyak kelapa) yang memilki nilai kecerahan warna berkisar 52,20-54,57; kadar air berkisar 8,10-9,36%; kadar abu berkisar 7,20-7,91%; kadar protein terlarut berkisar 2,09-1,51%; kadar lemak berkisar 6,83-11,69 %; dan serat kasar berkisar 17,59-18,60%. Perlakuan menggunakan pan frying dan minyak sawit pada abon jamur tiram merupakan perlakuan terbaik dan paling disukai oleh panelis.
PENGARUH PERBEDAAN KONSENTRASI GEL OKRA HIJAU DAN KARAGENAN TERHADAP KARAKTERISTIK JELLY DRINK OKRA HIJAU (Abelmoschus Esculentus L.) Rosyada, Amrina; Masahid, Ardiyan Dwi; Brihatsama; Priyantono, Eko
Jurnal Penelitian Sains dan Teknologi Indonesia Vol 4 No 1 (2025): Jurnal Penelitian Sains dan Teknologi Indonesia (JPSTI)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The potential of green okra lies in its high dietary fiber component. Efforts to utilize dietary fiber in green okra include processing it into jelly drink product. The aim of this is to determine the influence of adding concentrations of green okra gel and carrageenan on the physical, chemical and organoleptic properties, as well as to determine the appropriate formulation of different concentrations of green okra gel and carrageenan to produce jelly drink green okra with the best physical, chemical and organoleptic properties. The experimental design used was a factorial completely randomized design (CRD) consisting of green okra gel concentrations (A) of 15%, 30%, 45%, 60%, and carrageenan concentrations (B) of 0.30% and 0.40%. The observational data were analyzed using ANOVA followed by DMRT test. Based on the research results, it can be concluded that the differences in green okra gel concentration and carrageenan concentration significantly affect the a* (redness), b* (yellowness), total solids, syneresis, pH, color liking score, aroma, taste, texture, and overall liking. The effectiveness values indicate that the best-treated green okra jelly drink has a pH value of 3.42, syneresis of 30.12%, total solids content of 14.17%, and dietary fiber content of 0.81%. In the organoleptic test, color liking score was 6.68 (like), taste 6.0 (like), aroma 5.64 (somewhat like), texture 5.48 (somewhat like), and overall liking 6.16 (like).
SOSIALISASI MAKANAN HALAL PADA ANAK SEKOLAH DASAR DENGAN PENDEKATAN EDUKATIF DI AIS (AL-MUNAWWIRIY ISLAMIC SCHOOL) SUMBERSARI JEMBER Masahid, Ardiyan Dwi; Belgis, Maria; Witono, Yuli; Nafi', Ahmad; Azkiyah, Lailatul; Meilina, Lita; Brihatsama; Wahyuni, Livia
Jurnal Hasil Pengabdian kepada Masyarakat Universitas Jember Vol 4 No 1 (2025): Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember
Publisher : LP2M Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The majority of Indonesia's population is Muslim; therefore, halal food education should be instilled from an early age, particularly at the elementary school. The activity aimed to enhance students’ literacy and understanding of halal food through an educational approach implemented at Al-Munawwiriy Islamic School (AIS), Sumbersari, Jember. The activity was conducted by the Research Group (Keris E-Food) from University of Jember in collaboration with AIS. The program began with an introduction Elmarom Bread production process, continued with an assessment of students’ initial understanding, and concluded with interactive socialization sessions. Educational methods employed included visual media, group discussions, and direct observation of the halal food production process. The results indicated that this educational approach was highly effective in increasing students’ knowledge of the concepts of halal and thayyib, as well as in fostering a critical attitude in selecting food in accordance with Islamic principles. Furthermore, the program reinforced the role of schools as institutions for embedding Islamic values. Early halal education has proven to be a strategic effort in shaping a generation that is both aware of and committed to halal food consumption.
Physical, Chemical, Organoleptic Characteristics, and Antioxidant Activity of Instant Cascara Powder with Additions of Ginger and Sappan Wood Masahid, Ardiyan Dwi; Yulianingtyas, Resya Elen; Witono, Yuli; Nafi, Ahmad
agriTECH Vol 44, No 1 (2024)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/agritech.78340

Abstract

The aroma and taste of cascara products are generally mild, and the use of cascara powder also produces a blackish-brown extract color. To enhance both the taste and aroma, it is recommended to add ginger (Zingiber officinale) and sappan wood (Caesalpinia sappan). Ginger contains essential oils to enhance aroma and has antioxidant activity. On the the hand, the Brazilin compound in sappan wood provides a natural red color, and the polyphenols have antioxidant activity. This study aimed to determine the effects of adding ginger and sappan wood extracts on physical, chemical, and organoleptic properties, as well as the antioxidant activity of instant cascara powder, with a focus on selecting the best and most preferred formulation. A Completely Randomized Design (CRD) was used with one factor, namely concentration of ginger and sappan wood extracts. The treatment consisted of six levels, including F1 as control (cascara 100 g), F2 (cascara 70 g, ginger 5 g, and sappan wood 25 g), F3 (cascara 70 g, ginger 10 g, and sappan wood 20 g), F4 (cascara 70 g, ginger 15 g, and sappan wood 15 g), F5 (cascara 70 g, ginger 20 g, and sappan wood 10 g), and F6 (cascara 70 g, ginger 25 g, and sappan wood 5 g). The results showed that additions of ginger and sappan wood extracts impacted physical property, namely redness value, chemical properties including moisture, polyphenol content, and antioxidant activity, as well as all organoleptic parameters. The best and most preferred formulation was sample F2 which had a redness value of 6,39, moisture content of 2,62%, polyphenol content of 35,41 mg GAE/g, and antioxidant activity amounting to 64,5%. Moreover, the average value of color preference was 5,32 (somewhat like), fragrance 4,32 (neutral), taste 4,8 (neutral – somewhat like), and overall 4,84 (neutral – somewhat like).
Co-Authors Ahmad Nafi Amrina Rosyada Azkiyah, Lailatul Brihatsama Cahyaningati, Kinanti Eko Priyantono Giyarto Giyarto Hakim, Alfian Rizky Iwan Taruna Jayanti, Puja Dwi Majid, Nur Ika Okta Maria Belgis Maryanto - Meilina, Lita Mukhammad Fauzi Nafi', Ahmad Wahyuni, Livia Yuli Witono Yulianingtyas, Resya Elen