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Siampa, Jainer Pasca
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Journal : Jurnal MIPA

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Uji Antibakteri Sediaan Masker Peel Off Ekstrak Etanol Daun Alpukat (Persea ameicana Mill.) terhadap Bakteri Staphylococcus epidermidis sebagai Antijerawat Puluh, Esterlina Aldora; Edy, Hosea Jaya; Siampa, Jainer Pasca
Jurnal MIPA Vol 8, No 3 (2019)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.8.3.2019.25773

Abstract

Daun Alpukat memiliki kandungan antara lain saponin, alkaloid, flavonoid, polifenol, quersetin yang bersifat sebagai antibakteri.  Penelitian ini bertujuan untuk mendapatkan konsentrasi terbaik dari ekstrak etanol daun alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off berdasarkan uji aktivitas antibakteri terhadap bakteri Staphylococcus epidermidis. Penelitian ini menggunakan metode ekperimental laboratorium. Formula sediaan masker gel peel off ekstrak daun Alpukat dibuat dengan variasi konsentrasi 0.1%; 0.15%; 0.2%; 0.25% dan 0.3%. Ekstrak tanaman daun alpukat diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Pengujian antibakteri dengan metode sumuran diameter zona hambat yang paling besar yaitu pada konsentrasi 0,3% dengan diameter zona hambat 3 mm ± 0,32 dan kemampuan daya hambat dikategorikan lemah. Dapat disimpulkan bahwa konsentrasi terbaik dari ekstrak etanol daun Alpukat (Persea americana Mill.) yang diformulasikan sebagai sediaan masker gel peel off yaitu pada konsentrasi 0.3% dan dikategorikan kemampuan daya hambat lemahAvocado leaves contains saponins, alkaloids, flavonoids, polyphenols, quercetin which are as antibacterial properties. This study aims to obtain the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) which is formulated as a gel peel off mask preparation based on antibacterial activity test against Staphylococcus epidermidis bacteria. This study uses a laboratory experimental method. Avocado leaf extract peel off mask gel formula is made with a concentration variation of 0.1%; 0.15%; 0.2%; 0.25% and 0.3%. Avocado leaf extract is obtained by maceration using 96% ethanol solvent. Antibacterial testing with the largest inhibition zone diameter wells method is at a concentration of 0.3% with a diameter of inhibition zone 3 mm ± 0.32 and the ability of inhibition is categorized as weak. It can be concluded that the best concentration of ethanol extract of avocado leaves (Persea americana Mill.) Which is formulated as a gel peel off mask preparation is at a concentration of 0.3% and categorized as weak inhibitory ability
Uji Efektivitas Antibakteri Sediaan Krim Ekstrak Etanol Daun Kaliandra (Calliandra surinamensis) Terhadap Bakteri Staphylococcus aureus Somba, Gichella C.J; Edy, Hosea Jaya; Siampa, Jainer Pasca
Jurnal MIPA Vol 8, No 3 (2019)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.8.3.2019.25776

Abstract

Daun Kaliandra (Calliandra surinamensis) mengandung senyawa flavonoid, saponin dan tannin yang mampu menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk memformulasi dan mengetahui efektivitas antibakteri sediaan krim ekstrak etanol daun Kaliandra terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium. Formula sediaan krim dibuat dengan variasi konsentrasi ekstrak etanol daun Kaliandra 2%, 3%, 4%, 5%, 6%. Ekstrak daun Kaliandra diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Penelitian uji antibakteri sediaan krim esktrak etanol daun Kaliandra menggunakan metode sumuran pada bakteri Staphylococcus aures menghasilkan daya hambat yang terbesar 5,09±0,88 mm pada konsentrasi 6%. Dapat disimpulkan bahwa sediaan krim ekstrak etanol daun Kaliandra memiliki efektivitas  antibakteri yang sedangKaliandra leaf (Calliandra surinamensis) contains flavonoid compounds, saponins and tannins that can inhibit bacterial growth. The aim of this study was to formulate and investigate the antibacterial effectiveness of Kaliandra leaf ethanol extract cream against Staphylococcus aureus. This research uses laboratory experimental methods. Cream preparation formula is made with variations in the concentration of ethanol extract of Kaliandra leaves 2%, 3%, 4%, 5%, 6%. Kaliandra leaf extract was obtained by maceration using 96% ethanol solvent. The antibacterial test study of ethanol extract of Kaliandra leaf extract using the method of Staphylococcus aures produced the greatest inhibition of 5.09 ± 0.88 mm at a concentration of 6%. It can be concluded that the ethanol extract of Kaliandra leaf cream has moderate antibacterial effectivenessDaun Kaliandra (Calliandra surinamensis) mengandung senyawa flavonoid, saponin dan tannin yang mampu menghambat pertumbuhan bakteri. Penelitian ini bertujuan untuk memformulasi dan mengetahui efektivitas antibakteri sediaan krim ekstrak etanol daun Kaliandra terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium. Formula sediaan krim dibuat dengan variasi konsentrasi ekstrak etanol daun Kaliandra 2%, 3%, 4%, 5%, 6%. Ekstrak daun Kaliandra diperoleh dengan cara maserasi menggunakan pelarut etanol 96%. Penelitian uji antibakteri sediaan krim esktrak etanol daun Kaliandra menggunakan metode sumuran pada bakteri Staphylococcus aures menghasilkan daya hambat yang terbesar 5,09±0,88 mm pada konsentrasi 6%. Dapat disimpulkan bahwa sediaan krim ekstrak etanol daun Kaliandra memiliki efektivitas  antibakteri yang sedang
Perbandingan Profil Penetrasi Formula Krim Antioksidan dari Ekstrak Perikarpium Buah Manggis (Garcinia mangostana) dengan Variasi Penetration Enhancer Siampa, Jainer Pasca; Lebang, Julianri Sari; Antasionasti, Irma; Nurmiati, Nurmiati
Jurnal MIPA Vol 10, No 1 (2021)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.10.1.2021.31146

Abstract

Paparan sinar matahari dengan kandungan radiasi UV merupakan salah satu faktor pencetus radikal bebas penyebab penuaan dini (Photoaging), yang ditandai dengan hiperpigmentasi, kerutan, dan menurunnya elastisitas kulit. Solusi permasalahan ini adalah penggunaan antioksidan yang dapat menangkal terbentuknya radikal bebas. Tujuan dari penelitian ini adalah untuk membuat formula krim antioksidan dari ekstrak perikarpium kulit manggis dengan perbedaan penetration enhancer untuk mendapatkan persentase penetrasi dan retensi terbaik di kulit. Ekstrak perikarpium buah manggis dibuat dengan metode maserasi menggunakan etanol 70% kemudian diukur aktivitas antioksidannya dan kandungan fenoliknya, selanjutnya digunakan sebagai bahan aktif dalam formula krim. Formula F1-F3 dibuat kemudian dievaluasi karakteristik fisiknya dan dilakukan pengujian penetrasi secara in vitro. Pengujian penetrasi secara in vitro dilakukan terhadap F2 dan F3 yang mengandung ekstrak. Hasil IC50 ekstrak terhadap DPPH sebesar 7,514 µg/mL dan kandungan fenolik sebesar 112,70 ± 0,80 mgGAE/g. Nilai persentase penetrasi, persentase retensi, dan nilai flux secara berturut-turut untuk F2 sebesar 9,96% ± 0,075%; 37,951% ± 0,103%; 3,037 ± 0,023 µg/cm2.jam dan F3 sebesar 7,872% ± 0,078% ;  33,874% ± 0,259%; 2,400 ± 0,024 µg/cm2.jam. Dari hasil analisis statistik, disimpulkan bahwa F2 adalah formula terbaik dengan karakteristik fisik yang homogen, memiliki warna kekuningan, tipe emulsi m/a, pH sediaan 6, daya lekat 11,05 ± 0,87 detik, dan diameter daya sebar 6,20 ± 0,10 cm.Sun exposure with UV radiation is one of the triggers for free radicals that cause premature aging (photoaging), characterized by hyperpigmentation, wrinkles, and decreased skin elasticity. The solution to this problem is the use of antioxidants that can prevent the formation of free radicals. The purpose of this study was to formulate an antioxidant cream dosage form from pericarpium extract of mangosteen pericarpium with different penetration enhancers to obtain the best percentage of penetration and retention in the skin. Pericarpium extract of mangosteen fruit was prepared by maceration method using 70% ethanol, then the antioxidant activity and the phenolic content were measured and were used as an active ingredient in the cream formula. Formula F1-F3 were made, their physical characteristics were evaluated, and in vitro penetration testing was carried out. In vitro penetration testing was carried out on F2 and F3 containing extracts. The IC50 value of the extract against DPPH was found to be 7.514 µg/mL and the phenolic content was found to be 112,70 ± 0,80 mgGAE/g. The percentage of penetration, retention percentage, and flux value were 9.96% ± 0.075%, 37.951% ± 0.103%, 3.037 ± 0.023 µg/cm2.hour respectively for F2 and 7.872% ± 0.078% , 33.874% ± 0.259%,  2.400 ± 0.024 µg/cm2.hour, respectively for F3. From the results of statistical analysis, it was concluded that F2 was the best formula with homogeneous physical characteristics, had a yellowish color, emulsion type w/o, pH value of 6, adhesion of 11.05 ± 0.87 seconds, and diameter of spreadability of 6.20 ± 0.10 cm.
Profil Penetrasi Sediaan Gel Antioksidan Ekstrak Kayu Manis (Cinnamomum burmanii) dengan Variasi Hydrocolloid sebagai Gelling agent Siampa, Jainer Pasca; Wiyono, Weny Indayany; Lestari, Utami Sasmita; Lebang, Julianri Sari; Antasionasti, Irma
Jurnal MIPA Vol 11, No 1 (2022)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.v11i1.35787

Abstract

The exposure of sunlight consisting UV radiation has been reported to be the main cause of skin cancer and contribute about 80% of the incidence of skin damage. In an attempt to overcome this problem, antioxidant agents have been used both orally and topically. Compared to oral administration, topical route has been found to be preferred due to the direct action to the desired site. Amongst topical preparation, gel possesses numerous because it provides a cool and fresh sensation. Here, we developed gel antioxidant containing cinnamon extract as active ingredient. Cinnamon was selected as it has a very strong antioxidant activity. This study aimed to obtain the penetration profile of the antioxidant gel of cinnamon extract prepared from two different hydrocolloid agents. The gels were made using various concentrations of Carbopol 940 and NaCMC. The prepared gels were further characterized for their antioxidant capacities using the DPPH free radical scavenging method and their total phenolic contents using the spectrophotometric method. The formulation possessing the strongest antioxidant capacity was continued for ex vivo penetration study using Franz diffusion cells. The results exhibited that the total phenolic value of the cinnamon extract obtained was 908.38 ± 6.54 mg GAE/g extract. The penetration percentages for the formula with Carbopol and NaCMC bases were 53.310 ± 1.217% and 27.969 ± 1.151%, respectively, and the retention percentages were 28.495 ± 0.031% and 18.356 ± 0.191%, respectively. Based on these findings, we concluded that cinnamon extract could potentially be formulated into antioxidant gel preparations and formulas based on Carbopol showed the best penetration profile.
Efek Antibakteri Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) dan Sediaan Masker Gel ¬Peel-Off Ekstrak Etanol Daun Kelor (Moringa oleifera Lam.) Tunas, Theresia H.; Edy, Hosea Jaya; Siampa, Jainer Pasca
Jurnal MIPA Vol 8, No 3 (2019)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jmuo.8.3.2019.25778

Abstract

Daun Kelor (Moringa oleifera Lam.) bermanfaat untuk menghambat aktivitas pertumbuhan bakteri karena adanya kandungan senyawa flavonoid , alkaloid, dan fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari ekstrak etanol daun Kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor terhadap bakteri Staphylococcus aureus. Penelitian ini menggunakan metode eksperimental laboratorium, ekstrak etanol daun kelor dan sediaan masker gel peel-off ekstrak etanol daun Kelor dibuat dengan konsentrasi 1, 3, 5, 7, dan 9%. Ekstrak daun Kelor diperoleh dengan cara maserasi dengan menggunakan pelarut etanol 96%. Pada pengujian antibakteri menggunakan metode sumuran. Diameter zona hambat paling besar yang ditimbulkan oleh ekstrak etanol daun Kelor yaitu pada konsentrasi 7% dengan diameter zona hambat sebesar 5,75 mm, sedangkan pada sediaan masker gel peel-off tidak menunjukkan adanya daya hambat. Dapat disimpulkan bahwa ekstrak etanol daun Kelor memiliki aktivitas antibakteri yang termasuk dalam kategori sedang.Moringa oleifera Lam. (Moringa oleifera) leaves are useful for inhibiting bacterial growth activity due to the presence of flavonoid, alkaloid, and phenol compounds. This study aims to test the antibacterial activity of the Moringa leaf ethanol extract and the gel peel-off mask preparation of Moringa leaf ethanol extract against Staphylococcus aureus bacteria. This study uses laboratory experimental methods, Moringa leaf ethanol extract and gel mask peel-off preparation for Moringa leaf ethanol extract made with concentrations of 1, 3, 5, 7, and 9%. Moringa leaf extract was obtained by maceration using 96% ethanol solvent. In antibacterial testing using the well method. The biggest inhibition zone diameter caused by ethanol extract of Moringa leaves is at a concentration of 7% with a inhibition zone diameter of 5.75 mm, whereas the peel-off gel mask preparation did not show any inhibitory power. It can be concluded that the ethanol extract of Moringa leaves has antibacterial activity which is included in the medium category.
Co-Authors Abdullah, Surya Sumantri Adelfia Papu Adithya Yudistira Antasionasti, Irma Anton, Nurhati Asep Rahman Datu, Olvie Syenni Defny Silvia Wewengkang Desinta, Ni Wayan Dian Pratiwi Edi, Hosea Jaya Edwin de Queljoe Fatimawali . Gayatri Citraningtyas Hosea J. Edy, Hosea J. Hosea Jaya Edy Jayanto, Imam Julianri Sari Lebang, Julianri Sari Kalesaran, Angela Fitriani Clementine Karlah L. R. Mansauda Khairuddin Lahamendu, Beatriks Lestari, Utami Sasmita Masengi, Kyoko Itsuko Etsuko Gabriela Meilani Jayanti Nelwan, Sabine A. Nurbayasanti, Ni Luh Nurmiati Nurmiati Paputungan, Wazhifa Andarini Paulina yamlean Puluh, Esterlina A. Puluh, Esterlina Aldora Purnomo, Novia Hanna Purukan, Christy Rajab, Mustika Nur Ritan, Yosefa E.H. Rorrong, Arnelita Gloryensha Rumayar, Ricky C. Rumoroy, Jhon D. Sahi, Makbul Renaldi Sandala, Ecleysia Retika Frolen Sekeon, Crista Gretasia Seru, Eufrasia R. Shintia, Arini Somba, Gichella C. J. Somba, Gichella C.J Sri Sudewi, Sri Sukartiningsih, Yulita Ni Nyoman Sukartiningsih, Yulita Ni Nyoman Tri Tamimi, Alyah A.P Ticoalu, Pricilia Kesia TRINA EKAWATI TALLEI Tulung, Grace Laury Tumilaar, Sefren Geiner Tunas, Theresia H. Widdhi Bodhi Widiastuti, Priskha Widya Astuty Lolo, Widya Astuty Wiyono, Weny Indayany