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All Journal Berkala Ilmiah Pertanian JURNAL ILMU PERTANIAN Pelita Perkebunan Agriprima : Journal of Applied Agricultural Sciences Jurnal Agrotek Tropika Jurnal Ilmiah Hijau Cendekia Kumawula: Jurnal Pengabdian Kepada Masyarakat Unram Journal of Community Service (UJCS) PAPUMA: Journal of Comunnity Services
Munandar, Denna Eriani
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences

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Journal : Jurnal Agrotek Tropika

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THE EFFECT OF TDZ (Thidiazuron) ON THE FORMATION OF DIRECT SOMATIC EMBRYOGENESIS IN Phalaenopsis sp. ORCHID Adinda, Intan Dwi; Restanto, Didik Pudji; Dewanti, Parawita; Munandar, Denna Eriani; Hartatik, Sri; Prayoga, Mohammad Candra; Solikhah, Ummi
Jurnal Agrotek Tropika Vol. 13 No. 3 (2025): JURNAL AGROTEK TROPIKA VOL 13, AGUSTUS 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i3.9795

Abstract

Orchids (Phalaenopsis sp.) are among the most valuable ornamental plants due to their wide range of flower colors and high commercial demand. However, conventional propagation methods are often inefficient for large-scale production, necessitating the optimization of tissue culture techniques for rapid and uniform clonal propagation. The use of appropriate plant growth regulators, particularly thidiazuron (TDZ), is crucial for enhancing somatic embryogenesis—a key pathway for orchid micropropagation. This study aimed to determine the optimal concentration of TDZ for inducing somatic embryogenesis in Phalaenopsis sp. through histological analysis and scanning electron microscopy (SEM) observations. A completely randomized design was employed using Murashige and Skoog (MS) medium supplemented with four TDZ concentrations (1, 3, 5, and 7 mg/L). Leaf explants of Phalaenopsis sp. served as the explant source. The results demonstrated that direct somatic embryogenesis successfully occurred from leaf explants across treatments. Among the tested concentrations, 5 mg/L TDZ produced the most effective response, resulting in the highest somatic embryo formation rate (32%) and the shortest time to embryo maturation (37 days after culture initiation). The embryos exhibited characteristic dark green coloration and a crumbly texture. These findings highlight the pivotal role of TDZ in promoting somatic embryogenesis in Phalaenopsis sp., providing a reliable protocol for efficient orchid propagation. The study contributes to the advancement of orchid biotechnology by offering histological and ultrastructural evidence that supports the optimization of clonal propagation systems for commercial and conservation purposes.
Co-Authors . Usmadi Adinda, Intan Dwi Agung Sih Kurnianto Aritonang, Firman Arum, Ayu Puspita Azmi Saleh Dyah Ayu Savitri Ebrilyani, Elok Farisi, Oria Alit Hadi Suwono Harsanti, Restiani Sih Hidayatullah, Raihan Ika Purnamasari Kanzana, Gebina Khozin, Mohammad Nur Kusmanadhi, Bambang Maharani, Aulia Febrina Maulidi, Fathoni Ilham Musyaffa', Hudzaifah Pakpahan, Tioma Stephani Parawita Dewanti Patricia SM, Susan Barbara Pramudya, Frisco Sendy Pratiwi, Helti Anggiana Prayoga, Mohammad Candra Restanto, Didik Pudji Rifeni, Meyga Ridi Rofikin, Mochammad Zaini Sigit Soeparjono Slameto Slameto Sri Hartatik Sundahri, Sundahri Suud, Hasbi Mubarak TRI HANDOYO Ummi Solikhah, Ummi Wati, Henny Diana