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All Journal Indonesian Journal of Cancer Chemoprevention Journal of Agromedicine and Medical Sciences Pharmacon JSFK (Jurnal Sains Farmasi & Klinis) JPSCR : Journal of Pharmaceutical Science and Clinical Research Abdimasku : Jurnal Pengabdian Masyarakat Journal of Pharmaceutical Care Anwar Medika Journal of Pharmaceutical and Sciences. Journal of Agropharmacy Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice)
Dianasari, Dewi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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Antiproliferative activity of Ethanolic Extract of Kembang Bulan (Tithonia diversifolia) Leaf on HeLa Cervical Cancer Cell Line Puspitasari, Endah; Nuri, Nuri; Ningsih, Indah Yulia; Triatmoko, Bawon; Dianasari, Dewi
Indonesian Journal of Cancer Chemoprevention Vol 13, No 1 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss1pp55-60

Abstract

Tithonia diversifolia has been showed to be cytotoxic and antiproliferative on colon cancer, glioblastoma, hepatoma, kidney cancer, breast cancer, lung cancer, melanoma, leukemia, ovary cancer, prostate cancer, and stomach cancer cell lines, but not on cervical cancer cells yet. Our research aimed to determine the cytotoxicity and antiproliferative activity of T.diversifolia leaf ethanolic extract on HeLa cervical cancer cell line. The cytotoxicity and the antiproferative activity assay were done using MTT method for 24 h for cytotoxic assay; and series of 24, 48, and 72 h for antiproliferative assay. The cytotoxic activity was analyzed using IC50, while the antiproliferative assay was analyzed based on the proliferation kinetics. All assays were done in triplicate. T.diversifolia leaf ethanolic extract exhibited strong cytotoxic activity on HeLa cervical cancer cell lines with the IC50 of 97.839±10.120 μg/mL. The cytotoxic activity was dose dependent. Based on the proliferation assay, the antiproliferative activity was stronger as the incubation time and the dose increases. T.diversifolia leaf ethanolic extract showed strong cytotoxic and antiproliferative activity on HeLa cervical cancer cell lines.Keywords: T.diversifolia leaf ethanolic extract, cytotoxicity assay, antiproliferative assay, HeLa cervical cancer cells.
The Relationship of In Vitro Anti-Inflammatory and Antioxidant Activity with Total Phenolic and Flavonoids Content of Tithonia diversifolia Leaves Ethanolic Extract and Fractions Puspitasari, Endah; Nuri, Nuri; Muslichah, Siti; Triatmoko, Bawon; Dianasari, Dewi
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 9, No 2 (2024)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v9i2.83109

Abstract

A previous study showed that Tithonia diversifolia ethanolic extract has antiproliferative properties against HeLa cervical cancer cells. It has also shown anti-inflammatory properties in vivo, one of the approaches to cancer chemoprevention. This research aimed to determine the relation of in vitro anti-inflammatory and antioxidant activity of T. diversifolia leaves ethanolic extract and fractions with total phenolic and flavonoid content. The anti-inflammatory was evaluated in vitro using red blood cell (RBC) membrane stabilization and inhibition of protein denaturation method, while antioxidant activity was done using the DPPH method. Phytochemical screening was done using the TLC method. Total phenolic and flavonoid content were also counted using the colorimetry method. ANOVA followed by LSD post hoc, multiple regression analysis, and Pearson correlation were used to analyze the data. Compared to its fractions, the highest anti-inflammatory and antioxidant activity was obtained from T. diversifolia leaves ethanolic extract. The T. diversifolia leaves ethanolic extract contained terpenoids, alkaloids, polyphenols, and flavonoids. Overall, the antioxidant property contributed more to RBC membrane stabilization than protein denaturation inhibition. The phenolic and flavonoid content contributed most to antioxidant and red blood cell membrane stabilization rather than protein denaturation inhibition.
Co-Authors Arif Rahman Hakim Bawon Triatmoko Endah Puspitasari Evi Umayah Ulfa Fifteen Aprila Fajrin Firdiyansari, Irawati Iftitah, Maulidya Barikatul Indah Yulia Ningsih Indah Yulia Ningsih Mochammad Amrun Hidayat Nasititi, Fauzia Ken Nidya Meilani Nuri Nuri Pratama P., M. Reynaldi Archan Tanfidz Alishlah, Tanfidz