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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Sains Dasar Jurnal Teknosains Journal of Degraded and Mining Lands Management Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengolahan Pangan Eksakta : Berkala Ilmiah Bidang MIPA INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Narra J
Retnaningrum, Endah
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary

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KEANEKARAGAMAN BAKTERI PADA RUMPUT LAUT (Kappaphicus alvarezii) DI PERAIRAN PANTAI AMAL Ihsan, Burhanuddin; Yulma, Yulma; Retnaningrum, Endah
Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Vol 18, No 4 (2022): SAINTEK PERIKANAN
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijfst.18.4.229-233

Abstract

Rumput laut (Kappaphycus alvarezii) merupakan komoditas penting perikanan yang memiliki nilai ekonomi cukup tinggi dan menjadi salah satu sumber devisa negara serta sumber pendapatan bagi masyarakat pesisir. Selain itu rumput laut banyak digunakan sebagai bahan dasar industri makanan, farmasi dan energi. Namun produksi rumput laut kota Tarakan pada tahun 2019 mengalami penurunan dari 159.468 ton menjadi 152.76 ton. Salah satu yang menyebabkan terjadinya penurunan produksi rumput laut adalah serangan penyakit  ice-ice yang disebabkan oleh bakteri. Tujuan dari penelitian untuk mengetahui keanekaragaman bakteri yang terdapat pada rumput laut di perairan Pantai Amal Tarakan. Isolasi bakteri dilakukan dengan menggunakan metode stread plat pada media TCBS (thiosulphate citrate bile salt sucrose) dan TSA (thiosulphate sucrosa agar). Sampel rumput laut dihaluskan lalu diambil sebanyak 5 gram kemudian dimasukkan ke dalam cawan petri yang berisi media TCBS dan TSA. Selanjutnya diinkubasi pada suhu 370C atau suhu ruangan selama 24 jam. Identifikasi bakteri dilakukan dengan mengacu pada buku Bergey’s Manual of Determinatif Bacteriology dan Manual For the Identification of Medical Bacteria dengan melakukan uji biokimia yang meliputi; Uji Pewarnaan Gram, Test Oksidase, Katalase, O/F (Oksidasi/Fermentasi), glukosa, motility, Produksi asam dari karbohidrat (D-Glukosa dan D-mannitol), lysin, urea, ornithin dan methil red. Hasil penelitian menunjukkan bahwa terdapat Keanekaragaman bakteri pada rumput laut (Kappaphycus alvarezii) di perairan Pantai Amal Tarakan diantaranya Corynebacterium, Acinetobacter, Bacillus, Pseudomonas dan Vibrio yang berpotensi menyebabkan penyakit ice-ice. Rekomendasi: Perlu menjaga keseimbangan lingkungan dengan memperhatikan parameter kualitas air, agar rumput laut tidak stres, sebab infeksi bakteri bersifat sekunder. Seaweed (Kappaphycus alvarezii) is an important fishery commodities that has a high economic value and is a source of foreign exchange and a source of income for coastal communities. In addition, seaweed is widely used as a basic material for the food, pharmaceutical and energy industries. However, Tarakan's seaweed production in 2019 decreased from 159.468 tons to 152.576 tons. One of the causes of a decrease in seaweed production is the attack of ice-ice disease caused by bacteria. The purpose of this study was to determine the diversity of bacteria found in seaweed in the waters of Amal Beach, Tarakan. Bacterial isolation was carried out using TCBS (thiosulphate citrate bile salt sucrosa) and TSA (thiosulphate sucrosa agar) media. Seaweed samples were mashed and then taken as much as 5 grams and then put into a petri dish containing TCBS and TSA media. Then incubated at 37°C or room temperature for 24 hours. Identification of bacteria is carried out by referring to the book Bergey's Manual of Determinative Bacteriology and Manual For the Identification of Medical Bacteria by conducting biochemical  tests which include; Gram stain test, oxidase test, catalase, O/F (Oxidation-Fermentation), glucose, motility, acid production from carbohydrates (D-glucose and D-mannitol), lysine, urea, ornithine and methyl red. The results showed that there was a diversity of bacteria in seaweed (Kappaphycus alvarezii) in the waters of the Tarakan Amal Coast including Corynebacterium, Acinetobacter, Bacillus, Pseudomonas and Vibrio which have the potential to cause ice-ice disease. Recommendation: It is necessary to maintain environmental balance by paying attention to water quality parameters, so that seaweed is not stressed, because bacterial infections are secondary.
Isolation of Cellulolytic Microbes from Bio-Slurry Muammar, Arief; Manullang, Maria; Arjuna, Mesha; Retnaningrum, Endah
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 1 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.564 KB) | DOI: 10.24036/eksakta/vol22-iss1/257

Abstract

Cellulolytic microbes produce cellulase enzymes that can degrade cellulose. Cellulolytic microbes are found in many habitats, including in cattle waste (bio-slurry), which is result of hydrolysis of cattle feed ingredients sourced from cellulose. Cellulase enzymes play a role in the process of hydrolyzing cellulose into glucose. This study aims to isolate cellulolytic microbes from bio-slurry and measure activity of cellulase enzymes in cellulolytic microbes isolated from bio-slurry. We are taking bio-slurry samples with 5 sample points and isolating the microbes in Carboxyl Methyl Cellulose (CMC) medium, cellulase enzyme activity qualitative test with Congo Red Assay, and perform quantitative test with the DNS Assay. Microbial isolates which shows positive results for cellulolytic activity in the qualitative test called SIIC1, SIIIC5, SIIC3. In the quantitative test can be seen based on Optical Density (OD) for cell lysate, SIIC1 0.298; SIIC3 0.186; SIIIC5 0.247; and a 0.332 for blank solution. Whereas supernatant obtain SIIC1 value of 0.237; SIIC3 0.212; SIIIC5 0.198; and 0.195 for blank solution. Based on the results, it can be concluded that the cellulolytic activity of microbes in bio-slurry based on OD values shows good results in supernatant, but it’s not significant enough in the cell lysate.
Production and characterization of lactic acid bacteria biofilms synthesized using tofu wastewater Atmandaru, Susmaya; Solichah, Amalia; Retnaningrum, Endah
Jurnal Teknosains Vol 14, No 2 (2025): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.103083

Abstract

Biofilms serve to protect microbes from environmental conditions. Biofilms produced by lactic acid bacteria (LAB) can even inhibit the growth of pathogens. Medium de Man Ragosa Sharpe (MRS) is a specific medium for LAB growth and biofilm formation; however, it is not effective on an industrial scale due to its high cost. Tofu wastewater serves as an alternative medium because it contains complete nutrients that support the formation of LAB biofilms. This study aimed to determine the effect of C and N formulation in tofu wastewater on the production and characterisation of biofilms produced by four Lactobacillus (LAB) strains, including Enterococcus casseliflavus F4IS5, E. casseliflavus F14IS5, and E. casseliflavus F14IS6. Glucose and ammonium sulfate were added to the tofu wastewater as carbon and nitrogen sources, respectively. The biofilm-forming ability of LAB was tested by the biofilm assay method. The LAB biofilm characteristics were tested based on adhesion, while the exopolysaccharide concentration, a component of the biofilm, was analysed using the dry weight method. The inhibitory activity of LAB biofilms against the growth of pathogenic bacteria, specifically Escherichia coli and Staphylococcus aureus, was tested using the microplate method. The highest LAB biofilm production was obtained from the E. casseliflavus F6IS4 isolate in a tofu wastewater medium supplemented with 2% glucose and 1% ammonium sulfate, with an incubation time of 48 hours. The biofilm produced was categorised as a strong biofilm, which also exhibited strong adhesion; the separate cells accounted for only 19.25%. Besides, the EPS production by the strain was 63.4%. The biofilm of E. casseliflavus F6IS4 in tofu wastewater, supplemented with 2% glucose and 1% ammonium sulfate, also exhibited the highest inhibitory activity against E. coli and S. aureus, at 2.7% and 2.1%, respectively.
Production and Characterization of Biosurfactants Produced by Pseudomonas aeruginosa B031 Isolated From a Hydrocarbon Phytoremediation Field Retnaningrum, Endah; Wilopo, Wahyu
BIOTROPIA Vol. 25 No. 2 (2018): BIOTROPIA Vol. 25 No. 2 August 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1295.697 KB) | DOI: 10.11598/btb.2018.25.2.808

Abstract

The biosurfactants are used by several industrial sectors such as petroleum, agriculture, food production, chemistry, cosmetics, and pharmaceuticals. Because of their hydrophobic and hydrophilic moieties, they have potency to reduce surface tension, interfacial tension between water-hydrocarbon systems, and low micelle concentration. Their characteristics strongly depend on the producer strain as well as on the medium composition, such as carbon and nitrogen sources. This study was conducted to investigate the influence of different sources of carbon (n-hexadecane, glycerol and glucose) and nitrogen (urea, NH4Cl and NaNO3) for the production of biosurfactants by a new strain of Pseudomonas aeruginosa B031 isolated from a rhizosphere of Paraserianthes falcataria L. Nielsen, a hardwood plant species at a phytoremediation field. The biosurfactant characteristics of the strain were evaluated, particularly its surface-active properties and potential to remove hydrocarbon. Glycerol was found to be the optimum carbon source, with rhamnose concentration, emulsification index, and critical micelle concentration (CMC) of 718 mg/L, 37%, and 35 mN/m, respectively. NaNO3 was observed as the optimum nitrogen source, with rhamnose concentration, emulsification index, and CMC of 290 mg/L, 30%, and 24 mN/m, respectively. These biosurfactants efficiently reduced surface tension of culture broth from 42 mN/m to 31 mN/m for the glycerol treatment and from 37 mN/m to 24 mN/m for the sodium nitrate treatment. The crude biosurfactants from the glycerol and sodium nitrate treatments also removed 87.5% and 84%, respectively, of crude oil from sand. These rates were higher than those of the chemical surfactants (SDS and Triton X-100). These findings indicate that the biosurfactants produced by the strain from both glycerol and NaNO3 treatments can efficiently decrease the interfacial tension of culture broth dilution and have a high emulsion index, thus hold promise in hydrocarbon bioremediation application.
Co-Authors Amantsura, Daimeera Anja Lulu Ardhelia Christy Anggraini Arjuna, Mesha Atmandaru, Susmaya BUDI SETIADI DARYONO Burhanuddin Ihsan, Burhanuddin Chika Giyatno, Divya Daris, Pieter Jhon Joshua Daryono, Budi S. Fatiqin, Awalul Hastuti, Luthfiana Indah Khairunisa, Mutia Asri Manullang, Maria Muammar, Arief Nurseha, Tito Prastiyanto, Muhammad E. Prastiyanto, Muhammad Evy Serang, Yitro Soleha, Siti Solichah, Amalia Sri Darmawati Syarifah Syarifah, Syarifah Udasmara, Sekarani Arifah Wahyu Wilopo Warmada, Wayan Yulma, Yulma