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All Journal HAYATI Journal of Biosciences Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Jurnal Sains Dasar Jurnal Teknosains Journal of Degraded and Mining Lands Management Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Bioeksperimen: Jurnal Penelitian Biologi Jurnal Pengolahan Pangan Eksakta : Berkala Ilmiah Bidang MIPA Narra J
Retnaningrum, Endah
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Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Public Health

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Isolation of Cellulolytic Microbes from Bio-Slurry Muammar, Arief; Manullang, Maria; Arjuna, Mesha; Retnaningrum, Endah
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 1 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (746.564 KB) | DOI: 10.24036/eksakta/vol22-iss1/257

Abstract

Cellulolytic microbes produce cellulase enzymes that can degrade cellulose. Cellulolytic microbes are found in many habitats, including in cattle waste (bio-slurry), which is result of hydrolysis of cattle feed ingredients sourced from cellulose. Cellulase enzymes play a role in the process of hydrolyzing cellulose into glucose. This study aims to isolate cellulolytic microbes from bio-slurry and measure activity of cellulase enzymes in cellulolytic microbes isolated from bio-slurry. We are taking bio-slurry samples with 5 sample points and isolating the microbes in Carboxyl Methyl Cellulose (CMC) medium, cellulase enzyme activity qualitative test with Congo Red Assay, and perform quantitative test with the DNS Assay. Microbial isolates which shows positive results for cellulolytic activity in the qualitative test called SIIC1, SIIIC5, SIIC3. In the quantitative test can be seen based on Optical Density (OD) for cell lysate, SIIC1 0.298; SIIC3 0.186; SIIIC5 0.247; and a 0.332 for blank solution. Whereas supernatant obtain SIIC1 value of 0.237; SIIC3 0.212; SIIIC5 0.198; and 0.195 for blank solution. Based on the results, it can be concluded that the cellulolytic activity of microbes in bio-slurry based on OD values shows good results in supernatant, but it’s not significant enough in the cell lysate.
Production and characterization of lactic acid bacteria biofilms synthesized using tofu wastewater Atmandaru, Susmaya; Solichah, Amalia; Retnaningrum, Endah
Jurnal Teknosains Vol 14, No 2 (2025): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.103083

Abstract

Biofilms serve to protect microbes from environmental conditions. Biofilms produced by lactic acid bacteria (LAB) can even inhibit the growth of pathogens. Medium de Man Ragosa Sharpe (MRS) is a specific medium for LAB growth and biofilm formation; however, it is not effective on an industrial scale due to its high cost. Tofu wastewater serves as an alternative medium because it contains complete nutrients that support the formation of LAB biofilms. This study aimed to determine the effect of C and N formulation in tofu wastewater on the production and characterisation of biofilms produced by four Lactobacillus (LAB) strains, including Enterococcus casseliflavus F4IS5, E. casseliflavus F14IS5, and E. casseliflavus F14IS6. Glucose and ammonium sulfate were added to the tofu wastewater as carbon and nitrogen sources, respectively. The biofilm-forming ability of LAB was tested by the biofilm assay method. The LAB biofilm characteristics were tested based on adhesion, while the exopolysaccharide concentration, a component of the biofilm, was analysed using the dry weight method. The inhibitory activity of LAB biofilms against the growth of pathogenic bacteria, specifically Escherichia coli and Staphylococcus aureus, was tested using the microplate method. The highest LAB biofilm production was obtained from the E. casseliflavus F6IS4 isolate in a tofu wastewater medium supplemented with 2% glucose and 1% ammonium sulfate, with an incubation time of 48 hours. The biofilm produced was categorised as a strong biofilm, which also exhibited strong adhesion; the separate cells accounted for only 19.25%. Besides, the EPS production by the strain was 63.4%. The biofilm of E. casseliflavus F6IS4 in tofu wastewater, supplemented with 2% glucose and 1% ammonium sulfate, also exhibited the highest inhibitory activity against E. coli and S. aureus, at 2.7% and 2.1%, respectively.
Cellulolytic Bacteria Isolation from Sugarcane Garden Soil Muammar, Arief; Arjuna, Mesha; Manullang, Maria; Retnaningrum, Endah
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 27 No. 01 (2026): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol27-iss01/632

Abstract

The soil surrounding the sugar cane garden is a promising habitat for cellulolytic bacteria because it contains a source of cellulose, hemicellulose, and other carbon sources derived from the sugar canes remaining organic matter.  This study attempts to determine the presence of cellulolytic bacteria isolated from sugar cane garden soil, as well as the ability of cellulolytic bacteria to degrade cellulose based on optical density and sugar reduction values isolated from sugarcane garden soil.  We collected soil samples from five points (T1, T2, T3, T4, T5), isolating the bacteria in CMC (Carboxyl Methyl Cellulose) medium, qualitatively testing the cellulase enzyme activity with Congo Red and Iodine Assays, and quantitatively using the DNS Assay. The results of the qualitative test with the Congo Red assay revealed that there were three positive isolates with a clearly visible clear zone, namely TII C4, TII C1, and TIV C4, and after quantitative supernatant tests, the OD values of all isolates were higher than the negative control in supernatant samples using 540nm wave length using spectrophotometer, by using synthetic cellulose called Carboxy Methyl cellulase (CMC) as a substrate in the cellulase enzyme test.
Whole-genome sequencing of endophytic Bacillus cereus ELSZ6 isolated from Smilax zeylanica L. reveals antimicrobial potential against multidrug-resistant pathogens Zuhri, Rozana; Retnaningrum, Endah; Widiyastuti, Yuli; Susidarti, Ratna Asmah
Indonesian Journal of Biotechnology Vol 31, No 2 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.116786

Abstract

The increasing global prevalence of multidrug-resistant (MDR) bacterial infections has intensified the search for new antibacterial agents from underexplored natural sources. Endophytic bacteria associated with medicinal plants are recognized as potential producers of bioactive secondary metabolites. This study aims to isolate and characterize antibacterial endophytic bacteria from Smilax zeylanica collected in Bukit Duabelas National Park, Jambi, Indonesia. Endophytic bacteria were isolated from roots, stems, leaves and rhizomes using a surface sterilization method, which was validated by a final rinse test which showed no microbial growth. Antibacterial activity was screened using a dual culture assay on Nutrient Agar against methicillin-resistant Staphylococcus aureus (MRSA) WS 29 and Escherichia coli RES 22-2020, followed by phenotypic and genomic characterization. A total of 45 endophytic bacterial strains were obtained, of which seven exhibited inhibitory activity against both pathogens. Strain ELSZ6 showed the strongest inhibition and was characterized as Gram-positive, rod-shaped, and endospore-forming. Whole genome sequencing identified the strain as Bacillus cereus (6.3 Mbp; 50× coverage). Genome mining using antiSMASH revealed several biosynthetic gene clusters associated with secondary metabolites, including bacillibactin, zwittermicin A and petrobactin. The findings indicate that strain ELSZ6 represents a potential candidate for further investigation as a source of antibacterial compounds for use against MDR bacteria.
Co-Authors Amantsura, Daimeera Anja Lulu Ardhelia Christy Anggraini Arjuna, Mesha Atmandaru, Susmaya BUDI SETIADI DARYONO Burhanuddin Ihsan, Burhanuddin Chika Giyatno, Divya Daris, Pieter Jhon Joshua Daryono, Budi S. Hastuti, Luthfiana Indah I Wayan Warmada Khairunisa, Mutia Asri Manullang, Maria Muammar, Arief Prastiyanto, Muhammad E. Prastiyanto, Muhammad Evy Ratna Asmah Susidarti Rozana Zuhri Solichah, Amalia Sri Darmawati Udasmara, Sekarani Arifah Wahyu Wilopo Yuli Widiyastuti Yulma, Yulma