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Hattu, Nikmans
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Effect Of Tamarind (Tamarindus indica L.) Extract To Histamine Content In Bullet Tuna (Auxis rochei) Hattu, Nikmans; Latupeirissa, Jolantje; Fransina, Eirene G.; Seumahu, Cecelia A; Latupeirissa, Astrid
Indonesian Journal of Chemical Research Vol 2 No 1 (2014): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2014.2-nik

Abstract

The research to determine histamine content in bullet of tuna (Auxis rochei) with variation of tamarind (Tamarindus indica L.) extract concentrations and time of marinate has been done. The histamine content is determined quantitatively by using ultraviolet-visible spectrophotometer with the calibration curve method. The result showed that histamine content in bullet tuna marinated in tamarind extract with varied concentrations of 5%, 10%, 15%, and 20% within 10 minutes, are 37.4561, 35.3041, 23.8778, and 22.1186 mg/100 g respectively. While, the concentrations of histamine in bullet tuna marinated in 20% of tamarind extract with variation of 10, 20, 30, 40, and 50 minutes, are 22.2965, 19.7544, 17.6925, 11.5865, and 8.0396 mg/100 g respectively. A qualitatively, visual comparison of colour intensity of samples with reference colour scale of the standard solution concentration can be used to determine concentrations of histamine without the aid of a spectrophotometer.
Histamine Content In Processed Bullet Tuna (Auxis thazard) Stew With Various Concentration Of NaCl Hattu, Nikmans; Telussa, Ivonne; Fransina, Eirene G.; Seumahu, Cecelia A.
Indonesian Journal of Chemical Research Vol 2 No 1 (2014): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2014.2-hat

Abstract

Analysis of histamine content in processed bullet tuna (Auxis thazard) stew flesh can be carried out by Spectrophotometry method, using p- phenildiazonium sulfonate reagent. A qualitative, visual comparison of colour intensity of samples with reference colour scale of the standard solution concentration can be used to determine levels of histamine without the aid of a spectrophotometer. The histamine content is determined quantitatively by the standard curve regression equation (y= 0.003x–0.062) with determination coefficient value (R2= 0.938). The results showed that histamine content inprocessed bullet tuna (Auxis thazard) obtained: 46.9964; 40.3093; 34.4348; 28.9900 and 24.8862 mg/100g fish, at 0.5; 1.0; 1.5; 2.0 and 2.5% concentration of NaCl, respectively.
Effect of Tamarind (Tamarindus indica L.) Extract To Histamine Content In Bullet Tuna (Auxis rochei) Hattu, Nikmans; Latupeirissa, Jolantje; Latupeirissa, Astrid
Indonesian Journal of Chemical Research Vol 2 No 2 (2015): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2015.2-nik

Abstract

The research to determine histamine content in bullet of tuna (Auxis rochei) with variation of tamarind (Tamarindus indica L.) extract concentrations and time of marinate has been done. The histamine content is determined quantitatively by using ultraviolet-visible spectrophotometer with the calibration curve method. The result showed that histamine content in bullet tuna marinated in tamarind extract with varied concentrations of 5%, 10%, 15%, and 20% within 10 minutes, are 37.4561, 35.3041, 23.8778, and 22.1186 mg/100 g respectively. While, the concentrations of histamine in bullet tuna marinated in 20% of tamarind extract with variation of 10, 20, 30, 40, and 50 minutes, are 22.2965, 19.7544, 17.6925, 11.5865, and 8.0396 mg/100 g respectively. A qualitatively, visual comparison of colour intensity of samples with reference colour scale of the standard solution concentration can be used to determine concentrations of histamine without the aid of a spectrophotometer.
Pengaruh Asam Cuka Terhadap Kandungan Histamin Dalam Daging Ikan Komu (Auxis rochei) Hattu, Nikmans; Fransina, Eirene G.; Seumahu, Cecelia A.; Sopacua, Josina
Indonesian Journal of Chemical Research Vol 3 No 2 (2016): Edisi Bulan Januari (Edition For January)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2016.3-nik

Abstract

The research on the effect of vinegar to histamine content in bullet tuna (Auxis rochei) has been done. Histamine content of quantitatively determined using a standard curve regression equation (y = 0.005 x - 0.046) with a price coefficient of determination (R2 = 0.926) is close to unity. The results showed levels of histamine in bullet tuna marinated in vinegar with variation concentrations of 5%, 10%, 15%, 20%, and 25% within 10 minutes, are 25.9099; 20.0408; 18.9671; 18.7108 and 18.6336 mg/100g respectively. While the levels of histamine in bullet tuna marinated in 25% of vinegar with variation of 10 minutes, 20 minutes, and 30 minutes, are 18.6336; 16.0550; and 15.5246 mg/100g respectively.
Uji Aktivitas Antioksidan Ekstrak Metanol Biji Kesumba Keling (Bixa orellana L) Souhoka, Fensia Analda; Hattu, Nikmans; Huliselan, Marsye
Indonesian Journal of Chemical Research Vol 7 No 1 (2019): Edisi Bulan Juli (Edition For July)
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2019.7-fas

Abstract

Kesumba keling (Bixa orellana L) has been widely used as a natural dye on lips, hair, and cloth. The red pigment in kesumba keling seeds comes from a bixin and norbixin compound which have many conjugated double bonds, so it has the potential of antioxidants. This study aims to determine the antioxidant compound and an antioxidant activity of methanol extract of kesumba keling seeds. The moisture content of kesumba keling seeds is 78.74%. The powder of kesumba keling seeds was extracted using maceration method with 80% of methanol. Phytochemical test results of methanol extract positively contained flavonoid compound. An antioxidant activity test of methanol extract of kesumba keling seeds was carried out by determining DPPH free radical deterrent activity. The absorbance measurement were made using a UV-Vis spectrophotometer at a wavelength of 517 nm. Kesumba keling seeds extract has antioxidant activity which is indicated by IC50 value of 69.425 ppm, so it is classified as a strong antioxidant.
Morphological Observation, Identification and Isolation of Tropical Marine Microalgae from Ambon Bay, Maluku Telussa, Ivonne; Hattu, Nikmans; Sahalessy, Arielno
Indonesian Journal of Chemical Research Vol 9 No 3 (2022): Edition for January 2022
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2022.9-ivo

Abstract

Microalgae are photosynthetic microorganisms that are widely distributed in waters. They have the potential as an alternative source of pigments, lipids, proteins, and carbohydrates. In this study, identification and isolation of single-cell microalgae from Ambon Bay were carried out by serial dilution method and inoculation of the culture by appearance/spread on the surface of the solidified agar medium. The results showed that the waters of Ambon Bay have physical parameters such as temperature, pH, salinity, and TDS, which are still within the threshold specified in the seawater quality standard for biota. The water conditions at the 10 sampling locations had different salinity, pH, temperature, and TDS. This condition affects the distribution of microalgae in the waters in Ambon Bay. The image from light microscopy shows that there are 27 species of microalgae scattered in the waters of Ambon Bay, which belong to the classes Chlorophyceae, Flagilariophyceae, Bacillariophyceae (diatoms), Coscinodiscophyceae, and Dinophyceae. Microalgae isolation obtained five species (single-cell) including four species from the class Bacillariophyta (Navicula sp, Nitzschia sp, Cyclotella sp) and one species from the class Chlorophyta (Chlorella sp).
Chemicals Identification in The Ethyl Acetate Fraction and The Antioxidant Activity from Calabash Seed (Cresentia cujete) Extract Gaspersz, Nelson; Kapelle, Imanuel B. D.; Hattu, Nikmans; Sangadji, Insun; Arpipi, Henderika S. S.
HAYATI Journal of Biosciences Vol. 31 No. 6 (2024): November 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.6.1144-1153

Abstract

Calabash (Crescentia cujete) is a plant that grows in tropical climates and is scattered and easily found in Indonesia. Calabash is also known to contain several secondary metabolite compounds that have the potential as an antioxidant. This research is purposed to identify phytochemical compounds in calabash fruit seed extract, identify compounds based on the result of LC-MS, and know the antioxidant activity of calabash fruit seeds. The samples were extracted using the maceration method, and then phytochemical screening, LC-MS tests, and antioxidant tests were continued using the DPPH method. This research showed that the ethanol extract of calabash seed positive contains saponin, flavonoids, and triterpenoid compounds. Ethyl acetate extract of calabash seed contains flavonoids, alkaloids, and triterpenoids. The n-hexane extracts positively contain tannins, alkaloids, and steroids. The values of antioxidant activity of ethanol, ethyl acetate, and n-hexane extracts were IC50 = 29.7883, 7.219, and 848.712 ppm (µg/ml), respectively. Ethyl acetate extract as the best antioxidant activity was then tested with LC-MS, and results obtained from 26 compounds with the composition of the largest % area is convolvidine (11.277%), coumaroylquinic acid (8.290%) and taxchinin G (8,233%). They showed that calabash fruit seed extract has very strong antioxidant activity, with the best activity being the ethyl acetate extract of calabash fruit seed.
Analisis dan Karakterisasi Senyawa Silika (Sio2) Berbahan Dasar Batuan Vulkanik di Pulau Ambon Nggolaon, Delpina; Silahooy, Stevi; Jaya, Gede Wiratma; Hattu, Nikmans
Jurnal Kumparan Fisika Vol. 7 No. 3: Desember 2024
Publisher : Unib Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/jkf.7.3.98-105

Abstract

ABSTRAK   Penelitian ini bertujuan untuk melakukan analisis dan karakterisasi senyawa silika (SiO2) berbahan dasar batuan vulkanik di Pulau Ambon yang diekstraksi menggunakan metode kopresipitasi. Hal ini dikarenakan material senyawa silika merupakan salah satu material maju yang saat ini berperan pesat dalam dunia industri karena memiliki banyak keunggulan. Proses analisis dilakukan terhadap hasil pengujian karakterisasi menggunakan beberapa instrumen, antara lain XRF, XRD, dan SEM. Berdasarkan hasil uji XRF, serbuk silika mampu dimurnikan menjadi sebesar 47,40 wt %. Hal ini dikarenakan metode kopresipitasi mampu menghilangkan impuritas pengotor yang terkandung didalam batuan vulkanik. Kemudian untuk mengetahui fasa, struktur morfologi, dan ukuran partikel dari serbuk silika maka dilakukan uji XRD dan SEM. Berdasarkan hasil uji diketahui bahwa serbuk silika memiliki fasa amorf dengan posisi puncak  yang paling kuat pada sudut 2θ = 23.68˚. Hasil citra SEM menunjukkan adanya aglomerasi dan menghasilkan distribusi ukuran partikel tertinggi berada pada rentang  11-15 nm dengan ukuran partikel rata-rata sebesar 13,824 nm.   Kata  kunci—Batuan Vulkanik, Kopresipitasi, nano-Silika Amorf   ABSTRACT   This research aims to analyze and characterize silica compounds (SiO2) derived from volcanic rocks on the Ambon Island that were extracted using the co-precipitation method. This is because silica compound materials are one of the advanced materials that are currently playing a rapid role in the industrial world because they have many advantages. The analysis process was carried out on the results of characterization tests using several instruments, including XRF, XRD, and SEM. Based on the XRF test results, silica powder was able to be purified to 47.40 wt%. This is because the coprecipitation method is able to remove impurities contained in volcanic rocks. Then, to determine the phase, morphological structure and particle size of the silica powder, XRD and SEM tests were carried out. Based on the test results, it is known that silica powder has an amorphous phase with a strong board peak at at 2θ angles = 23.68˚. The results of the SEM image show that there is agglomerate and produce the highest particle size distribution in the range of 11-15 nm with an average size of 13.824 nm.   Keywords—Volcanic rocks, Co-Precipitation, Amorphous nano-Silica
Edukasi Pengelolaan Obat (DAGUSIBU) kepada Masyarakat Desa Asilulu untuk Meningkatkan Pengetahuan tentang Pengelolaan Obat yang Benar Pentury, Evangeline; Telussa, Ivonne; Hattu, Nikmans; Latupeirissa, Jolantje; Nusaly, Windy Natalia
Innovation for Community Service Journal Vol 3 No 1 (2025): April 2025
Publisher : Department of Chemistry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/icsj.v3i1.19133

Abstract

Swamedikasi merupakan praktik umum untuk mengatasi masalah kesehatan ringan tanpa konsultasi medis, namun mengandung risiko jika dilakukan dengan tidak tepat. Program pengabdian masyarakat dilakukan di Desa Asilulu dengan menggunakan pendekatan edukatif untuk meningkatkan kesadaran tentang penggunaan dan pengelolaan obat yang tepat. Program ini berfokus pada konsep DAGUSIBU (Dapatkan, Gunakan, Simpan, Buang) yang bertujuan untuk mendidik masyarakat tentang praktik mengelola obat yang tepat-mulai dari memperoleh obat dari sumber terpercaya hingga membuang obat yang sudah kedaluwarsa dengan aman. Kegiatan diawali dengan observasi lapangan untuk mengidentifikasi tingkat pengetahuan masyarakat terkait pengelolaan obat, diikuti dengan sesi penyuluhan interaktif dan pembagian materi edukasi. Hasilnya menunjukkan peningkatan pemahaman masyarakat tentang penggunaan obat yang rasional, termasuk penggunaan obat yang tepat, penyimpanan yang aman, serta pembuangan obat yang ramah lingkungan. Kesadaran masyarakat terhadap potensi bahaya dari pengelolaan obat yang tidak tepat juga meningkat. Kegiatan ini menunjukkan bahwa edukasi berbasis komunitas efektif dalam menumbuhkan perilaku rasional dalam penggunaan obat. Pentingnya hasil ini terletak pada kontribusi nyata terhadap peningkatan kesehatan masyarakat dan upaya menjaga kelestarian lingkungan melalui praktik pengelolaan obat yang benar
Synthesis and Characterization of Tripolyphosphate Chitosans through a Crosslinking Process from High Chemical Weight Chitosans Latupeirissa, Jolantje; Tanasale, Matheis F. J. D. P.; Fransina, Eirene G.; Hattu, Nikmans; Pada, Semuel S.; Sopamena, Helna Lourita
Jurnal Penelitian Pendidikan IPA Vol 11 No 6 (2025): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i6.10903

Abstract

This study aims to synthesize and characterize chitosan-tripolyphosphate (CTPP) through ionic crosslinking between chitosan and sodium tripolyphosphate. The CTPP powder was characterized using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and surface area analysis (BET), alongside solubility, swelling, and point of zero charge (pHZPC) tests. FTIR spectra showed a peak at 1151.5 cm⁻¹ attributed to P=O stretching, confirming the interaction between chitosan and tripolyphosphate. XRD analysis indicated shifts in diffraction peaks, suggesting a successful crosslinking reaction. SEM images revealed that the resulting particles had a compact and aggregated morphology. BET analysis showed a decrease in surface area compared to pure chitosan, which may be attributed to particle agglomeration upon crosslinking. Solubility tests revealed that while chitosan is soluble in acidic media, the CTPP product is insoluble. The CTPP showed a swelling percentage of 60% in acidic conditions, indicating its potential responsiveness. The pHZPC measurement indicated a value of 6.2, with ΔpH = 0, suggesting moderate surface charge behavior in neutral pH conditions. Overall, the study confirms the successful synthesis of crosslinked CTPP with distinct physicochemical properties suitable for further application development.  
Co-Authors Arpipi, Henderika S. S. Bijang, Chaterina Manukpadang Ervina Rumpakwakra Fensia Analda Souhoka Fransina, Eirene G. Gaspersz, Nelson Gede Wiratma Jaya Gede Wiratma Jaya, Gede Wiratma Hanoch J Sohilait Hellna Tehubijuluw Huliselan, Marsye Insun Sangadji Ivonne Telussa Kapelle, Imanuel B. D. Kololu, Micky Latupeirissa, Astrid Latupeirissa, Jolantje Marcia Violetha Rikumahu Nggolaon, Delpina Nusaly, Windy Natalia Pada, Samuel Simra Pada, Semuel S. Pentury, Evangeline Rosmawaty Harahap Sahalessy, Arielno Sekewael, Serly Jolanda Seumahu, Cecelia A Seumahu, Cecelia A. Siahaya, Antho Netty Silahooy, Stevi Sohilait, Mario Rowan Sopacua, Josina Sopamena, Helna Lourita Tanasale, Matheis F. J. D. P. Thohirah, Afifah Yusthinus T. Male