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All Journal BIOEDUKASI Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan JURNAL FARMASIMED (JFM) Indonesia Natural Research Pharmaceutical Journal (INRPJ) Pharmacy Action Journal KAMI MENGABDI Indonesian Journal of Pharmaceutical Research
Panjaitan, Riong seulina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Public Health Social Sciences Transportation Other

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Extraction, Phytochemical Profiling, and Antibacterial Activity of n-Hexane Crude Extracts of Macroalgae Padina australis Lenoera, Agnes; Panjaitan, Riong Seulina; Purwati, Purwati
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.51123

Abstract

Padina australis, brown macroalgae, has potential bioactive compounds that possess remarkable bioactivities including antimicrobial. The present study extracts macroalgae P. australis using Ultrasound assisted extraction (UAE) with n-hexane solvent, followed by screening phytochemical compounds. Moreover, the antimicrobial activity and MIC value of the n-hexane crude extracts of Padina australis was tested against Shigella sonnei, Escherichia coli, Salmonella typhi, and Bacillus cereus by using the disc diffusion method. This result demonstrates the optimum time of UAE process is at 10 minutes that yields 0.117 %. The phytochemical screening of n-hexane crude extracts of Padina australis macroalgae revealed the presence of secondary metabolites such as alkaloids, flavonoids, saponins, tannins, steroids and phenols. Antibacterial activity of this extract against Shigella sonnei, Escherichia coli, Salmonella typhi, and Bacillus cereus at 100 ppm concentration with inhibitory diameter range of 10.97 to 13.86 mm. Then, the n-hexane crude extracts of P. australis is potent against Bacillus cereus with MIC value of 6.25 ppm.
Identification, Characterization and Antifungal Activity Test of Chemical Compounds from Methanol Extract of Macroalgae Padina australis Against Dandruff Fungus Malassezia globosa Djohansah, Violetta; Panjaitan, Riong Seulina; Zuraida Sagala
Jurnal FARMASIMED (JFM) Vol 8 No 1 (2025): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/3mat6358

Abstract

Padina australis is a species of brown macroalgae with potential as a natural source of bioactive compounds, including antifungal agents. This study aimed to identify the functional groups present in the methanolic extract of Padina australis using Fourier Transform Infrared (FTIR) spectroscopy and to evaluate its antifungal activity against Malassezia globosa through the disk diffusion method. FTIR analysis revealed the presence of hydroxyl (3433.41 cm⁻¹), aliphatic C–H (2916.47 cm⁻¹), carbonyl (1735.99 and 1643.41 cm⁻¹), amine (1573.97 and 1242.20 cm⁻¹), C–O, and carboxylate (COO⁻) groups, indicating key secondary metabolites such as flavonoids, phenols, esters, amines, and polysaccharides. Antifungal testing showed that the extract at a concentration of 100 ppm produced the largest inhibition zone (16.58 ± 4.71 mm), while the minimum inhibitory concentration (MIC) was determined at 12.5 ppm with an inhibition zone diameter ≥12 mm. These findings suggest that Padina australis holds promising potential as a natural source for developing antifungal therapies against Malassezia globosa.
Co-Authors Afikah, Hana Tri Anastasya, Millenia Putri Andrian, Rahmat Ardian, Kristian Derius Aslawiyah, Siti Rabiaatul Asriyanti, Laila Safa Djohansah, Violetta Elawati, Nur Febriani, Ike Ghassani, Fatiha Azka Hafiz, Muhammad Ariqoh Langit, Vinsensia Asahi Sulan Lenoera, Agnes Meze, Maria Fortunata Nurwijayanti parma, syafriadi kurnia Pradipta, Oki Purwati Purwati Putranto, Haidar Dwi Putri, Hervita Reza, Fakhrur Sabrina, Rorina Sagala, Zuraida Saiful Saiful Salsabilla, Zuli Astuti Septiyani, Anisah Simanjuntak, Yulia Verawati Simatupang, Angel Maria Sinambela, Rio Pandi Siti Aisyah Suciati, Lily Sumantri Sumantri Suryo, Nabila Dwi Wijaya, Bagas Eva Yosepin, Elpina Yulius Evan Christian Zuraida Sagala