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Journal : Majalah Obstetri dan Ginekologi

Effect of oocyte vitrification before and after in vitro maturation towards Bcl-2, Bax and Bcl-2/Bax ratio expression Zakiyatul Faizah; R. Haryanto Aswin; Hamdani Lunardhi
Majalah Obstetri dan Ginekologi Vol. 24 No. 2 (2016): May - August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.057 KB) | DOI: 10.20473/mog.V24I22016.56-60

Abstract

Objectives: to compare the expression of Bcl-2, Bax and Bcl-2/Bax ratio in cumulus cell and oocyte between vitrified oocyte pre and post in vitro maturation.Materials and Methods: Maturation was operated in medium TC 100 µl for 24 hours. Vitrification begins with washing oocyte in PBS basic medium supplemented of 20% serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing is processed by submerging the oocytes in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2). PBS + 20% serum + 0.25 M sucrose, and 3). PBS + 20% serum + 0.1 M sucrose. Imunocytochemistry observed the expression of Bcl-2, bax and Bcl-2/bax ratio.Results: Bcl-2 expression on oocyte in control group differed significantly with treatment group, Bcl-2 expression on cumulus in control group differed significantly with treatment 1 group. Bax expression on oocyte in control group differed significantly with treatment group. Bax expression on cumulus in control group differed significantly with treatment group. Bcl-2/Bax expression ratio on oocyte and cumulus did not differ significantly in all groupConclusion: No difference Bcl-2/Bax expression ratio on oocyte and cumulus between vitrified oocyte pre and post in vitro maturation.
Comparison of pregnancy rates on day 3 and day 5 embryo transfer in In Vitro Fertilization (IVF) Audia Mumtaz Rifasky; Puspa Wardhani; Ashon Sa’adi; Ninik Darsini; Hamdani Lunardhi; Zakiyatul Faizah
Majalah Obstetri dan Ginekologi Vol. 29 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V29I12021.14-17

Abstract

Objectives: To identify the success rates of pregnancy on the third and fifth day embryo transfer at Graha Amerta Hospital, Surabaya, Indonesia.Materials and Methods: This study used comparative cross sectional design. Data were taken from medical record of IVF participants who met the inclusion and exclusion criteria at Graha Amerta Hospital for the period of January 2016 - December 2016.Results: Successful pregnancy rates were found in this research. The embryo transfer on the third day and the fifth day were 35% and 49.3% respectively. In other words, the rates of pregnancy success were not affected by embryo transfer on the third day and the fifth day in the medical record sample as it had p value of 0.090.Conclusion: Embryo transfer on the third and fifth days had the same rates of pregnancy success in IVF participants at Graha Amerta Hospital, Surabaya, Indonesia. 
Increased vulvovaginal candidiasis incidence risk in various gestational ages at a private midwifery practice in Surabaya, Indonesia Regina Martina Cilik; Willy Sandhika; Zakiyatul Faizah
Majalah Obstetri & Ginekologi Vol. 30 No. 2 (2022): August
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mog.V30I22022.72-77

Abstract

HIGHLIGHTS 1. Hormonal changes during pregnancy lead to the presence of vulvovaginal candidiasis.2. Increased incidence of vulvovaginal candidiasis in different pregnancy trimesters were analyzed.3. Frequency of vulvovaginal candidiasis tends to increase along with the gestational age.   ABSTRACT Objectives: This study aimed to analyze whether there was an increase in the incidence risk of vulvovaginal candidiasis at various gestational ages. Materials and Methods: A study examined vaginal smears of pregnant women at a private midwifery practice Farida Hadjri, Surabaya, Indonesia. A total sample of 96 patients was taken by the consecutive sampling method, consisting of 32 patients each from the 1st, 2nd, and 3rd trimester of pregnancy.y The smear was examined using Gram stain at Balai Besar Laboratorium Kesehatan Daerah Jawa Timur to detect the presence of fungi elements. Data were analyzed using Chi-square test with 95% confidence interval to investigate whether there was an increased risk of vulvovaginal candidiasis and gestational age. Results: There was an increased risk of vulvovaginal candidiasis and increased gestational age (p < 0.05). This result was related to the hormonal status of pregnant women. An increase in estrogen level during pregnancy leads to an increase in glycogen production by vaginal epithelial cells, which cause the vaginal environment to become more susceptible to fungal growth. Conclusion: There was increased vulvovaginal candidiasis incidence risk along with gestational age. Pregnancy is one of the predisposing factors for candida infection.
Co-Authors Agde Muzaky Kurniawan Agustinus Agustinus Agustinus Aldila Diah Rumiyandini Almira Nadya Yasmine Anggi Senda Nikenindya Anggis Putri Wijayanti Annas, Jimmy Yanuar Archangela Olivia Eugenia Ardiar Rahmannanda Laksanadi Ashon Sa’adi Aswin, R. Haryanto atika Atmi Marmiati Aucky Hinting Audia Mumtaz Rifasky Azhar Nur Fathoni Bella Amanda Budi Prasetyo Cahya Ramadhanti Putri Cantika Erningtyas Cherryl Aurelia Chitra Devi Paramita Prasetyawati Danial Habri Arsy Dessy A Ningsi Dewi Sulistyawati Dhien Meutia Islamadina Wulandani Edgar Alief Angkasa Putra Eighty Mardiyan K, Eighty Eko Budi Siswidiyanto Eko Budi Siswidiyanto Endyka Erye Frety Esti Isyroofanaa Falerina, Rosa Farah Ayu Noviannisa Fayka Putri Poempida Ferdinandus, Euvanggelia Dwilda Hamdani Lunardhi Hanifah Puspita Nurul H HERAWATI, LILIK Irwanto Irwanto Jayanti, Ratna Dwi Josephine Queena Maureen Judie Hartono Judie Hartono Latiful Elisia Martono Tri Utomo Mochammad Ridhwan Soediono Muhammad Fadhil Kamaruddin Muhammad Ircham Gustriawan Ninik Darsini Nugroho, Puguh Setyo Nur Rohmah Nurul Hikma Pandya Daniswara Winantyo Permata Aprilia Putri Pety Narulita PUNGKY MULAWARDHANA, PUNGKY Puspa Wardhani Puteri Reno Fadila Rangga Panengah B Regina Martina Cilik Relly Yanuari Primariawan, Relly Yanuari Rimbun Rize Budi Amalia Rize Budi Amalia Rizka Fathoni Perdana Salsabila Salsabila Sang Gede Farell Iswara Perdana Saskia Ramadhanti Shafira Meidy safitri Sitisalma Amirah Dzakiyyah Sri Musta'ina Tri Hartini Yuliawati Wahyulia Rizqia Umami Wahyuni, Briliansa Septi Dwi Widjiati Widjiati William William Williana Suwirman Williana Suwirman Willy Sandhika Yuni Hisbiyah Zalfa Qanita