Rosmaina Rosmaina
Department Of Agrotechnology, Faculty Of Agriculture And Animal Husbandry, Universitas Islam Negeri Sultan Syarif Kasim, Pekanbaru, Riau 28293, Indonesia

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Journal : JURNAL AGROTEKNOLOGI

INDUKSI KALUS PASAK BUMI (Eurycoma longifolia Jack) MELALUI EKSPLAN DAUN DAN PETIOL ULFIATUN, ULFIATUN; MAISUPRATINA, MAISUPRATINA; ROSMAINA, ROSMAINA; ZULFAHMI, ZULFAHMI; SUTEJO, PROBO
JURNAL AGROTEKNOLOGI Vol 6, No 1 (2015): Agustus 2015
Publisher : JURNAL AGROTEKNOLOGI

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of the problem of Eurycoma longifolia Jack propagation was low germination percentage due to recalcitrant seeds and slow growth of seedling from cutting propagation. To overcome this problem is required propagation of Eurycoma longifolia via in vitro culture. The objective of this research was to know the effect of Auxin (2,4-D and NAA) and Cytokines (BAP and Kinetin)  on Eurycoma longifolia callus induction via leaf and petiole explants. In this study, we used plant growth regulator of 2,4 D, NAA, BAP and Kinetin in several levels.  The observed variables were appearing callus time, callus color and callus texture. The results of this study showed that MS medium supplemented with 1 ppm NAA+ 1 ppm BAP was able to induce callus formation in leaf explant for 6 months after culture. While MS medium supplemented with 1 ppm 2,4-D, 1 ppm BAP, combination of 2,4-D and Kinetin and combination of 2,4-D and BAP can induce callus formation from petiole. All the callus formation has yellow color and yellow brown color. The petiole explant that is grown in MS medium supplemented with 1 ppm BAP induced of callus in short time (18 days after culture).
KEPADATAN DAN POLA PENYEBARAN PASAK BUMI (Eurycoma longifolia Jack) DI ZONA ALAMAN KUYANG, HUTAN LARANGAN ADAT KENEGARIAN RUMBIO ZULFAHMI ZULFAHMI; NELAWATI NELAWATI; ROSMAINA ROSMAINA
Jurnal Agroteknologi Vol 6, No 1 (2015): Agustus 2015
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v6i1.1375

Abstract

The objective of this research was to know the density and distribution pattern of E. longifolia in the Alaman Kuyang zone of the forest reserve of Kenegerian Rumbio. The research used purposive sampling method. The observation plot is made in square plot  which plot size was 20 x50 m.  Results of this study showed that density  of E. longifolia in Alaman Kuyang zone of forest reserve of Kenegarian Rumbio was 130 individual ha-1, which seedling phase is more dominance than  sapling and pole phases. The density of E.longifolia was low due to increasing the harvesting of this species from forest reserve of Kenegarian Rumbio, and addition poor of mother trees in area study. Distribution pattern of E. longifolia were clumped with Morisita Index value > 1, this could be explained since seed of E. longifolia dispersed and grew not far from mother trees.
LAJU MULTIPLIKASI TUNAS NENAS (Ananas comosus L. Merr) PADA MEDIA DASAR MURASHIGE AND SKOOG HASIL PERLAKUAN BA DAN NAA SECARA IN VITRO Rosmaina Rosmaina
Jurnal Agroteknologi Vol 1, No 1 (2010): Agustus 2010
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v1i1.49

Abstract

The objectives of this research are to study shoots multiplication rate of Pineapple (Ananas comosus L. Merr) on Murashige and Skoog (MS) basal medium from BA, NAA and its combination treatment by in vitro culture. The experimental design was completely randomize design with two factorials. The first factor is BA consists of 0.00 μM, 4.44 μM, 8.88 μM, 13.32 μM and 17.76 μM, and the second factor is NAA consist of 0.00 μM, 0.50 μM, 1.00 μM and 2.00 μM. The result of this study showed that BA, NAA and its combination could increase the shoot multiplication rate of pineapple on basal medium Murashige and Skoog. BA and NAA combination produce high multiplication rate than BA without NAA or NAA no added of BA. The best treatment in this study was 4.44 mM NAA + 0.5 mM NAA, produce 107.001,20 planlets/year in Murashige and Skoog basal Medium with uniform, normal and healthy planlet.
Keanekaragaman Tanaman Kantong Semar (Nepenthes spp.) di UIN SUSKA Riau Pitcher Plant (Nepenthes spp) Diversity in the UIN SUSKA RIAU Rosmaina Rosmaina; Zulfahmi Zulfahmi; Rizka Rosa
Jurnal Agroteknologi Vol 2, No 2 (2012): Februari 2012
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v2i2.126

Abstract

Forest of the UIN SUSKA Riau was harboring most of the genetic resources, one of them is pitcher plants (Nepenthes spp). Pitcher plants have high economic value because of they were ornamental and medicinal plants. This research aims to determine population density, diversity index, evenness index and dominance index of Nepenthes in the campus forest of UIN SUSKA Riau. This research used method was purposive sampling with square Jalur method. The result of this study found two species of Nepenthes in the campus of UIN SUSKA Riau, namely Nepenthes gracillis and Nepenthes ampularia. The average of population density of Nepenthes gracillis and Nepenthes ampularia were 2000 individuals/ha and 200 individuals/ha, respectively. The Diversity index of Nepenthes in this study was low (H’ <1), while average of evenness index (E) of Nepenthes was 0.352.
Pengaruh Perlakuan BA dan NAA terhadap Pembentukan Akar Nenas (Ananas comosus (L). Merr.) cv. Smooth Cayenne Secara In Vitro Rosmaina Rosmaina
Jurnal Agroteknologi Vol 1, No 2 (2011): Februari 2011
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v1i2.55

Abstract

The objectives of this research are to study the effect of shoot multiplication media and subculture on root formed and survive of plantlet during acclimatization. Plant material was used that explants of pineapple (Ananas comosus (L.) Merr.) cv Smooth Cayenne which resulting from multiplication with several concentrations of BA and NAA which are subculture two times. The results of this research showed that all treatments of multiplication medium do not difference significance on explants capability to root formed and survive during acclimatization, but re-subculture effect on plantlet survival during acclimatization which all treatments at first subculture showed result better than second subcultures. It’s showed
ANALISIS KEKERABATAN GENETIK TANAMAN PADI (Oryza sativa L.) DI KABUPATEN KAMPAR DENGAN MENGGUNAKAN PENANDA Random Amplified Polymorphic DNA (RAPD) Rita Elfianis; Joko Warino; Rosmaina Rosmaina; Suherman Suherman; Zulfahmi Zulfahmi
Jurnal Agroteknologi Vol 11, No 2 (2021): Februari 2021
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v11i2.10013

Abstract

Rice is a cereal crop that belongs to the graminae family of high economic value, and is the main food for more than a portion of the world's population. This study aims to determine the relationship genetic of rice in Kampar by RAPD marker. Eight genotypes of rice from district in Kampar were amplified using eight primers (OPA 5, OPB 7, OPC 19, OPD 2, OPD 3, OPD 8, OPD 11, and OPD 13). The analysis of molecular data was performed by using NTSys pc -2.02 and GenAlex 6.1 . The results of the amplification of rice produced 48 loci with DNA band sizes obtained ranging from 350-1700 bp. The average percentage of polymorphic loci of the eight primers was 49.80%, where the highest percentage of polymorphic loci produced by OPA-7 primer was 75%, while the lowest polymorphic locus percentage was observed in OPD-13 primer which was 14.28%. The value of genetic distance between rice from Kampar in this study is in the range of 0.06 - 0.37. At a genetic distance of 0.79, rice plants can be grouped into two groups, namely the first group consisting of sokan and coku. The second group consisted of suntiong, korean , cupak putio, kuniong, jangguik, and cupak tenggi. The results of this study are expected to be taken into consideration in developing rice breeding strategies in the future.
EKSPLORASI DAN KARAKTERISASI KANTONG SEMAR (Nephentes sp.) DI KAMPUS UIN SUSKA RIAU Rosmaina Rosmaina; Zulfahmi Zulfahmi
Jurnal Agroteknologi Vol 2, No 1 (2011): Agustus 2011
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v2i1.132

Abstract

Pitcher plant (Nepenthes sp) is a member of Nepenthaceae family. In Riau Province, pitcher plant is known locally as Periuk monyet. This species is economically important due to used as ornamental and medicine plants. In the present study, we want to know the kind of pitcher plant in peat swamp forest of State Islamic University of Sultan Syarif Kasim (UIN SUSKA) Riau. The result of exploration found that there are four species of Nepenthes growth in peat swamp forest of UIN SUSKA Riau, namely: N. ampularia, N. gracillis, N. mirabilis, and one species is estimated hybrid between N. ampularia x N. gracillis (Nepenthes x trichocharpa). The number of hybrid between N. ampularia x N. gracillis is found restricted, so that it is required another study to ensure that phenomenon.
KERAGAMAN GENETIK MUTAN M-2 CABAI MERAH KERITING (Capsicum annuum L.) BERDASARKAN PENANDA RAPD Rosmaina Rosmaina; Dedi Mulyadi; Rita Elfianis; Zulfahmi Zulfahmi
Jurnal Agroteknologi Vol 10, No 2 (2020): Februari 2020
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v10i2.8780

Abstract

Chili is an important horticultural plant in Indonesia. This research aims to carry out RAPD analysis on Mutant M2 of chili pepper (Capsicum annuum L.). Six M2 genotypes of chili irradiated by gamma ray and control plants were amplified by 16 random primers. The amplification results of M2 chili with 16 primers produced 118 loci, with fragment sizes ranging from 150-2000 bp. The number of polymorphic loci was 96 loci and the percentage of polymorphic loci was 83.23%. The DNA fragment polymorphism produced in this research was relatively high and it showed that the gamma ray mutagen applied produced high chili genetic diversity. The value of genetic similarity between control plants and mutant plants ranged from 0.7474 to 0.4874. UPGMA dendogram classified seven genotypes tested into three groups, the first group consisted of mutants R2U6 and R2U17, the second group was mutants R1U14 and R1U17, and the third group was mutants R2U8, mutants R2U2 and control plants. The finding of this research can be used as a basic selection of genetic material for chili’s breeding in the future.
INDUKSI KALUS PASAK BUMI (Eurycoma longifolia Jack) MELALUI EKSPLAN DAUN DAN PETIOL ROSMAINA ROSMAINA; ZULFAHMI ZULFAHMI; PROBO SUTEJO; ULFIATUN ULFIATUN; MAISUPRATINA MAISUPRATINA
Jurnal Agroteknologi Vol 6, No 1 (2015): Agustus 2015
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v6i1.1567

Abstract

One of the problem of Eurycoma longifolia Jack propagation was low germination percentage due to recalcitrant seeds and slow growth of seedling from cutting propagation. To overcome this problem is required propagation of Eurycoma longifolia via in vitro culture. The objective of this research was to know the effect of Auxin (2,4-D and NAA) and Cytokines (BAP and Kinetin)  on Eurycoma longifolia callus induction via leaf and petiole explants. In this study, we used plant growth regulator of 2,4 D, NAA, BAP and Kinetin in several levels.  The observed variables were appearing callus time, callus color and callus texture. The results of this study showed that MS medium supplemented with 1 ppm NAA+ 1 ppm BAP was able to induce callus formation in leaf explant for 6 months after culture. While MS medium supplemented with 1 ppm 2,4-D, 1 ppm BAP, combination of 2,4-D and Kinetin and combination of 2,4-D and BAP can induce callus formation from petiole. All the callus formation has yellow color and yellow brown color. The petiole explant that is grown in MS medium supplemented with 1 ppm BAP induced of callus in short time (18 days after culture).
STUDI PENGARUH MEDIA ALTERNATIF UNTUK PERBANYAKAN PISANG BARANGAN (Musa acuminata L.) SECARA IN-VITRO Rosmaina Rosmaina; Ragil Endika; Zulfahmi Zulfahmi
Jurnal Agroteknologi Vol 12, No 1 (2021): Agustus 2021
Publisher : Universitas Islam Negeri Sultan Syarif Kasim Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24014/ja.v12i1.12425

Abstract

The main obstacles in the commercialization of seedlings mass propagation through tissue culture techniques is the high cost of the culture media component. therefore, the production of lowcost tissue culture is required. This study aims to develop low cost in-vitro media for the production of the seedlings of Barangan banana. The research was composed following Factorial Completely Randomized Design, the first factor was Terra Novalgro liquid fertilizer with three concentrations, namely 1, 2, and 3 ml L-1, while the second factor was Gandasil with 3 concentrations namely 1, 2, and 3 mg L-1, so obtained nine treatments, each treatment was repeated 10 times, so that there were 90 experimental units. MS media was used as control. The parameter observed was number of shoots, number of leaves and number of roots. The results of this study exhibited that the treatment of 1 ml L-1 liquid fertilizer + 2 mg L-1 foliar fertilizer produced 9.30 shoots/explant and 1.90 leaves/explant, that no significantly different from MS medium (control) which produced 9.0 shoots/explant and 0.3 leaves/explants. Therefore, the using completed liquid fertilizers and foliar fertilizers as medium in vitro propagation of barangan bananas can become an alternative replace MS medium, as well as reduced the cost of culture media as 91% -93% compared to MS media.