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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Pusat Inovasi Masyarakat
Kusumah, Yayi Munara
Departemen Proteksi Tanaman, Fakultas Pertanian, IPB University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Social Sciences

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Analisis filogenetik Hyposidra talaca nucleopolyhedrovirus (HytaNPV) yang diisolasi dari perkebunan teh Gunung Mas, Bogor, Jawa Barat dan virulensinya terhadap Hyposidra talaca Walker: Phylogenetic analysis of Hyposidra talaca nucleopolyhedrovirus (HytaNPV) isolated Gunung Mas tea plantation from Bogor, West Java and its virulency against Hyposidra talaca Walker Kusumah, Yayi Munara; Kurniawati, Fitrianingrum; Kristanto, Eka Dana; Parasian, Franciskus; Christian, Michael
Jurnal Entomologi Indonesia Vol 20 No 2 (2023): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.20.2.151

Abstract

Hyposidra talaca (Walker) is an important pest of tea plant. H. talaca can cause losses of between 40–100% in the dry season if proper control is not carried out. H. talaca has natural enemies such as predators, parasitoids, and pathogens. One of the entomopathogens is NPV. This study aims to obtain molecular characteristics through DNA polymerase sequences and determine the virulence level of NPV isolates from H. talaca. The same species from different locations can have genetic variability. Therefore, molecular characterization by polymerase chain reaction (PCR) on DNA polymerase sequences is one way to study the genetics of HytaNPV. NPV was isolated from infected H. talaca larvae collected from the field. The DNA isolates were used as templates for PCR for DNA polymerase gene amplification with an amplicon target of ±1,000 bp. A sequencing process followed the PCR provides nucleotide sequence. HytaNPV DNA polymerase sequencing results were aligned with GenBank's BLAST data to provide information on the relationship of HytaNPV to NPVs isolated from other regions. Based on molecular character analysis using DNA polymerase gene sequence, HytaNPV Bogor has a homology level of 93.9% with HytaNPV isolated from India. HytaNPV Bogor has a genetic relationship with the NPV that infects Buzura suppressaria from China and Australia. HytaNPV Bogor is similar to the NPV that infects H. talaca from India. The bioassay of HytaNPV isolate against H. talaca showed the highest LT50 value of 1.92 days was found in concentration of 1.58 x 107 POBs/ml in second instar larvae.
Molecular characterization of Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) on Helicoverpa armigera Hübner larvae (Lepidoptera: Noctuidae) Kusumah, Yayi Munara; Kurniawati, Fitrianingrum; Pramudita, Pajar; Christian, Michael
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 2 (2024): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.224243-250

Abstract

Nucleopolyhedrovirus (NPV) is a type of biological agent that can be effectively used as a bioinsecticide to control the cob borer caterpillar in corn. Among these viruses, HearNPV (Helicoverpa armigera Nucleopolyhedrovirus) stands out due to its host specificity, environmentally safety, and lack of harm to non-target organisms. The primary objective of this study was to elucidate the molecular characteristics of the DNA polymerase gene of HearNPV. To achieve this, caterpillars were collected from the field, then reared and inoculated in the laboratory under controlled conditions. Following propagation, viral genomic DNA was extracted using a modified CTAB (Cetyltrimethylammonium Bromide) protocol, which is commonly employed for its efficiency in isolating high-quality DNA from various sources. Subsequently, DNA amplification was performed using specific primers: forward primer HearNPV F and reverse primer HearNPV R. These primers were designed to target the DNA polymerase gene, a crucial component in viral replication and a key marker for phylogenetic studies. The results of the phylogenetic analysis indicated that the Bogor strains of HearNPV shared a high degree of similarity with NPVs that infect other species within the Helicoverpa genus, including strains from Spain, Australia, Brazil, Russia, and Japan. Specifically, the nucleotide sequence homology ranged from 98.4% to 99.4%, while the amino acid sequence homology ranged from 98.2% to 99.2%. This high level of homology suggests a close evolutionary relationship and potentially similar biological characteristics among these NPV strains.
Identifikasi molekuler lepidopteran predator Coccidae pada tanaman kopi di Sukabumi: Molecular identification of predatory Lepidoptera of Coccidae on coffee plants in Sukabumi Arlia Dwi Hapsari; Sugeng Santoso; R. Yayi Munara Kusumah
Jurnal Entomologi Indonesia Vol 21 No 2 (2024): July
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.21.2.151

Abstract

A predatory Lepidoptera that has the potential to be a biological control agents of the soft scale insect (Coccidae) on coffee plants (Coffea spp.) was found in Sukabumi, West Java. However, information regarding the species name of predatory Lepidoptera has never been reported in Indonesia. Literature for morphological identification of this predatory Lepidoptera species is limited. Therefore, this study was conducted to identify predator species molecularly. Predators were collected from coffee plantations that were infested by soft scale in Sukabumi, West Java. Identification using the PCR procedures of mitochondrial COI gene was carried out in the laboratory. The results show that the sample DNA sequences (629 bp) has a similarity level of 95.67% (GenBank) and 95.37% (BOLD Systems) to the Autoba rubra Hampson species. Based on phylogeny analysis, the sample is also closely related to Mataeomera spp. The DNA sequences alignment variation of sample and four species voucher A. rubra in the GenBank database was 7.58%. Therefore, it can be concluded that the predatory Lepidoptera preying on soft scale insects on coffee plants in Sukabumi are most closely related to A. rubra.
Genetic structure analysis of several peroral infectivity factor gene in Spodoptera litura Nucleopolyhedrovirus Christian, Michael; Kusumah, Yayi Munara; Mutaqin, Kikin Hamzah
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 1 (2025): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.125128-140

Abstract

Spodoptera litura Nucleopolyhedrovirus (SpltNPV) is an entomopathogenic virus from the Baculoviridae family, currently under development as a biological control agent for cutworm Spodoptera litura. The NPV’s ability to infect its pest host can be determined by expression of pif protein complex by pif gene. The research aims to acquire genetic character information of the pif gene of SpltNPV from Bogor. Amplification of the NPV gene was carried out using the specific primers to amplifies two types of pif gene. The PCR products were sequenced then the DNA sequences were analyzed with the BioEdit and BLAST programs. The PCR amplification results showed that the size of the sample DNA fragment was 900 bp, 1300 bp and 710 bp. Based on the sequence analysis results, SpltNPV isolates from Bogor are closely related to SpltNPV and SpliNPV isolates from China. The highest nucleotide homology values of Pif-1, Pif-2, and Pif-3 gene were 99.56%, 99.37% and 100%, respectively. Based on the results of phylogenetic analysis, HearNPV isolates from Bogor belong to the same group as the NPVs that infect the species Spodoptera litura and closely related to NPV that infect Spodoptera littoralis. The amino acid sequence analysis showed the number of mutated sites in pif-1, pif-2, and pif-3 in this study are 1, 3, and 0, respectively, which indicates that protein mutation in SpltNPV Bogor did not significantly alter the viral infection process.
Aktivitas insektisida ekstrak Piper aduncum dan Aglaia odorata terhadap Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae): Insecticidal activity of Piper aduncum and Aglaia odorata extracts on Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) heviyanti, Maria; Dadang; Sartiami, Dewi; Kusumah, Yayi Munara; Purwantiningsih
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.52

Abstract

One of the main problems in the cultivation of Brassicaceae vegetable plants is the attack of diamondback moth Plutella xylostella (Linnaeus). Extracts of Piper aduncum and Aglaia odorata have the potential to be used as botanical insecticides because they contain compounds that are toxic to insects. This study aims to evaluate the toxicity and feeding inhibition activity of hexane-extract of P. aduncum fruits and methanol-extract of A. odorata twigs against second instar larvae of diamondback moth. Extraction was carried out using the maceration method with a powder-to-solvent ratio of 1:10 (w/v). The diamondback moth insects used were from colonies reared in the laboratory. The lethal effect test was conducted using the leaf-dip method with test concentrations equivalent to LC15, LC35, LC55, LC75, LC95, and a control. The feeding inhibition test was conducted using the choice-test method with test concentrations equivalent to LC15, LC35, LC55, and a control. The results show that the hexane-extract of P. aduncum fruit is more toxic to second instar P. xylostella larvae than the methanol-extract of A. odorata twigs based on the probit analysis value of the relationship between concentration and mortality. The LC50 and LC95 values of P. aduncum extract were 0.07% and 0.14%, respectively, while the LC50 and LC95 values of A. odorata extract were 1.63% and 4.72%. Additionally, P. aduncum and A. odorata extracts also exhibited feeding against diamondback moth at concentrations of 0.07% and 1.77%, respectively. The combined effects of direct toxicity and feeding inhibition contributed to the mortality of the test insects. Thus, these two extracts, escpecially P. aduncum extract wich demonstrated higher effectiveness, have a great potential as botanical insecticides for controlling diamondback moth in Indonesia.
Keanekaragaman strain ulat grayak jagung Spodoptera frugiperda (Smith) berdasarkan marker COI dan Tpi dengan kajian kepadatan populasi di Bali: Genetic diversity of fall armyworm Spodoptera frugiperda (Smith) strains based on COI and Tpi markers with population density analysis in Bali Monica, Dheya Cintya; Kusumah, Yayi Munara; Winasa, I Wayan
Jurnal Entomologi Indonesia Vol 22 No 1 (2025): March
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.22.1.41

Abstract

Spodoptera frugiperda (Smith), known as fall armyworm (FAW), originating from America that devastates corn crop, causing up to 50% damage to corn crops in Bali. Molecular identification of strain diversity using mitochondrial cytochrome oxidase subunit I gene (mtCOI) and triosephosphate isomerase gene (Tpi) markers also population density monitoring are a fundamental method for monitoring, detecting and controlling these pests. This research aims to determine FAW strain based on COI and Tpi markers and their population density analysis at representative sites in Bali. This study involved several stages: samples collection; DNA extraction; amplification, visualization; Population density observation and sequencing; and data analysis. Data were analyzed using GeneStudio and BioEdit for editing and alignment, and MEGA 11 for constructing phylogenetic tree. Sequence analysis based on COIB indicated that samples from Bali comprised of 41.67% rice strains and 58.33% corn strains, with 100% of corn strain  haplotypes being h4 FAW [FL] from Florida. Based on Tpi marker, 100% of the samples were identified as corn strains with haplotypes Ca1 and Ca2. This study found that the FAW diversity in Bali consists of two strains and one haplotype based on COI (COI-R and COI-Ch4), while based on Tpi, there is only one strain  with two haplotypes (Tpi-Ca1 and Tpi-Ca2). The highest population density of FAW was observed in the Tabanan plot, with 6,8 larvae/sample unit. Early instar larvae (2–3) were predominant in 3 WAP whereas late instar larvae (4–6) were predominant in 5 WAP.
Policy Priorities for Disseminating Biopesticide in Chili Farmers Nanta, Wahyu Ridwan; Wiyono, Suryo; Kusumah, Yayi Munara
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 3 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.3.568

Abstract

Controlling pests and diseases of chili plants still relies on synthetic chemical pesticides, which have negative effects on agroecosystems. Biopesticides can be used as alternatives to synthetic pesticides. The use of biopesticides in Indonesia is still low, and popularization efforts are required. The government plays a role in determining policies that encourage the popularization of biopesticides. This study aimed to determine policy priorities for the popularization of biopesticides for chili farmers. Policy priorities are determined using the analytical hierarchy process (AHP) method, which allows the identification of priority criteria and priority sub-criteria. The current policy on crop protection is insufficient to encourage the popularization of biopesticides. Low commercialization and government programs that are not yet massive are indications of the low use of biopesticides. The AHP results showed that the criteria for knowledge about biopesticides are policies that must be prioritized, where the sub-criteria of the level of intensity and quality of extension are the top priorities. The effectiveness and efficiency policy criteria need to prioritize the effectiveness of sub-criteria in controlling pests and diseases over the efficiency of use, preservation of natural enemies, and product quality, while the ease of obtaining products is the main factor in prioritizing the availability and affordability criteria. Keywords: analytical hierarchy process, AHP, biopesticide, farmers, IPM, policy
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Munif Arlia Dwi Hapsari BUDI TJAHJONO Christian, Michael Dadang DADANG DADANG DAMAYANTI BUCHORI DEWI SARTIAMI Efi Toding Tondok Fahira Hanifah Fajrin Fahmi Farriza Diyasti Fitrianingrum Kurniawati, Fitrianingrum Heviyanti, Maria I Wayan Winasa Kikin H Mutaqin Kristanto, Eka Dana Monica, Dheya Cintya Nanta, Wahyu Ridwan Nur Rachman Parasian, Franciskus Pramudita, Pajar PURNAMA HIDAYAT Purwantiningsih RAUF, AUNU Revi, Lestia RUSMILAH SUSENO Samsudin Samsudin Samsudin Samsudin Sugeng Santoso Suryo Wiyono Teguh Santoso Teguh Santoso Trendy Hartanto