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All Journal Microbiology Indonesia JURNAL BIOLOGI INDONESIA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Jurnal Pengabdian Masyarakat Mandira Cendikia (JPKM-MC) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Martgrita, Merry Meryam
Institut Teknologi Del
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Mechanical Engineering Medicine & Pharmacology Social Sciences Other

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EFFECT OF STORAGE TIME VARIATION OF ANDALIMAN FRUIT ON FREE RADICAL SCAVENGING AND PROLIFERATION INHIBITION ON MCF-7 CELLS Rosidah, Idah; Kusumastuti, Siska Andrina; Simanungkalit, Grace Sophiwati; Rahayu, Maya Damayanti; Nuralih, Nuralih; Martgrita, Merry Meryam; Kembaren, Roga Florida; Chaidir, Chaidir; Sunaryanto, Rofiq; Wibowo, Agung Eru
Berita Biologi Vol 23 No 2 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2024.3334

Abstract

Andaliman (Zanthoxylum acanthopodium D.C) is a plant widely used by the Batak people in North Sumatera. Several studies demonstrated that Andaliman fruit exhibits antioxidant activities and cytotoxic effects on cancer cells. Quality of Andaliman fruit could affected by post-harvest process including storage time. This research aims to analyze the compound, antioxidant activity and proliferation inhibition on MCF-7 cells of Andaliman fruit extracts (AFE) stored at varying storage time. A series of fresh 250 g Andaliman fruit sealed in a closed container and stored in the oven at 30ºC. After storage at 0, 24, 48, 72, 96, 120 and 144 hours, Andaliman fruit macerated using methanol and obtained successive extract yields of 4.12; 3.70; 12.79; 8.17; 8.15; 3.7 and 2.21. These extracts were analyzed for total phenolic, total flavonoids, free radical scavenging activity and proliferation inhibition on MCF-7 cells. The chemical compound analysis of AFE was performed by ultra performance liquid chromatography (UPLC). Results showed that the AFE stored at zero hours had higher total phenol content (47.32 mg gallic acid equivalent (GAE)/g of extract) and free radical scavenging activity (50.38%) at 500 µg/mL significantly compared to other extracts. The total flavonoid content of all extracts showed no difference level except for the extract at 120 hours of storage. The proliferation inhibition test on MCF-7 cells at 100 µg/mL showed that AFE stored at 72, 96 and 144 hours could inhibit MCF-7 cells above 50%. The storage time variation of Andaliman fruit may affect the total phenolic and flavonoid content, and also activity of free radical scavenging and proliferation inhibition on MCF-7. The UPLC analysis founded the major compound of AFE was predicted as α-sanshool. Analysis of chemicals substance in Andaliman fruit with varying of storage time need to be conducted to evaluated alteration of secondary metabolites contained in Andaliman fruit.
PROTEASE FROM Styrax paralleloneurum LEAVES AND ITS THROMBOLYTIC ACTIVITY ASSAY Sinaga, Ester Rosdiana; Martgrita, Merry Meryam; Kembaren, Roga Florida
Berita Biologi Vol 24 No 2 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.9145

Abstract

Thrombotic disease is one of the leading causes of death worldwide and enzymatic thrombolytic therapy remains the best way to achieve recanalization. Enzymatic thrombolytic agents used today have undesirable side effects, and some are expensive. Research for herbal protease enzymes was conducted because plant drugs are known to be safer. This study aimed to obtain protease enzymes from frankincense leaves and to test their thrombolytic activity. Enzyme concentration was determined by the Bradford method, and the results showed that the highest enzyme concentration was 1.6812 g/μL at 40% ammonium sulfate fraction. A quantitative assay of protease activity using Folin & Ciocalteu's phenol reagent and casein as substrate showed that 1.2609 mg of enzyme hydrolyzed 32.5 mg of casein and released 0.0980 mg of L-tyrosine. The fibrinogenolytic activity of protease against human fibrinogen tested by SDS-PAGE showed that the protease could hydrolyze fibrinogen γ-chain within 0 minutes and β-chain within 60 minutes, while α-chain could not be hydrolyzed until 720 minutes. The inhibitor effect assay showed that the protease was a serine protease. This study concluded that the protease enzyme from frankincense leaves has the potential to be used as a thrombolytic agent. 
Co-Authors ADELINA MANURUNG Agnesia, Yoli Agung Eru Wibowo Ana Muliyana Andy Trirakhmadi Asido Saragih BRIAN SINAGA Chaidir Chaidir GRACE DOLOROSA LIMBONG Hutasoit, Sumy Ester Idah Rosidah Jaya Santoso LEVY NATHANAEL NABABAN Lianty Simangunsong Marselina Tan MEVA GUSTINA E. SIDAURUK Monita Pasaribu Nuralih Nuralih, Nuralih Putri, Theodora Mega Rahayu, Maya Damayanti Rofiq Sunaryanto ROGA FLORIDA KEMBAREN Sari Muthia Silalahi Sianipar, Imelda Febrika Simanungkalit, Grace Sophiwati Sinaga, Ester Rosdiana Siska Andrina Kusumastuti SURYA NINGSIH HUTAURUK Tambun, Rizka Arta Rossa Tan, Marselina Irasonia TURNIP, NINA JULIANA ROBERTA