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ROGA FLORIDA KEMBAREN
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The Role of the First 14 Amino Acids of Mature M1 Protein of Streptococcus pyogenes on Fibronectin-Binding Activity and Dimer Formation ROGA FLORIDA KEMBAREN; ADAM REZA GANJARA; VALENTINA YURINA; DEBBIE SOEFIE RETNONINGRUM
Microbiology Indonesia Vol. 4 No. 1 (2010): April 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1672.376 KB) | DOI: 10.5454/mi.4.1.5

Abstract

Streptococcus pyogenes is one of the most important human pathogens which express a multi-facet of virulence factors on its cell surface. One of the virulence factors that has been intensively-studied is the M protein that binds several human proteins. M1 protein, a member of the M protein family, was previously found to bind human fibronectin (Fn), an activity that is responsible for bacterial internalization. A structural study showed that this protein consists of four regions: A, B, S, and C. The study was intended to investigate the role of the first 14 amino acid residues located at the non-helical region of M1 protein in binding Fn, and its ability to form a dimer. The DNA fragment encoding for the ABS protein lacking its first 14 amino acids (ABSD14aa) was cloned into pET-16b, overexpressed in Escherichia coli BL21(DE3), and the protein was purified by affinity chromatography. The purified protein was characterized by sodium dodecyl sulphate polyacrylamide gel electrophoresis and the Fn-binding activtiy was assayed by enzyme linked immunosorbent assay. The result indicated that the M1 lacking its first 14 amino acids retains its dimerization and Fn-binding activities.
Peningkatan Literasi STEM melalui Del Mathematics and Science Competition Bidang Kimia Arianti, Santi Febri; Kembaren, Roga Florida; Ronauli Silalahi, Fitriani Tupa; Gurning, Kartini Florensya; Sihombing, Lasro Setia
Lebah Vol. 18 No. 2 (2025): Maret: Pengabdian
Publisher : IHSA Institute

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Abstract

Kompetisi sains dan matematika memiliki peran penting dalam mendukung literasi STEM (Science, Technology, Engineering, and Mathematics), terutama di wilayah dengan akses pendidikan terbatas. Artikel ini menganalisis Del Mathematics and Science Competition (DMSC) bidang kimia, yang menggunakan format kompetisi hibrida untuk menjangkau siswa dari berbagai latar belakang geografis di Sumatra Utara. Kegiatan ini dirancang untuk meningkatkan pemahaman konsep kimia seperti struktur atom, ikatan kimia, dan termodinamika, sekaligus memotivasi siswa untuk melanjutkan pendidikan di bidang STEM. Dari 485 peserta yang mendaftar, 100 siswa mengikuti kompetisi bidang kimia pada putaran awal daring, dan 25 siswa terbaik melanjutkan ke putaran final luring. Hasil menunjukkan peningkatan rata-rata nilai peserta dari 50,5 menjadi 70,2, mencerminkan efektivitas kompetisi dalam meningkatkan pemahaman materi. Survei juga mencatat peningkatan minat siswa terhadap STEM, dari 60% sebelum kompetisi menjadi 85% setelah kegiatan. Temuan ini mendukung efektivitas format hibrida yang inklusif dalam memberikan pengalaman belajar bermakna dan mengatasi tantangan aksesibilitas pendidikan. Saran untuk pengembangan kegiatan ke depan mencakup peningkatan dukungan teknologi dan penambahan komponen pelatihan untuk memperkuat dampak kompetisi
Co-Authors ADAM REZA GANJARA Arianti, Santi Febri DEBBIE SOEFIE RETNONINGRUM Fitriani Tupa R Silalahi Gurning, Kartini Florensya Sihombing, Lasro Setia VALENTINA YURINA