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Active Sites Determination of Enzyme 1-aminocyclopropane-1-carboxylic acid synthase 2 (ACS2) of Capsicum chinense using Modeling and In Silico Docking Pahlevi, Muhammad Rizza; Widhianata, Hani; Puspaningsih, Eunike Sri; Lestari, Juniarti Wulan; Sudibya, Eka; Suwasono, Prayitno Ribut; Habiburrahman , Novan
⁠International Journal of Sustainable Social Culture, Science Technology, Management, and Law Humanities Vol. 1 No. 2 (2024)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/d1yzr175

Abstract

The growth of Chili pepper (Capsicum chinense Jacquin.) is affected by biotic and abiotic stresses. Abiotic stress such as waterlogging increases the expression of ACS (1-aminocyclopropane-1-carboxylic acid synthase) enzyme. This enzyme plays a great role in the process of ethylene biosynthesis and encoded by multiple genes. Waterlogging causes hypoxia condition. One of ACS enzymes that responds to hypoxia condition is ACS2. The different respond to hypoxia stress among plant was assumed to be caused by a different structure of the ACS2 enzyme. This study aimed to identify and confirm the active site of Capsicum chinense Jacquin ACS2  using modeling and in silico docking. The result of the three-dimensional (3D) structure modeling showed 91% similarity of Capsicum chinense Jacquin ACS2 with the structure of the tomato (Solanum lycopersicum L.). The Capsicum chinense ACS2 confirmed five active sites that bind to the substrates asparagin396B, valine397B, thyrosine152B, threonine128B, and thyrosine92A. 
Exploring Water Pollution Subtopics: Validation of Histological Preparations Pahlevi, Muhammad Rizza; Habiburrahman , Novan; Zubdatu Zahrati; Lestariningsih; Infantri Humau
⁠International Journal of Sustainable Social Culture, Science Technology, Management, and Law Humanities Vol. 1 No. 1 (2024)
Publisher : Universitas Kristen Cipta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71131/8werny30

Abstract

This study aims to enhance understanding of water pollution through the use of histology preparations as educational tools for the sub-topic of water pollution in Grade X high school or equivalent. The research employs a descriptive method with a skinative approach. Water pollution is defined as the introduction of organisms, substances, energy, and/or other components into water due to human activities, resulting in a decline in water quality that affects its intended function. Histology preparations serve as a medium to assess the condition of organisms' tissues in rivers. Histology is essential for studying the normal tissue structure of organs or other body parts, addressing both anatomical and physiological aspects. Under a microscope, tissue preparations allow for the study of normal and abnormal tissue structures. Quality preparations must meet two criteria: identification, from organ collection through to observation, requiring expert handling; and cleanliness, assessed by the absence of air bubbles and the overall cleanliness of the preparation.