Garuda - Garba Rujukan Digital

COMPARISON OF ABTS (2,2-AZINOBIS(3-ETHYLBENZOTHIAZOLINE)-6-SULFONIC ACID) AND DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZYL) ASSAYS TO MEASURE THE ANTIOXIDANT ACTIVITY OF ETANOL EXTRACT OF RED ALGA (Gelidium sp) Martiani, Isye; Rustamsyah, Ardi; Puspitasari, Tien Ellita Endah; Sujana, Dani
Jurnal Ilmiah Farmako Bahari Vol 15, No 1 (2024): Jurnal Ilmiah Farmako Bahari
Publisher : Faculty of Mathematic and Natural Science, Garut University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jifb.v15i1.3461

Seaweeds have an ecological function as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar, and carrageenan) used in various food and pharmaceutical industries. This study aims to determine the antioxidant activity of red algae (Gelidium sp). Extraction was done using the maceration method using 96% ethanol and concentrated by rotary evaporation. The antioxidant activity of the extract was tested using ABTS and DPPH methods. Antioxidant activity was seen from the IC50. The results showed that IC50 from ethanol extract of red algae (Gelidium sp) with the DPPH method is 3,9154 ppm. Then, using the ABTS method, IC50 from ethanol extract of red algae (Gelidium sp) is 9,1178 ppm. This result shows that the ethanol extract from red algae (Gelidium sp) has very strong antioxidant activity (<50 ppm).

Pengaruh metode ekstraksi biji kewer roasting dan komponen senyawa aktifnya Mariani, Ria; Martiani, Isye; Noviyanti, Noviyanti; Avini, Fitni
Jurnal Farmasi & Sains Indonesia Vol 7 No 2 (2024)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52216/jfsi.vol7no2p92-99

Cassia occidentalis Linn. or kewer is a plant that is commonly found in tropical and subtropical climates. Cassia occidentalis seed extract is safe for consumption after roasting the seeds and extracting them using a water solvent. Drink from the roasted seeds of this plant is known as Negro coffee or Senna coffee. In Indonesia, including the Garut area, there are communities who usually consume boiled Cassia occidentalis seeds that have been roasted as a refreshing drink. It calls kewer tea. Cassia occidentalis seeds contain phenolic compounds. Phenol compounds are one of the secondary metabolites that have antioxidant activity. Behalf of that this research aims to determine the antioxidant activity of steeping, infusion and decoction of roasted Cassia occidentalis seed powder and to analysis the active compound content of three extracts. Antioxidant activity was tested using DPPH (2,2-diphenyl-1-picrylhidrazil) method, and active compounds were identified using LC-MS/MS analyses. The results of the antioxidant activity test showed that the roasted kewer seed steeping had the highest antioxidant activity compared to the infusion and decoction, with an IC50 value of 57.864 µg/ml. The results of compound content analysis using LC-MS/MS indicated 13 compounds in the steeping, 15 compounds in the infusion, and 16 compounds in the decoction. It is suspected that compounds that play a role in antioxidant activity in steeping include flavonoids, lignans, and stilbenes, which are lost or reduced in levels in infusions and decoctions.

Peningkatan Kualitas Produk Kunyit Asam Instan Produksi Kober Nurul Huda: Quality Improvement of Kunyit Asam Instant Drink Manufactured by Kober Nurul Huda Syamsudin, Raden Aldizal Mahendra Rizkio; Sriarumtias, Framesti Frisma; Kurniawan, Deri Alan; Martiani, Isye
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 10 No. 2 (2025): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v10i2.8244

Kober Nurul Huda was an early childhood education institution located at the foot of Mount Cikuray, in Dangiang Village, Garut Regency, West Java. To support the institution's economy, an innovation was needed to generate additional income, one of which was through the utilization of local plants into products. Turmeric was a plant that could be processed into various products, one of which was the traditional turmeric tamarind drink. The method used in this community service activity was a participatory approach through observation, training, assistance, and evaluation of the results. Training and assistance were provided to improve the quality of instant turmeric tamarind products. A questionnaire was given to 26 respondents, consisting of pre and post-tests, to assess participants' knowledge. A 33% increase in understanding was achieved. Furthermore, assistance was provided in packaging and product licensing. Attractive product packaging was developed, and an SPP-IRT certificate was obtained. Overall, the partners were satisfied with this activity.

COMPARISON OF ABTS (2,2-AZINOBIS(3-ETHYLBENZOTHIAZOLINE)-6-SULFONIC ACID) AND DPPH (1,1-DIPHENYL-2-PICRYLHYDRAZYL) ASSAYS TO MEASURE THE ANTIOXIDANT ACTIVITY OF ETANOL EXTRACT OF RED ALGA (Gelidium sp) Martiani, Isye; Rustamsyah, Ardi; Puspitasari, Tien Ellita Endah; Sujana, Dani
Jurnal Ilmiah Farmako Bahari Vol 15 No 1 (2024): Jurnal Ilmiah Farmako Bahari
Publisher : Faculty of Mathematic and Natural Science, Garut University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jifb.v15i1.3461

Seaweeds have an ecological function as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar, and carrageenan) used in various food and pharmaceutical industries. This study aims to determine the antioxidant activity of red algae (Gelidium sp). Extraction was done using the maceration method using 96% ethanol and concentrated by rotary evaporation. The antioxidant activity of the extract was tested using ABTS and DPPH methods. Antioxidant activity was seen from the IC50. The results showed that IC50 from ethanol extract of red algae (Gelidium sp) with the DPPH method is 3,9154 ppm. Then, using the ABTS method, IC50 from ethanol extract of red algae (Gelidium sp) is 9,1178 ppm. This result shows that the ethanol extract from red algae (Gelidium sp) has very strong antioxidant activity (<50 ppm).

Pemberdayaan tukang jamu gendong kabupaten garut melalui penerapan CPOTB skala rumah tangga Kokom, Asep; Mariani, Ria; Amalia, Nur; Martiani, Isye; Fadhlillah, Faizah Min; Maharani, Raisha; Fajarwati, Neng Nabila
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 9, No 6 (2025): November
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v9i6.34259

Abstrak Pengobatan tradisional telah menjadi bagian penting dari budaya Indonesia, dengan jamu sebagai salah satu warisan kesehatan yang masih dilestarikan hingga kini. Jamu gendong, yang diproduksi dan dipasarkan secara keliling oleh penjual, banyak dijumpai di Kabupaten Garut. Obat tradisional termasuk jamu gendong dalam proses pembuatannya harus memenuhi standar keamanan dan mutu produk sebagaimana diatur dalam Cara Pembuatan Obat Tradisional yang Baik (CPOTB). Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberdayakan penjual jamu gendong di Kabupaten Garut melalui penerapan CPOTB skala rumah tangga. Evaluasi pengetahuan dilakukan menggunakan pre-test dan post-test mencakup lima aspek CPOTB: pengertian dan tujuan, bahan baku, produksi, peralatan, dan higienitas personal. Hasil menunjukkan adanya peningkatan rata-rata pengetahuan peserta dari 87,5% sebelum peyuluhan menjadi 100% setelah penyuluhan. Peningkatan ini mencerminkan efektivitas kegiatan dalam meningkatkan pemahaman CPOTB. Namun, hasil diskusi mengungkapkan bahwa pemahaman tersebut belum sepenuhnya diimplementasikan dalam praktik produksi. Oleh karena itu, diperlukan pelatihan lanjutan berbasis praktik dan pendampingan berkelanjutan agar penerapan CPOTB dapat berjalan secara konsisten. Kata kunci: CPOTB; jamu gendong; Kabupaten Garut; mutu AbstractTraditional medicine has been an important part of Indonesian culture, with jamu as one of the health heritages that is still preserved today. Jamu gendong, which is produced and sold door-to-door by vendors, is widely found in Kabupaten Garut. Traditional medicines, including jamu gendong, must be produced in compliance with the safety and quality standards set out in the Good Traditional Medicine Manufacturing Practices (CPOTB). This community service activity aimed to empower jamu gendong vendors in Kabupaten Garut through the implementation of household-scale CPOTB. Knowledge evaluation was conducted using pre-test and post-test assessments covering five aspects of CPOTB: definition and objectives, raw materials, production, equipment, and personal hygiene. The results showed an increase in the participants’ average knowledge from 87.5% before the training to 100% after the training. This improvement reflects the effectiveness of the program in enhancing participants’ understanding of CPOTB. However, discussions revealed that this knowledge has not been fully implemented in production practices. Therefore, further practice-based training and continuous mentoring are needed to ensure the consistent application of CPOTB. Keywords: CPOTB; jamu gendong; Kabupaten Garut; quality

UJI AKTIVITAS ANTIHIPERURISEMIA EKSTRAK ETANOL KULIT BUAH SUKUN (Artocarpus altilis (Parkinson) Fosberg) PADA MENCIT PUTIH JANTAN GALUR SWISS WEBSTER Renggana, Hesti; Sujana, Dani; Martiani, Isye; Amalia, Nur; Rantika, Nopi; Hindun, Siti; Rachyani, Evitri Risa
Jurnal Buana Farma Vol 5 No 4 (2025): Jurnal Buana Farma
Publisher : Fakultas Farmasi Universitas Buana Perjuangan Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36805/jbf.v5i4.1576

Hyperuricemia is defined as an elevated serum uric acid level beyond the normal physiological range, which may result from increased purine metabolism or impaired renal excretion of uric acid. Long-term pharmacological management of hyperuricemia can lead to adverse effects, highlighting the need for alternative treatments derived from natural sources. One such alternative is the skin of Artocarpus altilis, which is rich in flavonoids. The objective of this study was to evaluate the antihyperuricemic activity of ethanol extract from Artocarpus altilis fruit skin in male mice induced with potassium oxonate and chicken liver extract. Serum uric acid levels were quantified using the Point of Care Testing (POCT) method with an Easy Touch® digital device. The doses administered were 100, 200, and 400 mg/kgBW, with 13 mg/BW of Allopurinol as a positive control. Statistical analysis was conducted using ANOVA followed by post-hoc LSD tests. The results indicated that the ethanol extract of breadfruit skin exhibited significant antihyperuricemic activity at all tested doses. A dose of 100 mg/kgBW resulted in a 40.72% reduction in serum uric acid levels, with an efficacy of 68.33%. The observed effect is likely attributed to the flavonoid content, which inhibits xanthine oxidase, thereby making the 100 mg/kgBW dose the most effective in reducing uric acid levels.

Title

Found 6 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 6 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 6 Documents
Search