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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Nommensen Journal of Medicine Jurnal Kedokteran Ibnu Nafis Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara
Sitanggang, Ervina Julien
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Encapsulated Mesenchymal Stem Cell as Regenerative Alternative for MDR-TB: A Gene Expression Analysis of ABCG2 Efflux Sibuea, Christine Verawaty; Marpaung, Dina Octafrida; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Masal, Cindy Destasya; Laia, George Azriel Buala Nama; Tambunan, Octora Angelica Violyn; Samosir, Wanda Lucia; Sidabalok, Cici Kres
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.12040

Abstract

Multidrug-resistant tuberculosis (MDR-TB) poses a significant global health challenge due to poor treatment outcomes. Encapsulated mesenchymal stem cells (MSCs) have emerged as a potential alternative therapy; however, their role in modulating bacterial drug sensitivity remains unclear. This study aimed to evaluate the expression of the ABCG2 efflux pump gene in encapsulated MSCs co-cultured with MDR-TB, and to explore its implications for bacterial sensitivity to anti-tuberculosis drugs. An in vitro experimental design was employed using encapsulated MSCs cultured with MDR-TB. Total RNA was isolated, converted into complementary DNA (cDNA), and analyzed using quantitative real-time PCR (RT-PCR). Gene expression levels were quantified using the Livak (ΔΔCt) method. Results demonstrated a progressive increase in ABCG2 gene expression on days 2, 7, and 14. Although this increase may reduce the direct antibacterial capacity of MSCs, previous studies have shown that their preserved paracrine function remains beneficial for immunomodulation. These findings support the continued investigation of encapsulated MSCs as an adjunctive therapy for MDR-TB, particularly through immunoregulatory mechanisms despite increased ABCG2 expression.
Co-Authors Christine Verawaty Sibuea Christine Verawaty Sibuea Dalimunthe, Puput Novianty Halawa, Karina Mutiarani Hasibuan, Pantas Jenny Ria Sihombing Joseph Partogi Sibarani Laia, George Azriel Buala Nama Marpaung, Dina Octafrida Masal, Cindy Destasya Napitupulu, Runggu Novreka Pratiwi Sipayung Ompusunggu, Henny Erina Saurmauli pakpahan, reni Ristarin Paskarina Zaluchu Saharnauli Janna Verawaty Simorangkir Samosir, Wanda Lucia Sibarani, Maria Manuella Sidabalok, Cici Kres Simaremare, Ade P Siregar, Betania Narwastu Sitanggang, Tiur Maria Carolina BR Sufida, Sufida Tambunan, Octora Angelica Violyn Zaluchu, Ristarin