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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Science Midwifery Nommensen Journal of Medicine Jurnal Kedokteran Ibnu Nafis Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara
Sitanggang, Ervina Julien
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Veterinary

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Encapsulated Mesenchymal Stem Cell as Regenerative Alternative for MDR-TB: A Gene Expression Analysis of ABCG2 Efflux Sibuea, Christine Verawaty; Marpaung, Dina Octafrida; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Masal, Cindy Destasya; Laia, George Azriel Buala Nama; Tambunan, Octora Angelica Violyn; Samosir, Wanda Lucia; Sidabalok, Cici Kres
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.12040

Abstract

Multidrug-resistant tuberculosis (MDR-TB) poses a significant global health challenge due to poor treatment outcomes. Encapsulated mesenchymal stem cells (MSCs) have emerged as a potential alternative therapy; however, their role in modulating bacterial drug sensitivity remains unclear. This study aimed to evaluate the expression of the ABCG2 efflux pump gene in encapsulated MSCs co-cultured with MDR-TB, and to explore its implications for bacterial sensitivity to anti-tuberculosis drugs. An in vitro experimental design was employed using encapsulated MSCs cultured with MDR-TB. Total RNA was isolated, converted into complementary DNA (cDNA), and analyzed using quantitative real-time PCR (RT-PCR). Gene expression levels were quantified using the Livak (ΔΔCt) method. Results demonstrated a progressive increase in ABCG2 gene expression on days 2, 7, and 14. Although this increase may reduce the direct antibacterial capacity of MSCs, previous studies have shown that their preserved paracrine function remains beneficial for immunomodulation. These findings support the continued investigation of encapsulated MSCs as an adjunctive therapy for MDR-TB, particularly through immunoregulatory mechanisms despite increased ABCG2 expression.
TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Silaen, Rachel Teodora; Kuara , Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora; Yana, Hiqmah Yusi; Pratama, Gita; Mutiara, Mutiara; Angeline, Wiedya Kristianti
Science Midwifery Vol 11 No 6 (2024): February: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i6.1427

Abstract

Mesenchymal stem cells (MSCs) have been used as a cellular therapy for infectious and degenerative diseases due to their paracrine effect, immunomodulatory capability, high ability differentiation, and high plasticity. The paracrine effect of MSCs releases many growth factors and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), enabling them to modulate the immune system. Nevertheless, there are many obstacles to maintaining paracrine effects in cellular therapy due to a shortage of cellular retention. MSC encapsulation provides a favourable environment for the enhanced viability of MSCs. Platelet lysate is comprised of many growth factors that support the paracrine effect of mesenchymal stem cells (MSCs). In this study, MSCs were encapsulated within alginate, crosslinked using calcium chloride (CaCl2), and subsequently coated with platelet lysate. Encapsulated MSCs coated by platelet lysate were cultured for 21 days and analyzed for IL-10 and TNF-α levels. The findings of our study performed that TNF-α in encapsulated mesenchymal stem cells (MSCs) coated with platelet lysate increased until day 21. IL-10 was retained within the capsule and detected very in day 14. This study showed that encapsulated MSCs coated with platelet lysate affected paracrine effect TNF-α of MSC and retained IL-10 inside the capsule
Co-Authors Angeline, Wiedya Kristianti Christine Verawaty Sibuea Christine Verawaty Sibuea Dalimunthe, Puput Novianty Ginting, Kharnis Marsha Madora Gita Pratama Halawa, Karina Mutiarani Hasibuan, Pantas Jenny Ria Sihombing Joseph Partogi Sibarani Kuara , Glenessa Laia, George Azriel Buala Nama Marpaung, Dina Octafrida Masal, Cindy Destasya mutiara mutiara Napitupulu, Runggu Novreka Pratiwi Sipayung Ompusunggu, Henny Erina Saurmauli pakpahan, reni Ristarin Paskarina Zaluchu Saharnauli Janna Verawaty Simorangkir Samosir, Sarah Christina Samosir, Wanda Lucia Sibarani, Maria Manuella Sidabalok, Cici Kres Silaen, Rachel Teodora Simaremare, Ade P Siregar, Betania Narwastu Sitanggang, Tiur Maria Carolina BR Sufida, Sufida Tambunan, Octora Angelica Violyn Yana, Hiqmah Yusi Zaluchu, Ristarin